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The map label for this gene is murF [H]

Identifier: 189423743

GI number: 189423743

Start: 681703

End: 683091

Strand: Reverse

Name: murF [H]

Synonym: Glov_0674

Alternate gene names: 189423743

Gene position: 683091-681703 (Counterclockwise)

Preceding gene: 189423744

Following gene: 189423742

Centisome position: 17.44

GC content: 58.89

Gene sequence:

>1389_bases
ATGTTCACCCCGGAACAACTGACAACCATCACCCATGGTCGATTGATCGGGCCTGAAACCGCATCGGTCAGCGGCATCTC
AACTGACTCGCGTACCATCAAACCTGGTGAGCTGTTCATACCGTTACGGGGAGACCGCTTTGACGGCCACGACTATCTCA
ACGGGCTCACGGAGCGACGGATCCAGGTCAGCCTGTGCAATGAGTCATGGCTGCAACATCAGCAACCTCCGGCGACCTTA
AGCTGCATTGCCGTACGCGACACCCTGCGCGCACTGGGGGATCTTGCCGCCGCCTTCCGGAAACGGTTTGCCCTGACCAT
GATCGGGGTCACCGGCAGTAATGGCAAAACCACCACCAAAGAGATGCTGGCAGCCATTCTCGACCAGGTTGACCAGGGCC
TGAAAACCAGCGGCAACCTGAACAATCAGATCGGCCTACCGCAGATGCTGTTTCAGCTGAAACCGGAACATTGCTGGGCA
GTGCTGGAGATGGGGATGAGTGAGCCGGGTGAGATTGATCGCCTGGCAGAGATCGCCTGTCCTTCAATCGGCATTGTTCT
CAATGCCTTTCCGGCCCACCTGCAGAGTATGGGGAGTGTCGAGGCCGTGGCTCAGGCCAAGGGTGAACTGCTACAGCGGA
TACAGGATGGCGGCCTTGCAGTCGTGAATGCCGATGACCCCCGTATCGCCTCGCTGCACCAGAACCCTTCTGCCCGCAGG
ACCAGCTTTGGCATCAAACGCGGCGAGGTTCGTGCTGAAGCGGTGCAGGCAATCGGCACAGAAGGTCAGGCATTCAAGCT
GGTTACTCCCAAAGGCAGCATCGAGCTGCGGCTCAAGGCCCTGGGACAGCACAACATCTACAACGCCCTAGCTGCAGCAG
CCGGATTACTGGAGCTGGTCGGCCTGGAAACCATTGCCGAAGGGCTCGAGGCCTTCGTTCCCTACACCGGACGCTTCCAT
CTGGAAACGCTGTCCAACAACAGGCTGCTGATTGATGACAGCTACAATGCAAACCCAGCCTCCTGCGCTGCGGCCCTGGC
AACCCTGCAGGGAATCAAAGGCAACAGACGCGCCTTCGTGGCATTGGGGGACATGCTGGAACTGGGCAGAGACGAGCTGG
AGCTGCATCGTCAGTTAGGCATCCAGGCCGCTTCGGTTGCGGACCGGCTCTATCTGCTGGGCAGTCTTACCAAAGAAACC
GCCGGTGCAGCCGCAGAGGCAGGCTTGGCTTCAGAGGCGATCATCTCTGCAACAAGCCACGAAGAGATCGCCAGGGATAT
ACATACCCATGCAGGCAACGGCGACCTGATCTTGCTTAAGGGTTCACGGGGGATGAAGATGGAAAAAATCGCCGCGCTGC
TGCGGGCTGCAGACGGCCAGGAGGACTAA

Upstream 100 bases:

>100_bases
GCAGGCAAGGGGCATGAAGATTACCAGATCATCGGCCAGACCAAACAACACTTTGACGACCGTGAGGAAGCAGCCAATGC
ATTCAGGGAGCGGATCTGAC

Downstream 100 bases:

>100_bases
CACCATGCTCTTTCACCTGTTCTACCCCCTGGCATCCAACCTGAAGATTCTGAACATCTTCAAGTATCTGACCTTCAGAA
CCATCTATGCCATGATCACG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MFTPEQLTTITHGRLIGPETASVSGISTDSRTIKPGELFIPLRGDRFDGHDYLNGLTERRIQVSLCNESWLQHQQPPATL
SCIAVRDTLRALGDLAAAFRKRFALTMIGVTGSNGKTTTKEMLAAILDQVDQGLKTSGNLNNQIGLPQMLFQLKPEHCWA
VLEMGMSEPGEIDRLAEIACPSIGIVLNAFPAHLQSMGSVEAVAQAKGELLQRIQDGGLAVVNADDPRIASLHQNPSARR
TSFGIKRGEVRAEAVQAIGTEGQAFKLVTPKGSIELRLKALGQHNIYNALAAAAGLLELVGLETIAEGLEAFVPYTGRFH
LETLSNNRLLIDDSYNANPASCAAALATLQGIKGNRRAFVALGDMLELGRDELELHRQLGIQAASVADRLYLLGSLTKET
AGAAAEAGLASEAIISATSHEEIARDIHTHAGNGDLILLKGSRGMKMEKIAALLRAADGQED

Sequences:

>Translated_462_residues
MFTPEQLTTITHGRLIGPETASVSGISTDSRTIKPGELFIPLRGDRFDGHDYLNGLTERRIQVSLCNESWLQHQQPPATL
SCIAVRDTLRALGDLAAAFRKRFALTMIGVTGSNGKTTTKEMLAAILDQVDQGLKTSGNLNNQIGLPQMLFQLKPEHCWA
VLEMGMSEPGEIDRLAEIACPSIGIVLNAFPAHLQSMGSVEAVAQAKGELLQRIQDGGLAVVNADDPRIASLHQNPSARR
TSFGIKRGEVRAEAVQAIGTEGQAFKLVTPKGSIELRLKALGQHNIYNALAAAAGLLELVGLETIAEGLEAFVPYTGRFH
LETLSNNRLLIDDSYNANPASCAAALATLQGIKGNRRAFVALGDMLELGRDELELHRQLGIQAASVADRLYLLGSLTKET
AGAAAEAGLASEAIISATSHEEIARDIHTHAGNGDLILLKGSRGMKMEKIAALLRAADGQED
>Mature_462_residues
MFTPEQLTTITHGRLIGPETASVSGISTDSRTIKPGELFIPLRGDRFDGHDYLNGLTERRIQVSLCNESWLQHQQPPATL
SCIAVRDTLRALGDLAAAFRKRFALTMIGVTGSNGKTTTKEMLAAILDQVDQGLKTSGNLNNQIGLPQMLFQLKPEHCWA
VLEMGMSEPGEIDRLAEIACPSIGIVLNAFPAHLQSMGSVEAVAQAKGELLQRIQDGGLAVVNADDPRIASLHQNPSARR
TSFGIKRGEVRAEAVQAIGTEGQAFKLVTPKGSIELRLKALGQHNIYNALAAAAGLLELVGLETIAEGLEAFVPYTGRFH
LETLSNNRLLIDDSYNANPASCAAALATLQGIKGNRRAFVALGDMLELGRDELELHRQLGIQAASVADRLYLLGSLTKET
AGAAAEAGLASEAIISATSHEEIARDIHTHAGNGDLILLKGSRGMKMEKIAALLRAADGQED

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=35.9307359307359, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49398; Mature: 49398

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPEQLTTITHGRLIGPETASVSGISTDSRTIKPGELFIPLRGDRFDGHDYLNGLTERR
CCCCHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCE
IQVSLCNESWLQHQQPPATLSCIAVRDTLRALGDLAAAFRKRFALTMIGVTGSNGKTTTK
EEEEECCHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHH
EMLAAILDQVDQGLKTSGNLNNQIGLPQMLFQLKPEHCWAVLEMGMSEPGEIDRLAEIAC
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHC
PSIGIVLNAFPAHLQSMGSVEAVAQAKGELLQRIQDGGLAVVNADDPRIASLHQNPSARR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHH
TSFGIKRGEVRAEAVQAIGTEGQAFKLVTPKGSIELRLKALGQHNIYNALAAAAGLLELV
HHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
GLETIAEGLEAFVPYTGRFHLETLSNNRLLIDDSYNANPASCAAALATLQGIKGNRRAFV
HHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
ALGDMLELGRDELELHRQLGIQAASVADRLYLLGSLTKETAGAAAEAGLASEAIISATSH
EEHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCH
EEIARDIHTHAGNGDLILLKGSRGMKMEKIAALLRAADGQED
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFTPEQLTTITHGRLIGPETASVSGISTDSRTIKPGELFIPLRGDRFDGHDYLNGLTERR
CCCCHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCE
IQVSLCNESWLQHQQPPATLSCIAVRDTLRALGDLAAAFRKRFALTMIGVTGSNGKTTTK
EEEEECCHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHH
EMLAAILDQVDQGLKTSGNLNNQIGLPQMLFQLKPEHCWAVLEMGMSEPGEIDRLAEIAC
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHC
PSIGIVLNAFPAHLQSMGSVEAVAQAKGELLQRIQDGGLAVVNADDPRIASLHQNPSARR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCHH
TSFGIKRGEVRAEAVQAIGTEGQAFKLVTPKGSIELRLKALGQHNIYNALAAAAGLLELV
HHCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
GLETIAEGLEAFVPYTGRFHLETLSNNRLLIDDSYNANPASCAAALATLQGIKGNRRAFV
HHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
ALGDMLELGRDELELHRQLGIQAASVADRLYLLGSLTKETAGAAAEAGLASEAIISATSH
EEHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCCCCH
EEIARDIHTHAGNGDLILLKGSRGMKMEKIAALLRAADGQED
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]