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The map label for this gene is dosC [H]

Identifier: 189423671

GI number: 189423671

Start: 594171

End: 595640

Strand: Reverse

Name: dosC [H]

Synonym: Glov_0601

Alternate gene names: 189423671

Gene position: 595640-594171 (Counterclockwise)

Preceding gene: 189423679

Following gene: 189423670

Centisome position: 15.2

GC content: 59.32

Gene sequence:

>1470_bases
ATGTACAAATACAGCAACAAGATCGATGCACTGACCGCCATATTAAAAAGACTGGATACCGGGGAAAAGCTGACGCCGGA
GCTGCTGGCCGGGGATTTCCATGTCGGGGTGCGCACCATCTACCGCTACCTGAACCATCTCCAGGCCGCCGGCTATCCGA
TCTACTTTGACAAGGCCGACAGGAGCTACCGTTTCCTGAACAACTTCAGGCTGAACCAGCTCAAGTCAGGCGGCGGCGAC
GAGGCGTTCAGCCTGCACCCGATCAACCAGATGAACGGCGTGGCCATTGCCACCTTCCGTACCAGCGGTGAATGTATCCA
TAAGAATACGGCCATGACCCGCCTGACAGGGTGCGCCAGCTGGAACTGCTGCAGCGGTAATTTCCGGCAGCTGGACTGGT
GGCAGGATTCAGGCCTGCTTGCCATGGCCGATGAGGCGATTGAGACGGGACGGGAAGTCTGCCGTGACATTACGCTGACC
GTCAACAAACGGGAACGCTGGATCCAGGCCCACATGACCCTGGTGAAACAGGAGAAGACCGCCTATCTGGTGTTCCTGGC
CCAGGATCTCTCGCCCCGCATGCACAAGGAGATCCAGGTCGCCCGCTTCTTTGCCGCCATGAATCAGTCACCCAACCTGA
TTCTGGTTACCGATACCCGGGGCACGATTGAATATGTCAGCGAACGGGTGGAAGAGCTGACCGGCTATACCGCCGAGGAA
CTGATCGGCGCCAATCCCCGCATCCTCAAGTCGGGTCAGACCCCGCCCGAGGTGTATCAGAACCTCTGGAGCACCATCAG
CCGCGGCTATGCCTGGAGCGGTGAGCTGTACAACCGCAAGAAAAACGGCGACTACTACTGGCAGCACCTCCTCATTGCAC
CGATCCGTAATCATCACAACGGCATCTCCCGTTATGTGGCGGTGATCGAGGATATCTCCCGCCAGAAGCTGCTGGAGGAG
GAGATCTACGCCTATGCCGTCAGCGACCGGGTCACCGGCCTCTACAACCGCAAGATACTGCTTGAACTGGGGAACCGCGA
CATCACGGCGGCACAGCGCTACGGCCGTGCCATGACCCTTCTGATCGTGGATATCGATCAGTTCAAGGAGATTAACGATC
AGTTCGGCTACCCGGCCGGCAACCAGTTCATGCAGCAACTGGCGGCCCTCTGCCGCGCATCGCTACGGACCACCGACCTG
ATCGGCCGGACCGGCAAGGACTCCTTCGGCATTCTGCTGGCTGAATCCGCGCTTGCCGACGCCCTGCTGGTTGCCGAGCG
GATCCGGGAGAAAGCGGCACAGATCAGGATTACGACGGCTGAAGGGGAGTTGGCCTGCACCGTCAGCGTCGGCGGCGTAG
CACTGAACAACCGGCATCAGACCATGGAACAACTGGTGATCGAGTGCGAAGGGTTGCTGCAGGGCAGCCAGTCGCAGCGG
ACCGTTAACCAGTGCATCGGTTTTGCATGA

Upstream 100 bases:

>100_bases
GCTAGACTAAAGGGAAACAAAAAGACAAATAATACCAACAATATCCTGTTTTGCTTCAGACCTGTCAAATGCACCCACGC
AAAGGACCTCCAGCTTTTTT

Downstream 100 bases:

>100_bases
CGCCGGGCAGCAGCGTGGATAGGCAGCTCAGCCAACGAACCGGCCAGCACTGGCTTGCGCTGATCGTCGCCCTGGCCCTG
ATCGGCAGTATCATCGCCTA

Product: PAS/PAC sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MYKYSNKIDALTAILKRLDTGEKLTPELLAGDFHVGVRTIYRYLNHLQAAGYPIYFDKADRSYRFLNNFRLNQLKSGGGD
EAFSLHPINQMNGVAIATFRTSGECIHKNTAMTRLTGCASWNCCSGNFRQLDWWQDSGLLAMADEAIETGREVCRDITLT
VNKRERWIQAHMTLVKQEKTAYLVFLAQDLSPRMHKEIQVARFFAAMNQSPNLILVTDTRGTIEYVSERVEELTGYTAEE
LIGANPRILKSGQTPPEVYQNLWSTISRGYAWSGELYNRKKNGDYYWQHLLIAPIRNHHNGISRYVAVIEDISRQKLLEE
EIYAYAVSDRVTGLYNRKILLELGNRDITAAQRYGRAMTLLIVDIDQFKEINDQFGYPAGNQFMQQLAALCRASLRTTDL
IGRTGKDSFGILLAESALADALLVAERIREKAAQIRITTAEGELACTVSVGGVALNNRHQTMEQLVIECEGLLQGSQSQR
TVNQCIGFA

Sequences:

>Translated_489_residues
MYKYSNKIDALTAILKRLDTGEKLTPELLAGDFHVGVRTIYRYLNHLQAAGYPIYFDKADRSYRFLNNFRLNQLKSGGGD
EAFSLHPINQMNGVAIATFRTSGECIHKNTAMTRLTGCASWNCCSGNFRQLDWWQDSGLLAMADEAIETGREVCRDITLT
VNKRERWIQAHMTLVKQEKTAYLVFLAQDLSPRMHKEIQVARFFAAMNQSPNLILVTDTRGTIEYVSERVEELTGYTAEE
LIGANPRILKSGQTPPEVYQNLWSTISRGYAWSGELYNRKKNGDYYWQHLLIAPIRNHHNGISRYVAVIEDISRQKLLEE
EIYAYAVSDRVTGLYNRKILLELGNRDITAAQRYGRAMTLLIVDIDQFKEINDQFGYPAGNQFMQQLAALCRASLRTTDL
IGRTGKDSFGILLAESALADALLVAERIREKAAQIRITTAEGELACTVSVGGVALNNRHQTMEQLVIECEGLLQGSQSQR
TVNQCIGFA
>Mature_489_residues
MYKYSNKIDALTAILKRLDTGEKLTPELLAGDFHVGVRTIYRYLNHLQAAGYPIYFDKADRSYRFLNNFRLNQLKSGGGD
EAFSLHPINQMNGVAIATFRTSGECIHKNTAMTRLTGCASWNCCSGNFRQLDWWQDSGLLAMADEAIETGREVCRDITLT
VNKRERWIQAHMTLVKQEKTAYLVFLAQDLSPRMHKEIQVARFFAAMNQSPNLILVTDTRGTIEYVSERVEELTGYTAEE
LIGANPRILKSGQTPPEVYQNLWSTISRGYAWSGELYNRKKNGDYYWQHLLIAPIRNHHNGISRYVAVIEDISRQKLLEE
EIYAYAVSDRVTGLYNRKILLELGNRDITAAQRYGRAMTLLIVDIDQFKEINDQFGYPAGNQFMQQLAALCRASLRTTDL
IGRTGKDSFGILLAESALADALLVAERIREKAAQIRITTAEGELACTVSVGGVALNNRHQTMEQLVIECEGLLQGSQSQR
TVNQCIGFA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=35.0993377483444, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=285, Percent_Identity=26.6666666666667, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=33.5365853658537, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=151, Percent_Identity=34.4370860927152, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786584, Length=128, Percent_Identity=32.03125, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=29.8780487804878, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=285, Percent_Identity=22.8070175438596, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081974, Length=109, Percent_Identity=33.9449541284404, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789453, Length=112, Percent_Identity=30.3571428571429, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788085, Length=156, Percent_Identity=25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55220; Mature: 55220

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKYSNKIDALTAILKRLDTGEKLTPELLAGDFHVGVRTIYRYLNHLQAAGYPIYFDKAD
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
RSYRFLNNFRLNQLKSGGGDEAFSLHPINQMNGVAIATFRTSGECIHKNTAMTRLTGCAS
CCHHHHHHCCHHHHCCCCCCCCEEECCCCCCCCEEEEEEECCCCCEECCCHHHHHHCCCC
WNCCSGNFRQLDWWQDSGLLAMADEAIETGREVCRDITLTVNKRERWIQAHMTLVKQEKT
CCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCC
AYLVFLAQDLSPRMHKEIQVARFFAAMNQSPNLILVTDTRGTIEYVSERVEELTGYTAEE
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHH
LIGANPRILKSGQTPPEVYQNLWSTISRGYAWSGELYNRKKNGDYYWQHLLIAPIRNHHN
HHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCH
GISRYVAVIEDISRQKLLEEEIYAYAVSDRVTGLYNRKILLELGNRDITAAQRYGRAMTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCEEEE
LIVDIDQFKEINDQFGYPAGNQFMQQLAALCRASLRTTDLIGRTGKDSFGILLAESALAD
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEHHHHHH
ALLVAERIREKAAQIRITTAEGELACTVSVGGVALNNRHQTMEQLVIECEGLLQGSQSQR
HHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCCHHH
TVNQCIGFA
HHHHHCCCC
>Mature Secondary Structure
MYKYSNKIDALTAILKRLDTGEKLTPELLAGDFHVGVRTIYRYLNHLQAAGYPIYFDKAD
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCC
RSYRFLNNFRLNQLKSGGGDEAFSLHPINQMNGVAIATFRTSGECIHKNTAMTRLTGCAS
CCHHHHHHCCHHHHCCCCCCCCEEECCCCCCCCEEEEEEECCCCCEECCCHHHHHHCCCC
WNCCSGNFRQLDWWQDSGLLAMADEAIETGREVCRDITLTVNKRERWIQAHMTLVKQEKT
CCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHCCCC
AYLVFLAQDLSPRMHKEIQVARFFAAMNQSPNLILVTDTRGTIEYVSERVEELTGYTAEE
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHH
LIGANPRILKSGQTPPEVYQNLWSTISRGYAWSGELYNRKKNGDYYWQHLLIAPIRNHHN
HHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCH
GISRYVAVIEDISRQKLLEEEIYAYAVSDRVTGLYNRKILLELGNRDITAAQRYGRAMTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCEEEE
LIVDIDQFKEINDQFGYPAGNQFMQQLAALCRASLRTTDLIGRTGKDSFGILLAESALAD
EEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEHHHHHH
ALLVAERIREKAAQIRITTAEGELACTVSVGGVALNNRHQTMEQLVIECEGLLQGSQSQR
HHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEECCHHHHHHHHHHHHHHHHCCCCHHH
TVNQCIGFA
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]