Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 189353555

GI number: 189353555

Start: 1793006

End: 1794295

Strand: Reverse

Name: puuB [H]

Synonym: BMULJ_04799

Alternate gene names: 189353555

Gene position: 1794295-1793006 (Counterclockwise)

Preceding gene: 189353556

Following gene: 189353554

Centisome position: 72.55

GC content: 72.48

Gene sequence:

>1290_bases
ATGTTCGTGCAGTCCGACCGTCACGTCGCAAGTTATTACGCCGGCACGTATCCGGCGCCGATCCCGCGTCGGCCGGCACT
GGACGAGCACGTCCGCGCCGACGTGCTCGTCGTCGGCGCCGGCTTCAGCGGGCTGCATACCGCGTTGCGGCTCGCGCTGG
CCGGCCTGCGTGTCGTCGTGCTCGAAGCGAGCCGTGTCGCGTGGGCGGCGTCGGGGCGCAACGGCGGGCAGGCGCTGCTC
GGCTGGTCGTGCGACATGCCGCCGCTCGAGGCCGCGCTCGGCCGCGACGGCGCGCGCCTGCTATGGGACAGCATGCGCTG
GGCCGCGGCCGAGGTGCGCGAGCTGCCGGCCCGGCACGGCTTCGACGTCGACTACCGGCCCGGCAGCCTGTGGGCCGCCG
TGCGGCCGCGCCGCGTCGCGATGCTCGCACAGGCGCGTGACGAGGCGGCCGAGCGCTGGGGCTACGACCGTCTGCGCGTC
ATCGAGCGACACGCGTTGCGCGACTGGATCGGCAGCACGCGCTACCAGGCGGCGCTCTACGATCCCGAGGCCGGACACCT
GAATCCGCTGAAGCTCGCGCTCGGCGTCGCGCATGCGCTCGAACGCGCGGGCGGCCGGATCTTCGAGCAGAGCCGCGTGC
TCGACTATCGCGAGACGGACAGCGGCTACGTTGCGCGCACGGAGACCGGCGAAGTACAGGCGGACTGCCTCGTGCTCGCG
TGCAACGCGTATGTGGATACGCTCGATCGCGAGCTGTCGCGCCGGCTGCTGCCGGTCGGCACGTACCAGATCGCGACCGC
GCCGCTCGCGCCCGACGTCGCGCGCACGCTGCTGCCGCAGAACAGCTGCGTGATCGACAACCAGTTCGTCCCCGACTATT
TCCGGCTGAGCCCCGACGGGCGGCTGCTGTTCGGCGGCGGCTGCACCTATCTCGGCGGCATTCCGGCCGACATCGCGGCC
GCCACGCGGCCTCACGTCGAACGCGTGTTTCCGCAGTTGCGCGGCGTGCCGATCGACTATGCATGGGGCGGCCATATCGA
CATCAGCATGCGCCGCACGCCGGACATCGGTCGCGTCGGGCAGCGCTTCTGGCTGCAGGGGTTCTCGGGCCACGGCGTGC
TGCCGACGCTCGCGGGCGCGCGCGCCGTCGCCGACGCGGTGCTCGGCGACGATCGCCTGCTCGCGCAGTATCGTCGCATC
CGCAACCGGCGCTTTCCCGGCGGCGACCGGCTCGCCGCGCCGCTGGAGGCGATCGGCAAGGCTTGGTACCGTCTGCGCGA
CACCGTCTGA

Upstream 100 bases:

>100_bases
CGGACTATTCGCGCGCGCCCCGCCCCAATCGCTTGCTCCCATGAAATTGACGTGCTAGTTTTTCATGAAAATTTAAAGCA
TAAATTTCACGGAGACACGC

Downstream 100 bases:

>100_bases
CCGCATCGGAATTCATCATGAACGAACAGGAAGAGATAGAAAGCCTTGCGATCCTGATCCGCGATCTGCGCAAGCATCGG
AAGGTCACGCTGAGCGAACT

Product: gamma-glutamylputrescine oxidase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MFVQSDRHVASYYAGTYPAPIPRRPALDEHVRADVLVVGAGFSGLHTALRLALAGLRVVVLEASRVAWAASGRNGGQALL
GWSCDMPPLEAALGRDGARLLWDSMRWAAAEVRELPARHGFDVDYRPGSLWAAVRPRRVAMLAQARDEAAERWGYDRLRV
IERHALRDWIGSTRYQAALYDPEAGHLNPLKLALGVAHALERAGGRIFEQSRVLDYRETDSGYVARTETGEVQADCLVLA
CNAYVDTLDRELSRRLLPVGTYQIATAPLAPDVARTLLPQNSCVIDNQFVPDYFRLSPDGRLLFGGGCTYLGGIPADIAA
ATRPHVERVFPQLRGVPIDYAWGGHIDISMRRTPDIGRVGQRFWLQGFSGHGVLPTLAGARAVADAVLGDDRLLAQYRRI
RNRRFPGGDRLAAPLEAIGKAWYRLRDTV

Sequences:

>Translated_429_residues
MFVQSDRHVASYYAGTYPAPIPRRPALDEHVRADVLVVGAGFSGLHTALRLALAGLRVVVLEASRVAWAASGRNGGQALL
GWSCDMPPLEAALGRDGARLLWDSMRWAAAEVRELPARHGFDVDYRPGSLWAAVRPRRVAMLAQARDEAAERWGYDRLRV
IERHALRDWIGSTRYQAALYDPEAGHLNPLKLALGVAHALERAGGRIFEQSRVLDYRETDSGYVARTETGEVQADCLVLA
CNAYVDTLDRELSRRLLPVGTYQIATAPLAPDVARTLLPQNSCVIDNQFVPDYFRLSPDGRLLFGGGCTYLGGIPADIAA
ATRPHVERVFPQLRGVPIDYAWGGHIDISMRRTPDIGRVGQRFWLQGFSGHGVLPTLAGARAVADAVLGDDRLLAQYRRI
RNRRFPGGDRLAAPLEAIGKAWYRLRDTV
>Mature_429_residues
MFVQSDRHVASYYAGTYPAPIPRRPALDEHVRADVLVVGAGFSGLHTALRLALAGLRVVVLEASRVAWAASGRNGGQALL
GWSCDMPPLEAALGRDGARLLWDSMRWAAAEVRELPARHGFDVDYRPGSLWAAVRPRRVAMLAQARDEAAERWGYDRLRV
IERHALRDWIGSTRYQAALYDPEAGHLNPLKLALGVAHALERAGGRIFEQSRVLDYRETDSGYVARTETGEVQADCLVLA
CNAYVDTLDRELSRRLLPVGTYQIATAPLAPDVARTLLPQNSCVIDNQFVPDYFRLSPDGRLLFGGGCTYLGGIPADIAA
ATRPHVERVFPQLRGVPIDYAWGGHIDISMRRTPDIGRVGQRFWLQGFSGHGVLPTLAGARAVADAVLGDDRLLAQYRRI
RNRRFPGGDRLAAPLEAIGKAWYRLRDTV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=40.8983451536643, Blast_Score=318, Evalue=4e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47300; Mature: 47300

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVQSDRHVASYYAGTYPAPIPRRPALDEHVRADVLVVGAGFSGLHTALRLALAGLRVVV
CCCCCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHCCEEEE
LEASRVAWAASGRNGGQALLGWSCDMPPLEAALGRDGARLLWDSMRWAAAEVRELPARHG
EECCCEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCC
FDVDYRPGSLWAAVRPRRVAMLAQARDEAAERWGYDRLRVIERHALRDWIGSTRYQAALY
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEE
DPEAGHLNPLKLALGVAHALERAGGRIFEQSRVLDYRETDSGYVARTETGEVQADCLVLA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCEEECCCCEEEECCCCCCEECEEEEE
CNAYVDTLDRELSRRLLPVGTYQIATAPLAPDVARTLLPQNSCVIDNQFVPDYFRLSPDG
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCC
RLLFGGGCTYLGGIPADIAAATRPHVERVFPQLRGVPIDYAWGGHIDISMRRTPDIGRVG
CEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHH
QRFWLQGFSGHGVLPTLAGARAVADAVLGDDRLLAQYRRIRNRRFPGGDRLAAPLEAIGK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AWYRLRDTV
HHHHHHCCC
>Mature Secondary Structure
MFVQSDRHVASYYAGTYPAPIPRRPALDEHVRADVLVVGAGFSGLHTALRLALAGLRVVV
CCCCCCCHHHHHHCCCCCCCCCCCCCCHHHCCCCEEEEECCCHHHHHHHHHHHHCCEEEE
LEASRVAWAASGRNGGQALLGWSCDMPPLEAALGRDGARLLWDSMRWAAAEVRELPARHG
EECCCEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHCC
FDVDYRPGSLWAAVRPRRVAMLAQARDEAAERWGYDRLRVIERHALRDWIGSTRYQAALY
CCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEE
DPEAGHLNPLKLALGVAHALERAGGRIFEQSRVLDYRETDSGYVARTETGEVQADCLVLA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCEEECCCCEEEECCCCCCEECEEEEE
CNAYVDTLDRELSRRLLPVGTYQIATAPLAPDVARTLLPQNSCVIDNQFVPDYFRLSPDG
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCC
RLLFGGGCTYLGGIPADIAAATRPHVERVFPQLRGVPIDYAWGGHIDISMRRTPDIGRVG
CEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCHHHHH
QRFWLQGFSGHGVLPTLAGARAVADAVLGDDRLLAQYRRIRNRRFPGGDRLAAPLEAIGK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AWYRLRDTV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]