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The map label for this gene is murD

Identifier: 189423741

GI number: 189423741

Start: 679280

End: 680620

Strand: Reverse

Name: murD

Synonym: Glov_0672

Alternate gene names: 189423741

Gene position: 680620-679280 (Counterclockwise)

Preceding gene: 189423742

Following gene: 189423740

Centisome position: 17.37

GC content: 57.05

Gene sequence:

>1341_bases
ATGGAACTACGCACTAAAAAAACAACTGTCATGGGCCTCGCCAAAACCGGTGTGGCCTCTGCCCGTTTTCTGGCACAGCA
GGGTGCACGGGTAACCGCCACTGACATGCGGGATGAAACCGCTCTGGCAACAGTGTTGTCAGAACTGGCAGGGCTGGATA
TCCGCTTTGTGTTGGGACATCATGATGAGGCCGATTTTACAGCGGCTGATCTGGTGGTGGTTTCCCCCGGTGTACCGCAG
GAGCACCCCCTGCTGCGTAAGACAACTGAAGCCGGGGTTGCCGTGGTGAGCGAGATCGAACTGGCCAGCCGTTTTATCAC
GGCGCCATTGGTGGCCATCACCGGCACCAACGGCAAGACCACCACCACCACCCTGGCGGGCGAGCTGTTCAGGGCCAACG
GCTTCAGCACCTATGTGGGCGGTAACATCGGCGACCCTCTGATTGACCTGCCTGCTTCCGGTGAGCCGGTCGAGCGGGCG
GTTGCGGAAATCAGTTCCTTCCAGCTGGAGTGGATCAGCAGTTTCCGTCCCAAGGTAGCGGCGCTGTTGAACATTACTGA
AGACCACTTGGACCGCTATCCGAGTTATCAGGCATATATCAACGCCAAATTGCGGATCTTTGAAAATCAGACCGCAGATG
ATTTTGCCGTGGTCAACCGGGATGATGAACTGGTCTGGCAGGCTGCCCAATCACTCAAGGCGCAACTGTTCCCGTTCAGC
CGCAGACATGAACTTACTGAAGGGATTTTCAGTCGCGAAGGTGAACTGTTATTCCGACACCACGGCCAGGAATTGAGTAT
CCCGACGGCAGGCTTCAGGCTGCAGGGGGTGCATAATCTGGAAAACATCATGGCTGCCCTGGCCTGCTGCCTGCTGCTGG
GATGTCAGAGAGAGACAAGCCTGGAACTGCTGAACCGTTTTGAGGCGTTGCACCACCGGATGGAGTTTGTGCGTGAGGTG
GCTGATGTGCGCTATTTTGAGGACAGCAAGGCAACTAATGTCGGAAGCGTTGCCAAGGCACTGGAGAGCTTTGAGAACAT
CACCCTGATTGCCGGCGGCAAGGACAAGGGTGGTTCTTATGCACCGCTGGCTGAACTGGTGCAGCAACGGGTCAGGCATC
TGGTGCTGATCGGCGAGGCAGCCGAACGGATGCAGCAGGAGCTTGGGGACTTGACTGCCACGCATAAGGCCGCAACATTG
GAAGAGGCTGTTCAGCTCAGCGCCGACCTGACCGCGCCGGGGGGCGTGGTGCTGTTATCACCGGCCTGCTCCAGTTTTGA
TATGTTCAAGGATTATGAGGAACGGGCCCAGCGCTTTATTACAGCGGTAAAGAGGCTCTAG

Upstream 100 bases:

>100_bases
GAGCTGAAGGGGGTGGCAGAGCCCAAGATCATTGTCCGGTTCTGGATCATCACCATTATTCTGGCCCTGGTGGCCATTTC
AACCCTGAAGATGCGCTGAC

Downstream 100 bases:

>100_bases
ACGATGCTTGAGATCCTGCGCAAACCATTCAGGCTGGCAGAGTATGATCTGGTACTGCTGCTGATGGTGGTGGCGCTGAC
CAGCTTCGGGATCGTGATGG

Product: UDP-N-acetylmuramoylalanine/D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MELRTKKTTVMGLAKTGVASARFLAQQGARVTATDMRDETALATVLSELAGLDIRFVLGHHDEADFTAADLVVVSPGVPQ
EHPLLRKTTEAGVAVVSEIELASRFITAPLVAITGTNGKTTTTTLAGELFRANGFSTYVGGNIGDPLIDLPASGEPVERA
VAEISSFQLEWISSFRPKVAALLNITEDHLDRYPSYQAYINAKLRIFENQTADDFAVVNRDDELVWQAAQSLKAQLFPFS
RRHELTEGIFSREGELLFRHHGQELSIPTAGFRLQGVHNLENIMAALACCLLLGCQRETSLELLNRFEALHHRMEFVREV
ADVRYFEDSKATNVGSVAKALESFENITLIAGGKDKGGSYAPLAELVQQRVRHLVLIGEAAERMQQELGDLTATHKAATL
EEAVQLSADLTAPGGVVLLSPACSSFDMFKDYEERAQRFITAVKRL

Sequences:

>Translated_446_residues
MELRTKKTTVMGLAKTGVASARFLAQQGARVTATDMRDETALATVLSELAGLDIRFVLGHHDEADFTAADLVVVSPGVPQ
EHPLLRKTTEAGVAVVSEIELASRFITAPLVAITGTNGKTTTTTLAGELFRANGFSTYVGGNIGDPLIDLPASGEPVERA
VAEISSFQLEWISSFRPKVAALLNITEDHLDRYPSYQAYINAKLRIFENQTADDFAVVNRDDELVWQAAQSLKAQLFPFS
RRHELTEGIFSREGELLFRHHGQELSIPTAGFRLQGVHNLENIMAALACCLLLGCQRETSLELLNRFEALHHRMEFVREV
ADVRYFEDSKATNVGSVAKALESFENITLIAGGKDKGGSYAPLAELVQQRVRHLVLIGEAAERMQQELGDLTATHKAATL
EEAVQLSADLTAPGGVVLLSPACSSFDMFKDYEERAQRFITAVKRL
>Mature_446_residues
MELRTKKTTVMGLAKTGVASARFLAQQGARVTATDMRDETALATVLSELAGLDIRFVLGHHDEADFTAADLVVVSPGVPQ
EHPLLRKTTEAGVAVVSEIELASRFITAPLVAITGTNGKTTTTTLAGELFRANGFSTYVGGNIGDPLIDLPASGEPVERA
VAEISSFQLEWISSFRPKVAALLNITEDHLDRYPSYQAYINAKLRIFENQTADDFAVVNRDDELVWQAAQSLKAQLFPFS
RRHELTEGIFSREGELLFRHHGQELSIPTAGFRLQGVHNLENIMAALACCLLLGCQRETSLELLNRFEALHHRMEFVREV
ADVRYFEDSKATNVGSVAKALESFENITLIAGGKDKGGSYAPLAELVQQRVRHLVLIGEAAERMQQELGDLTATHKAATL
EEAVQLSADLTAPGGVVLLSPACSSFDMFKDYEERAQRFITAVKRL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=455, Percent_Identity=36.2637362637363, Blast_Score=234, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_GEOLS (B3E3Y4)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001950918.1
- ProteinModelPortal:   B3E3Y4
- SMR:   B3E3Y4
- GeneID:   6369248
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0672
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK829090
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48826; Mature: 48826

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRTKKTTVMGLAKTGVASARFLAQQGARVTATDMRDETALATVLSELAGLDIRFVLGH
CCCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECC
HDEADFTAADLVVVSPGVPQEHPLLRKTTEAGVAVVSEIELASRFITAPLVAITGTNGKT
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLAGELFRANGFSTYVGGNIGDPLIDLPASGEPVERAVAEISSFQLEWISSFRPKVA
EHHHHHHHHHHCCCCCEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
ALLNITEDHLDRYPSYQAYINAKLRIFENQTADDFAVVNRDDELVWQAAQSLKAQLFPFS
HHHHCCHHHHHCCCCEEEEEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCH
RRHELTEGIFSREGELLFRHHGQELSIPTAGFRLQGVHNLENIMAALACCLLLGCQRETS
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCHH
LELLNRFEALHHRMEFVREVADVRYFEDSKATNVGSVAKALESFENITLIAGGKDKGGSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCC
APLAELVQQRVRHLVLIGEAAERMQQELGDLTATHKAATLEEAVQLSADLTAPGGVVLLS
CHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
PACSSFDMFKDYEERAQRFITAVKRL
CCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRTKKTTVMGLAKTGVASARFLAQQGARVTATDMRDETALATVLSELAGLDIRFVLGH
CCCCCCHHHHHHHHHHCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECC
HDEADFTAADLVVVSPGVPQEHPLLRKTTEAGVAVVSEIELASRFITAPLVAITGTNGKT
CCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCE
TTTTLAGELFRANGFSTYVGGNIGDPLIDLPASGEPVERAVAEISSFQLEWISSFRPKVA
EHHHHHHHHHHCCCCCEECCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
ALLNITEDHLDRYPSYQAYINAKLRIFENQTADDFAVVNRDDELVWQAAQSLKAQLFPFS
HHHHCCHHHHHCCCCEEEEEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCH
RRHELTEGIFSREGELLFRHHGQELSIPTAGFRLQGVHNLENIMAALACCLLLGCQRETS
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCHH
LELLNRFEALHHRMEFVREVADVRYFEDSKATNVGSVAKALESFENITLIAGGKDKGGSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCC
APLAELVQQRVRHLVLIGEAAERMQQELGDLTATHKAATLEEAVQLSADLTAPGGVVLLS
CHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
PACSSFDMFKDYEERAQRFITAVKRL
CCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA