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The map label for this gene is phsA [H]

Identifier: 189423996

GI number: 189423996

Start: 958836

End: 961031

Strand: Reverse

Name: phsA [H]

Synonym: Glov_0930

Alternate gene names: 189423996

Gene position: 961031-958836 (Counterclockwise)

Preceding gene: 189423997

Following gene: 189423995

Centisome position: 24.53

GC content: 62.39

Gene sequence:

>2196_bases
ATGACCATGCCACAGACAGTAAAGACAAACTGCCGCTTTTGCGGCTATCAATGCGGCCTGACCGCCACCGTGGAAAACGG
CAGAGTGACCGGGGTTGCCCCGGACCCGACCCAGTATCCGGGTGATGAACGTATTCAGCACGGCTGCCATCGCTGGCGGA
TGGCACCGCAGTTTCTGGACCACCCTGACCGCGTCAACTACCCCCTGAAACGGGTTGGTGAACGGGGCAGCGGCCAGTGG
CATCGGATCAGCTGGGACCAGGCCCTGGACGAGATTGCGGAAAAGCTGGCCGGGCTGAAGGAACAGTTCGGGCCGGAAAC
CCTGGCCACCTGCATCGGCGGCCCCCATGCCGTCTACTGGCCCCTGCACCGTTTCATGACCCTCTTCGGCAGTCCCAACA
ATGTCGGTATCGGCCAGATCTGCTGGAACCCCGGCACCCTGCTGCATGCGATCAGCTACGGCTGGCCCCTCGATAACGAG
CTGGAACCTGACCTGACCGGCTGCCTGATGTTGTGGGGGGCAAACCACGCCGAATCCGACAACTCACTGCTGTGGCACAG
GATCCAGCAGTACAGCCGGACCGGCAAACCGCTGATCGTGGTCGATCCCCGCCGCACCAGGACGGCTGAGCTGGCAACCC
ACTGGCTCCCGGTCCGGCCCGGTACCGATGCGGTGCTCGCCCTGGGACTCGTCCAGGCCATCATCCAGAACCGCTGGTAC
GACGCCCCCTTTGTGGAGGCCTGGTGCCACGGCTTTGCCAGGCTGGCCGGACACCTGCAACACTACACTCCCGGCTACGT
TGCAGAGGCCACCGGCATACCAGCGGAGCAGCTACTGGAGGTAGCCCGTCTGTACGCCCTGCAGTGTCCCTCTGCGCTGT
TTACCGGACGGGGTATCGACCAGATCGGCCGGAACAGCGTGCCGCTTCATCACTCCCTTGATATTCTGCGGGCCATCACC
GGCAATATTGACCGCAAAGGGGCCTCCTGCATCACCGGGATGCCTGACTTCATACCAGAGCTTGAGTTGGAGCTGAGTGA
CCGGTTTTTGGAGACACAACGCAGGAAGCAGCTCGGCCAGCTGAAGCTGCAGAGCTATCACGGCTACGAACAGATCAGAA
AGCTGACCAGCAAACAGGACAAACGGTTGCCGATGCGCTACCTGACCTCGACGCAACCCAACCTGGTCTGGCAGGCGATG
CTGACCGACCGACCCTACCCGATCCGCTCCATGATTGTCATGGCCACCAACCCGCTCTTGACCCAGGCGGATACCAGCCT
GGTCTACCAGGCACTCAAGAGCCTGGATCTGCTGGTGGTGCTGGAACTGTTCCAGACCCCGACAGCCATGCTGGCCGACT
ACCTGCTACCCAGTGCCGGTGTGCTGGAGCGGGCCCTGTTCGAGACCAAGGCCGGCACCGCCAACCTGGCCTACGGCGGC
GCCAAGGCGGTGGAACCGTACTACGAACGGCGGCCTGACTTTGATTTCTGGCAGGGACTGGGGCGCCGGCTGGGTCAGCA
GGAAGACTGGCCCTGGCAGAGCTTCGAGGACAGCCTTGAGGCCACCCTTGCCCCAACCGGGGTTGACTGGGAGACCTTCA
GCCGTCATGGCCTGTACACGCTGCCGGAGGAGTACGAAAAACACGAAAGAATCGATCCGGCCACCGGCAAGGCCGCCGGA
TTTGCCACCACAACCGGCAAGATTGAGCTGTACAGCGAGCTGCTTGCCGGACTCGGGGCAGAACCGTTGCCGGTCCCCAA
ACCGGTGGCGGCTGTTGACGAGCAGTATCCGCTTTCCCTGATCACCGGGGCCAGGTATCACCCCTACTACGCCTCCAGCT
TTCATCAGCTTGAGGGGTTGCGCAAGCTGCATCCCGAGCCCTGGGCCGAGGTGAGCCGGGCAACTGCCCACAAGCTCGGC
CTGGCAGAAGGTACACCCGTCCGGGTTACCACCGAAAAGGGAACTGCCTGCTTTACACTCAAGATCTCCGAGATGTGCGA
TGACGTGGTCAGTGTCGAGTATGGCTGGTGGTACCCTGAGAAGAAGGCCGCCGAGCCGGAACTGGGCGGACTGTGGCTGG
CCAACGCCAATCTGCTGACCAGCGCCGACTATGCAACGGCAGATCCGCTGATCGGTACCGCCACCTACAACGGCATTCCC
TGTTGCCTGCAGGCTGTTGACAGTGGTAAGAAATGA

Upstream 100 bases:

>100_bases
AGCATGACGGTATCGCGGGCTGATGGCTGCATCGTCCCGTTCTGTTCCTATCAATTGACCTCCCTCGACGGCACGAAACG
AAAGACCCGGTAATCTGATT

Downstream 100 bases:

>100_bases
GCCCGGGAAAAACAGTTCCCGCGGAGGCAAAAGATGATGATTGAACCGCAGCAACTGATCCTGTTCTTTACCACCTCGGT
CCTGCTGGGCCTGGCGCCGG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MTMPQTVKTNCRFCGYQCGLTATVENGRVTGVAPDPTQYPGDERIQHGCHRWRMAPQFLDHPDRVNYPLKRVGERGSGQW
HRISWDQALDEIAEKLAGLKEQFGPETLATCIGGPHAVYWPLHRFMTLFGSPNNVGIGQICWNPGTLLHAISYGWPLDNE
LEPDLTGCLMLWGANHAESDNSLLWHRIQQYSRTGKPLIVVDPRRTRTAELATHWLPVRPGTDAVLALGLVQAIIQNRWY
DAPFVEAWCHGFARLAGHLQHYTPGYVAEATGIPAEQLLEVARLYALQCPSALFTGRGIDQIGRNSVPLHHSLDILRAIT
GNIDRKGASCITGMPDFIPELELELSDRFLETQRRKQLGQLKLQSYHGYEQIRKLTSKQDKRLPMRYLTSTQPNLVWQAM
LTDRPYPIRSMIVMATNPLLTQADTSLVYQALKSLDLLVVLELFQTPTAMLADYLLPSAGVLERALFETKAGTANLAYGG
AKAVEPYYERRPDFDFWQGLGRRLGQQEDWPWQSFEDSLEATLAPTGVDWETFSRHGLYTLPEEYEKHERIDPATGKAAG
FATTTGKIELYSELLAGLGAEPLPVPKPVAAVDEQYPLSLITGARYHPYYASSFHQLEGLRKLHPEPWAEVSRATAHKLG
LAEGTPVRVTTEKGTACFTLKISEMCDDVVSVEYGWWYPEKKAAEPELGGLWLANANLLTSADYATADPLIGTATYNGIP
CCLQAVDSGKK

Sequences:

>Translated_731_residues
MTMPQTVKTNCRFCGYQCGLTATVENGRVTGVAPDPTQYPGDERIQHGCHRWRMAPQFLDHPDRVNYPLKRVGERGSGQW
HRISWDQALDEIAEKLAGLKEQFGPETLATCIGGPHAVYWPLHRFMTLFGSPNNVGIGQICWNPGTLLHAISYGWPLDNE
LEPDLTGCLMLWGANHAESDNSLLWHRIQQYSRTGKPLIVVDPRRTRTAELATHWLPVRPGTDAVLALGLVQAIIQNRWY
DAPFVEAWCHGFARLAGHLQHYTPGYVAEATGIPAEQLLEVARLYALQCPSALFTGRGIDQIGRNSVPLHHSLDILRAIT
GNIDRKGASCITGMPDFIPELELELSDRFLETQRRKQLGQLKLQSYHGYEQIRKLTSKQDKRLPMRYLTSTQPNLVWQAM
LTDRPYPIRSMIVMATNPLLTQADTSLVYQALKSLDLLVVLELFQTPTAMLADYLLPSAGVLERALFETKAGTANLAYGG
AKAVEPYYERRPDFDFWQGLGRRLGQQEDWPWQSFEDSLEATLAPTGVDWETFSRHGLYTLPEEYEKHERIDPATGKAAG
FATTTGKIELYSELLAGLGAEPLPVPKPVAAVDEQYPLSLITGARYHPYYASSFHQLEGLRKLHPEPWAEVSRATAHKLG
LAEGTPVRVTTEKGTACFTLKISEMCDDVVSVEYGWWYPEKKAAEPELGGLWLANANLLTSADYATADPLIGTATYNGIP
CCLQAVDSGKK
>Mature_730_residues
TMPQTVKTNCRFCGYQCGLTATVENGRVTGVAPDPTQYPGDERIQHGCHRWRMAPQFLDHPDRVNYPLKRVGERGSGQWH
RISWDQALDEIAEKLAGLKEQFGPETLATCIGGPHAVYWPLHRFMTLFGSPNNVGIGQICWNPGTLLHAISYGWPLDNEL
EPDLTGCLMLWGANHAESDNSLLWHRIQQYSRTGKPLIVVDPRRTRTAELATHWLPVRPGTDAVLALGLVQAIIQNRWYD
APFVEAWCHGFARLAGHLQHYTPGYVAEATGIPAEQLLEVARLYALQCPSALFTGRGIDQIGRNSVPLHHSLDILRAITG
NIDRKGASCITGMPDFIPELELELSDRFLETQRRKQLGQLKLQSYHGYEQIRKLTSKQDKRLPMRYLTSTQPNLVWQAML
TDRPYPIRSMIVMATNPLLTQADTSLVYQALKSLDLLVVLELFQTPTAMLADYLLPSAGVLERALFETKAGTANLAYGGA
KAVEPYYERRPDFDFWQGLGRRLGQQEDWPWQSFEDSLEATLAPTGVDWETFSRHGLYTLPEEYEKHERIDPATGKAAGF
ATTTGKIELYSELLAGLGAEPLPVPKPVAAVDEQYPLSLITGARYHPYYASSFHQLEGLRKLHPEPWAEVSRATAHKLGL
AEGTPVRVTTEKGTACFTLKISEMCDDVVSVEYGWWYPEKKAAEPELGGLWLANANLLTSADYATADPLIGTATYNGIPC
CLQAVDSGKK

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=753, Percent_Identity=25.3652058432935, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI171474008, Length=740, Percent_Identity=25.4054054054054, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1787870, Length=747, Percent_Identity=25.9705488621151, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI3868721, Length=705, Percent_Identity=24.822695035461, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI145693196, Length=695, Percent_Identity=23.5971223021583, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787231, Length=770, Percent_Identity=22.987012987013, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI87081994, Length=713, Percent_Identity=23.7026647966339, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788534, Length=576, Percent_Identity=22.9166666666667, Blast_Score=93, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 81562; Mature: 81430

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPQTVKTNCRFCGYQCGLTATVENGRVTGVAPDPTQYPGDERIQHGCHRWRMAPQFLD
CCCCCCHHCCCCEECEECCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHCC
HPDRVNYPLKRVGERGSGQWHRISWDQALDEIAEKLAGLKEQFGPETLATCIGGPHAVYW
CCCCCCCCHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHH
PLHRFMTLFGSPNNVGIGQICWNPGTLLHAISYGWPLDNELEPDLTGCLMLWGANHAESD
HHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCHHHEEHEECCCCCCCC
NSLLWHRIQQYSRTGKPLIVVDPRRTRTAELATHWLPVRPGTDAVLALGLVQAIIQNRWY
CHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCC
DAPFVEAWCHGFARLAGHLQHYTPGYVAEATGIPAEQLLEVARLYALQCPSALFTGRGID
CCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHCCCCHH
QIGRNSVPLHHSLDILRAITGNIDRKGASCITGMPDFIPELELELSDRFLETQRRKQLGQ
HHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKLQSYHGYEQIRKLTSKQDKRLPMRYLTSTQPNLVWQAMLTDRPYPIRSMIVMATNPLL
HHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEHHCCCCCCHHHEEEEECCCCH
TQADTSLVYQALKSLDLLVVLELFQTPTAMLADYLLPSAGVLERALFETKAGTANLAYGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCC
AKAVEPYYERRPDFDFWQGLGRRLGQQEDWPWQSFEDSLEATLAPTGVDWETFSRHGLYT
CHHCCHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LPEEYEKHERIDPATGKAAGFATTTGKIELYSELLAGLGAEPLPVPKPVAAVDEQYPLSL
CCHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHH
ITGARYHPYYASSFHQLEGLRKLHPEPWAEVSRATAHKLGLAEGTPVRVTTEKGTACFTL
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEE
KISEMCDDVVSVEYGWWYPEKKAAEPELGGLWLANANLLTSADYATADPLIGTATYNGIP
EHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCEEECCCCCHH
CCLQAVDSGKK
HHHHHHCCCCC
>Mature Secondary Structure 
TMPQTVKTNCRFCGYQCGLTATVENGRVTGVAPDPTQYPGDERIQHGCHRWRMAPQFLD
CCCCCHHCCCCEECEECCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHCC
HPDRVNYPLKRVGERGSGQWHRISWDQALDEIAEKLAGLKEQFGPETLATCIGGPHAVYW
CCCCCCCCHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHH
PLHRFMTLFGSPNNVGIGQICWNPGTLLHAISYGWPLDNELEPDLTGCLMLWGANHAESD
HHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCHHHEEHEECCCCCCCC
NSLLWHRIQQYSRTGKPLIVVDPRRTRTAELATHWLPVRPGTDAVLALGLVQAIIQNRWY
CHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCC
DAPFVEAWCHGFARLAGHLQHYTPGYVAEATGIPAEQLLEVARLYALQCPSALFTGRGID
CCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHCCHHHHCCCCHH
QIGRNSVPLHHSLDILRAITGNIDRKGASCITGMPDFIPELELELSDRFLETQRRKQLGQ
HHCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKLQSYHGYEQIRKLTSKQDKRLPMRYLTSTQPNLVWQAMLTDRPYPIRSMIVMATNPLL
HHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEHHCCCCCCHHHEEEEECCCCH
TQADTSLVYQALKSLDLLVVLELFQTPTAMLADYLLPSAGVLERALFETKAGTANLAYGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEECCC
AKAVEPYYERRPDFDFWQGLGRRLGQQEDWPWQSFEDSLEATLAPTGVDWETFSRHGLYT
CHHCCHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCC
LPEEYEKHERIDPATGKAAGFATTTGKIELYSELLAGLGAEPLPVPKPVAAVDEQYPLSL
CCHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHH
ITGARYHPYYASSFHQLEGLRKLHPEPWAEVSRATAHKLGLAEGTPVRVTTEKGTACFTL
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEE
KISEMCDDVVSVEYGWWYPEKKAAEPELGGLWLANANLLTSADYATADPLIGTATYNGIP
EHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCEEECCCCCHH
CCLQAVDSGKK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]