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The map label for this gene is zraS [H]

Identifier: 189424232

GI number: 189424232

Start: 1221283

End: 1222938

Strand: Reverse

Name: zraS [H]

Synonym: Glov_1167

Alternate gene names: 189424232

Gene position: 1222938-1221283 (Counterclockwise)

Preceding gene: 189424233

Following gene: 189424231

Centisome position: 31.22

GC content: 52.17

Gene sequence:

>1656_bases
ATGAGCGGATGCCCCAAGCAACAGGAAAACTGTCTTGCCTATGTTCGCTACATCAGGGAAAAGACCAACCAACTGCTGGG
AGTTATGGGCACCGAGCCGCTGCAGGAGGAAGAGCTGGACGATCATGCTCTGATCGAGCTTGATCCGATCGGCACGATTG
CCCAGTCATTCGGCCACATACTGGAGTATCTCAATGAAACCATCAATGAATTACAGGTAGCCAAGGATGAACTGCAGGCG
ATCTTTGATGCCACCGGGGTAGGAATTTCAATCATCGACCGCAACTTCCGTATCCTGCGCTGCAACGAAAAACAACGCAC
CCTGCTGCTGGCGCCGGAAGCTGAAGATGTTACAGGCTGTTATTGCTTTGATGCCTACGGCAACCGCAATGCACCGGTGG
AGCATTGTCCGGCCAAATACACCTTTGCCACCGGTCAACCGGCCGTTGCGCGGGAGATTGTCAAGCGAGGCAAGTATTTT
CAGGTTGCCACCACGCCGTTTGCACGTGATGAAAACAACAAGGTTACCACCGTCATCGAGGTATCACTTGATATCACAGA
AAAAAAGATGGCAGAACAATCGGAAAAAGAGCAACGTGATTTTTACCTGACAGAGAAGTCAAAGCTGGCAACAGTACTGG
AAAGCTTGTCAGAGGGATTACTGGTGGCTGGTACCAATGACCGGATCCTTTCGGTTAACCGTTCTGCAACCATGATCCTG
CAACGTGATGAAGCGGAGCTGATTGGCCTGGCTCTTACGGACCTCTTTCCTGTCATAAGCGTTGAGCTGACTGATCCGAT
GGAAATCATTCAAGGACTTGAGCTAACCTGCACGCAGGCTCGTGCAAACGAATGCCACCTATCACTCAACATGGCCCCCT
TGCTGGATAATGAAATGAAACGGATCGGCCGTGTGATCACCTTCAGAAACATTACCGAAGAGAAGAAACAGCAGGAACTG
TATCACCGCACTGAAAAGTTGTCGGCAATCGGGCAGCTCTCTGCCGGTGTTGCCCATGAACTGAACACCCCATTGGGAAG
CATCCTGGGATACGCCCGGCTGTTACTGAAACAGGAGAACCTGACCCAACAGCAGCGTGAACAGCTGGCAGTGATTGCGG
AGCAGGCCAAAAAGAGCGGCACCATCATCCAGGCCTTGCTCAACTTCTCCCGCCATGCCCAACCAACCCAGTGTCGCCTG
GACGCATGTGACCTGAATAGTGTCATTAAAAATGCCCTTTGCCTGCTTACCACTGAACTCAGCAAACGCAAGATCATCCT
GACCACTGACCTTGAGCTGATCCCGAAGGTCTGCGCCGACCCATGGGGAATTGAACAGGTAGTGCTGAATCTGGTGATGA
ATGCCCTGCAGGCGATTGGTAGTCAGGGAGAGATCCGTATTATTGTCAGACGTCAGCCAGAAGGGGTCAGACTTGAGGTG
CAGGATAGCGGCCCCGGCATCCCGGATGAAGTAAGAAGCCGCATCTTTGACCCATTTTTTACCACCAAGCCGTTTGGGGA
AGGAACCGGGCTGGGACTTTCCATCTGCGCAGGAATTGTGAATGAGAACGGCGGCAATATCGATATTGTCAAGTCAGACA
GCAGAGGCACGACCTTTGTTGTAATCCTGCCCTGCAAGGAGTCGGTCGATGCCTGA

Upstream 100 bases:

>100_bases
ACACTACCCGTACGGTTCCTGCTTCTGCTCAAAAGATGGGGCTGGCTGTCGAAGTCCGCAAGGTGGAAACCTGGTACGAG
ATCAGTATCGGCGGTAAATC

Downstream 100 bases:

>100_bases
TCCACTTATTCTCATTGTCGATGATGACCTGAGGATGCGGCAACTGATCAGGGATACTCTGGCAAGTGAGGGTATCAATG
CAGTCCTCTGCAGTGACGGC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MSGCPKQQENCLAYVRYIREKTNQLLGVMGTEPLQEEELDDHALIELDPIGTIAQSFGHILEYLNETINELQVAKDELQA
IFDATGVGISIIDRNFRILRCNEKQRTLLLAPEAEDVTGCYCFDAYGNRNAPVEHCPAKYTFATGQPAVAREIVKRGKYF
QVATTPFARDENNKVTTVIEVSLDITEKKMAEQSEKEQRDFYLTEKSKLATVLESLSEGLLVAGTNDRILSVNRSATMIL
QRDEAELIGLALTDLFPVISVELTDPMEIIQGLELTCTQARANECHLSLNMAPLLDNEMKRIGRVITFRNITEEKKQQEL
YHRTEKLSAIGQLSAGVAHELNTPLGSILGYARLLLKQENLTQQQREQLAVIAEQAKKSGTIIQALLNFSRHAQPTQCRL
DACDLNSVIKNALCLLTTELSKRKIILTTDLELIPKVCADPWGIEQVVLNLVMNALQAIGSQGEIRIIVRRQPEGVRLEV
QDSGPGIPDEVRSRIFDPFFTTKPFGEGTGLGLSICAGIVNENGGNIDIVKSDSRGTTFVVILPCKESVDA

Sequences:

>Translated_551_residues
MSGCPKQQENCLAYVRYIREKTNQLLGVMGTEPLQEEELDDHALIELDPIGTIAQSFGHILEYLNETINELQVAKDELQA
IFDATGVGISIIDRNFRILRCNEKQRTLLLAPEAEDVTGCYCFDAYGNRNAPVEHCPAKYTFATGQPAVAREIVKRGKYF
QVATTPFARDENNKVTTVIEVSLDITEKKMAEQSEKEQRDFYLTEKSKLATVLESLSEGLLVAGTNDRILSVNRSATMIL
QRDEAELIGLALTDLFPVISVELTDPMEIIQGLELTCTQARANECHLSLNMAPLLDNEMKRIGRVITFRNITEEKKQQEL
YHRTEKLSAIGQLSAGVAHELNTPLGSILGYARLLLKQENLTQQQREQLAVIAEQAKKSGTIIQALLNFSRHAQPTQCRL
DACDLNSVIKNALCLLTTELSKRKIILTTDLELIPKVCADPWGIEQVVLNLVMNALQAIGSQGEIRIIVRRQPEGVRLEV
QDSGPGIPDEVRSRIFDPFFTTKPFGEGTGLGLSICAGIVNENGGNIDIVKSDSRGTTFVVILPCKESVDA
>Mature_550_residues
SGCPKQQENCLAYVRYIREKTNQLLGVMGTEPLQEEELDDHALIELDPIGTIAQSFGHILEYLNETINELQVAKDELQAI
FDATGVGISIIDRNFRILRCNEKQRTLLLAPEAEDVTGCYCFDAYGNRNAPVEHCPAKYTFATGQPAVAREIVKRGKYFQ
VATTPFARDENNKVTTVIEVSLDITEKKMAEQSEKEQRDFYLTEKSKLATVLESLSEGLLVAGTNDRILSVNRSATMILQ
RDEAELIGLALTDLFPVISVELTDPMEIIQGLELTCTQARANECHLSLNMAPLLDNEMKRIGRVITFRNITEEKKQQELY
HRTEKLSAIGQLSAGVAHELNTPLGSILGYARLLLKQENLTQQQREQLAVIAEQAKKSGTIIQALLNFSRHAQPTQCRLD
ACDLNSVIKNALCLLTTELSKRKIILTTDLELIPKVCADPWGIEQVVLNLVMNALQAIGSQGEIRIIVRRQPEGVRLEVQ
DSGPGIPDEVRSRIFDPFFTTKPFGEGTGLGLSICAGIVNENGGNIDIVKSDSRGTTFVVILPCKESVDA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=249, Percent_Identity=36.5461847389558, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788549, Length=355, Percent_Identity=30.4225352112676, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1790300, Length=369, Percent_Identity=28.9972899728997, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994928, Length=370, Percent_Identity=25.6756756756757, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789149, Length=219, Percent_Identity=35.1598173515982, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082128, Length=265, Percent_Identity=26.7924528301887, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=29.9539170506912, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1786912, Length=217, Percent_Identity=31.3364055299539, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=28.0487804878049, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=27.3148148148148, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=215, Percent_Identity=28.8372093023256, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788713, Length=302, Percent_Identity=24.5033112582781, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=28.1818181818182, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=204, Percent_Identity=28.921568627451, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=27.8761061946903, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=25.9090909090909, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61111; Mature: 60980

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGCPKQQENCLAYVRYIREKTNQLLGVMGTEPLQEEELDDHALIELDPIGTIAQSFGHI
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCHHHHHHHHHHH
LEYLNETINELQVAKDELQAIFDATGVGISIIDRNFRILRCNEKQRTLLLAPEAEDVTGC
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCCCCEE
YCFDAYGNRNAPVEHCPAKYTFATGQPAVAREIVKRGKYFQVATTPFARDENNKVTTVIE
EEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
VSLDITEKKMAEQSEKEQRDFYLTEKSKLATVLESLSEGLLVAGTNDRILSVNRSATMIL
EEECCHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHCCEEEECCCCCEEEECCCEEEEE
QRDEAELIGLALTDLFPVISVELTDPMEIIQGLELTCTQARANECHLSLNMAPLLDNEMK
ECCCHHHHHHHHHHHHHHEEEEECCHHHHHCCCCEEEECCCCCEEEEEEECHHHHHHHHH
RIGRVITFRNITEEKKQQELYHRTEKLSAIGQLSAGVAHELNTPLGSILGYARLLLKQEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LTQQQREQLAVIAEQAKKSGTIIQALLNFSRHAQPTQCRLDACDLNSVIKNALCLLTTEL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHEECC
SKRKIILTTDLELIPKVCADPWGIEQVVLNLVMNALQAIGSQGEIRIIVRRQPEGVRLEV
CCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
QDSGPGIPDEVRSRIFDPFFTTKPFGEGTGLGLSICAGIVNENGGNIDIVKSDSRGTTFV
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
VILPCKESVDA
EEEECCCCCCC
>Mature Secondary Structure 
SGCPKQQENCLAYVRYIREKTNQLLGVMGTEPLQEEELDDHALIELDPIGTIAQSFGHI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCHHHHHHHHHHH
LEYLNETINELQVAKDELQAIFDATGVGISIIDRNFRILRCNEKQRTLLLAPEAEDVTGC
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCCEEEECCCCCCCCEE
YCFDAYGNRNAPVEHCPAKYTFATGQPAVAREIVKRGKYFQVATTPFARDENNKVTTVIE
EEEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEE
VSLDITEKKMAEQSEKEQRDFYLTEKSKLATVLESLSEGLLVAGTNDRILSVNRSATMIL
EEECCHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHCCEEEECCCCCEEEECCCEEEEE
QRDEAELIGLALTDLFPVISVELTDPMEIIQGLELTCTQARANECHLSLNMAPLLDNEMK
ECCCHHHHHHHHHHHHHHEEEEECCHHHHHCCCCEEEECCCCCEEEEEEECHHHHHHHHH
RIGRVITFRNITEEKKQQELYHRTEKLSAIGQLSAGVAHELNTPLGSILGYARLLLKQEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LTQQQREQLAVIAEQAKKSGTIIQALLNFSRHAQPTQCRLDACDLNSVIKNALCLLTTEL
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHEECC
SKRKIILTTDLELIPKVCADPWGIEQVVLNLVMNALQAIGSQGEIRIIVRRQPEGVRLEV
CCCEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
QDSGPGIPDEVRSRIFDPFFTTKPFGEGTGLGLSICAGIVNENGGNIDIVKSDSRGTTFV
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEE
VILPCKESVDA
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]