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The map label for this gene is rsmE [H]

Identifier: 189424061

GI number: 189424061

Start: 1033551

End: 1034300

Strand: Reverse

Name: rsmE [H]

Synonym: Glov_0995

Alternate gene names: 189424061

Gene position: 1034300-1033551 (Counterclockwise)

Preceding gene: 189424062

Following gene: 189424060

Centisome position: 26.4

GC content: 60.93

Gene sequence:

>750_bases
ATGAGCGGACGACGCCGCTTTATTGTCAGCTCCCGCAGCATCCGCGATGGCTACGCCTCCTATGACGGCCCCCAGCATCA
CCACATGGCACGGGTGCTGCGGCTCAGATCAGGCGACGAGGTAATGCTGATTGATGAACAGGGCCGGCGTCATCTGGGAG
TGATCGATCAGGTTACCCAGGAGTGGACCGCGGTACGCCTGACCGAGACCCTTGAGGCATCCCAGTTTCCCGATGCCCCG
GCCATCACGGTGATTCAGGGGCTGCCCAAGGGAGAAAAGATCGAGCTGGTGCTGCAAAAAGGGACCGAACTGGGGGTAAG
CGAATTCTCCATCTTCAGGGCGGATCGTTCAGTGCCTAAACTGGCCGGTGACAAGCTGCAGGGCCGGATTGAGCGCTGGA
ACCGGATTGTGGCCGAGGCAGCCCGCCAATCAGAACGTTTTGATCAGCCAACGGTTGCCTGGCATCCCTCAGCGGCAGCA
GCAGCAGCCGGTGCTGCCAACGCAGAACTGAAACTGCTGCTCTGGGAACGGGGGGCACCGACCCCGCTGCGGGAGGTTCT
GGAGGGGATGCCCCGTCCGGCACGGATTGCGATTGCCATCGGCCCTGAAGGGGGCTTTGAACCGGGCGAGGCAGAGGCCT
TTTCATTCTGGGGCTTTACACCGGTCACCCTGGGAGAACGGATTCTGCGCACCGAAACAGCTGCCCTTGCCATGACTGCA
ATTGTGCAGTATAAGTGGGGAGATATCTAG

Upstream 100 bases:

>100_bases
GGGAAGCGCTGGTCCGCAACGGCTTTGATCCGTTTCCGCTCAGCTTCGAGGCTTCTCTGGCAGCAGGAGAATGGCGCTGC
CTGCACTACCGGAGGCTGCC

Downstream 100 bases:

>100_bases
AGCTGCACCAAGGAGGGTACACCAGATGAGATGTCCTAAATGCGGTTATACCAGCTTTGAAAGCTACGATTCCTGTCGCA
AATGTTCTGCCGACCTGGTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSGRRRFIVSSRSIRDGYASYDGPQHHHMARVLRLRSGDEVMLIDEQGRRHLGVIDQVTQEWTAVRLTETLEASQFPDAP
AITVIQGLPKGEKIELVLQKGTELGVSEFSIFRADRSVPKLAGDKLQGRIERWNRIVAEAARQSERFDQPTVAWHPSAAA
AAAGAANAELKLLLWERGAPTPLREVLEGMPRPARIAIAIGPEGGFEPGEAEAFSFWGFTPVTLGERILRTETAALAMTA
IVQYKWGDI

Sequences:

>Translated_249_residues
MSGRRRFIVSSRSIRDGYASYDGPQHHHMARVLRLRSGDEVMLIDEQGRRHLGVIDQVTQEWTAVRLTETLEASQFPDAP
AITVIQGLPKGEKIELVLQKGTELGVSEFSIFRADRSVPKLAGDKLQGRIERWNRIVAEAARQSERFDQPTVAWHPSAAA
AAAGAANAELKLLLWERGAPTPLREVLEGMPRPARIAIAIGPEGGFEPGEAEAFSFWGFTPVTLGERILRTETAALAMTA
IVQYKWGDI
>Mature_248_residues
SGRRRFIVSSRSIRDGYASYDGPQHHHMARVLRLRSGDEVMLIDEQGRRHLGVIDQVTQEWTAVRLTETLEASQFPDAPA
ITVIQGLPKGEKIELVLQKGTELGVSEFSIFRADRSVPKLAGDKLQGRIERWNRIVAEAARQSERFDQPTVAWHPSAAAA
AAGAANAELKLLLWERGAPTPLREVLEGMPRPARIAIAIGPEGGFEPGEAEAFSFWGFTPVTLGERILRTETAALAMTAI
VQYKWGDI

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=228, Percent_Identity=30.7017543859649, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27505; Mature: 27373

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRRFIVSSRSIRDGYASYDGPQHHHMARVLRLRSGDEVMLIDEQGRRHLGVIDQVTQ
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
EWTAVRLTETLEASQFPDAPAITVIQGLPKGEKIELVLQKGTELGVSEFSIFRADRSVPK
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCHHHHEECCCCCCCH
LAGDKLQGRIERWNRIVAEAARQSERFDQPTVAWHPSAAAAAAGAANAELKLLLWERGAP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCEEEEEEECCCCC
TPLREVLEGMPRPARIAIAIGPEGGFEPGEAEAFSFWGFTPVTLGERILRTETAALAMTA
CHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IVQYKWGDI
HHHHCCCCC
>Mature Secondary Structure 
SGRRRFIVSSRSIRDGYASYDGPQHHHMARVLRLRSGDEVMLIDEQGRRHLGVIDQVTQ
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHH
EWTAVRLTETLEASQFPDAPAITVIQGLPKGEKIELVLQKGTELGVSEFSIFRADRSVPK
HHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCHHHHEECCCCCCCH
LAGDKLQGRIERWNRIVAEAARQSERFDQPTVAWHPSAAAAAAGAANAELKLLLWERGAP
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCEEEEEEECCCCC
TPLREVLEGMPRPARIAIAIGPEGGFEPGEAEAFSFWGFTPVTLGERILRTETAALAMTA
CHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
IVQYKWGDI
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]