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The map label for this gene is nadE [H]

Identifier: 189353826

GI number: 189353826

Start: 2127772

End: 2128650

Strand: Direct

Name: nadE [H]

Synonym: BMULJ_05073

Alternate gene names: 189353826

Gene position: 2127772-2128650 (Clockwise)

Preceding gene: 189353825

Following gene: 189353829

Centisome position: 86.03

GC content: 68.71

Gene sequence:

>879_bases
ATGACAGCCGCCGATTACGCCAGCCGCCAACGCGCGATCATCGCCGAACTGAACGTTGCCCCGCACTTCGATGCCGACGC
CGAAATCGCGCGCCGCGTCGATTTCCTCGCGCAATATCTGCGCTCGACCGGCCTGCGGACCTATGTGCTCGGCATCAGCG
GCGGCGTCGATTCGTCGACGGCCGGCCGCCTCGCGCAACTCGCGGTCGAACGCCTGCGCGGCGAGGGCTACGATGCGCGC
TTCATCGCGATGCGTCTGCCGAACGGCGTGCAGAACGACGAGGCCGACGCACAGCGCGCGCTCGCGTTCGTGCGCGCGGA
CGAAGTGTTGACGGTCGACGTGAAGCCCGCCGCCGACGCGATGCTGAAGTCGCTGATCGCCTCCGGCCACGCCTTCGACA
CGCCGGCACAGCAGGATTTCGTGCACGGCAACATCAAGGCGCGCGAGCGGATGATCGCGCAATACGCGGTGGCCGGCGCG
CGCCGCGGCATCGTGATCGGCACCGATCACGCGGCCGAATCGCTGATGGGCTTCTTCACGAAGTTCGGCGACGGCGGCGC
GGACATCCTGCCGCTCGCCGGCCTGAACAAGCGTCGCGTGCGCGCGGTCGCGCGTGCGCTCGGCGCCGAAGACGCGGTCG
TGATGAAGGTGCCGACCGCCGATCTGGAAGAGCTGCGTCCGCTGCGTCCGGACGAGCACGCGTACGGCGTCACGTACGAC
GAGATCGACGATTTCCTCGAAGGCAAGCCGGTCGAAGACCGCGTGTACGAAACGGTCCTGCGCTTCTACGACGGCTCGCG
CCACAAGCGCGCGCTGCCGTACACACCGTTCGACTGGCCGGGCCTCGGTATCGCCTCGAACGCCGGCCACGGCGCCTGA

Upstream 100 bases:

>100_bases
CATGCGCAACGCCCGTCCGCACAATCGCCCCCGGCGCGCGGCAGCGCACGGCGATTGCGCTTATGATGAGCGCCTTGCAT
TTCGTTCCGGAACTTTCCCC

Downstream 100 bases:

>100_bases
CGCGGCCGGGCCCACCGATACCGGCGAGCGGCCGCGCGCGACACCGTTGCGCACGCGGCCGGGCAAGCGGGCACGACGCT
ACGCGTGCCCGCCCGTCTTC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTAADYASRQRAIIAELNVAPHFDADAEIARRVDFLAQYLRSTGLRTYVLGISGGVDSSTAGRLAQLAVERLRGEGYDAR
FIAMRLPNGVQNDEADAQRALAFVRADEVLTVDVKPAADAMLKSLIASGHAFDTPAQQDFVHGNIKARERMIAQYAVAGA
RRGIVIGTDHAAESLMGFFTKFGDGGADILPLAGLNKRRVRAVARALGAEDAVVMKVPTADLEELRPLRPDEHAYGVTYD
EIDDFLEGKPVEDRVYETVLRFYDGSRHKRALPYTPFDWPGLGIASNAGHGA

Sequences:

>Translated_292_residues
MTAADYASRQRAIIAELNVAPHFDADAEIARRVDFLAQYLRSTGLRTYVLGISGGVDSSTAGRLAQLAVERLRGEGYDAR
FIAMRLPNGVQNDEADAQRALAFVRADEVLTVDVKPAADAMLKSLIASGHAFDTPAQQDFVHGNIKARERMIAQYAVAGA
RRGIVIGTDHAAESLMGFFTKFGDGGADILPLAGLNKRRVRAVARALGAEDAVVMKVPTADLEELRPLRPDEHAYGVTYD
EIDDFLEGKPVEDRVYETVLRFYDGSRHKRALPYTPFDWPGLGIASNAGHGA
>Mature_291_residues
TAADYASRQRAIIAELNVAPHFDADAEIARRVDFLAQYLRSTGLRTYVLGISGGVDSSTAGRLAQLAVERLRGEGYDARF
IAMRLPNGVQNDEADAQRALAFVRADEVLTVDVKPAADAMLKSLIASGHAFDTPAQQDFVHGNIKARERMIAQYAVAGAR
RGIVIGTDHAAESLMGFFTKFGDGGADILPLAGLNKRRVRAVARALGAEDAVVMKVPTADLEELRPLRPDEHAYGVTYDE
IDDFLEGKPVEDRVYETVLRFYDGSRHKRALPYTPFDWPGLGIASNAGHGA

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=272, Percent_Identity=50.7352941176471, Blast_Score=253, Evalue=1e-68,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 31728; Mature: 31597

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAADYASRQRAIIAELNVAPHFDADAEIARRVDFLAQYLRSTGLRTYVLGISGGVDSST
CCCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
AGRLAQLAVERLRGEGYDARFIAMRLPNGVQNDEADAQRALAFVRADEVLTVDVKPAADA
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHH
MLKSLIASGHAFDTPAQQDFVHGNIKARERMIAQYAVAGARRGIVIGTDHAAESLMGFFT
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
KFGDGGADILPLAGLNKRRVRAVARALGAEDAVVMKVPTADLEELRPLRPDEHAYGVTYD
HHCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHH
EIDDFLEGKPVEDRVYETVLRFYDGSRHKRALPYTPFDWPGLGIASNAGHGA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAADYASRQRAIIAELNVAPHFDADAEIARRVDFLAQYLRSTGLRTYVLGISGGVDSST
CCCHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHH
AGRLAQLAVERLRGEGYDARFIAMRLPNGVQNDEADAQRALAFVRADEVLTVDVKPAADA
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHH
MLKSLIASGHAFDTPAQQDFVHGNIKARERMIAQYAVAGARRGIVIGTDHAAESLMGFFT
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHH
KFGDGGADILPLAGLNKRRVRAVARALGAEDAVVMKVPTADLEELRPLRPDEHAYGVTYD
HHCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCHH
EIDDFLEGKPVEDRVYETVLRFYDGSRHKRALPYTPFDWPGLGIASNAGHGA
HHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA