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The map label for this gene is trmJ [H]

Identifier: 189424323

GI number: 189424323

Start: 1305185

End: 1305922

Strand: Reverse

Name: trmJ [H]

Synonym: Glov_1258

Alternate gene names: 189424323

Gene position: 1305922-1305185 (Counterclockwise)

Preceding gene: 189424324

Following gene: 189424322

Centisome position: 33.33

GC content: 59.21

Gene sequence:

>738_bases
ATGCCGCCATCCCTTACCGGTCTTTCCGTCATCCTTGTTGAACCCCAGTTTCCGGGCAATATCGGCATGGTCTGCCGTGC
CATGAAAAACATGGGGCTTTCACGCCTGCGCCTGGTGGCCGGCTGCGACCACCTCCACCCCGAGGCATTTAAATTTGCGG
TCTCAGCTAGAGATTTGCTGGAGCAGGCAGAACTGTTCGACAGTCTGGCAGACGCCCTGGCAGATATCAGCGTCTCAGTG
GCAACCACACGGCGCAGCGGCAAATACCGCCAGGAACTGCTCAGCCCCCCCCAAGCGGCCCAGGCACTGTTACAAGCGCC
AGGTTCTGCAGCGCTGGTCTTCGGACGTGAAGACCACGGACTTTCCACGGCAGACCTCTCCCTCTGCACACTGCAGGCGA
CCATACCGTCGAGTTCCGAGTACGGTTCCCTCAACCTCGCCCAGGCAGCCCTGATTTTCTGCTATGAGCTGTTCGGTGCT
GCCACCGCCGAGACCGGCTCTCTGCGACGGACACCGGCACCATCCGGAGAACTTGAACCGCTGTTCACCCACATGGAGCA
GACCCTGCTGCGCATTGGGCATTTGAACCCCCAAAATCCCGGTCACATCATGCGTTCGCTACGCCGCATATTTTTCAGGT
CCGGGCTTGACAGCCGCGAAGTTGCTATTCTGCGTGGCATGCTGTCCCAGATAGACTGGGCAGCCGGTGACTTCAACGAC
CGGAAAAAAACGACATGA

Upstream 100 bases:

>100_bases
TGTCTGCCCAGGCCCCTGACATTGACCCGGAAGCAAAAACTGAAGAGATGATTGATCTGATGCGCTCCGAAGTTGCCGGA
CGTCTGTCCGTCTAGCCACC

Downstream 100 bases:

>100_bases
CTCCCACCCTGATCGGCCTGATCGCCGGACTGCTTACCAGTGTCGCAGCACTGCCGCAGGTTATCAAGACCTGGCGCAGC
CGCCATGCGCGGGATCTTTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPPSLTGLSVILVEPQFPGNIGMVCRAMKNMGLSRLRLVAGCDHLHPEAFKFAVSARDLLEQAELFDSLADALADISVSV
ATTRRSGKYRQELLSPPQAAQALLQAPGSAALVFGREDHGLSTADLSLCTLQATIPSSSEYGSLNLAQAALIFCYELFGA
ATAETGSLRRTPAPSGELEPLFTHMEQTLLRIGHLNPQNPGHIMRSLRRIFFRSGLDSREVAILRGMLSQIDWAAGDFND
RKKTT

Sequences:

>Translated_245_residues
MPPSLTGLSVILVEPQFPGNIGMVCRAMKNMGLSRLRLVAGCDHLHPEAFKFAVSARDLLEQAELFDSLADALADISVSV
ATTRRSGKYRQELLSPPQAAQALLQAPGSAALVFGREDHGLSTADLSLCTLQATIPSSSEYGSLNLAQAALIFCYELFGA
ATAETGSLRRTPAPSGELEPLFTHMEQTLLRIGHLNPQNPGHIMRSLRRIFFRSGLDSREVAILRGMLSQIDWAAGDFND
RKKTT
>Mature_244_residues
PPSLTGLSVILVEPQFPGNIGMVCRAMKNMGLSRLRLVAGCDHLHPEAFKFAVSARDLLEQAELFDSLADALADISVSVA
TTRRSGKYRQELLSPPQAAQALLQAPGSAALVFGREDHGLSTADLSLCTLQATIPSSSEYGSLNLAQAALIFCYELFGAA
TAETGSLRRTPAPSGELEPLFTHMEQTLLRIGHLNPQNPGHIMRSLRRIFFRSGLDSREVAILRGMLSQIDWAAGDFNDR
KKTT

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=236, Percent_Identity=38.5593220338983, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=39.6103896103896, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26549; Mature: 26418

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSLTGLSVILVEPQFPGNIGMVCRAMKNMGLSRLRLVAGCDHLHPEAFKFAVSARDLL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQAELFDSLADALADISVSVATTRRSGKYRQELLSPPQAAQALLQAPGSAALVFGREDHG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCC
LSTADLSLCTLQATIPSSSEYGSLNLAQAALIFCYELFGAATAETGSLRRTPAPSGELEP
CCHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
LFTHMEQTLLRIGHLNPQNPGHIMRSLRRIFFRSGLDSREVAILRGMLSQIDWAAGDFND
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
RKKTT
CCCCC
>Mature Secondary Structure 
PPSLTGLSVILVEPQFPGNIGMVCRAMKNMGLSRLRLVAGCDHLHPEAFKFAVSARDLL
CCCCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQAELFDSLADALADISVSVATTRRSGKYRQELLSPPQAAQALLQAPGSAALVFGREDHG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCC
LSTADLSLCTLQATIPSSSEYGSLNLAQAALIFCYELFGAATAETGSLRRTPAPSGELEP
CCHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
LFTHMEQTLLRIGHLNPQNPGHIMRSLRRIFFRSGLDSREVAILRGMLSQIDWAAGDFND
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
RKKTT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA