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The map label for this gene is proW [H]

Identifier: 189353784

GI number: 189353784

Start: 2063891

End: 2064787

Strand: Reverse

Name: proW [H]

Synonym: BMULJ_05031

Alternate gene names: 189353784

Gene position: 2064787-2063891 (Counterclockwise)

Preceding gene: 189353785

Following gene: 189353783

Centisome position: 83.49

GC content: 65.33

Gene sequence:

>897_bases
ATGTCTGAAATGATTCCGCTCGGTAGTTGGGTCGACCAGTCCGTTCATTACCTGCTCGACCATGATGCCGCGACGTTCGA
TGCGGTCGGTCGCGCGATCGAAGGGCTGGCCGCACTGATCGAGCACGGCCTGCAGGCCGTGCCGATGTGGCTGATGATGG
CGTTCTTCATCGGCATCGGGCTGTGGCGCGTGGGCTGGCGCTTCGCGCTGTTCACCACCTTGTCGCTGCTGCTGATCTTC
GCGACCGGCTTCTGGGATCAGACGGTGATCACGCTGGGCCTCACGCTGTCGTCGACGATCATCAGCCTCGCGCTCGGCAT
TCCGCTCGGCATCTGGGCCGCGAAGAGCAAGTGGGTCGCCGCCATCGTGCGTCCGATCCTCGACCTGATGCAGACGATGC
CCGCCTTCGTCTACCTGATTCCGGCCGCGATGCTGTTCGGTCTCGGCCGCGTGCCGGGGATCCTGTCGACGGTGATCTTC
GCGATGCCGCCGGCCGTGCGTCTGACGAGCCTCGGCATCCGCCACGTGAACCGCGAGATCGTCGAAGCCGGCCAGGCATT
CGGCTGCACGCCGTGGCAGCTGCTCTACAAGGTGCAGTTCCCGAACGCGCTGCCGTCGATCATGCAGGGCGTGAACCAGA
CGATCATGATGGCGCTGTCGATGGTGATCATCGCGTCGATGGTCGGCGCGGGCGGCCTCGGCAACGACGTGCTCGCGAGC
ATCCAGCGTCTCGACATCGGTCTCGGCTTCGAAAGCGGTCTGTCGGTCGTGCTGCTCGCGATCATTCTCGACCGCATCAC
CGAAAGCTTCGGCCGTGCGCCGGGCGCCGCGCGAGCACCGCTGTTCGCCGGTCTCAAGCAACTGTTTCGCGTGAAGGCCG
CGCCCGCGCAAGCCTGA

Upstream 100 bases:

>100_bases
AGCCCGAACGCATTGCCCGTCGTCGACGACGACGGCTGCTACTGCGGTTCGGTCGACCGCGCGCTCATCCTGAAGGCCAT
CACGCGTTCGCGAGGCTCCC

Downstream 100 bases:

>100_bases
TCGCCCGGCGGCGGGCGCCTCGTCCCGCCGCCTTTCCCTCGTCCAGTTCCTGCTCCCGCGGTTCGCGCCGCGGGGCAGGT
TTCGTGCAACCTCTCGATAG

Product: glycine betaine/proline transport system permease protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSEMIPLGSWVDQSVHYLLDHDAATFDAVGRAIEGLAALIEHGLQAVPMWLMMAFFIGIGLWRVGWRFALFTTLSLLLIF
ATGFWDQTVITLGLTLSSTIISLALGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGLKQLFRVKAAPAQA

Sequences:

>Translated_298_residues
MSEMIPLGSWVDQSVHYLLDHDAATFDAVGRAIEGLAALIEHGLQAVPMWLMMAFFIGIGLWRVGWRFALFTTLSLLLIF
ATGFWDQTVITLGLTLSSTIISLALGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIF
AMPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGLKQLFRVKAAPAQA
>Mature_297_residues
SEMIPLGSWVDQSVHYLLDHDAATFDAVGRAIEGLAALIEHGLQAVPMWLMMAFFIGIGLWRVGWRFALFTTLSLLLIFA
TGFWDQTVITLGLTLSSTIISLALGIPLGIWAAKSKWVAAIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFA
MPPAVRLTSLGIRHVNREIVEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLASI
QRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGLKQLFRVKAAPAQA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=271, Percent_Identity=46.1254612546125, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788451, Length=201, Percent_Identity=28.3582089552239, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788449, Length=180, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32015; Mature: 31884

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMIPLGSWVDQSVHYLLDHDAATFDAVGRAIEGLAALIEHGLQAVPMWLMMAFFIGIG
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFALFTTLSLLLIFATGFWDQTVITLGLTLSSTIISLALGIPLGIWAAKSKWVA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRHVNREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGLKQLFRVKAAPAQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEMIPLGSWVDQSVHYLLDHDAATFDAVGRAIEGLAALIEHGLQAVPMWLMMAFFIGIG
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWRVGWRFALFTTLSLLLIFATGFWDQTVITLGLTLSSTIISLALGIPLGIWAAKSKWVA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
AIVRPILDLMQTMPAFVYLIPAAMLFGLGRVPGILSTVIFAMPPAVRLTSLGIRHVNREI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VEAGQAFGCTPWQLLYKVQFPNALPSIMQGVNQTIMMALSMVIIASMVGAGGLGNDVLAS
HHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
IQRLDIGLGFESGLSVVLLAIILDRITESFGRAPGAARAPLFAGLKQLFRVKAAPAQA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]