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The map label for this gene is paaG [C]

Identifier: 189354062

GI number: 189354062

Start: 2394260

End: 2395087

Strand: Reverse

Name: paaG [C]

Synonym: BMULJ_05315

Alternate gene names: 189354062

Gene position: 2395087-2394260 (Counterclockwise)

Preceding gene: 189354063

Following gene: 189354061

Centisome position: 96.84

GC content: 64.37

Gene sequence:

>828_bases
ATGAGTTACGAAGGTCGCTGGAAAACCGTGAAAGTCGCCGTCGAGGGCGGGATTGCGTGGGTGACGCTGAATCGTCCCGA
GAAGCGCAACGCGATGAGCCCGACGCTGAACGCGGAAATGATCGACGTGCTCGAAGCGATCGAACTCGACCCCGAAGCGC
AGGTGCTCGTGCTGACCGGCGAAGGCGATGCATGGACGGCCGGGATGGATCTCAAGGAATATTTCCGCGAAGTCGATGCG
GGCCCCGAAATCCTGCAGGAAAAGATCCGCCGCGACGCGAGCCGCTGGCAGTGGCAGCTGCTGCGCATGTACGCGAAGCC
GACGATCGCGATGGTCAACGGCTGGTGCTTCGGCGGCGGCTTCTCGCCGCTCGTCGCCTGCGATCTCGCGATCGCGGCCG
ACGAGGCGGTGTTCGGGCTGTCCGAGATCAACTGGGGCATTCCGCCGGGCAATCTGGTCAGCAAGGCGATGGCCGATACG
GTCGGTCATCGTCAGGCGCTCTACTACATCATGACGGGCGACACGTTCACGGGCCAGCAGGCCGCGCAGATGGGCCTCGT
CAACAAGAGCGTGCCGCGCGCGCAGCTGCGCGACGAGGTGCGTGCGCTCGCCGCGAAGCTGCTCGACAAGAACCCGGTCG
TGATCCGCAACGCGAAGCACGGCTTCAAGCGGTGCCGCGAACTGACGTGGGAGCAGAACGAGGACTACCTGTACGCGAAG
CTCGACCAGGCCAACTATCGCGATCCGGAAGGCGGCCGCGAGCAGGGTCTCAAGCAGTTCCTCGACGAAAAGAGCATCAA
GCCGGGCCTGCAGGCGTACAAGCGCTGA

Upstream 100 bases:

>100_bases
GTTTGTCCGGACGCGCGCCCAATCCGCTTTACATATCAGGCTACCTGATAGTAAATTGGCGGCGAGTCGCAACGGGCGGC
ACTGTCATTGGAGAACGAAG

Downstream 100 bases:

>100_bases
GCGGCGACGCGGCGCAACCCGACCTGGGCCGAAACTGAGCGGAGACAGCGAAATGCACGAAGTGAGTTTGTTGATCGACG
GCGTGTCGCGCGGCGCGTCG

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSYEGRWKTVKVAVEGGIAWVTLNRPEKRNAMSPTLNAEMIDVLEAIELDPEAQVLVLTGEGDAWTAGMDLKEYFREVDA
GPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGDTFTGQQAAQMGLVNKSVPRAQLRDEVRALAAKLLDKNPVVIRNAKHGFKRCRELTWEQNEDYLYAK
LDQANYRDPEGGREQGLKQFLDEKSIKPGLQAYKR

Sequences:

>Translated_275_residues
MSYEGRWKTVKVAVEGGIAWVTLNRPEKRNAMSPTLNAEMIDVLEAIELDPEAQVLVLTGEGDAWTAGMDLKEYFREVDA
GPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADT
VGHRQALYYIMTGDTFTGQQAAQMGLVNKSVPRAQLRDEVRALAAKLLDKNPVVIRNAKHGFKRCRELTWEQNEDYLYAK
LDQANYRDPEGGREQGLKQFLDEKSIKPGLQAYKR
>Mature_274_residues
SYEGRWKTVKVAVEGGIAWVTLNRPEKRNAMSPTLNAEMIDVLEAIELDPEAQVLVLTGEGDAWTAGMDLKEYFREVDAG
PEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGGFSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTV
GHRQALYYIMTGDTFTGQQAAQMGLVNKSVPRAQLRDEVRALAAKLLDKNPVVIRNAKHGFKRCRELTWEQNEDYLYAKL
DQANYRDPEGGREQGLKQFLDEKSIKPGLQAYKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=30.6772908366534, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=28, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=29.5719844357977, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI45643119, Length=201, Percent_Identity=29.3532338308458, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=201, Percent_Identity=29.3532338308458, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI20127408, Length=188, Percent_Identity=28.7234042553192, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=31.0756972111554, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788597, Length=209, Percent_Identity=35.8851674641148, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI221142681, Length=238, Percent_Identity=30.672268907563, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=23.4200743494424, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=27.8947368421053, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787660, Length=198, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=29.8850574712644, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=29.2, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=27.7310924369748, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=205, Percent_Identity=29.2682926829268, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=234, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=205, Percent_Identity=27.3170731707317, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=24.8677248677249, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=273, Percent_Identity=28.2051282051282, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653477, Length=273, Percent_Identity=28.2051282051282, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357171, Length=185, Percent_Identity=28.6486486486486, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=28.90625, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922422, Length=270, Percent_Identity=24.0740740740741, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30867; Mature: 30735

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEGRWKTVKVAVEGGIAWVTLNRPEKRNAMSPTLNAEMIDVLEAIELDPEAQVLVLTG
CCCCCCEEEEEEEEECCEEEEEECCCHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGDAWTAGMDLKEYFREVDAGPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGG
CCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCC
FSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGDTFTGQQ
CCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNKSVPRAQLRDEVRALAAKLLDKNPVVIRNAKHGFKRCRELTWEQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEE
LDQANYRDPEGGREQGLKQFLDEKSIKPGLQAYKR
ECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure 
SYEGRWKTVKVAVEGGIAWVTLNRPEKRNAMSPTLNAEMIDVLEAIELDPEAQVLVLTG
CCCCCEEEEEEEEECCEEEEEECCCHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
EGDAWTAGMDLKEYFREVDAGPEILQEKIRRDASRWQWQLLRMYAKPTIAMVNGWCFGGG
CCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEECCC
FSPLVACDLAIAADEAVFGLSEINWGIPPGNLVSKAMADTVGHRQALYYIMTGDTFTGQQ
CCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHH
AAQMGLVNKSVPRAQLRDEVRALAAKLLDKNPVVIRNAKHGFKRCRELTWEQNEDYLYAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCCCCEEEEE
LDQANYRDPEGGREQGLKQFLDEKSIKPGLQAYKR
ECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA