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The map label for this gene is insK [C]

Identifier: 189424496

GI number: 189424496

Start: 1478347

End: 1479156

Strand: Reverse

Name: insK [C]

Synonym: Glov_1432

Alternate gene names: 189424496

Gene position: 1479156-1478347 (Counterclockwise)

Preceding gene: 189424497

Following gene: 189424492

Centisome position: 37.76

GC content: 49.75

Gene sequence:

>810_bases
GTGCGGCATTTGCATCTGTCTTACGGTGTAAGCGAGCGCCGCAGCTGTGATGTGGTTCTCCTCTGCCGGGGATCATATCG
TTATGTTCATCACGGCAAAGACGATTCCGCCCTTCGGTTGCGAATTCGCCAGATAGCCGAGACGCGGATTCGTTATGGCT
ATCTTCGTATCCACACCCTGTTGCGACGTGAGGGCTGGCACGTCAATCACAAGCGGGTCTACCGGATATACTGTGAAGAA
TGCCTGAATCTGCGCCGTAAAAGACCGCGTAGAAGAGTTAGCGCGGCCCATCGAGCCAATCGGCCTGTCGCCTCTAGTTT
AAATGACTCTTGGAGTATGGATTTTGTTGCCGACAGCCTGTTCAATGGTCGGCGTTTCAGGGCCTTAACTGTAGTGGATA
ACTGGAGTCGCCAATGCCTTGCCATTCGAGTCGATCAGGCTATGAAAGGCGATGATGTGGTAGATGCAATGTCTGAACTG
ACGCAGATCCGTAACTGCCCGAAGCGGATTTTTCTGGACAACGGCAGTGAATTTATTTCTAAATCACTAGATCGCTGGGC
TTACGAGAACGGTGTCACGCTTGACTTCTCAAGACCTGGCAAACCCACTGATAATGCGCTGATAGAGTCATTTAATGGTA
GTTTTCGTGATGAGTGTTTGAGCGTTAACTGGTTTCTGTCAATGGACGATGCCAGACAAAAGATCGAAGACTGGCGACAG
GAATATAACGATTTCAGACCGCATACGGCACTGAAAAATCTGACGCCGAACGAGTATGCGAATCAGTTTCAAGAAGCCGG
AAATCTCTAA

Upstream 100 bases:

>100_bases
GGCAATTAAAGAAGCTGGTGGCCGATCTAAGTCTTGATAAGCAGATGCTTCAGGACGTTCTCTCAAAAAAGTTCTAAAGC
CAGCCCAGAGGCGAAATCTT

Downstream 100 bases:

>100_bases
TTTAGGCTGGAATAGTTTTCGGGTGGAGCTCAGTTTTGTGGTTGCAAATTGCAACAGTAGAAATGCTGTCGGGGCATTGA
CGTTACTGTCGACAATCTGT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRHLHLSYGVSERRSCDVVLLCRGSYRYVHHGKDDSALRLRIRQIAETRIRYGYLRIHTLLRREGWHVNHKRVYRIYCEE
CLNLRRKRPRRRVSAAHRANRPVASSLNDSWSMDFVADSLFNGRRFRALTVVDNWSRQCLAIRVDQAMKGDDVVDAMSEL
TQIRNCPKRIFLDNGSEFISKSLDRWAYENGVTLDFSRPGKPTDNALIESFNGSFRDECLSVNWFLSMDDARQKIEDWRQ
EYNDFRPHTALKNLTPNEYANQFQEAGNL

Sequences:

>Translated_269_residues
MRHLHLSYGVSERRSCDVVLLCRGSYRYVHHGKDDSALRLRIRQIAETRIRYGYLRIHTLLRREGWHVNHKRVYRIYCEE
CLNLRRKRPRRRVSAAHRANRPVASSLNDSWSMDFVADSLFNGRRFRALTVVDNWSRQCLAIRVDQAMKGDDVVDAMSEL
TQIRNCPKRIFLDNGSEFISKSLDRWAYENGVTLDFSRPGKPTDNALIESFNGSFRDECLSVNWFLSMDDARQKIEDWRQ
EYNDFRPHTALKNLTPNEYANQFQEAGNL
>Mature_269_residues
MRHLHLSYGVSERRSCDVVLLCRGSYRYVHHGKDDSALRLRIRQIAETRIRYGYLRIHTLLRREGWHVNHKRVYRIYCEE
CLNLRRKRPRRRVSAAHRANRPVASSLNDSWSMDFVADSLFNGRRFRALTVVDNWSRQCLAIRVDQAMKGDDVVDAMSEL
TQIRNCPKRIFLDNGSEFISKSLDRWAYENGVTLDFSRPGKPTDNALIESFNGSFRDECLSVNWFLSMDDARQKIEDWRQ
EYNDFRPHTALKNLTPNEYANQFQEAGNL

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=241, Percent_Identity=27.3858921161826, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786462, Length=157, Percent_Identity=30.5732484076433, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790724, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789424, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789224, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788305, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787669, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786558, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788405, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787263, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786753, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786570, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786490, Length=229, Percent_Identity=25.764192139738, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31719; Mature: 31719

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLHLSYGVSERRSCDVVLLCRGSYRYVHHGKDDSALRLRIRQIAETRIRYGYLRIHTL
CCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRREGWHVNHKRVYRIYCEECLNLRRKRPRRRVSAAHRANRPVASSLNDSWSMDFVADSL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHH
FNGRRFRALTVVDNWSRQCLAIRVDQAMKGDDVVDAMSELTQIRNCPKRIFLDNGSEFIS
CCCCEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHH
KSLDRWAYENGVTLDFSRPGKPTDNALIESFNGSFRDECLSVNWFLSMDDARQKIEDWRQ
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHEEEEEEEECHHHHHHHHHHHH
EYNDFRPHTALKNLTPNEYANQFQEAGNL
HHHCCCCHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRHLHLSYGVSERRSCDVVLLCRGSYRYVHHGKDDSALRLRIRQIAETRIRYGYLRIHTL
CCEEEECCCCCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRREGWHVNHKRVYRIYCEECLNLRRKRPRRRVSAAHRANRPVASSLNDSWSMDFVADSL
HHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHH
FNGRRFRALTVVDNWSRQCLAIRVDQAMKGDDVVDAMSELTQIRNCPKRIFLDNGSEFIS
CCCCEEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHH
KSLDRWAYENGVTLDFSRPGKPTDNALIESFNGSFRDECLSVNWFLSMDDARQKIEDWRQ
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHCCCCHHHHHEEEEEEEECHHHHHHHHHHHH
EYNDFRPHTALKNLTPNEYANQFQEAGNL
HHHCCCCHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]