Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 189424029

GI number: 189424029

Start: 1002766

End: 1003668

Strand: Reverse

Name: pleD [H]

Synonym: Glov_0963

Alternate gene names: 189424029

Gene position: 1003668-1002766 (Counterclockwise)

Preceding gene: 189424030

Following gene: 189424028

Centisome position: 25.62

GC content: 53.27

Gene sequence:

>903_bases
ATGAGCATTCTAGTCGTTGAAGACAACCGCGAAACCCGTCGTAAAATAGTGGCTGACCTTAAGCAAAACGGATTTAAGGA
TGCCGTGTTCGAGGCAGTAGACGGCCTGGATGGTTTTCAAAAGGCCTCGCAGAACGCCCCCGACGTCATCCTGTGTGATA
TTGAAATGCCACGCATGGACGGCCTTAAGTTTTTGACTGCAATCCGCTCAAGAACCGAGACAGAGCACATTCCGGTACTG
CTGCTGACCAGCAATACCAGTCGCGACATCCGACTGAAGGGCCTGACAACAGGAGCGCACGATTTTATTCATATCCCCTA
TGATCCGCAGGAACTTGTAGCTCGGGCAAAACTGCACCTGAACGCAAAACGTCGTGAAGAGGAGCTCCGCAAACGGAACA
AGGAACTTGAACTGCTTTCACATAAGGATGCGCTGACCGGTGCCTTTAACCGGCGCTACCTCTGCCACATCCTCAATGTT
GAGCTGGGCCGGGCGGCACGCACCGACGGCATGGTGTCGGTGCTGATGCTTGATATTGACCACTTTAAAGAAATCAACGA
CAGATTTGGTCATCAGGCAGGTGATTTGGCGCTCAAAAAAGTGACGGCAGAGGCCACGGCCTGCTTACGGGACTACGACC
TCTTTTTCCGATATGGCGGTGAGGAGTTTGTGGTGATGCTGCCCGACACCAGCAAGCGTGAGGCCTTAAAGGTGGCCCAT
CGGCTGTGCCAAAAAGTTCGCGCAATTGCTTTTTCCCACATCTCCCCCGACCTGAAGATAACGGTCAGCATCGGTGTTGC
AGCATATCCGGACAATGACGTCTGCTCCGTTGAGGAACTGCTTGCACATGCCGATCAAGCGCTGTATCAGGCAAAACAGA
GCGGCAGGGACCAGGTGGCGTAA

Upstream 100 bases:

>100_bases
ACAGCAGAACACCGGGCAAAAATGAACGTTATGCCCAACGCATTGCAGCACAATTGGCAAACCACAGCATGCAGGCATCA
CTGACCCAGGATGGTGCACG

Downstream 100 bases:

>100_bases
TTTCTGCTTTACTGAACTGCCAGACAGCAAAAGTCCTGAAACCCAAATATGTAACGTCTTGACCGCCCGGCTGTCATATA
AAACGGGGGTATCCCCAATA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSILVVEDNRETRRKIVADLKQNGFKDAVFEAVDGLDGFQKASQNAPDVILCDIEMPRMDGLKFLTAIRSRTETEHIPVL
LLTSNTSRDIRLKGLTTGAHDFIHIPYDPQELVARAKLHLNAKRREEELRKRNKELELLSHKDALTGAFNRRYLCHILNV
ELGRAARTDGMVSVLMLDIDHFKEINDRFGHQAGDLALKKVTAEATACLRDYDLFFRYGGEEFVVMLPDTSKREALKVAH
RLCQKVRAIAFSHISPDLKITVSIGVAAYPDNDVCSVEELLAHADQALYQAKQSGRDQVA

Sequences:

>Translated_300_residues
MSILVVEDNRETRRKIVADLKQNGFKDAVFEAVDGLDGFQKASQNAPDVILCDIEMPRMDGLKFLTAIRSRTETEHIPVL
LLTSNTSRDIRLKGLTTGAHDFIHIPYDPQELVARAKLHLNAKRREEELRKRNKELELLSHKDALTGAFNRRYLCHILNV
ELGRAARTDGMVSVLMLDIDHFKEINDRFGHQAGDLALKKVTAEATACLRDYDLFFRYGGEEFVVMLPDTSKREALKVAH
RLCQKVRAIAFSHISPDLKITVSIGVAAYPDNDVCSVEELLAHADQALYQAKQSGRDQVA
>Mature_299_residues
SILVVEDNRETRRKIVADLKQNGFKDAVFEAVDGLDGFQKASQNAPDVILCDIEMPRMDGLKFLTAIRSRTETEHIPVLL
LTSNTSRDIRLKGLTTGAHDFIHIPYDPQELVARAKLHLNAKRREEELRKRNKELELLSHKDALTGAFNRRYLCHILNVE
LGRAARTDGMVSVLMLDIDHFKEINDRFGHQAGDLALKKVTAEATACLRDYDLFFRYGGEEFVVMLPDTSKREALKVAHR
LCQKVRAIAFSHISPDLKITVSIGVAAYPDNDVCSVEELLAHADQALYQAKQSGRDQVA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=40.5063291139241, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=38.953488372093, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=34.3023255813954, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=34.7560975609756, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=36.7469879518072, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082007, Length=131, Percent_Identity=44.2748091603053, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081974, Length=152, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=39.1304347826087, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=31.3953488372093, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=37.1900826446281, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=30.952380952381, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788394, Length=152, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=34.1880341880342, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=32.7586206896552, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790299, Length=170, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787056, Length=127, Percent_Identity=33.8582677165354, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=31.0924369747899, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790863, Length=115, Percent_Identity=32.1739130434783, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33806; Mature: 33675

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILVVEDNRETRRKIVADLKQNGFKDAVFEAVDGLDGFQKASQNAPDVILCDIEMPRMD
CEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCCCC
GLKFLTAIRSRTETEHIPVLLLTSNTSRDIRLKGLTTGAHDFIHIPYDPQELVARAKLHL
HHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHC
NAKRREEELRKRNKELELLSHKDALTGAFNRRYLCHILNVELGRAARTDGMVSVLMLDID
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCHHHHEEHHHH
HFKEINDRFGHQAGDLALKKVTAEATACLRDYDLFFRYGGEEFVVMLPDTSKREALKVAH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHH
RLCQKVRAIAFSHISPDLKITVSIGVAAYPDNDVCSVEELLAHADQALYQAKQSGRDQVA
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SILVVEDNRETRRKIVADLKQNGFKDAVFEAVDGLDGFQKASQNAPDVILCDIEMPRMD
EEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCCCCEEEEECCCCCCC
GLKFLTAIRSRTETEHIPVLLLTSNTSRDIRLKGLTTGAHDFIHIPYDPQELVARAKLHL
HHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHC
NAKRREEELRKRNKELELLSHKDALTGAFNRRYLCHILNVELGRAARTDGMVSVLMLDID
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCHHHHEEHHHH
HFKEINDRFGHQAGDLALKKVTAEATACLRDYDLFFRYGGEEFVVMLPDTSKREALKVAH
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHH
RLCQKVRAIAFSHISPDLKITVSIGVAAYPDNDVCSVEELLAHADQALYQAKQSGRDQVA
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA