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The map label for this gene is nifE [H]

Identifier: 189423708

GI number: 189423708

Start: 641024

End: 643771

Strand: Reverse

Name: nifE [H]

Synonym: Glov_0639

Alternate gene names: 189423708

Gene position: 643771-641024 (Counterclockwise)

Preceding gene: 189423712

Following gene: 189423703

Centisome position: 16.43

GC content: 58.92

Gene sequence:

>2748_bases
ATGGCAAAACCTGACTACTACGACGTTGAAGACTGTTCCACCCACGAAAAGGGTGCTCCAAAGTTCTGTAAAAAATCCGA
GCCTGGCGAGGGAACCGAACGTTCCTGCGCCTATGACGGCGCCCGGGTAGTGTTGATGCCGGTGACCGACGCGATTCACC
TGGTGCATGGCCCGATCGCCTGCGCCGGCAACTCCTGGGACAACCGGGGGGCCCGTTCATCCGGCTCACAACTGTACCGC
CGTGGTTTCACCACCGAGATGCTGGAAAACGATGTGATCTTTGGTGGTGAGAAGAAGCTGTACAAGGCGATCATTGATCT
GGCCGAACGCTACCCTGAAGCAAAAGGGATGTTCGTCTACGCCACCTGCGTCACCGCCATGACCGGTGATGATATTGAAT
CGGTCTGCAAGGCAGCCCAGGAAAAGGTCAGCATCCCGCTGGTGCCGGTCAACACCCCCGGCTTTATCGGCGACAAGAAC
ATCGGCAACCGCCTGGCTGGTGAAATTCTGTTCAAACAGGTGATCGGTACGGCAGAGCCACCAACGCTGGGTGAATATCC
GATCAACCTGATCGGCGAATACAACATCGCCGGTGACCTGTGGGGGATGCTGCCGCTGTTTGACCGGCTGGGTATCCAGA
TCCTCTCCTGTTTTTCCGGCGATGCCAAGTTTGAGGAGCTGCGCTACGCCCACCGGGCCAAGCTGAACATTATCATCTGC
TCCAAGAGCCTGACCAACCTGGCCAAGAAGATGCAAAAGACCTACGGCATGCCGTATCTGGAAGAGTCGTTCTACGGCAT
GACCGATATTGCCAAGGCGCTGCGCAACATCGCCATTGCCCTGGATGACGCAGTCAACGGTCTTGAAAAGCGGATCATGC
AGGATCGGGTGGAGCAGCTGCTGGAGGAGGAAGAGGCAAAGTGCCGCGAGCGGCTGGCCCCCTACCGCGCCCGACTGGAA
GGGAAGCGGGCCGTGCTGTTCACCGGCGGGGTCAAGACCTGGTCCATGGTCAATGCCCTGCGTGAGCTGGGGGTAGAGAT
CCTGGCTGCCGGAACCCAGAACTCCACCCTTGAAGACTTCTACCGGATGAAGGCCTTGATGCACAAGGATGCCAGGATCA
TTGAAGACACCTCCACCGCCGGTCTGCTGGGGGTGATGAAAGAAAAGATGCCGGATCTGATTGTAGCTGGCGGCAAGACC
AAGTTTCTGGCCTTAAAGACCAAGACGCCGTTCCTTGACATCAACCATGGCCGCTCCCACCCCTATGCCGGGTATGAGGG
GATGGTGACCTTTGCAAAACAGCTGGACCTGACCGTTAACAACCCGATCTGGACGCTGCTGAACAGCCCGGAACCGTGGG
AGACCGATGCAACCCCGCTGGTAAATGCTGCCCGCAGCCATGCTGCCACCTATCTGGCAGAGGATCTGGCTGCATCACGG
GTAGCAGTGCCGAAAAAGGCTGCCACGGTCAACCCACAAAAAAACTCGCCAGCCCTGGGGGCAACCCTGGCCTATCTGGG
AATTGATCAGATGCTGGGGCTGCTGCATGGTGCGCAGGGCTGTTCCACCTTTATCCGGTTGCAGCTCTCCAGGCACTTCA
AGGAATCCATCGCCCTGAACGCCACGGCCATGAGCGAAGACAGTGCCATCTTTGGCGGCTGGGAAAACCTGAAAAAAGGG
ATCGGCCGGGTGATCGAGAAATTCGGTCCCAAGGTGGTGGGGGTGATGACCTCCGGCCTGACCGAGACCATGGGGGATGA
TGTCCGCTCTGCCATCCACCAGTTCCGTGTGGAGCATCCGGAGCATGACCAGGTACCGATTATCTGGGCCTCAACCCCGG
ATTACTGCGGCTCATTGCAGGAAGGGTATGCCAACACCGTCGAGGCGATTCTGGCCACCCTGCCGGAGGCTGGTGAACCG
ATCCCGGGTCAGGTCAACCTGGTGCCGGGGGCGCAATTGACACCGGCAGATGTGGAGGAGCTGAAGGAGCTGATCGAATC
ATTCGGCCTGAGCGTACTCTGTGTTCCGGACATCGCCAATGCCCTGGATGGCCATATTGATGCCGATGTATCACCCCTTT
CCACCGGCGGCATCAGCGTCGACAAGATCAGACAGGCCGGCCGCAGTATCGCCACCCTGTATGTGGGTGATTCACTGGCC
AGGGCAGCCAGCCAGCTGGAAGAACGGCTGGGGATTCCGGCCTACGGCTTCACCTCCTTGACCGGCATGGCCGAGACTGA
CCACTTCATGTCAACCCTGTCAGCCATCAGTGGCAAAGCAATACCGGAGAAACAGCAGCGTTGGAGATCAAGATTGGCTG
ATGCGCTGGTGGACAGCCACTACCAGTTTGGCAACAAAAAGGTTGCCATCGCCCTTGAGGCCGACCATCTGAAAGGGATG
ACCCGTTTTCTGGCAGGCATGGGCTGCCAGATCCAGGCTGCCCTGGCAGCCACCCGCACCCGCGGGCTGGACAGCCTGCC
CTGTAACAACGTCTTTGTGGGTGACCTTGAGGATCTTGAAACCGCTGCCGTCGGTGCCGACCTGCTGGTGGCCAACTCCA
ACGGCAGGCAGGCAGCAGCCAAGCTCAAGATCGGCGCCCACCTCAGAACCGGCCTGCCGGTGTTTGACCGGTTGGGGGCG
CACCAGAAGATGTGGGTCGGCTATCGCGGTACCCTGAACCTGGTGTTTGAAGTGGCAAACCTGTTCCAGGCCAACGCAAA
GGACGCCCAGAAACTGGCACATAACTGA

Upstream 100 bases:

>100_bases
TCGGCACATATTCAAGCAAAAACAAATAATTACACTATTGGCACGCCCAGTGCAAAGTCAGGTCACGGGCAGCAACGATG
CCGCCCGTTTGGAGGCAGCA

Downstream 100 bases:

>100_bases
AAAGCAGGTTCTGAAAAAAGGACACAGAAAAGCCGCGTTACTGTTTAACGCGGCTTTTCTTGTGGCTAATCAGACGCTTT
TAGAAATTCTGAAATTCCGA

Product: bifunctional nitrogenase molybdenum-cofactor biosynthesis protein NifE/NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 915; Mature: 914

Protein sequence:

>915_residues
MAKPDYYDVEDCSTHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDAIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVIFGGEKKLYKAIIDLAERYPEAKGMFVYATCVTAMTGDDIESVCKAAQEKVSIPLVPVNTPGFIGDKN
IGNRLAGEILFKQVIGTAEPPTLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEELRYAHRAKLNIIIC
SKSLTNLAKKMQKTYGMPYLEESFYGMTDIAKALRNIAIALDDAVNGLEKRIMQDRVEQLLEEEEAKCRERLAPYRARLE
GKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHKDARIIEDTSTAGLLGVMKEKMPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWTLLNSPEPWETDATPLVNAARSHAATYLAEDLAASR
VAVPKKAATVNPQKNSPALGATLAYLGIDQMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEDSAIFGGWENLKKG
IGRVIEKFGPKVVGVMTSGLTETMGDDVRSAIHQFRVEHPEHDQVPIIWASTPDYCGSLQEGYANTVEAILATLPEAGEP
IPGQVNLVPGAQLTPADVEELKELIESFGLSVLCVPDIANALDGHIDADVSPLSTGGISVDKIRQAGRSIATLYVGDSLA
RAASQLEERLGIPAYGFTSLTGMAETDHFMSTLSAISGKAIPEKQQRWRSRLADALVDSHYQFGNKKVAIALEADHLKGM
TRFLAGMGCQIQAALAATRTRGLDSLPCNNVFVGDLEDLETAAVGADLLVANSNGRQAAAKLKIGAHLRTGLPVFDRLGA
HQKMWVGYRGTLNLVFEVANLFQANAKDAQKLAHN

Sequences:

>Translated_915_residues
MAKPDYYDVEDCSTHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDAIHLVHGPIACAGNSWDNRGARSSGSQLYR
RGFTTEMLENDVIFGGEKKLYKAIIDLAERYPEAKGMFVYATCVTAMTGDDIESVCKAAQEKVSIPLVPVNTPGFIGDKN
IGNRLAGEILFKQVIGTAEPPTLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEELRYAHRAKLNIIIC
SKSLTNLAKKMQKTYGMPYLEESFYGMTDIAKALRNIAIALDDAVNGLEKRIMQDRVEQLLEEEEAKCRERLAPYRARLE
GKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHKDARIIEDTSTAGLLGVMKEKMPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWTLLNSPEPWETDATPLVNAARSHAATYLAEDLAASR
VAVPKKAATVNPQKNSPALGATLAYLGIDQMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEDSAIFGGWENLKKG
IGRVIEKFGPKVVGVMTSGLTETMGDDVRSAIHQFRVEHPEHDQVPIIWASTPDYCGSLQEGYANTVEAILATLPEAGEP
IPGQVNLVPGAQLTPADVEELKELIESFGLSVLCVPDIANALDGHIDADVSPLSTGGISVDKIRQAGRSIATLYVGDSLA
RAASQLEERLGIPAYGFTSLTGMAETDHFMSTLSAISGKAIPEKQQRWRSRLADALVDSHYQFGNKKVAIALEADHLKGM
TRFLAGMGCQIQAALAATRTRGLDSLPCNNVFVGDLEDLETAAVGADLLVANSNGRQAAAKLKIGAHLRTGLPVFDRLGA
HQKMWVGYRGTLNLVFEVANLFQANAKDAQKLAHN
>Mature_914_residues
AKPDYYDVEDCSTHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDAIHLVHGPIACAGNSWDNRGARSSGSQLYRR
GFTTEMLENDVIFGGEKKLYKAIIDLAERYPEAKGMFVYATCVTAMTGDDIESVCKAAQEKVSIPLVPVNTPGFIGDKNI
GNRLAGEILFKQVIGTAEPPTLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEELRYAHRAKLNIIICS
KSLTNLAKKMQKTYGMPYLEESFYGMTDIAKALRNIAIALDDAVNGLEKRIMQDRVEQLLEEEEAKCRERLAPYRARLEG
KRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHKDARIIEDTSTAGLLGVMKEKMPDLIVAGGKTK
FLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWTLLNSPEPWETDATPLVNAARSHAATYLAEDLAASRV
AVPKKAATVNPQKNSPALGATLAYLGIDQMLGLLHGAQGCSTFIRLQLSRHFKESIALNATAMSEDSAIFGGWENLKKGI
GRVIEKFGPKVVGVMTSGLTETMGDDVRSAIHQFRVEHPEHDQVPIIWASTPDYCGSLQEGYANTVEAILATLPEAGEPI
PGQVNLVPGAQLTPADVEELKELIESFGLSVLCVPDIANALDGHIDADVSPLSTGGISVDKIRQAGRSIATLYVGDSLAR
AASQLEERLGIPAYGFTSLTGMAETDHFMSTLSAISGKAIPEKQQRWRSRLADALVDSHYQFGNKKVAIALEADHLKGMT
RFLAGMGCQIQAALAATRTRGLDSLPCNNVFVGDLEDLETAAVGADLLVANSNGRQAAAKLKIGAHLRTGLPVFDRLGAH
QKMWVGYRGTLNLVFEVANLFQANAKDAQKLAHN

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 99350; Mature: 99219

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPDYYDVEDCSTHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDAIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVIFGGEKKLYKAIIDLAERYPEAKGMFVY
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEE
ATCVTAMTGDDIESVCKAAQEKVSIPLVPVNTPGFIGDKNIGNRLAGEILFKQVIGTAEP
EEHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PTLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEELRYAHRAKLNIIIC
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEEE
SKSLTNLAKKMQKTYGMPYLEESFYGMTDIAKALRNIAIALDDAVNGLEKRIMQDRVEQL
CCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEEEAKCRERLAPYRARLEGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDF
HHHHHHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHHH
YRMKALMHKDARIIEDTSTAGLLGVMKEKMPDLIVAGGKTKFLALKTKTPFLDINHGRSH
HHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEECCCCCCC
PYAGYEGMVTFAKQLDLTVNNPIWTLLNSPEPWETDATPLVNAARSHAATYLAEDLAASR
CCCCCHHHHHHHHHHCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAVPKKAATVNPQKNSPALGATLAYLGIDQMLGLLHGAQGCSTFIRLQLSRHFKESIALN
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEC
ATAMSEDSAIFGGWENLKKGIGRVIEKFGPKVVGVMTSGLTETMGDDVRSAIHQFRVEHP
EEECCCCCCEECCHHHHHHHHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHCCCC
EHDQVPIIWASTPDYCGSLQEGYANTVEAILATLPEAGEPIPGQVNLVPGAQLTPADVEE
CCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHH
LKELIESFGLSVLCVPDIANALDGHIDADVSPLSTGGISVDKIRQAGRSIATLYVGDSLA
HHHHHHHHCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECHHHH
RAASQLEERLGIPAYGFTSLTGMAETDHFMSTLSAISGKAIPEKQQRWRSRLADALVDSH
HHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YQFGNKKVAIALEADHLKGMTRFLAGMGCQIQAALAATRTRGLDSLPCNNVFVGDLEDLE
HHCCCEEEEEEEEHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCEEECCHHHHH
TAAVGADLLVANSNGRQAAAKLKIGAHLRTGLPVFDRLGAHQKMWVGYRGTLNLVFEVAN
HHHCCCEEEEECCCCCHHHHHEEECCHHHCCCCHHHHCCCCCEEEEECCCHHHHHHHHHH
LFQANAKDAQKLAHN
HHHCCHHHHHHHHCC
>Mature Secondary Structure 
AKPDYYDVEDCSTHEKGAPKFCKKSEPGEGTERSCAYDGARVVLMPVTDAIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHCCCEE
CAGNSWDNRGARSSGSQLYRRGFTTEMLENDVIFGGEKKLYKAIIDLAERYPEAKGMFVY
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCCCEEEE
ATCVTAMTGDDIESVCKAAQEKVSIPLVPVNTPGFIGDKNIGNRLAGEILFKQVIGTAEP
EEHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PTLGEYPINLIGEYNIAGDLWGMLPLFDRLGIQILSCFSGDAKFEELRYAHRAKLNIIIC
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEEE
SKSLTNLAKKMQKTYGMPYLEESFYGMTDIAKALRNIAIALDDAVNGLEKRIMQDRVEQL
CCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEEEEAKCRERLAPYRARLEGKRAVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDF
HHHHHHHHHHHHCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHHH
YRMKALMHKDARIIEDTSTAGLLGVMKEKMPDLIVAGGKTKFLALKTKTPFLDINHGRSH
HHHHHHHHCCCHHCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEEECCCCEEECCCCCCC
PYAGYEGMVTFAKQLDLTVNNPIWTLLNSPEPWETDATPLVNAARSHAATYLAEDLAASR
CCCCCHHHHHHHHHHCEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAVPKKAATVNPQKNSPALGATLAYLGIDQMLGLLHGAQGCSTFIRLQLSRHFKESIALN
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEC
ATAMSEDSAIFGGWENLKKGIGRVIEKFGPKVVGVMTSGLTETMGDDVRSAIHQFRVEHP
EEECCCCCCEECCHHHHHHHHHHHHHHHCCEEEEHHHHCHHHHHHHHHHHHHHHHHCCCC
EHDQVPIIWASTPDYCGSLQEGYANTVEAILATLPEAGEPIPGQVNLVPGAQLTPADVEE
CCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHH
LKELIESFGLSVLCVPDIANALDGHIDADVSPLSTGGISVDKIRQAGRSIATLYVGDSLA
HHHHHHHHCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECHHHH
RAASQLEERLGIPAYGFTSLTGMAETDHFMSTLSAISGKAIPEKQQRWRSRLADALVDSH
HHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YQFGNKKVAIALEADHLKGMTRFLAGMGCQIQAALAATRTRGLDSLPCNNVFVGDLEDLE
HHCCCEEEEEEEEHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCCCEEECCHHHHH
TAAVGADLLVANSNGRQAAAKLKIGAHLRTGLPVFDRLGAHQKMWVGYRGTLNLVFEVAN
HHHCCCEEEEECCCCCHHHHHEEECCHHHCCCCHHHHCCCCCEEEEECCCHHHHHHHHHH
LFQANAKDAQKLAHN
HHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]