Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 189353579

GI number: 189353579

Start: 1829810

End: 1830670

Strand: Reverse

Name: prpB [C]

Synonym: BMULJ_04823

Alternate gene names: 189353579

Gene position: 1830670-1829810 (Counterclockwise)

Preceding gene: 189353580

Following gene: 189353578

Centisome position: 74.02

GC content: 70.96

Gene sequence:

>861_bases
ATGCCGTATGCCGCTCACCAGACCCGCCACGCCGAAACGTTCCGCGCGCTGCACGCGCGCGCCGGCGCATTCGTGATTCC
GAATCCGTGGGACGCCGGCACCGCGCGGCTGCTCGCGATGGCCGGCTTTCCCGCGCTTGCGACGACGAGCGCCGGCTATG
CGTATTCGCGCGGCCTGCCCGACAACGCGATCGGCCGCGACGCGATGCTCGAGCACATTGCCGTGATCGCTGCCGCCGGC
GAGTTGCCGGTCAGCGCCGACCTCGAAAACGGTTTTGGCGACGCGCCCGAGATCGTCGCCGAGACGATCCGGCTCGCGGC
GGAAGCGGGTGCTGTCGGCGGGTCGATCGAGGATGCAACCGGCCGTGCCGACGCGCCGATCTATCCGCTCGACGCGGCGG
TCGAACGGATCTCGGCCGCCGCCGCGGCGGCACGCGCGCTGCCGTTTCCGTTTACGCTGACCGCCCGCTGCGAAAACTAT
CTGCACGGCCGGCGCGATCTCGCGGACACGATCGCTCGCCTCGTCGCGTATCGCGACGCGGGCGCCGACGTGCTCTATGC
GCCGGGCTTGACCGATCCGGCCGACATCGAGGCCGTGACGCGGTCCGTGCAGGCGCCCGTCAACGTCGTGATGGGATTGC
AGGGCGCGGCGCTGAGCGTCGATGCGCTGGCGGCGCTCGGCGTGAAGCGCATCAGCGTCGGCAGCGCGCTTGCGCGTGCC
GCACTCGGTGCGTTCGTGCGCGCGATCGGCGAGATCACGCATGCCGGCACGTTCGAATTCGCCGCGCACGCGGTGCCGTC
GCGCGATCTCGATCGCTGGTTCGCGGCGTCCGGCAATCCGCCCGTCGGATTCGAGCGTTAA

Upstream 100 bases:

>100_bases
TGCGCACGCCGCGCTTGTTTCCGCGACGCTTCAGCGCGCAGCGAAACGTCGTCGCATCGTGCGCGCGTATGCTGACGTCT
CCGATTCCGAGGAGACGACG

Downstream 100 bases:

>100_bases
TCGGTTCGGAAAATCGTTTTCAGCAGAAACTTCATTACCGATAATCGGCGCACATCGCGCGATATTGAAACGATGCGAAT
CGGCGCGGCAGCGTGCTTTC

Product: putative PEP phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MPYAAHQTRHAETFRALHARAGAFVIPNPWDAGTARLLAMAGFPALATTSAGYAYSRGLPDNAIGRDAMLEHIAVIAAAG
ELPVSADLENGFGDAPEIVAETIRLAAEAGAVGGSIEDATGRADAPIYPLDAAVERISAAAAAARALPFPFTLTARCENY
LHGRRDLADTIARLVAYRDAGADVLYAPGLTDPADIEAVTRSVQAPVNVVMGLQGAALSVDALAALGVKRISVGSALARA
ALGAFVRAIGEITHAGTFEFAAHAVPSRDLDRWFAASGNPPVGFER

Sequences:

>Translated_286_residues
MPYAAHQTRHAETFRALHARAGAFVIPNPWDAGTARLLAMAGFPALATTSAGYAYSRGLPDNAIGRDAMLEHIAVIAAAG
ELPVSADLENGFGDAPEIVAETIRLAAEAGAVGGSIEDATGRADAPIYPLDAAVERISAAAAAARALPFPFTLTARCENY
LHGRRDLADTIARLVAYRDAGADVLYAPGLTDPADIEAVTRSVQAPVNVVMGLQGAALSVDALAALGVKRISVGSALARA
ALGAFVRAIGEITHAGTFEFAAHAVPSRDLDRWFAASGNPPVGFER
>Mature_285_residues
PYAAHQTRHAETFRALHARAGAFVIPNPWDAGTARLLAMAGFPALATTSAGYAYSRGLPDNAIGRDAMLEHIAVIAAAGE
LPVSADLENGFGDAPEIVAETIRLAAEAGAVGGSIEDATGRADAPIYPLDAAVERISAAAAAARALPFPFTLTARCENYL
HGRRDLADTIARLVAYRDAGADVLYAPGLTDPADIEAVTRSVQAPVNVVMGLQGAALSVDALAALGVKRISVGSALARAA
LGAFVRAIGEITHAGTFEFAAHAVPSRDLDRWFAASGNPPVGFER

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=202, Percent_Identity=32.6732673267327, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29534; Mature: 29402

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYAAHQTRHAETFRALHARAGAFVIPNPWDAGTARLLAMAGFPALATTSAGYAYSRGLP
CCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCHHHCCCC
DNAIGRDAMLEHIAVIAAAGELPVSADLENGFGDAPEIVAETIRLAAEAGAVGGSIEDAT
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GRADAPIYPLDAAVERISAAAAAARALPFPFTLTARCENYLHGRRDLADTIARLVAYRDA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GADVLYAPGLTDPADIEAVTRSVQAPVNVVMGLQGAALSVDALAALGVKRISVGSALARA
CCCEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALGAFVRAIGEITHAGTFEFAAHAVPSRDLDRWFAASGNPPVGFER
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PYAAHQTRHAETFRALHARAGAFVIPNPWDAGTARLLAMAGFPALATTSAGYAYSRGLP
CCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCHHHCCCC
DNAIGRDAMLEHIAVIAAAGELPVSADLENGFGDAPEIVAETIRLAAEAGAVGGSIEDAT
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GRADAPIYPLDAAVERISAAAAAARALPFPFTLTARCENYLHGRRDLADTIARLVAYRDA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHCC
GADVLYAPGLTDPADIEAVTRSVQAPVNVVMGLQGAALSVDALAALGVKRISVGSALARA
CCCEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALGAFVRAIGEITHAGTFEFAAHAVPSRDLDRWFAASGNPPVGFER
HHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]