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The map label for this gene is nifH [H]

Identifier: 189423719

GI number: 189423719

Start: 656323

End: 657189

Strand: Reverse

Name: nifH [H]

Synonym: Glov_0650

Alternate gene names: 189423719

Gene position: 657189-656323 (Counterclockwise)

Preceding gene: 189423720

Following gene: 189423718

Centisome position: 16.77

GC content: 54.44

Gene sequence:

>867_bases
ATGAGACAGATAGCTATTTACGGCAAAGGCGGCATCGGCAAATCAACCACCACACAAAACACGGTGGCAGGCCTGGCATC
CATCGGCAAGAAGGTCATGATAGTCGGTTGCGACCCCAAGGCAGACTCCACCCGTCTGATCCTGCATGCCAAGGCACAGA
ATACGGTTATGGACCTGGTTCGTGAACTGGGTACGGTTGAAGACCTTGAGCTGGAAGAAGTGATGAAGGTCGGCTACGGC
GATGTAAAGTGTGTCGAGTCCGGCGGACCTGAGCCTGGCGTTGGTTGTGCAGGCCGTGGTGTTATTACCGCCATCAACTT
TCTGGAGGAAAACGGTGCCTATACCCCTGACCTTGATTTTGTTTTCTATGACGTGCTTGGTGACGTTGTCTGCGGCGGTT
TCGCCATGCCGATCCGTGAAGGCAAGGCAGAAGAGATCTACATTGTCTGCTCCGGCGAAATGATGGCCATGTACGCTGCC
AACAACATTGCCAAAGGTATTCTGAAATATGCCTCCTCCGGCAAGGTCCGCCTGGGCGGCCTGATCTGTAACTCCCGCAA
CACTGACCGCGAAGACGAACTGATTGAGGCTTTGGCTGCCCGTCTCGGCACCCAGATGATCCATTTTGTCCCCCGTGACA
ATCAGGTCCAACGGGCCGAGCTGCGCCGGATGACCGTTATTGAATACTCTCCTGACCACAAGCAGGCCAACGAATACCGT
ACCCTGGCCCAGAAAATTGCCGACAACAAGATGCTGGTAGTGCCTACTCCGCTTGAGATGGAAGAGCTGGAAGACCTGCT
GATGGAGTTCGGCATCATGGAAGTGGATGATGAAGAAAACGTCGGCAAAGTGGAAGGCGCAGCCTAG

Upstream 100 bases:

>100_bases
GCGCCTTCCAATTTAGTTCACACAATTGGGGCGCCGTTTTGCGTTGCCCCGACCAACATCCCAAGGGACCCACAGATGAG
TGGGTGAAAGGAGCAGTACC

Downstream 100 bases:

>100_bases
GAAACGGTCTTTTTCCGCAGTCTCGGCGTTAGGTTCCTGATTCGGTTGTGCGGCGCACTGCTTCGTACACCTCCCTCGAA
CCGGCATCATCCGCCTTGAC

Product: nitrogenase iron protein

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELEEVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLGGLICNSRNTDREDELIEALAARLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHKQANEYR
TLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEVDDEENVGKVEGAA

Sequences:

>Translated_288_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELEEVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLGGLICNSRNTDREDELIEALAARLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHKQANEYR
TLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEVDDEENVGKVEGAA
>Mature_288_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELEEVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLGGLICNSRNTDREDELIEALAARLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHKQANEYR
TLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEVDDEENVGKVEGAA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31332; Mature: 31332

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQNTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHH
RELGTVEDLELEEVMKVGYGDVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDF
HHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAANNIAKGILKYASSGKVRLGG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
LICNSRNTDREDELIEALAARLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHKQANEYR
EEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEEEEEEECCCCHHHHHHH
TLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEVDDEENVGKVEGAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQNTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHH
RELGTVEDLELEEVMKVGYGDVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDF
HHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAANNIAKGILKYASSGKVRLGG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
LICNSRNTDREDELIEALAARLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHKQANEYR
EEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEEEEEEECCCCHHHHHHH
TLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEVDDEENVGKVEGAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA