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The map label for this gene is fadH [H]

Identifier: 189353723

GI number: 189353723

Start: 1990134

End: 1992197

Strand: Reverse

Name: fadH [H]

Synonym: BMULJ_04968

Alternate gene names: 189353723

Gene position: 1992197-1990134 (Counterclockwise)

Preceding gene: 189353724

Following gene: 189353722

Centisome position: 80.55

GC content: 66.52

Gene sequence:

>2064_bases
ATGCGTTATCCCCACCTGTTCAAACCCATGCAGCTCAATCAGCTGACGCTGCGTAACCGGATCGTCAGTACCGCGCACGC
GGAGGTGTATGCCGAGCCGGGTGGCCTGCCGGGCGACCGCTACATCCGCTACTACGAGGAGAAGGCGAAGGGCGGTGTCG
GCCTCGCGATCTGCGGCGGCTCGAGCCCCGTGTCGATCGACAGCCCGCAGGGCTGGTGGAAGTCGGTGAACCTGTCGAAC
GACAAGATCATCGACCCGCTCACGCGGCTCGCCGACGCGATGCACAAGCACGGCGCGAAGATCATGATCCAGGCGACGCA
CATGGGCCGCCGCTCGTCGTTCCACGGCGAGCACTGGCCGCATCTGATGTCGCCGTCGGGCGTGCGCGAGCCGGTGCACC
GCGGCAACGCGAAGATCATCGAGATCGAGGAGATCCGCCGCATCATCGGCGATTTCGCCGCCGCCGCGAAGCGCGTGCAG
GCGGCCGGCATGGACGGCATCGAAATCTCGGCCGCACACCAGCATCTGATCGACCAGTTCTGGAGCCCGCGCTCGAACCA
CCGCACCGACGAATGGGGCGGCAGCCTGCAGAACCGTCTGCGCTTCGGCATCGAGGTGTTGACGGCCGTACGCGAAGCCG
TCGGCAAGGATTTCTGCGTCGGCCTGCGGATGTGCGGCGACGAATTTCACGAGGACGGCCTGGACCACGAAGCGCTGAAG
GAAATCGCGCAGGCGATGGCCGAGACGGGGCTGATCGACTACCTGAGCGTCGTCGGCTCGGGCGGCGATACACACAACAC
GATCGCGAACTGCATGCCGCCGATGGCGCTGCCGCCGGAGCCGTTCGTGCACCTCGCGGCCGGCATCAAGTCGGTCGTCA
AGATTCCGGTGATGCACGCGCAGAGCATCCGCGACGCCGGGCAGGCCGAGCGCCTGCTCGCGAACGGGATGATCGACCTG
GTCGGCATGACGCGCGCGCAGATCGCCGATCCGCACATGGTGATCAAGATCCGCGACGGCCGCGAGGACGAAATCAAGCA
GTGCGTCGGCGCGAACTACTGCATCGACCGCCAGTACAACGGCCTCGACGTGCTCTGCATCCAGAACGCGGCGACCTCGC
GCGAAGCGACGATGCCGCACATCATCGAGAAGTCGCGCGGCCCGAAGCGCAAGGTCGTCGTCGTTGGCGCGGGCCCGGCC
GGGCTCGAGGCGGCGCGCGTGGCACGCCTGCGCGGCCACGACGTCGTGCTGTTCGAGAAGAACGCGGAAGTCGGCGGCCA
GGTGATGATCGCCGCGAAGGCGCCGCAGCGCGAACAGATGTCGGGGATCATCCGCTGGTTCGACATGGAGACGAAGCGCA
TCGGCGTCGACCGGCGTCTCGGCGTGACGGCCGACGAGAAGATGATCATGGCCGAGAAGCCGGACATCGTCGTGCTCGCG
ACCGGCGGCTCGAGCTTCACGTGGCAGGTGCCGGCGTGGGGCGTCGCCGAAGGGCTCGCCGTCAGCTCGTGGGACATCCT
GACCGGCAAGGTCGAGCCGAAGCAGAACGTGCTGCTGTTCGACGGCGTGAGCACGCATGCGGGCGCGGGCGTCGCCGACT
TCATGGCGAGCCGCGGCTCGAAGGTCGAGGTCGTCACGCCCGACGTGAAGGTCGCCGACGACTGCGGCGGCACGACGTTC
CCGATCTTCTATCGCCGGCTGTACGCGCTCGGCGTGATCCAGACGCCGAACACGATGCTCGACCGCGTGTACGAGGAAGA
CGGCAAGAAGATCGCGGTGCTGCGCAACGAATACACCGAGGAACTGGAAGAGCGCGCGGTCGACCAGGTCGTGATCGAGA
ACGGTTCGTCGCCGAACGACGAGCTCTACTGGAAGCTGAAGCCGGAATCGGTGAACCGCGGCCAGATCGATCCGCACACG
CTGTTCGCGGCCGAGCCGCAGCCGTGCCTGTCGGAAGAACTCGGCAACGGCCGCTTCCTGCTGTTCCGTGTCGGCGACTG
CATCTCGATGCACAACGTCCACGGTGCGATCTACGACTCGCTGCGTCTCGTGAAGGATTTCTGA

Upstream 100 bases:

>100_bases
CGACCGCTGCATCTTCGAAGTGTCGCCGCTCGCGCATTGATGCACTGAAACAGAACAACACCCGCAACACGAGACATTCG
AACGCCAGAGGTCGCCAGCG

Downstream 100 bases:

>100_bases
CGATGAATCCGGCATTCCTGATTACCGCGTTGCTGTGGCTGTCGGTGGCGGGGCTCGCGTTCGCGGTCGCGAAGCGCTCG
TCGTACTGGCGTCTCGGGCG

Product: NADPH2-dependent 2,4-dienoyl-CoA reductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MRYPHLFKPMQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLSN
DKIIDPLTRLADAMHKHGAKIMIQATHMGRRSSFHGEHWPHLMSPSGVREPVHRGNAKIIEIEEIRRIIGDFAAAAKRVQ
AAGMDGIEISAAHQHLIDQFWSPRSNHRTDEWGGSLQNRLRFGIEVLTAVREAVGKDFCVGLRMCGDEFHEDGLDHEALK
EIAQAMAETGLIDYLSVVGSGGDTHNTIANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLANGMIDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCIQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARLRGHDVVLFEKNAEVGGQVMIAAKAPQREQMSGIIRWFDMETKRIGVDRRLGVTADEKMIMAEKPDIVVLA
TGGSSFTWQVPAWGVAEGLAVSSWDILTGKVEPKQNVLLFDGVSTHAGAGVADFMASRGSKVEVVTPDVKVADDCGGTTF
PIFYRRLYALGVIQTPNTMLDRVYEEDGKKIAVLRNEYTEELEERAVDQVVIENGSSPNDELYWKLKPESVNRGQIDPHT
LFAAEPQPCLSEELGNGRFLLFRVGDCISMHNVHGAIYDSLRLVKDF

Sequences:

>Translated_687_residues
MRYPHLFKPMQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLSN
DKIIDPLTRLADAMHKHGAKIMIQATHMGRRSSFHGEHWPHLMSPSGVREPVHRGNAKIIEIEEIRRIIGDFAAAAKRVQ
AAGMDGIEISAAHQHLIDQFWSPRSNHRTDEWGGSLQNRLRFGIEVLTAVREAVGKDFCVGLRMCGDEFHEDGLDHEALK
EIAQAMAETGLIDYLSVVGSGGDTHNTIANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLANGMIDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCIQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARLRGHDVVLFEKNAEVGGQVMIAAKAPQREQMSGIIRWFDMETKRIGVDRRLGVTADEKMIMAEKPDIVVLA
TGGSSFTWQVPAWGVAEGLAVSSWDILTGKVEPKQNVLLFDGVSTHAGAGVADFMASRGSKVEVVTPDVKVADDCGGTTF
PIFYRRLYALGVIQTPNTMLDRVYEEDGKKIAVLRNEYTEELEERAVDQVVIENGSSPNDELYWKLKPESVNRGQIDPHT
LFAAEPQPCLSEELGNGRFLLFRVGDCISMHNVHGAIYDSLRLVKDF
>Mature_687_residues
MRYPHLFKPMQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGGSSPVSIDSPQGWWKSVNLSN
DKIIDPLTRLADAMHKHGAKIMIQATHMGRRSSFHGEHWPHLMSPSGVREPVHRGNAKIIEIEEIRRIIGDFAAAAKRVQ
AAGMDGIEISAAHQHLIDQFWSPRSNHRTDEWGGSLQNRLRFGIEVLTAVREAVGKDFCVGLRMCGDEFHEDGLDHEALK
EIAQAMAETGLIDYLSVVGSGGDTHNTIANCMPPMALPPEPFVHLAAGIKSVVKIPVMHAQSIRDAGQAERLLANGMIDL
VGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYNGLDVLCIQNAATSREATMPHIIEKSRGPKRKVVVVGAGPA
GLEAARVARLRGHDVVLFEKNAEVGGQVMIAAKAPQREQMSGIIRWFDMETKRIGVDRRLGVTADEKMIMAEKPDIVVLA
TGGSSFTWQVPAWGVAEGLAVSSWDILTGKVEPKQNVLLFDGVSTHAGAGVADFMASRGSKVEVVTPDVKVADDCGGTTF
PIFYRRLYALGVIQTPNTMLDRVYEEDGKKIAVLRNEYTEELEERAVDQVVIENGSSPNDELYWKLKPESVNRGQIDPHT
LFAAEPQPCLSEELGNGRFLLFRVGDCISMHNVHGAIYDSLRLVKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=671, Percent_Identity=31.2965722801788, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787939, Length=360, Percent_Identity=26.1111111111111, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17566914, Length=336, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=345, Percent_Identity=27.536231884058, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=375, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=344, Percent_Identity=23.8372093023256, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=337, Percent_Identity=25.8160237388724, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=369, Percent_Identity=26.2872628726287, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=348, Percent_Identity=27.0114942528736, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=242, Percent_Identity=30.5785123966942, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325086, Length=246, Percent_Identity=28.8617886178862, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 75586; Mature: 75586

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYPHLFKPMQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGG
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSNDKIIDPLTRLADAMHKHGAKIMIQATHMGRRSSFHGEHWP
CCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMSPSGVREPVHRGNAKIIEIEEIRRIIGDFAAAAKRVQAAGMDGIEISAAHQHLIDQF
CCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRSNHRTDEWGGSLQNRLRFGIEVLTAVREAVGKDFCVGLRMCGDEFHEDGLDHEALK
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMAETGLIDYLSVVGSGGDTHNTIANCMPPMALPPEPFVHLAAGIKSVVKIPVMHA
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLANGMIDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCC
GLDVLCIQNAATSREATMPHIIEKSRGPKRKVVVVGAGPAGLEAARVARLRGHDVVLFEK
CEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
NAEVGGQVMIAAKAPQREQMSGIIRWFDMETKRIGVDRRLGVTADEKMIMAEKPDIVVLA
CCCCCCEEEEEECCCCHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCEEEECCCCEEEEE
TGGSSFTWQVPAWGVAEGLAVSSWDILTGKVEPKQNVLLFDGVSTHAGAGVADFMASRGS
ECCCCEEEECCCCCCCCCCEECCCEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHCCCC
KVEVVTPDVKVADDCGGTTFPIFYRRLYALGVIQTPNTMLDRVYEEDGKKIAVLRNEYTE
EEEEECCCCEEECCCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHCCCEEEEEHHHHHH
ELEERAVDQVVIENGSSPNDELYWKLKPESVNRGQIDPHTLFAAEPQPCLSEELGNGRFL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
LFRVGDCISMHNVHGAIYDSLRLVKDF
EEECCCCHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYPHLFKPMQLNQLTLRNRIVSTAHAEVYAEPGGLPGDRYIRYYEEKAKGGVGLAICGG
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
SSPVSIDSPQGWWKSVNLSNDKIIDPLTRLADAMHKHGAKIMIQATHMGRRSSFHGEHWP
CCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCC
HLMSPSGVREPVHRGNAKIIEIEEIRRIIGDFAAAAKRVQAAGMDGIEISAAHQHLIDQF
CCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
WSPRSNHRTDEWGGSLQNRLRFGIEVLTAVREAVGKDFCVGLRMCGDEFHEDGLDHEALK
CCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
EIAQAMAETGLIDYLSVVGSGGDTHNTIANCMPPMALPPEPFVHLAAGIKSVVKIPVMHA
HHHHHHHHCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QSIRDAGQAERLLANGMIDLVGMTRAQIADPHMVIKIRDGREDEIKQCVGANYCIDRQYN
HHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEECCCCC
GLDVLCIQNAATSREATMPHIIEKSRGPKRKVVVVGAGPAGLEAARVARLRGHDVVLFEK
CEEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEC
NAEVGGQVMIAAKAPQREQMSGIIRWFDMETKRIGVDRRLGVTADEKMIMAEKPDIVVLA
CCCCCCEEEEEECCCCHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCEEEECCCCEEEEE
TGGSSFTWQVPAWGVAEGLAVSSWDILTGKVEPKQNVLLFDGVSTHAGAGVADFMASRGS
ECCCCEEEECCCCCCCCCCEECCCEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHCCCC
KVEVVTPDVKVADDCGGTTFPIFYRRLYALGVIQTPNTMLDRVYEEDGKKIAVLRNEYTE
EEEEECCCCEEECCCCCCCHHHHHHHHHHHCEECCCHHHHHHHHHHCCCEEEEEHHHHHH
ELEERAVDQVVIENGSSPNDELYWKLKPESVNRGQIDPHTLFAAEPQPCLSEELGNGRFL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHCCCCCEE
LFRVGDCISMHNVHGAIYDSLRLVKDF
EEECCCCHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]