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The map label for this gene is tnpR [H]

Identifier: 189424197

GI number: 189424197

Start: 1183910

End: 1184464

Strand: Reverse

Name: tnpR [H]

Synonym: Glov_1132

Alternate gene names: 189424197

Gene position: 1184464-1183910 (Counterclockwise)

Preceding gene: 189424198

Following gene: 189424196

Centisome position: 30.23

GC content: 54.41

Gene sequence:

>555_bases
ATGGCAATCATCGGATATGCACGGGTAAGCACAGAAGGCCAGGAACTGACCGCACAGCTTGAGCAACTAAACGGTGCTGG
TGTTGATAAGATCTTCAAAGAAAAAGCATCAGGCGTGAAGCAAGACCGCCCACAGCTGGCGGCAATGCTTGACCACCTCC
GGGAAGGTGACACGGTAATAGTCTGCAAGCTGGACCGGATAGCCCGCAGCACCAAACACCTGTTAGAGATCGTAGAGTCA
CTTGAAGCCAGAAAGGTTTCCTTCAAGGTCTTGAACATCAACCTGGACACCTCCACCCCCACCGGCAAGCTGATGCTTTC
CATGCTGGCATCAATCGCACAGTTTGAAAGGGAAATGATGCTGGAGCGGCAGCGGGAAGGGATCAGGCTGGCCAAAGATG
CAGGGCGCTACCAAGGCCGCAAGGCAACAGCACGGGCACTGTCTGACAAGGCACTTGAACTGCTGGCCCAGGGCAAGACA
AAGCAGGCGGTAGCTGATGAACTGGAAATAGGTATTGCCAGCGTGTACCGGATAGCCAAGGCAGCAAAAGCCTGA

Upstream 100 bases:

>100_bases
CCGCTGATTTTTGAAAAACCTTGACAATCCTTAACCCTATTGATAGGTTTATCACATAGTATTTAACTTGTTGATAGCAA
CCGCACAGAGAGGAACTATC

Downstream 100 bases:

>100_bases
TTGAATTTTTGTTGTTTATATTTATGCCAATTATGGCATAATGGGGACGCAATGAATAACAAGCCTACAAAACCTCTTGA
GTGGATTGCCAGCAGCCTGA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MAIIGYARVSTEGQELTAQLEQLNGAGVDKIFKEKASGVKQDRPQLAAMLDHLREGDTVIVCKLDRIARSTKHLLEIVES
LEARKVSFKVLNINLDTSTPTGKLMLSMLASIAQFEREMMLERQREGIRLAKDAGRYQGRKATARALSDKALELLAQGKT
KQAVADELEIGIASVYRIAKAAKA

Sequences:

>Translated_184_residues
MAIIGYARVSTEGQELTAQLEQLNGAGVDKIFKEKASGVKQDRPQLAAMLDHLREGDTVIVCKLDRIARSTKHLLEIVES
LEARKVSFKVLNINLDTSTPTGKLMLSMLASIAQFEREMMLERQREGIRLAKDAGRYQGRKATARALSDKALELLAQGKT
KQAVADELEIGIASVYRIAKAAKA
>Mature_183_residues
AIIGYARVSTEGQELTAQLEQLNGAGVDKIFKEKASGVKQDRPQLAAMLDHLREGDTVIVCKLDRIARSTKHLLEIVESL
EARKVSFKVLNINLDTSTPTGKLMLSMLASIAQFEREMMLERQREGIRLAKDAGRYQGRKATARALSDKALELLAQGKTK
QAVADELEIGIASVYRIAKAAKA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=177, Percent_Identity=40.1129943502825, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1787827, Length=187, Percent_Identity=31.5508021390374, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787638, Length=187, Percent_Identity=31.0160427807487, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20223; Mature: 20092

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIGYARVSTEGQELTAQLEQLNGAGVDKIFKEKASGVKQDRPQLAAMLDHLREGDTVI
CEEEECHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEE
VCKLDRIARSTKHLLEIVESLEARKVSFKVLNINLDTSTPTGKLMLSMLASIAQFEREMM
EEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LERQREGIRLAKDAGRYQGRKATARALSDKALELLAQGKTKQAVADELEIGIASVYRIAK
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AAKA
HHCC
>Mature Secondary Structure 
AIIGYARVSTEGQELTAQLEQLNGAGVDKIFKEKASGVKQDRPQLAAMLDHLREGDTVI
EEEECHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEE
VCKLDRIARSTKHLLEIVESLEARKVSFKVLNINLDTSTPTGKLMLSMLASIAQFEREMM
EEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LERQREGIRLAKDAGRYQGRKATARALSDKALELLAQGKTKQAVADELEIGIASVYRIAK
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AAKA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2997130 [H]