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The map label for this gene is lipA [H]

Identifier: 189353902

GI number: 189353902

Start: 2212907

End: 2213767

Strand: Reverse

Name: lipA [H]

Synonym: BMULJ_05154

Alternate gene names: 189353902

Gene position: 2213767-2212907 (Counterclockwise)

Preceding gene: 189353905

Following gene: 189353901

Centisome position: 89.51

GC content: 67.25

Gene sequence:

>861_bases
ATGGACGCTTCCGAATTCAGCAAATTCCTGAAATCCGCACTGCCCGCCGAAGCCAGAGCCGGCGCGGCACTGGCGGTCAC
GGAGCTGGAAATTCCGGGCTACGCGCAGGACATCGCGCTGCGCCTCTATCGCCGCCCCGACAAGACCGGGTTGCCAGTAG
TGCTTTATTTCCACGGCGGCGGTTTCGTGCGCGGCACGCTCGACGACGCGGATTTCGCCGCGCGCTTTTTAGCAGAACGC
TTACCGGCGCTCGTAGTGTCGGTCGACTATTCGCTCGCGCCCGCGTTTCCGTTTCCGGCCGCGCCGGAAGACGCGTATCG
CGCGGCCGTCTGGGCCGCGACGCGCGCCCGTGCGTTCGGCGGCAATCCGAAGAAGATCGGCGTCGCGGGACACGACGCGG
GCGGACAGCTCGCGAACTGTCTCGCGTTCATCGCGCGCGATCGCGGCGACGTGCCGATCGTCGCGCAGGCGCTGTTCGGG
CCGATGCTCGACCCGAGCATGACGCGCATCGGCGATGCCGAACGTCTCGCGTCGGACATCACCGCACGCGAATGCGCGGC
CTGTTATCGCGCCTATCTGCCGCAGGCGTCGCAACGCATGCATCCGTACGCGGCGCCGCTCGAGTCGGTGCGCCTCTCGG
GGCTGCCGCCGACGCTGATCGTCACCGCGCAGAACGACGTGCTGCACGTCGAAGCGGAGAAATACGCGGGCTGCCTGATT
TCGTCGGGCGTGCTGACGCAGGTGATCCGCTATCCGGACGTCACGCATGCCGCGCTCGCGACGCACGAAGCCGCCTTCGA
CGAAGCCGTGCGCTTCTTCCAGTGCCGCTTCCAGGCGCGCCAGCCGAATCGCAACGAATAA

Upstream 100 bases:

>100_bases
TGTTCGGGGGCGTTGAATTGTGTTGCCGGATTATAGGCATTTATCCGGATGTCTGCCGGACCCAGAATTCGATCCATCTC
GAAATGGACGATGCATCGTC

Downstream 100 bases:

>100_bases
CCAATCCAATCCACGCTTACCGGAGATCTACATGGCCATCCTACGCACCTCCCGCTCCCGCATCGCGACCGCTGCGGTCG
CGACGCTCGCCGTCGTCGGC

Product: triacylglycerol lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MDASEFSKFLKSALPAEARAGAALAVTELEIPGYAQDIALRLYRRPDKTGLPVVLYFHGGGFVRGTLDDADFAARFLAER
LPALVVSVDYSLAPAFPFPAAPEDAYRAAVWAATRARAFGGNPKKIGVAGHDAGGQLANCLAFIARDRGDVPIVAQALFG
PMLDPSMTRIGDAERLASDITARECAACYRAYLPQASQRMHPYAAPLESVRLSGLPPTLIVTAQNDVLHVEAEKYAGCLI
SSGVLTQVIRYPDVTHAALATHEAAFDEAVRFFQCRFQARQPNRNE

Sequences:

>Translated_286_residues
MDASEFSKFLKSALPAEARAGAALAVTELEIPGYAQDIALRLYRRPDKTGLPVVLYFHGGGFVRGTLDDADFAARFLAER
LPALVVSVDYSLAPAFPFPAAPEDAYRAAVWAATRARAFGGNPKKIGVAGHDAGGQLANCLAFIARDRGDVPIVAQALFG
PMLDPSMTRIGDAERLASDITARECAACYRAYLPQASQRMHPYAAPLESVRLSGLPPTLIVTAQNDVLHVEAEKYAGCLI
SSGVLTQVIRYPDVTHAALATHEAAFDEAVRFFQCRFQARQPNRNE
>Mature_286_residues
MDASEFSKFLKSALPAEARAGAALAVTELEIPGYAQDIALRLYRRPDKTGLPVVLYFHGGGFVRGTLDDADFAARFLAER
LPALVVSVDYSLAPAFPFPAAPEDAYRAAVWAATRARAFGGNPKKIGVAGHDAGGQLANCLAFIARDRGDVPIVAQALFG
PMLDPSMTRIGDAERLASDITARECAACYRAYLPQASQRMHPYAAPLESVRLSGLPPTLIVTAQNDVLHVEAEKYAGCLI
SSGVLTQVIRYPDVTHAALATHEAAFDEAVRFFQCRFQARQPNRNE

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=327, Percent_Identity=27.5229357798165, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI206597554, Length=294, Percent_Identity=25.8503401360544, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI21328446, Length=109, Percent_Identity=36.697247706422, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=235, Percent_Identity=28.936170212766, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 30834; Mature: 30834

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASEFSKFLKSALPAEARAGAALAVTELEIPGYAQDIALRLYRRPDKTGLPVVLYFHGG
CCHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECC
GFVRGTLDDADFAARFLAERLPALVVSVDYSLAPAFPFPAAPEDAYRAAVWAATRARAFG
CEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GNPKKIGVAGHDAGGQLANCLAFIARDRGDVPIVAQALFGPMLDPSMTRIGDAERLASDI
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHH
TARECAACYRAYLPQASQRMHPYAAPLESVRLSGLPPTLIVTAQNDVLHVEAEKYAGCLI
HHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEEHHHHHHHHH
SSGVLTQVIRYPDVTHAALATHEAAFDEAVRFFQCRFQARQPNRNE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDASEFSKFLKSALPAEARAGAALAVTELEIPGYAQDIALRLYRRPDKTGLPVVLYFHGG
CCHHHHHHHHHHHCCCHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEECC
GFVRGTLDDADFAARFLAERLPALVVSVDYSLAPAFPFPAAPEDAYRAAVWAATRARAFG
CEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GNPKKIGVAGHDAGGQLANCLAFIARDRGDVPIVAQALFGPMLDPSMTRIGDAERLASDI
CCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHHCCCHHHHHHHH
TARECAACYRAYLPQASQRMHPYAAPLESVRLSGLPPTLIVTAQNDVLHVEAEKYAGCLI
HHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEEHHHHHHHHH
SSGVLTQVIRYPDVTHAALATHEAAFDEAVRFFQCRFQARQPNRNE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA