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The map label for this gene is oppA [H]

Identifier: 189354009

GI number: 189354009

Start: 2336892

End: 2338505

Strand: Reverse

Name: oppA [H]

Synonym: BMULJ_05262

Alternate gene names: 189354009

Gene position: 2338505-2336892 (Counterclockwise)

Preceding gene: 189354010

Following gene: 189354008

Centisome position: 94.56

GC content: 64.25

Gene sequence:

>1614_bases
ATGAAATCCTTGCATGCCATGTCGGCCGTGCTGGCCGCGCTCGTCCTGACGACGCCCGCCGCCCGTGCCGTAACCGTCCC
GTCGAACGTCACGCTTGCCGCGCAGCAGGATCTGACGCGACAAGTGCCCGCCGAAGTCGAGTCGCTCGATCCCGCCCACA
TCGAATCGTGGACCGGCAACACCATCGGCCTCGACCTGTTCGAAGGGCTCGCGCGCATCGACGCGGCCGGGCAGGTCGTG
CCGGGCGTCGCGCTGTCGTGGGAGCGCAAGACGCCGCAGACGTGGATCTTCAAGCTGCGTCACGACGCGAAGTGGAGCAA
CGGGCAGCCCGTGACGGCCGCCGATTTCGTCTATTCGTGGCAGCGCCTCGCCGATCCGAAGACGGGCTCGAAATACACGA
TCCTCGTCGAGTTCGTGAAGAACTCGAAGGACATCATCGCGGGCAAGGCCGCGCCGTCGACGCTCGGCGTGCGCGCGGTC
GATCCGTACACGCTCGAGGTGACGACCGACGTGCCGGTCGCGTTCTTCCCCGAGCTGACCGCGATGGCGCCGCTCGCGCC
GGTGAACAAGGACGTCGTCGCGAAGTTCGGCGACGCGTGGACGCGTCCCGGCAACATCGTCAGCAACGGCGCGTACCAGC
TCGTCGACTGGCAGCCGAACAACCGCATCGTGATGACGAAGGATGCGAAGTACTGGAACGCGCCGAAGGTCGTGATCAAC
AAGGTCACGTATCTGCCCGTCGAAAGCGACGAGACCGCGATGCGCATGTATCAGGCCGGGCAGATCGACTACAGCTACTC
GATTCCGTCGGGCATTTTCCAGCAGGTCAGCAAGCAGTTCGGCGCCGAGCTGCGCCCGGGCCTGCAGCTCGCGACGTACT
ACTACTACCTGAACAACAGCGATCCGGCGCTGAAGGACAAGCGCGTGCGCCAGGCGCTGTCGATGGTCATCGATCGCGAC
GTGCTGACGTCGAAGCTCACGCAGGCCGGCGAGAAGCCGATGTACGGCCTGATGCCGAACGGCACGAAGGGCGTGCAGCC
GTTCACGCCGGAATGGGCGTCCTGGCCGATGGCCAAGCGCGTCGAAACCGCGAAGAACCTGCTGAAGCAGGCCGGCTATT
CGGACGCGAAGCCGCTGTCGTTCACGCTCACGTACAACACCAACGACCTGCACAAGAAGGTCGCGCTGTTCGCCGCATCG
GAATGGCGCACGAAGCTCGGCATCGACACGAAGCTCGAGAACGTCGAGTTCAAGGTGCTGATGAAGCAGCGGCATGACGG
CAAGGTGCAGATCGCGCGCGACGGCTGGTTCGCCGACTACAACGACGCGATGACGTTCTTCGACCTGATCCGCTGCGGCA
GCTCGCAGAACACCGTCGGCTACTGCAACAAGCAGGTCGACACGCTCGTCGACGAGGGCAACCAGAAGCTCGACGACAAG
GCCCGCGCCGCACTGCTCACGCAGGCGCACGACACCGCGATGAAGGACACGCCGATGGTGCCGCTGTTCCAGTATTCGGC
CGATCGTCTCGTGAAGCCGTACGTCGGCGGCTATTCGCTGAAGAACGTGATCGACATGCGCGCGTCGCAGGACATGTATC
TGATCAAGCACTGA

Upstream 100 bases:

>100_bases
CCACGGGCCTCCTCAATCGCTGCCCCCGACAGCGTCTTACGCGTAATCCGCGGCCTGCGCCGCGCGACGCTCACCGAACA
ACCTTCTCAAGACTGCCACG

Downstream 100 bases:

>100_bases
GCGGAGCGATCATGCTGGCCTATGCCCTGAGGCGCACGTTGTGGGCGGTGCCGACGATCCTCGCGGTCGTCACCGTCTGC
TACCTGCTGCTGCACTTCAC

Product: peptide/nickel transport system substrate-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKSLHAMSAVLAALVLTTPAARAVTVPSNVTLAAQQDLTRQVPAEVESLDPAHIESWTGNTIGLDLFEGLARIDAAGQVV
PGVALSWERKTPQTWIFKLRHDAKWSNGQPVTAADFVYSWQRLADPKTGSKYTILVEFVKNSKDIIAGKAAPSTLGVRAV
DPYTLEVTTDVPVAFFPELTAMAPLAPVNKDVVAKFGDAWTRPGNIVSNGAYQLVDWQPNNRIVMTKDAKYWNAPKVVIN
KVTYLPVESDETAMRMYQAGQIDYSYSIPSGIFQQVSKQFGAELRPGLQLATYYYYLNNSDPALKDKRVRQALSMVIDRD
VLTSKLTQAGEKPMYGLMPNGTKGVQPFTPEWASWPMAKRVETAKNLLKQAGYSDAKPLSFTLTYNTNDLHKKVALFAAS
EWRTKLGIDTKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNKQVDTLVDEGNQKLDDK
ARAALLTQAHDTAMKDTPMVPLFQYSADRLVKPYVGGYSLKNVIDMRASQDMYLIKH

Sequences:

>Translated_537_residues
MKSLHAMSAVLAALVLTTPAARAVTVPSNVTLAAQQDLTRQVPAEVESLDPAHIESWTGNTIGLDLFEGLARIDAAGQVV
PGVALSWERKTPQTWIFKLRHDAKWSNGQPVTAADFVYSWQRLADPKTGSKYTILVEFVKNSKDIIAGKAAPSTLGVRAV
DPYTLEVTTDVPVAFFPELTAMAPLAPVNKDVVAKFGDAWTRPGNIVSNGAYQLVDWQPNNRIVMTKDAKYWNAPKVVIN
KVTYLPVESDETAMRMYQAGQIDYSYSIPSGIFQQVSKQFGAELRPGLQLATYYYYLNNSDPALKDKRVRQALSMVIDRD
VLTSKLTQAGEKPMYGLMPNGTKGVQPFTPEWASWPMAKRVETAKNLLKQAGYSDAKPLSFTLTYNTNDLHKKVALFAAS
EWRTKLGIDTKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNKQVDTLVDEGNQKLDDK
ARAALLTQAHDTAMKDTPMVPLFQYSADRLVKPYVGGYSLKNVIDMRASQDMYLIKH
>Mature_537_residues
MKSLHAMSAVLAALVLTTPAARAVTVPSNVTLAAQQDLTRQVPAEVESLDPAHIESWTGNTIGLDLFEGLARIDAAGQVV
PGVALSWERKTPQTWIFKLRHDAKWSNGQPVTAADFVYSWQRLADPKTGSKYTILVEFVKNSKDIIAGKAAPSTLGVRAV
DPYTLEVTTDVPVAFFPELTAMAPLAPVNKDVVAKFGDAWTRPGNIVSNGAYQLVDWQPNNRIVMTKDAKYWNAPKVVIN
KVTYLPVESDETAMRMYQAGQIDYSYSIPSGIFQQVSKQFGAELRPGLQLATYYYYLNNSDPALKDKRVRQALSMVIDRD
VLTSKLTQAGEKPMYGLMPNGTKGVQPFTPEWASWPMAKRVETAKNLLKQAGYSDAKPLSFTLTYNTNDLHKKVALFAAS
EWRTKLGIDTKLENVEFKVLMKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNKQVDTLVDEGNQKLDDK
ARAALLTQAHDTAMKDTPMVPLFQYSADRLVKPYVGGYSLKNVIDMRASQDMYLIKH

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=44.6529080675422, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI87081878, Length=539, Percent_Identity=39.5176252319109, Blast_Score=394, Evalue=1e-110,
Organism=Escherichia coli, GI1789397, Length=530, Percent_Identity=38.1132075471698, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1787762, Length=548, Percent_Identity=24.8175182481752, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787052, Length=483, Percent_Identity=23.1884057971014, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789966, Length=467, Percent_Identity=24.4111349036403, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789887, Length=518, Percent_Identity=24.1312741312741, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59727; Mature: 59727

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLHAMSAVLAALVLTTPAARAVTVPSNVTLAAQQDLTRQVPAEVESLDPAHIESWTGN
CCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHCCHHHHCCCCHHHHCCCCC
TIGLDLFEGLARIDAAGQVVPGVALSWERKTPQTWIFKLRHDAKWSNGQPVTAADFVYSW
EECHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
QRLADPKTGSKYTILVEFVKNSKDIIAGKAAPSTLGVRAVDPYTLEVTTDVPVAFFPELT
HHHCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCEEEECCEEEEEECCCCHHHCCCHH
AMAPLAPVNKDVVAKFGDAWTRPGNIVSNGAYQLVDWQPNNRIVMTKDAKYWNAPKVVIN
HHCCCCCCCHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCHHEEE
KVTYLPVESDETAMRMYQAGQIDYSYSIPSGIFQQVSKQFGAELRPGLQLATYYYYLNNS
EEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
DPALKDKRVRQALSMVIDRDVLTSKLTQAGEKPMYGLMPNGTKGVQPFTPEWASWPMAKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHH
VETAKNLLKQAGYSDAKPLSFTLTYNTNDLHKKVALFAASEWRTKLGIDTKLENVEFKVL
HHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEE
MKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNKQVDTLVDEGNQKLDDK
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHCCCCCHHHH
ARAALLTQAHDTAMKDTPMVPLFQYSADRLVKPYVGGYSLKNVIDMRASQDMYLIKH
HHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MKSLHAMSAVLAALVLTTPAARAVTVPSNVTLAAQQDLTRQVPAEVESLDPAHIESWTGN
CCHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEHHHHHHHCCHHHHCCCCHHHHCCCCC
TIGLDLFEGLARIDAAGQVVPGVALSWERKTPQTWIFKLRHDAKWSNGQPVTAADFVYSW
EECHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
QRLADPKTGSKYTILVEFVKNSKDIIAGKAAPSTLGVRAVDPYTLEVTTDVPVAFFPELT
HHHCCCCCCCEEEEEEEEECCCCCEEECCCCCCCCCEEEECCEEEEEECCCCHHHCCCHH
AMAPLAPVNKDVVAKFGDAWTRPGNIVSNGAYQLVDWQPNNRIVMTKDAKYWNAPKVVIN
HHCCCCCCCHHHHHHHCCCCCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCHHEEE
KVTYLPVESDETAMRMYQAGQIDYSYSIPSGIFQQVSKQFGAELRPGLQLATYYYYLNNS
EEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEECC
DPALKDKRVRQALSMVIDRDVLTSKLTQAGEKPMYGLMPNGTKGVQPFTPEWASWPMAKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHH
VETAKNLLKQAGYSDAKPLSFTLTYNTNDLHKKVALFAASEWRTKLGIDTKLENVEFKVL
HHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEE
MKQRHDGKVQIARDGWFADYNDAMTFFDLIRCGSSQNTVGYCNKQVDTLVDEGNQKLDDK
EECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHCCCCCHHHH
ARAALLTQAHDTAMKDTPMVPLFQYSADRLVKPYVGGYSLKNVIDMRASQDMYLIKH
HHHHHHHHHHHHHCCCCCCCEEECCCCHHHCCCCCCCCHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]