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The map label for this gene is wecB [C]

Identifier: 189424564

GI number: 189424564

Start: 1574546

End: 1575691

Strand: Reverse

Name: wecB [C]

Synonym: Glov_1505

Alternate gene names: 189424564

Gene position: 1575691-1574546 (Counterclockwise)

Preceding gene: 189424565

Following gene: 189424563

Centisome position: 40.22

GC content: 45.9

Gene sequence:

>1146_bases
GTGAAGATTGTAACCATCATCGGGGCACGCCCGCAATTTATCAAGGCAGCTGCCGTCAGCCGTTCCATTGCGGCTCATAT
CGCAGCGCGAAACGATCAACAATCCTCAATTAGCGAAATTATTGTTCACACTGGCCAGCATTTTGATGCCAATATGTCAG
ACATCTTCTTTGAAGAAATGGATATACCAAAGCCTGACTATCACCTTGCAATAAACTCGCTCAGCCATGGCGCTATGACT
GGCAGAATGCTGGAGAAAATCGAAGAAGTACTGCTTAAGGAAAAGCCTGATTATGTGCTTGTCTATGGTGATACTAATTC
TACCCTTGCCGGAGCATTGGCAGCAAGAAAGCTCCATATCAAGGTTGTGCATGTGGAAGCAGGGCTCCGTAGTTTCAATA
TGAATATGCCTGAAGAGATTAACCGTATCCTCACCGACCGGATCAGCGACATCCTCTGCTGCCCGACTGAAACAGCAGTT
AAAAATCTTGTTGCCGAAGGTTTTCAAAATAGCGACAAGCTGCTATCAGCAAATAGCGTACCGCTGGTTGTCAACACAGG
AGATGTAATGTATGACGCCGCCTTACATTACAGTACGTTTTCTGCTGAACGAAGTACCGTTATGAAAGGCTTGAATTTGA
AAGGCCGGAAGTTTGTTCTCTGCACCATCCATCGTGCTGAAAATACTGATGATCCAGATCGGTTGAAAAGCATTTTCAGA
GCTCTGGTAGAAATCAACAAAGACATGCGTGTAGTAGTGCCTTTGCACCCGCGCACACGCAATATACTTACGACTGTATT
CAGCGGTTCTGATGCACTCGCTATAATCAACGATGCTCTGACACTACTGGACCCGGTTGGTTATTTTGACATGGTTGAGC
TGCTGAAACATGCGACGATGGTTATGACTGACAGTGGCGGCTTACAAAAAGAGGCCTATTTCTTTGGCAAGCCCTGTATC
ACCTTGCGTGATGAAACGGAGTGGGTCGAGCTGGTAGATAATGGCTTTAACATCTTGGCCAGTGCGGATTACATGCGGAT
ACTTGCATGTTTTCAGGAGATGTCGTCTAAAACAGTCGATGTCGATCGAGCACTATATGGCACGGGCGATGCTTCCAATA
AGATACTGAATCTCTTTCTACACTAA

Upstream 100 bases:

>100_bases
CACTGGCACAACGGGTCATAAGCCTGCCGATGCACGCGTATCTTGCAACAGATCAACAGATAGCTATCTCTACTACAGTC
GCTGGAGCTGGAGTTATAAA

Downstream 100 bases:

>100_bases
GCTGATCTTGCCCGATCAGAGTAGACATTCAGCTAAGCTGCTTTGAGAACCTCAAACGCTATTGTGCTGATACGACCAAT
TGCGTTGTGGCGTTGATTAC

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKIVTIIGARPQFIKAAAVSRSIAAHIAARNDQQSSISEIIVHTGQHFDANMSDIFFEEMDIPKPDYHLAINSLSHGAMT
GRMLEKIEEVLLKEKPDYVLVYGDTNSTLAGALAARKLHIKVVHVEAGLRSFNMNMPEEINRILTDRISDILCCPTETAV
KNLVAEGFQNSDKLLSANSVPLVVNTGDVMYDAALHYSTFSAERSTVMKGLNLKGRKFVLCTIHRAENTDDPDRLKSIFR
ALVEINKDMRVVVPLHPRTRNILTTVFSGSDALAIINDALTLLDPVGYFDMVELLKHATMVMTDSGGLQKEAYFFGKPCI
TLRDETEWVELVDNGFNILASADYMRILACFQEMSSKTVDVDRALYGTGDASNKILNLFLH

Sequences:

>Translated_381_residues
MKIVTIIGARPQFIKAAAVSRSIAAHIAARNDQQSSISEIIVHTGQHFDANMSDIFFEEMDIPKPDYHLAINSLSHGAMT
GRMLEKIEEVLLKEKPDYVLVYGDTNSTLAGALAARKLHIKVVHVEAGLRSFNMNMPEEINRILTDRISDILCCPTETAV
KNLVAEGFQNSDKLLSANSVPLVVNTGDVMYDAALHYSTFSAERSTVMKGLNLKGRKFVLCTIHRAENTDDPDRLKSIFR
ALVEINKDMRVVVPLHPRTRNILTTVFSGSDALAIINDALTLLDPVGYFDMVELLKHATMVMTDSGGLQKEAYFFGKPCI
TLRDETEWVELVDNGFNILASADYMRILACFQEMSSKTVDVDRALYGTGDASNKILNLFLH
>Mature_381_residues
MKIVTIIGARPQFIKAAAVSRSIAAHIAARNDQQSSISEIIVHTGQHFDANMSDIFFEEMDIPKPDYHLAINSLSHGAMT
GRMLEKIEEVLLKEKPDYVLVYGDTNSTLAGALAARKLHIKVVHVEAGLRSFNMNMPEEINRILTDRISDILCCPTETAV
KNLVAEGFQNSDKLLSANSVPLVVNTGDVMYDAALHYSTFSAERSTVMKGLNLKGRKFVLCTIHRAENTDDPDRLKSIFR
ALVEINKDMRVVVPLHPRTRNILTTVFSGSDALAIINDALTLLDPVGYFDMVELLKHATMVMTDSGGLQKEAYFFGKPCI
TLRDETEWVELVDNGFNILASADYMRILACFQEMSSKTVDVDRALYGTGDASNKILNLFLH

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=390, Percent_Identity=32.0512820512821, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 42311; Mature: 42311

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVTIIGARPQFIKAAAVSRSIAAHIAARNDQQSSISEIIVHTGQHFDANMSDIFFEEM
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHC
DIPKPDYHLAINSLSHGAMTGRMLEKIEEVLLKEKPDYVLVYGDTNSTLAGALAARKLHI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHEEEEEE
KVVHVEAGLRSFNMNMPEEINRILTDRISDILCCPTETAVKNLVAEGFQNSDKLLSANSV
EEEEEECCCHHCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCEEECCCC
PLVVNTGDVMYDAALHYSTFSAERSTVMKGLNLKGRKFVLCTIHRAENTDDPDRLKSIFR
EEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
ALVEINKDMRVVVPLHPRTRNILTTVFSGSDALAIINDALTLLDPVGYFDMVELLKHATM
HHHHCCCCCEEEEECCCCCCHHEEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHCEE
VMTDSGGLQKEAYFFGKPCITLRDETEWVELVDNGFNILASADYMRILACFQEMSSKTVD
EEECCCCCCCCEEEECCCEEEECCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCEE
VDRALYGTGDASNKILNLFLH
HHHHEECCCCCCCHHEEEEEC
>Mature Secondary Structure
MKIVTIIGARPQFIKAAAVSRSIAAHIAARNDQQSSISEIIVHTGQHFDANMSDIFFEEM
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHC
DIPKPDYHLAINSLSHGAMTGRMLEKIEEVLLKEKPDYVLVYGDTNSTLAGALAARKLHI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHEEEEEE
KVVHVEAGLRSFNMNMPEEINRILTDRISDILCCPTETAVKNLVAEGFQNSDKLLSANSV
EEEEEECCCHHCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCEEECCCC
PLVVNTGDVMYDAALHYSTFSAERSTVMKGLNLKGRKFVLCTIHRAENTDDPDRLKSIFR
EEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHH
ALVEINKDMRVVVPLHPRTRNILTTVFSGSDALAIINDALTLLDPVGYFDMVELLKHATM
HHHHCCCCCEEEEECCCCCCHHEEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHCEE
VMTDSGGLQKEAYFFGKPCITLRDETEWVELVDNGFNILASADYMRILACFQEMSSKTVD
EEECCCCCCCCEEEECCCEEEECCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHCCCCEE
VDRALYGTGDASNKILNLFLH
HHHHEECCCCCCCHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA