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The map label for this gene is mraW

Identifier: 189423747

GI number: 189423747

Start: 687002

End: 687943

Strand: Reverse

Name: mraW

Synonym: Glov_0678

Alternate gene names: 189423747

Gene position: 687943-687002 (Counterclockwise)

Preceding gene: 189423748

Following gene: 189423746

Centisome position: 17.56

GC content: 58.49

Gene sequence:

>942_bases
ATGGAGGAATTTCACCACCTGTCAGTGCTGGCCCGGGAGGTCATGGAGCAACTGGCACCCCGCCCCGGCGGCACCTACCT
GGACGGCACCCTGGGCGGCGGCGGGCATAGTGAACTGATTCTTGAAAAGATCGGTCCGGATGGCCTGTTGATCGGTATCG
ACCGCGACCCGGCGGCCCTGGCTGCAGCATCTGAACGGCTGCGACGCTTTGGGAGCTGTTTCAGGCCGCTTCAAGGCAGC
TTTGGGGATCTGGCTGAGTTACTGAAACAAGAAGGGATCAACACCCTTGACGGGTTACTGCTCGATCTCGGCGTTTCGTC
GCACCAACTGGATACCGATGAGCGCGGCTTCAGTTTCCGCCTTGACGGACCACTGGATATGCGAATGGACCGTTCCTGCG
GCGACTCTGCTGCAGACCTGCTGCAGGATTGTTCTGCCGGGGAACTGGAACAGATCATCAAGGAATTTGGAGAAGAGCGC
TGGGCAAAAAAAATAGCCCTGAGAATCGTGCAGACACGCCAGGAAACACCGATCACGACAACGCTTCAGTTGGCTGATCT
TGTAGCCGGCACGATTCCACGCCGTTTTCACGAAGAGCGGATTCATCCTGCAACCCGTACCTTTCAGGCCCTGAGAATTG
CCGTCAATCAGGAGCTTGAACAGGTGGAACAGGGGATCAGGGCAGGGATAGCAGCCCTGAAAGCGGGAGGACGGATTGCC
GTGATCTCCTTCCACTCGCTTGAGGACCGGATCGTGAAGCATCTGTTTCGTGAGGCAGCAACCGGCTGCACCTGTCCCCC
CAGGATGCCCTACTGCGTCTGCAACAAAAAGCCGCAACTACGCATCCTGACCGGACGTCCGGTGATTGCCGGGCCAGAAG
AAACTGACCGCAACCCCCGCGCACGCAGCGCCAAGTTGCGAGCAGCTGAAAAATTGGGCTAG

Upstream 100 bases:

>100_bases
TTTCGACATCTGGGCCAGCGAGACCTACGCACGTGTCAATGCCCAGGATGAGCGCAACTTCCCGCAGGATTCGGCTGCAC
TTGCAGCACTTGGCATATAG

Downstream 100 bases:

>100_bases
TTCCCGAAGGAGGGCTCACTATGGGCATGGTAAAAACCGACAGCACCAAACTTACCTTCCCCGGTCACCTGGGGACTGAT
CTGGCGCCACGCCGGATGGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEEFHHLSVLAREVMEQLAPRPGGTYLDGTLGGGGHSELILEKIGPDGLLIGIDRDPAALAAASERLRRFGSCFRPLQGS
FGDLAELLKQEGINTLDGLLLDLGVSSHQLDTDERGFSFRLDGPLDMRMDRSCGDSAADLLQDCSAGELEQIIKEFGEER
WAKKIALRIVQTRQETPITTTLQLADLVAGTIPRRFHEERIHPATRTFQALRIAVNQELEQVEQGIRAGIAALKAGGRIA
VISFHSLEDRIVKHLFREAATGCTCPPRMPYCVCNKKPQLRILTGRPVIAGPEETDRNPRARSAKLRAAEKLG

Sequences:

>Translated_313_residues
MEEFHHLSVLAREVMEQLAPRPGGTYLDGTLGGGGHSELILEKIGPDGLLIGIDRDPAALAAASERLRRFGSCFRPLQGS
FGDLAELLKQEGINTLDGLLLDLGVSSHQLDTDERGFSFRLDGPLDMRMDRSCGDSAADLLQDCSAGELEQIIKEFGEER
WAKKIALRIVQTRQETPITTTLQLADLVAGTIPRRFHEERIHPATRTFQALRIAVNQELEQVEQGIRAGIAALKAGGRIA
VISFHSLEDRIVKHLFREAATGCTCPPRMPYCVCNKKPQLRILTGRPVIAGPEETDRNPRARSAKLRAAEKLG
>Mature_313_residues
MEEFHHLSVLAREVMEQLAPRPGGTYLDGTLGGGGHSELILEKIGPDGLLIGIDRDPAALAAASERLRRFGSCFRPLQGS
FGDLAELLKQEGINTLDGLLLDLGVSSHQLDTDERGFSFRLDGPLDMRMDRSCGDSAADLLQDCSAGELEQIIKEFGEER
WAKKIALRIVQTRQETPITTTLQLADLVAGTIPRRFHEERIHPATRTFQALRIAVNQELEQVEQGIRAGIAALKAGGRIA
VISFHSLEDRIVKHLFREAATGCTCPPRMPYCVCNKKPQLRILTGRPVIAGPEETDRNPRARSAKLRAAEKLG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=342, Percent_Identity=40.6432748538012, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=45.5026455026455, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=47.1698113207547, Blast_Score=266, Evalue=1e-72,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=40.4255319148936, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=40.4255319148936, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_GEOLS (B3E3Z0)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001950924.1
- ProteinModelPortal:   B3E3Z0
- SMR:   B3E3Z0
- GeneID:   6369323
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0678
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34401; Mature: 34401

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEFHHLSVLAREVMEQLAPRPGGTYLDGTLGGGGHSELILEKIGPDGLLIGIDRDPAAL
CCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHH
AAASERLRRFGSCFRPLQGSFGDLAELLKQEGINTLDGLLLDLGVSSHQLDTDERGFSFR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
LDGPLDMRMDRSCGDSAADLLQDCSAGELEQIIKEFGEERWAKKIALRIVQTRQETPITT
ECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TLQLADLVAGTIPRRFHEERIHPATRTFQALRIAVNQELEQVEQGIRAGIAALKAGGRIA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VISFHSLEDRIVKHLFREAATGCTCPPRMPYCVCNKKPQLRILTGRPVIAGPEETDRNPR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCEEECCCCCCCCCC
ARSAKLRAAEKLG
HHHHHHHHHHHCC
>Mature Secondary Structure
MEEFHHLSVLAREVMEQLAPRPGGTYLDGTLGGGGHSELILEKIGPDGLLIGIDRDPAAL
CCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHH
AAASERLRRFGSCFRPLQGSFGDLAELLKQEGINTLDGLLLDLGVSSHQLDTDERGFSFR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
LDGPLDMRMDRSCGDSAADLLQDCSAGELEQIIKEFGEERWAKKIALRIVQTRQETPITT
ECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TLQLADLVAGTIPRRFHEERIHPATRTFQALRIAVNQELEQVEQGIRAGIAALKAGGRIA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VISFHSLEDRIVKHLFREAATGCTCPPRMPYCVCNKKPQLRILTGRPVIAGPEETDRNPR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEEEEECCCEEECCCCCCCCCC
ARSAKLRAAEKLG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA