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The map label for this gene is nifD [H]

Identifier: 189423718

GI number: 189423718

Start: 654730

End: 656175

Strand: Reverse

Name: nifD [H]

Synonym: Glov_0649

Alternate gene names: 189423718

Gene position: 656175-654730 (Counterclockwise)

Preceding gene: 189423719

Following gene: 189423717

Centisome position: 16.75

GC content: 54.22

Gene sequence:

>1446_bases
ATGTCAGACAAGATACGTAAAATAGAGGGCATCACCAAAGAATCCACCGAGGCGCTGATAGCTGAAACCTTGTCGGTGTA
TCCGGAAAAGGCCAGAAAAAAACGCGCTCCGCATATCGGTGCCAACGACCAGGGATCAGGTTCAGCCTGTGTCAAGTCCA
ACAAGAAGACGGTTCCCGGCGTTATGTCTGCCCGTGGCTGCGCCTACGCAGGTGCCAAAGGGGTGGTTTGGGGACCGATC
CGCGACATGGTTCACGTTTCCCACGGCCCGGTTGGCTGCGGCTGGTATTCATGGGGTACCCGGCGTAACATGGTAAGCGG
CATTGTCGGCGTTACCAGTTTTCCGATGCAGTTCACCTCCGACTTCCAGGAAAAGGATATTGTCTACGGTGGCGATAAAA
AGCTGAAGACCCTGCTGGAAGAGGCCCACGACCTGTTTCCGCTGGCCAAAGGGATCTCGGTACTGTCCGAATGCCCGGTC
GGCCTGATCGGTGACGACATCAACTCCGTGGCCAAGACTTCTGCCAAGGAGCTGGATCTGCCGATCATCCCCTGTAACTG
CGAAGGTTTCCGTGGTGTTTCCCAATCACTTGGCCACCACATCAGTAACGACACCATCCGTGACTATATCATCGGCACCC
GTGAGTACGCCGAACCTGAATCGCCCTATGACATCGCTCTGATCGGTGAATACAACATCGGTGGTGACGCCTGGTCCACC
AAGCCGCTGTTGGAAGAGTGTGGCTTTAACGTCAAAGCGGTCTGGACCGGCGATGGTGAACTGGAAAAGATTGCGGCCAC
CCATACCGTAAAGCTCAACGTAATCCACTGTTACCGTTCCATGAACTATATGTGCAAGGTGATGGAAGAAAAATTCGGCA
TTCCCTGGATTGAGCTGAACTTCTTCGGCCCGACCAAGATCAAGGAAAGCCTGCGTAAGCTGGCAGCCCTGTTTGACGAC
ACCATCAAGGAGAAGGTTGAGGCGGTAATCGCCAAGTATGATCCGATCATGCAGGGAATTATCGAGCAGTACAAGCCACG
CCTGGACGGCAAAAAGGTAATGCTGCTTGTGGGTGGTCTCCGTCCACGTCACACCATCGGCGCCTACGAAGATCTTGGCA
TGGACTGCATCGGTTCCGGCTATGAATTTGCCCACACCGATGACTATGACCGTACCGCACCCGAGATGCCTGATGCCACG
GTGGTGTACGACGATCCATCCGAATACGAGTTTGAAAAGTTTGCCGATGCCCTGAAGCCGGACCTGATCGGCTCCGGTAT
CAAGGAAAAATATGTCTTCCAGAAAATGGGCATTCCGTTCCGTCAGATGCACAGCTGGGACTACTCCGGCCCCTACCACG
GCTACAAAGGCTTTGAGGTATTTGCACGCGACGTGGATATGGCAATCAACAGCCCGACCTGGAAGCTGGTGCAGTCACCA
TTCTAA

Upstream 100 bases:

>100_bases
GTGCGGCGCACTGCTTCGTACACCTCCCTCGAACCGGCATCATCCGCCTTGACCTTGTGAAAAAGTCCCCGTTTTTCTGA
ACATCTCAAGGAGAATACCT

Downstream 100 bases:

>100_bases
CGCTGTCAGAACACGCACGATACTTACCTACAATGGCGGTGTTACGGATGAGTTTCCCGCCAAAGGAGATATACCATGTC
AAACTTACTAGGACTCGAAG

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSDKIRKIEGITKESTEALIAETLSVYPEKARKKRAPHIGANDQGSGSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNMVSGIVGVTSFPMQFTSDFQEKDIVYGGDKKLKTLLEEAHDLFPLAKGISVLSECPV
GLIGDDINSVAKTSAKELDLPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIGTREYAEPESPYDIALIGEYNIGGDAWST
KPLLEECGFNVKAVWTGDGELEKIAATHTVKLNVIHCYRSMNYMCKVMEEKFGIPWIELNFFGPTKIKESLRKLAALFDD
TIKEKVEAVIAKYDPIMQGIIEQYKPRLDGKKVMLLVGGLRPRHTIGAYEDLGMDCIGSGYEFAHTDDYDRTAPEMPDAT
VVYDDPSEYEFEKFADALKPDLIGSGIKEKYVFQKMGIPFRQMHSWDYSGPYHGYKGFEVFARDVDMAINSPTWKLVQSP
F

Sequences:

>Translated_481_residues
MSDKIRKIEGITKESTEALIAETLSVYPEKARKKRAPHIGANDQGSGSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPI
RDMVHVSHGPVGCGWYSWGTRRNMVSGIVGVTSFPMQFTSDFQEKDIVYGGDKKLKTLLEEAHDLFPLAKGISVLSECPV
GLIGDDINSVAKTSAKELDLPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIGTREYAEPESPYDIALIGEYNIGGDAWST
KPLLEECGFNVKAVWTGDGELEKIAATHTVKLNVIHCYRSMNYMCKVMEEKFGIPWIELNFFGPTKIKESLRKLAALFDD
TIKEKVEAVIAKYDPIMQGIIEQYKPRLDGKKVMLLVGGLRPRHTIGAYEDLGMDCIGSGYEFAHTDDYDRTAPEMPDAT
VVYDDPSEYEFEKFADALKPDLIGSGIKEKYVFQKMGIPFRQMHSWDYSGPYHGYKGFEVFARDVDMAINSPTWKLVQSP
F
>Mature_480_residues
SDKIRKIEGITKESTEALIAETLSVYPEKARKKRAPHIGANDQGSGSACVKSNKKTVPGVMSARGCAYAGAKGVVWGPIR
DMVHVSHGPVGCGWYSWGTRRNMVSGIVGVTSFPMQFTSDFQEKDIVYGGDKKLKTLLEEAHDLFPLAKGISVLSECPVG
LIGDDINSVAKTSAKELDLPIIPCNCEGFRGVSQSLGHHISNDTIRDYIIGTREYAEPESPYDIALIGEYNIGGDAWSTK
PLLEECGFNVKAVWTGDGELEKIAATHTVKLNVIHCYRSMNYMCKVMEEKFGIPWIELNFFGPTKIKESLRKLAALFDDT
IKEKVEAVIAKYDPIMQGIIEQYKPRLDGKKVMLLVGGLRPRHTIGAYEDLGMDCIGSGYEFAHTDDYDRTAPEMPDATV
VYDDPSEYEFEKFADALKPDLIGSGIKEKYVFQKMGIPFRQMHSWDYSGPYHGYKGFEVFARDVDMAINSPTWKLVQSPF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 53423; Mature: 53291

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00435 PEROXIDASE_1 ; PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKIRKIEGITKESTEALIAETLSVYPEKARKKRAPHIGANDQGSGSACVKSNKKTVPG
CCCHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNMVSGIVGVTSFPMQFTS
HHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHC
DFQEKDIVYGGDKKLKTLLEEAHDLFPLAKGISVLSECPVGLIGDDINSVAKTSAKELDL
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHCCC
PIIPCNCEGFRGVSQSLGHHISNDTIRDYIIGTREYAEPESPYDIALIGEYNIGGDAWST
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCCEEEEEEECCCCCCCCCC
KPLLEECGFNVKAVWTGDGELEKIAATHTVKLNVIHCYRSMNYMCKVMEEKFGIPWIELN
CHHHHHCCCCEEEEECCCCCHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPTKIKESLRKLAALFDDTIKEKVEAVIAKYDPIMQGIIEQYKPRLDGKKVMLLVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
RPRHTIGAYEDLGMDCIGSGYEFAHTDDYDRTAPEMPDATVVYDDPSEYEFEKFADALKP
CCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCH
DLIGSGIKEKYVFQKMGIPFRQMHSWDYSGPYHGYKGFEVFARDVDMAINSPTWKLVQSP
HHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCC
F
C
>Mature Secondary Structure 
SDKIRKIEGITKESTEALIAETLSVYPEKARKKRAPHIGANDQGSGSACVKSNKKTVPG
CCHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCC
VMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNMVSGIVGVTSFPMQFTS
HHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHC
DFQEKDIVYGGDKKLKTLLEEAHDLFPLAKGISVLSECPVGLIGDDINSVAKTSAKELDL
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHCCHHHCCC
PIIPCNCEGFRGVSQSLGHHISNDTIRDYIIGTREYAEPESPYDIALIGEYNIGGDAWST
CEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHHCCCCCCCEEEEEEECCCCCCCCCC
KPLLEECGFNVKAVWTGDGELEKIAATHTVKLNVIHCYRSMNYMCKVMEEKFGIPWIELN
CHHHHHCCCCEEEEECCCCCHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
FFGPTKIKESLRKLAALFDDTIKEKVEAVIAKYDPIMQGIIEQYKPRLDGKKVMLLVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
RPRHTIGAYEDLGMDCIGSGYEFAHTDDYDRTAPEMPDATVVYDDPSEYEFEKFADALKP
CCCCCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCH
DLIGSGIKEKYVFQKMGIPFRQMHSWDYSGPYHGYKGFEVFARDVDMAINSPTWKLVQSP
HHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3344210; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]