Click here to switch to the map view.

The map label for this gene is iciA [H]

Identifier: 189353960

GI number: 189353960

Start: 2276430

End: 2277335

Strand: Direct

Name: iciA [H]

Synonym: BMULJ_05213

Alternate gene names: 189353960

Gene position: 2276430-2277335 (Clockwise)

Preceding gene: 189353957

Following gene: 189353961

Centisome position: 92.05

GC content: 72.19

Gene sequence:

>906_bases
ATGTCGCTGACCATCGATCCGAAGCAGGCCGCCGCGCTGCTCGCCGTCGCCGATACGGGCAGCTTCGAGCAAGCGGCCGT
GCGGCTGCACGTGACCGCGTCGGCGATCACGCAGCGCGTGCGTGCGCTCGAAGCGAGCCTCGGCACGCCGCTCGTGCTGC
GCACGCGGCCGTGCCGTCCGACCGTCGCGGGGCAGCGCGTGCTGCAGCATCTGCGCCGCGTCGCGCTGCTGCAGGCGGAC
CTGCAAAGCACGCTCGCGACCGAGCGCGAAACGCCGATCTCGGTCACGATCGCGCTGAACTCGGACAGCCTCGGCACGTG
GTTTCTGCCGGCGCTGACATCGGTGCTCGCGGGCGAGCGCATCCTGTTCGAGCTGATCGTCGAGGATCAGGATCATACGT
TCGCGCTGCTCGAAAGCGGGATGGCGGTCGGCTGCGTGACGACCGAGCCGAAGCCGATGCGCGGCTGCGTCGCGACGCCG
CTCGGCACGATGCGCTATCGGCTGCTCGCGGCCGACGCGTTCGCCGCGCGCTGGTTTCCGCGCGGCCTCACACGCACGAG
CGCGCGCGATGCGCCGGTCGTCGCGTATTCGCGGCGCGACACGCTGCAGTCGTCGTTTCTGGAACAGCGGTTCGGGCTGC
CGCCCGGCGCGTACCCGTGCCACTACGTGCCCGGCACGCACGCGCATTTCGCGGCGGTGCGGCACGGCCTCGGCTACGCG
ATGGTGCCGGAGCCGCTGATCGGCGACGCGCCGCTCGCCGCGCAGCAGCTCGTCGATCTCGCGCCCGCGCATCCGACCGA
CGTCACGCTGTACTGGCACGCGTGGACCGTGCAGTCGCCGACGATGGCGTCGCTGTCCGCGCGCGTCGTCGAAGCGGCGC
GCCGCCTGCTCGCGCCGCTGCCGTAA

Upstream 100 bases:

>100_bases
CGCCGCGGTGGCGGTGCACGTCGCGCACGGCGCGACCGTCGATCGGGGGCTCGCTTACACTACGGGCCGTTCCGTTCGCG
TTCAGAGGAATTTCGTCGAC

Downstream 100 bases:

>100_bases
TCCGCGACGATCGCGCGACACGGCCTTCATCGCGGCAAAGGACACGTCGGGAAACCGGGCCGCGCCGCCTATACTTTTTG
CATCGGCCGCGCGAACGCGC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSLTIDPKQAAALLAVADTGSFEQAAVRLHVTASAITQRVRALEASLGTPLVLRTRPCRPTVAGQRVLQHLRRVALLQAD
LQSTLATERETPISVTIALNSDSLGTWFLPALTSVLAGERILFELIVEDQDHTFALLESGMAVGCVTTEPKPMRGCVATP
LGTMRYRLLAADAFAARWFPRGLTRTSARDAPVVAYSRRDTLQSSFLEQRFGLPPGAYPCHYVPGTHAHFAAVRHGLGYA
MVPEPLIGDAPLAAQQLVDLAPAHPTDVTLYWHAWTVQSPTMASLSARVVEAARRLLAPLP

Sequences:

>Translated_301_residues
MSLTIDPKQAAALLAVADTGSFEQAAVRLHVTASAITQRVRALEASLGTPLVLRTRPCRPTVAGQRVLQHLRRVALLQAD
LQSTLATERETPISVTIALNSDSLGTWFLPALTSVLAGERILFELIVEDQDHTFALLESGMAVGCVTTEPKPMRGCVATP
LGTMRYRLLAADAFAARWFPRGLTRTSARDAPVVAYSRRDTLQSSFLEQRFGLPPGAYPCHYVPGTHAHFAAVRHGLGYA
MVPEPLIGDAPLAAQQLVDLAPAHPTDVTLYWHAWTVQSPTMASLSARVVEAARRLLAPLP
>Mature_300_residues
SLTIDPKQAAALLAVADTGSFEQAAVRLHVTASAITQRVRALEASLGTPLVLRTRPCRPTVAGQRVLQHLRRVALLQADL
QSTLATERETPISVTIALNSDSLGTWFLPALTSVLAGERILFELIVEDQDHTFALLESGMAVGCVTTEPKPMRGCVATPL
GTMRYRLLAADAFAARWFPRGLTRTSARDAPVVAYSRRDTLQSSFLEQRFGLPPGAYPCHYVPGTHAHFAAVRHGLGYAM
VPEPLIGDAPLAAQQLVDLAPAHPTDVTLYWHAWTVQSPTMASLSARVVEAARRLLAPLP

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=294, Percent_Identity=37.7551020408163, Blast_Score=197, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32524; Mature: 32393

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTIDPKQAAALLAVADTGSFEQAAVRLHVTASAITQRVRALEASLGTPLVLRTRPCRP
CCEECCHHHHEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCC
TVAGQRVLQHLRRVALLQADLQSTLATERETPISVTIALNSDSLGTWFLPALTSVLAGER
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHH
ILFELIVEDQDHTFALLESGMAVGCVTTEPKPMRGCVATPLGTMRYRLLAADAFAARWFP
HEEEEEECCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RGLTRTSARDAPVVAYSRRDTLQSSFLEQRFGLPPGAYPCHYVPGTHAHFAAVRHGLGYA
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
MVPEPLIGDAPLAAQQLVDLAPAHPTDVTLYWHAWTVQSPTMASLSARVVEAARRLLAPL
ECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
P
C
>Mature Secondary Structure 
SLTIDPKQAAALLAVADTGSFEQAAVRLHVTASAITQRVRALEASLGTPLVLRTRPCRP
CEECCHHHHEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEECCCCCC
TVAGQRVLQHLRRVALLQADLQSTLATERETPISVTIALNSDSLGTWFLPALTSVLAGER
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCHH
ILFELIVEDQDHTFALLESGMAVGCVTTEPKPMRGCVATPLGTMRYRLLAADAFAARWFP
HEEEEEECCCCCCHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
RGLTRTSARDAPVVAYSRRDTLQSSFLEQRFGLPPGAYPCHYVPGTHAHFAAVRHGLGYA
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCE
MVPEPLIGDAPLAAQQLVDLAPAHPTDVTLYWHAWTVQSPTMASLSARVVEAARRLLAPL
ECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9286976 [H]