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The map label for this gene is glyS

Identifier: 189424088

GI number: 189424088

Start: 1061579

End: 1063642

Strand: Reverse

Name: glyS

Synonym: Glov_1022

Alternate gene names: 189424088

Gene position: 1063642-1061579 (Counterclockwise)

Preceding gene: 189424089

Following gene: 189424087

Centisome position: 27.15

GC content: 59.11

Gene sequence:

>2064_bases
ATGTCCAAGGAACTGTTGCTTGAAATTGGTGCCGAGGAAATCCCGGCCGGATTCGTACCCAAGGCGCTTGCATCCCTTGA
AGAGATGATCCGCAAAGAGCTTGAGACTGCCCGGCTCTGTTTTGATGCCATCGTGACCATGGGAACCCCGCGCCGCCTGA
CGCTGCATATCAAAGGACTGCCAGTCATCCAGCCCGATGCTGAACTGACCGCCATGGGGCCGTCAAAGAAGGCAGCATTT
GATGCCGACGGCAAACCGACCAAGGCTGCTGAAGGGTTTGCCCGTGGCCAGGGGGTGGACGTCTCTGCCCTGCAGGTTAT
CAGCACTGACAAAGGCGAATATCTGGCGGTTACCAGACAGGAAACCGGACGCCCGACCCATGAGCTGCTGGCCGAGATTC
TACCCAGGCTGGTGGCCGGTATCCCGTTCAAGAAATCAATGCGCTGGGCAGATCTGGATATCCGCTTCGCCCGTCCGGTA
CACTGGATTGTGGCCCTGTTCGACGGCATCGTGGTGCCGTTCAGTTTTGGTCCGATCCAGAGCGGCAACATCTCCCGCGG
CCACCGTTTCATGGCCAACAGTACCTTTCCGGTGCGGGACTTTGCCCACTACCTGGATGAGTGCGAACGTCATTTCGTCA
TCGTTGATCAGGAACGTCGCAAAGAGACCATCCGTAAGGAGACCCACCGGGTTGCCAAGACCACCGGTGGACATCTGCTG
CCGGATGAGTCGTTGCTGGAAGAGGTCACCTACCTGGTGGAGTACCCCAGCGCCATTATCGGCAGCATCCCGGCCGAATT
CCTGGTGGTGCCCAAAGAGGTGCTGATCACCTCCATGCGTTCCCACCAGCGCTACTTCTCGGTGGTGGATGAAAACGGCA
AGCTGCTGCCCTACTTCATTACCATCCCCAACACCCTGGCCGAAGATCCGGCGGTGGTGGTCAGGGGCAATGAACGCGTT
TTGCGGGCCCGCCTGTCCGATGCCCGTTTCTTCTTTGACGAAGACCGCAAGCTGCGGCTTGAAAGCCGGGTCGAGTCGCT
CAAAAGTGTCGTCTACCAGCAGAAACTGGGCACCTCCTATGAGAAGATGGAGCGTTTCCGGGCATTGGCTGAACAGCTGG
CTGAGCAGCTCAACCCTGCTGCCAAACAACAGGCCGCACGGACAGCCTTCCTCTGCAAGGCCGATCTGGTCTCCGGCATG
GTGGGCGAATTCCCCGAGGTGCAGGGGATTATGGGACGCGAGTACGCCCTGCATGATGGTGAAGAGACGGCCGTGGCCAA
TGCGATTGCCGAGCATTACCTGCCCACCCAGGCCGGTGGTGACCTGCCCGCTTCGGATATCGGCGCTTTTGTCTCGCTGG
CTGACAAACTGGATACCCTCTGCGGCTGCTTTGGTGTCGGGCTGATACCGACCGGTGCTGCCGACCCCTATGCCCTGCGC
CGTGCCACCATCGGCATGATCAGCATCATTCTGGACAAGGGCTACAGCCTGTCATTATCCGGACTGATCGACAAATCACT
TGAGTTGCTGGCCGCAAAACTGACCCGTCCCCGTGACGAGGTCCGTCACGATGTGCTGGAGTTTTTCCGGGGCCGTTTTG
TCAACCTGCAGGGTAATGCCTATCCGGCCGACGTGGTTGAGGCGGCAATGGCTGCCGGGTTTGATGATCTGGCCGACTGC
GCCGAACGGATCCGCGCCCTGGACACCTTCCGCCAGCGGGATGATTTCCAGCCGCTGACCGTGGCCTTCAAACGGGTCTG
CAACATCATCAAGGAGGGGATCGATGCCCCGGTTGCCCCGGCCCTGTTTCAGGATGAAGCGGAGCACACCCTTTACCGGG
TGCTGCAGGAAACCAAGCTCTCCGCCTCAGACAAGATTCAGCAGCAGCAGTACCTGGAGGCACTGACCGACATAGCCGGT
CTGAAATGGGCGGTGGATGCCTTCTTCGATGCAGTCATGGTCATGGCGGAGGATAGTGCCGTCCGCAACAACCGTCTGGC
ACTTTTGACCACCATCAACCGACTTTTCAGCAGGATTGCAGACTTTGGCAGATTGGCTGGCTAA

Upstream 100 bases:

>100_bases
CATCGGCCGGGTACGCAACGTGGCCCGCCTCTGCGCTGAAGGCTATGTCCGGCAGCGCGAACGGCTGGGCTATCCGATGC
TGAAAGGGGGGAACTAGACC

Downstream 100 bases:

>100_bases
ACACGTAACTGTTTAATTAAAACAGGAGGAGCAGCATGTCATTCACCGGTCGCATAGTCTTGGGTAACGCCATTGTTGTT
GTCATTGCCGTACTTGCTCT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSKELLLEIGAEEIPAGFVPKALASLEEMIRKELETARLCFDAIVTMGTPRRLTLHIKGLPVIQPDAELTAMGPSKKAAF
DADGKPTKAAEGFARGQGVDVSALQVISTDKGEYLAVTRQETGRPTHELLAEILPRLVAGIPFKKSMRWADLDIRFARPV
HWIVALFDGIVVPFSFGPIQSGNISRGHRFMANSTFPVRDFAHYLDECERHFVIVDQERRKETIRKETHRVAKTTGGHLL
PDESLLEEVTYLVEYPSAIIGSIPAEFLVVPKEVLITSMRSHQRYFSVVDENGKLLPYFITIPNTLAEDPAVVVRGNERV
LRARLSDARFFFDEDRKLRLESRVESLKSVVYQQKLGTSYEKMERFRALAEQLAEQLNPAAKQQAARTAFLCKADLVSGM
VGEFPEVQGIMGREYALHDGEETAVANAIAEHYLPTQAGGDLPASDIGAFVSLADKLDTLCGCFGVGLIPTGAADPYALR
RATIGMISIILDKGYSLSLSGLIDKSLELLAAKLTRPRDEVRHDVLEFFRGRFVNLQGNAYPADVVEAAMAAGFDDLADC
AERIRALDTFRQRDDFQPLTVAFKRVCNIIKEGIDAPVAPALFQDEAEHTLYRVLQETKLSASDKIQQQQYLEALTDIAG
LKWAVDAFFDAVMVMAEDSAVRNNRLALLTTINRLFSRIADFGRLAG

Sequences:

>Translated_687_residues
MSKELLLEIGAEEIPAGFVPKALASLEEMIRKELETARLCFDAIVTMGTPRRLTLHIKGLPVIQPDAELTAMGPSKKAAF
DADGKPTKAAEGFARGQGVDVSALQVISTDKGEYLAVTRQETGRPTHELLAEILPRLVAGIPFKKSMRWADLDIRFARPV
HWIVALFDGIVVPFSFGPIQSGNISRGHRFMANSTFPVRDFAHYLDECERHFVIVDQERRKETIRKETHRVAKTTGGHLL
PDESLLEEVTYLVEYPSAIIGSIPAEFLVVPKEVLITSMRSHQRYFSVVDENGKLLPYFITIPNTLAEDPAVVVRGNERV
LRARLSDARFFFDEDRKLRLESRVESLKSVVYQQKLGTSYEKMERFRALAEQLAEQLNPAAKQQAARTAFLCKADLVSGM
VGEFPEVQGIMGREYALHDGEETAVANAIAEHYLPTQAGGDLPASDIGAFVSLADKLDTLCGCFGVGLIPTGAADPYALR
RATIGMISIILDKGYSLSLSGLIDKSLELLAAKLTRPRDEVRHDVLEFFRGRFVNLQGNAYPADVVEAAMAAGFDDLADC
AERIRALDTFRQRDDFQPLTVAFKRVCNIIKEGIDAPVAPALFQDEAEHTLYRVLQETKLSASDKIQQQQYLEALTDIAG
LKWAVDAFFDAVMVMAEDSAVRNNRLALLTTINRLFSRIADFGRLAG
>Mature_686_residues
SKELLLEIGAEEIPAGFVPKALASLEEMIRKELETARLCFDAIVTMGTPRRLTLHIKGLPVIQPDAELTAMGPSKKAAFD
ADGKPTKAAEGFARGQGVDVSALQVISTDKGEYLAVTRQETGRPTHELLAEILPRLVAGIPFKKSMRWADLDIRFARPVH
WIVALFDGIVVPFSFGPIQSGNISRGHRFMANSTFPVRDFAHYLDECERHFVIVDQERRKETIRKETHRVAKTTGGHLLP
DESLLEEVTYLVEYPSAIIGSIPAEFLVVPKEVLITSMRSHQRYFSVVDENGKLLPYFITIPNTLAEDPAVVVRGNERVL
RARLSDARFFFDEDRKLRLESRVESLKSVVYQQKLGTSYEKMERFRALAEQLAEQLNPAAKQQAARTAFLCKADLVSGMV
GEFPEVQGIMGREYALHDGEETAVANAIAEHYLPTQAGGDLPASDIGAFVSLADKLDTLCGCFGVGLIPTGAADPYALRR
ATIGMISIILDKGYSLSLSGLIDKSLELLAAKLTRPRDEVRHDVLEFFRGRFVNLQGNAYPADVVEAAMAAGFDDLADCA
ERIRALDTFRQRDDFQPLTVAFKRVCNIIKEGIDAPVAPALFQDEAEHTLYRVLQETKLSASDKIQQQQYLEALTDIAGL
KWAVDAFFDAVMVMAEDSAVRNNRLALLTTINRLFSRIADFGRLAG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=698, Percent_Identity=40.4011461318052, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_GEOLS (B3E621)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951265.1
- ProteinModelPortal:   B3E621
- GeneID:   6369067
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_1022
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76129; Mature: 75998

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLEIGAEEIPAGFVPKALASLEEMIRKELETARLCFDAIVTMGTPRRLTLHIKGL
CCCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
PVIQPDAELTAMGPSKKAAFDADGKPTKAAEGFARGQGVDVSALQVISTDKGEYLAVTRQ
EEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEEECCCCCEEEEECC
ETGRPTHELLAEILPRLVAGIPFKKSMRWADLDIRFARPVHWIVALFDGIVVPFSFGPIQ
CCCCCHHHHHHHHHHHHHHCCCCHHCCCEECCCEEHHHHHHHHHHHHCCCEECCCCCCCC
SGNISRGHRFMANSTFPVRDFAHYLDECERHFVIVDQERRKETIRKETHRVAKTTGGHLL
CCCCCCCCEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCC
PDESLLEEVTYLVEYPSAIIGSIPAEFLVVPKEVLITSMRSHQRYFSVVDENGKLLPYFI
CCHHHHHHHHHHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
TIPNTLAEDPAVVVRGNERVLRARLSDARFFFDEDRKLRLESRVESLKSVVYQQKLGTSY
ECCCHHCCCCEEEEECCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCH
EKMERFRALAEQLAEQLNPAAKQQAARTAFLCKADLVSGMVGEFPEVQGIMGREYALHDG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
EETAVANAIAEHYLPTQAGGDLPASDIGAFVSLADKLDTLCGCFGVGLIPTGAADPYALR
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RATIGMISIILDKGYSLSLSGLIDKSLELLAAKLTRPRDEVRHDVLEFFRGRFVNLQGNA
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCC
YPADVVEAAMAAGFDDLADCAERIRALDTFRQRDDFQPLTVAFKRVCNIIKEGIDAPVAP
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
ALFQDEAEHTLYRVLQETKLSASDKIQQQQYLEALTDIAGLKWAVDAFFDAVMVMAEDSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VRNNRLALLTTINRLFSRIADFGRLAG
HCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKELLLEIGAEEIPAGFVPKALASLEEMIRKELETARLCFDAIVTMGTPRRLTLHIKGL
CCHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
PVIQPDAELTAMGPSKKAAFDADGKPTKAAEGFARGQGVDVSALQVISTDKGEYLAVTRQ
EEECCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHEEECCCCCEEEEECC
ETGRPTHELLAEILPRLVAGIPFKKSMRWADLDIRFARPVHWIVALFDGIVVPFSFGPIQ
CCCCCHHHHHHHHHHHHHHCCCCHHCCCEECCCEEHHHHHHHHHHHHCCCEECCCCCCCC
SGNISRGHRFMANSTFPVRDFAHYLDECERHFVIVDQERRKETIRKETHRVAKTTGGHLL
CCCCCCCCEECCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCC
PDESLLEEVTYLVEYPSAIIGSIPAEFLVVPKEVLITSMRSHQRYFSVVDENGKLLPYFI
CCHHHHHHHHHHHHHHHHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
TIPNTLAEDPAVVVRGNERVLRARLSDARFFFDEDRKLRLESRVESLKSVVYQQKLGTSY
ECCCHHCCCCEEEEECCHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCH
EKMERFRALAEQLAEQLNPAAKQQAARTAFLCKADLVSGMVGEFPEVQGIMGREYALHDG
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
EETAVANAIAEHYLPTQAGGDLPASDIGAFVSLADKLDTLCGCFGVGLIPTGAADPYALR
CHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RATIGMISIILDKGYSLSLSGLIDKSLELLAAKLTRPRDEVRHDVLEFFRGRFVNLQGNA
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCC
YPADVVEAAMAAGFDDLADCAERIRALDTFRQRDDFQPLTVAFKRVCNIIKEGIDAPVAP
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
ALFQDEAEHTLYRVLQETKLSASDKIQQQQYLEALTDIAGLKWAVDAFFDAVMVMAEDSA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VRNNRLALLTTINRLFSRIADFGRLAG
HCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA