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The map label for this gene is pleD [H]

Identifier: 189354100

GI number: 189354100

Start: 2460278

End: 2461216

Strand: Reverse

Name: pleD [H]

Synonym: BMULJ_05354

Alternate gene names: 189354100

Gene position: 2461216-2460278 (Counterclockwise)

Preceding gene: 189354101

Following gene: 189354098

Centisome position: 99.52

GC content: 68.9

Gene sequence:

>939_bases
ATGACGGCCGAACGCGCGTCGCTCAGCGATCTCGTGATCGAGCGCGTCGGCTTCGGGCTGTTCGTGCTCGATCGCCGCAT
GACCGTGCTGATGTGGAATCGCTTCATGCAGGACCACAGCGGCGTGCCGGCCGCCGACGTGATCGGCCGCAACCTGTTCG
ACTGCTTCCCCGACCTGCCGCGCGCATGGCTCACACGCAAGCTCGAGAGCGTGTTCCAGCTCGGCAGCTTCGCGTTCAGC
TCGTGGGAGCAGCGGCCCTACCTGTTTCGTTTCGAGCACGATCGGCCGATCACGGGCGGCGTCGACTACATGCAGCAGGA
CTGCACGTTCATGCCGCTCACGCGCGGCCGCGACGTCGAAGCGGTGTGCGTCACGATCTCGGACGTCACGCACGTCAGCG
TGATGCAGCGCGAGCGCGAAGAGGCGGTCGCGAAGCTGCGCGAGCATGCGAACCGCGACGGCCTCACCGGCATCGCCAAT
CGGCGCTACTTCGAGACGCGGCTCGGCGAAGAATTCGCGCGCTGGCAGCGCTACGGCGGCGATCTGTCGATGCTGCTGTT
CGATCTCGACCACTTCAAGGCGATCAACGACCGCTTCGGCCATGCGGTCGGCGATGCGGTGCTGCGCGAGACCGCGCGCC
GCGTGGCGGCGATCGTGCGCGCGCAGGATACGTTCGGCCGCTTCGGCGGCGAGGAATTCGCGCTGCTGCTGCCGTGCACG
AATCTCGAGGAAGCGCTGCAGGTCGCGGACAAGGTGCGCGACGCGATCGGCCGCACGCCGGTGGACGCGGAAGGCGTCAG
CGTCGCGGTGACGGCGAGCGTCGGCGCCGCGTGCGCGAAGCTCGGACAGCCCGCGTGCGACGCGCTCGTCAACGACGCGG
ACGCGGCGCTCTATCGCGCGAAACGGCTCGGCCGCGACCGCTCGGTCGGCTACGCGTAG

Upstream 100 bases:

>100_bases
GTCTCGAGGATCACGCGTTTCGCGCACATTTCGTGATGCTGATGGCCGAGGACTCGATCCGGCTGATGGGCGACGCGCTC
GACGCGTTGCTGTCCGCGCT

Downstream 100 bases:

>100_bases
GCGGCGCGGTCGATCAGTCGGTCACTCGGTCAGTCAGTGCTTCGCGATGTCGGCCAGCACGCCGGCGAGCACGGCCGGCT
GCGACATGAACGGCGAATGG

Product: putative diguanylate cyclase protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTAERASLSDLVIERVGFGLFVLDRRMTVLMWNRFMQDHSGVPAADVIGRNLFDCFPDLPRAWLTRKLESVFQLGSFAFS
SWEQRPYLFRFEHDRPITGGVDYMQQDCTFMPLTRGRDVEAVCVTISDVTHVSVMQREREEAVAKLREHANRDGLTGIAN
RRYFETRLGEEFARWQRYGGDLSMLLFDLDHFKAINDRFGHAVGDAVLRETARRVAAIVRAQDTFGRFGGEEFALLLPCT
NLEEALQVADKVRDAIGRTPVDAEGVSVAVTASVGAACAKLGQPACDALVNDADAALYRAKRLGRDRSVGYA

Sequences:

>Translated_312_residues
MTAERASLSDLVIERVGFGLFVLDRRMTVLMWNRFMQDHSGVPAADVIGRNLFDCFPDLPRAWLTRKLESVFQLGSFAFS
SWEQRPYLFRFEHDRPITGGVDYMQQDCTFMPLTRGRDVEAVCVTISDVTHVSVMQREREEAVAKLREHANRDGLTGIAN
RRYFETRLGEEFARWQRYGGDLSMLLFDLDHFKAINDRFGHAVGDAVLRETARRVAAIVRAQDTFGRFGGEEFALLLPCT
NLEEALQVADKVRDAIGRTPVDAEGVSVAVTASVGAACAKLGQPACDALVNDADAALYRAKRLGRDRSVGYA
>Mature_311_residues
TAERASLSDLVIERVGFGLFVLDRRMTVLMWNRFMQDHSGVPAADVIGRNLFDCFPDLPRAWLTRKLESVFQLGSFAFSS
WEQRPYLFRFEHDRPITGGVDYMQQDCTFMPLTRGRDVEAVCVTISDVTHVSVMQREREEAVAKLREHANRDGLTGIANR
RYFETRLGEEFARWQRYGGDLSMLLFDLDHFKAINDRFGHAVGDAVLRETARRVAAIVRAQDTFGRFGGEEFALLLPCTN
LEEALQVADKVRDAIGRTPVDAEGVSVAVTASVGAACAKLGQPACDALVNDADAALYRAKRLGRDRSVGYA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=36.7231638418079, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=37.7777777777778, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=196, Percent_Identity=36.2244897959184, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788381, Length=214, Percent_Identity=33.6448598130841, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=33.6842105263158, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=37.3493975903614, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787541, Length=306, Percent_Identity=25.4901960784314, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787802, Length=163, Percent_Identity=31.9018404907975, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=30.625, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788085, Length=207, Percent_Identity=27.0531400966184, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=169, Percent_Identity=30.1775147928994, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787056, Length=171, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34933; Mature: 34802

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50112 PAS ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAERASLSDLVIERVGFGLFVLDRRMTVLMWNRFMQDHSGVPAADVIGRNLFDCFPDLP
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCH
RAWLTRKLESVFQLGSFAFSSWEQRPYLFRFEHDRPITGGVDYMQQDCTFMPLTRGRDVE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCEECCCCCCCCE
AVCVTISDVTHVSVMQREREEAVAKLREHANRDGLTGIANRRYFETRLGEEFARWQRYGG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCC
DLSMLLFDLDHFKAINDRFGHAVGDAVLRETARRVAAIVRAQDTFGRFGGEEFALLLPCT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
NLEEALQVADKVRDAIGRTPVDAEGVSVAVTASVGAACAKLGQPACDALVNDADAALYRA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCHHHHHHHHH
KRLGRDRSVGYA
HHCCCCCCCCCC
>Mature Secondary Structure 
TAERASLSDLVIERVGFGLFVLDRRMTVLMWNRFMQDHSGVPAADVIGRNLFDCFPDLP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCH
RAWLTRKLESVFQLGSFAFSSWEQRPYLFRFEHDRPITGGVDYMQQDCTFMPLTRGRDVE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCEECCCCCCCCE
AVCVTISDVTHVSVMQREREEAVAKLREHANRDGLTGIANRRYFETRLGEEFARWQRYGG
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCC
DLSMLLFDLDHFKAINDRFGHAVGDAVLRETARRVAAIVRAQDTFGRFGGEEFALLLPCT
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
NLEEALQVADKVRDAIGRTPVDAEGVSVAVTASVGAACAKLGQPACDALVNDADAALYRA
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHCCHHHHHHHCHHHHHHHHH
KRLGRDRSVGYA
HHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA