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The map label for this gene is amtB [C]

Identifier: 189423542

GI number: 189423542

Start: 446215

End: 447705

Strand: Reverse

Name: amtB [C]

Synonym: Glov_0471

Alternate gene names: 189423542

Gene position: 447705-446215 (Counterclockwise)

Preceding gene: 189423543

Following gene: 189423541

Centisome position: 11.43

GC content: 57.95

Gene sequence:

>1491_bases
ATGAAACTCAAGCATTCACTCATTGTTGTCATGTTGACCCTCTTCACCGCACTGGTAGGCGTTTCTGCCATGGCTGAAGA
GAAAAAAGATGCGGCGCCTGCTGCTGCAGTTGCAACTGAGGCGCCCAAGGCACCTGAAGCGGCAGCTCCCGCAGCTGCAC
CGGTTGCTGCTCCGGCCGCTGCCGCTCCGGCGGCCCTGCCAGACGTCAAACCGGTTCTGAACACTGGTGATACCGCCTGG
ATGCTGGTCTCATCAGCCCTGGTACTGTTGATGATCCCCGGTCTGGCCCTGTTTTACGGTGGTATGGTCCGCCAGAAAAA
CGTCCTGTCCACCATGATGCACTCCTTTGTTGCCATGGGTATTGTCGGGGTACAATGGGCAGTAATCGGCTACTCACTCG
CCTTTGCACCTGATATGGGCGGCATGGGCCTGATCGGCAACTTCAGCAAATTCATGCTGAACGGCCTGATCGTGATGAAG
GACGCTGCATCCGGTACGGTGGAGTGGACCCTGTTCCAGAACTACACCAAAGAGCCCGGCGCAATCCCGGAGCTGGTCTT
TGCCATGTATCAGGCGATGTTTGCCATGATTACCGTGGCCCTGATTTCAGGTGCCATGGCTGAGCGGGTCAAATTCTCCG
CCTACTGCCTGTTTGTACTGCTCTGGACCACCCTTGTCTATGATCCCCTGGCACACTGGGTCTGGATGACTGAAGGCTGG
TTGTTCAAAAAAGGCGCACTGGACTTTGCCGGCGGCACCGTTGTTCACCTCTCCTCCGGTATCTCCGCTCTGGTATTTCT
GGCCTTCCTCGGTAAGCGTCACGGTTTCCCCAACGAGCGGATGGCGCCCCACAACCTGCCGTTGACCCTGCTTGGTGTCG
GTCTGCTCTGGTTCGGTTGGTACGGTTTCAACGCAGGTTCGGCAATCGTGGGTGTCAACAACTCTGATGCAGCAGGCGGC
CTGGCCGGTCTGGCCTTTGCCACCACCACCATTGCCCCTGCAGCAGCCGGTCTTTCCTGGATGATCGCTGAGTGGATCCA
CTCCGGCAAGCCTTCTGCCCTGGGTTTTGGCTCAGGTGTTGTTGCCGGCCTGGTTGTGATCACCCCTGCCGCCGGTTTCG
TACAACCGGGCGCCGCCCTGATCATGGGTGTGGCTGCAGGCCTGGTCTGCTACGGCGGCATCCTGCTCAAGGCCAAACTG
AAGTATGACGACTCCCTTGACGCCTTTGGTGTTCACGGTATCGGCGGCACCTTCGGCGCCATTATCACCGGCGTATTTGC
CACCGTAGGCGCAACCGGCCTGCTGGCCGGCAATGCCAAGCAGCTGATGATTCAGCTGATTGCGGTACTGGCAGCCGGTG
CCTACGCAGTGGTCGTAACCCTGATCATCACCTTCATCCTGGACAAGACCATTGGTCTGCGGGTAGAGAAGGAAGATGAA
ATCATGGGTCTTGACCAGACACAACACAGCGAATCAGGCTACAACATGTAA

Upstream 100 bases:

>100_bases
CGCCTTGGAAGAAGCAGTACGGATCCGTACCGGCGAAACCGGTGGCGAAGCAATCTAAACACAACTCAAGAAGCCATTTT
AAATAGTCAGGAGGCTGGTT

Downstream 100 bases:

>100_bases
CAGCCGTGCCGGTGCAGCAGTAAACGGGCCGGTCATCACACGGGGTGATGACCGGCCCGGATTGTTTTTTAGCGGAAGCG
CACAAAAAAAAGCTTGCATT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MKLKHSLIVVMLTLFTALVGVSAMAEEKKDAAPAAAVATEAPKAPEAAAPAAAPVAAPAAAAPAALPDVKPVLNTGDTAW
MLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSFVAMGIVGVQWAVIGYSLAFAPDMGGMGLIGNFSKFMLNGLIVMK
DAASGTVEWTLFQNYTKEPGAIPELVFAMYQAMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDPLAHWVWMTEGW
LFKKGALDFAGGTVVHLSSGISALVFLAFLGKRHGFPNERMAPHNLPLTLLGVGLLWFGWYGFNAGSAIVGVNNSDAAGG
LAGLAFATTTIAPAAAGLSWMIAEWIHSGKPSALGFGSGVVAGLVVITPAAGFVQPGAALIMGVAAGLVCYGGILLKAKL
KYDDSLDAFGVHGIGGTFGAIITGVFATVGATGLLAGNAKQLMIQLIAVLAAGAYAVVVTLIITFILDKTIGLRVEKEDE
IMGLDQTQHSESGYNM

Sequences:

>Translated_496_residues
MKLKHSLIVVMLTLFTALVGVSAMAEEKKDAAPAAAVATEAPKAPEAAAPAAAPVAAPAAAAPAALPDVKPVLNTGDTAW
MLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSFVAMGIVGVQWAVIGYSLAFAPDMGGMGLIGNFSKFMLNGLIVMK
DAASGTVEWTLFQNYTKEPGAIPELVFAMYQAMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDPLAHWVWMTEGW
LFKKGALDFAGGTVVHLSSGISALVFLAFLGKRHGFPNERMAPHNLPLTLLGVGLLWFGWYGFNAGSAIVGVNNSDAAGG
LAGLAFATTTIAPAAAGLSWMIAEWIHSGKPSALGFGSGVVAGLVVITPAAGFVQPGAALIMGVAAGLVCYGGILLKAKL
KYDDSLDAFGVHGIGGTFGAIITGVFATVGATGLLAGNAKQLMIQLIAVLAAGAYAVVVTLIITFILDKTIGLRVEKEDE
IMGLDQTQHSESGYNM
>Mature_496_residues
MKLKHSLIVVMLTLFTALVGVSAMAEEKKDAAPAAAVATEAPKAPEAAAPAAAPVAAPAAAAPAALPDVKPVLNTGDTAW
MLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSFVAMGIVGVQWAVIGYSLAFAPDMGGMGLIGNFSKFMLNGLIVMK
DAASGTVEWTLFQNYTKEPGAIPELVFAMYQAMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDPLAHWVWMTEGW
LFKKGALDFAGGTVVHLSSGISALVFLAFLGKRHGFPNERMAPHNLPLTLLGVGLLWFGWYGFNAGSAIVGVNNSDAAGG
LAGLAFATTTIAPAAAGLSWMIAEWIHSGKPSALGFGSGVVAGLVVITPAAGFVQPGAALIMGVAAGLVCYGGILLKAKL
KYDDSLDAFGVHGIGGTFGAIITGVFATVGATGLLAGNAKQLMIQLIAVLAAGAYAVVVTLIITFILDKTIGLRVEKEDE
IMGLDQTQHSESGYNM

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=447, Percent_Identity=43.8478747203579, Blast_Score=316, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17550182, Length=458, Percent_Identity=31.4410480349345, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17550180, Length=449, Percent_Identity=30.9576837416481, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=444, Percent_Identity=26.5765765765766, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=446, Percent_Identity=27.1300448430493, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=36.9469026548673, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6325396, Length=434, Percent_Identity=33.8709677419355, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321559, Length=433, Percent_Identity=32.1016166281755, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161078314, Length=451, Percent_Identity=31.2638580931264, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51463; Mature: 51463

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKHSLIVVMLTLFTALVGVSAMAEEKKDAAPAAAVATEAPKAPEAAAPAAAPVAAPAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
AAPAALPDVKPVLNTGDTAWMLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSFVAMG
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IVGVQWAVIGYSLAFAPDMGGMGLIGNFSKFMLNGLIVMKDAASGTVEWTLFQNYTKEPG
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCC
AIPELVFAMYQAMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDPLAHWVWMTEGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LFKKGALDFAGGTVVHLSSGISALVFLAFLGKRHGFPNERMAPHNLPLTLLGVGLLWFGW
EEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
YGFNAGSAIVGVNNSDAAGGLAGLAFATTTIAPAAAGLSWMIAEWIHSGKPSALGFGSGV
HCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAGLVVITPAAGFVQPGAALIMGVAAGLVCYGGILLKAKLKYDDSLDAFGVHGIGGTFGA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHH
IITGVFATVGATGLLAGNAKQLMIQLIAVLAAGAYAVVVTLIITFILDKTIGLRVEKEDE
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEECCCCC
IMGLDQTQHSESGYNM
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLKHSLIVVMLTLFTALVGVSAMAEEKKDAAPAAAVATEAPKAPEAAAPAAAPVAAPAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
AAPAALPDVKPVLNTGDTAWMLVSSALVLLMIPGLALFYGGMVRQKNVLSTMMHSFVAMG
CCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IVGVQWAVIGYSLAFAPDMGGMGLIGNFSKFMLNGLIVMKDAASGTVEWTLFQNYTKEPG
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCCC
AIPELVFAMYQAMFAMITVALISGAMAERVKFSAYCLFVLLWTTLVYDPLAHWVWMTEGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LFKKGALDFAGGTVVHLSSGISALVFLAFLGKRHGFPNERMAPHNLPLTLLGVGLLWFGW
EEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
YGFNAGSAIVGVNNSDAAGGLAGLAFATTTIAPAAAGLSWMIAEWIHSGKPSALGFGSGV
HCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VAGLVVITPAAGFVQPGAALIMGVAAGLVCYGGILLKAKLKYDDSLDAFGVHGIGGTFGA
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHH
IITGVFATVGATGLLAGNAKQLMIQLIAVLAAGAYAVVVTLIITFILDKTIGLRVEKEDE
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEECCCCC
IMGLDQTQHSESGYNM
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]