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The map label for this gene is clpB [H]

Identifier: 189353355

GI number: 189353355

Start: 1600900

End: 1603581

Strand: Reverse

Name: clpB [H]

Synonym: BMULJ_04595

Alternate gene names: 189353355

Gene position: 1603581-1600900 (Counterclockwise)

Preceding gene: 189353361

Following gene: 189353354

Centisome position: 64.84

GC content: 68.16

Gene sequence:

>2682_bases
ATGAACATTTCACGCCAGACCTTGTTCGGCAAGCTCGGGGTCGAGCTCTACCGCGGAATCGAATCGGCGACGCGTTTCTG
CAAGCTGCGCGGGAACCCGTTCGTCGAACTCGTCCACTGGCTGCATCAACTGCTCCAGCAGCCGGACGGCGACCTGCAGC
GGATCGTGCGCCATGCGGATATCGACCGCGATGCGCTCGAGCGCGATCTGGCGCGGGCGTTGGCTGCGCTGCCGGCCGGC
GCGAGTTCGATCAGCGATTTCTCCTGGCACATCGAAACGGCCATCGAGCGCGGCTGGGTGCTGGCGACCCTCGGCCATGG
CGATTGTCGCGTGCGCGGCGCATGGCTGATCGCAGCGCTGGTCTCGACACCGGAACTGCGGCGCGTGCTCCTCGCCATTT
CCGCCGAGTTCGGCAAGATCCCGGTGGCGAGTCTTGGCGACGTGCTTCCCGCGTGGATCGACGGGTCGCCGGAGGCGTCG
GACGAGCCGTACGATCGCAGCAAGCTCGCGCCGGCGCTGCCGGGCGAGGCTTCCGGTGCCGTGTCGGACGCGCCGAAGGG
CAACGCGCTCGAGCGGTATTGCCTCGATCTCACCGCGCGAGCACGCGCCGGCGAAATCGATCCCGTTATCGGCCGCGAGC
TGGAAATCCGCACCATGGTCGACGTGCTGCTGCGTCGGCGGCAGAACAATCCGCTGATCACCGGCGAGGCCGGTGTCGGC
AAGACCGCGATGGTGGAGGGGTTGGCGCATGCGATCGCCGCGGGCAACGTGCCGCCGAATCTGGCCGACGTGCGATTGCT
GAGCCTCGATGTCGGCGCGCTGCTGGCGGGCGCCAGCATGAAGGGCGAATTCGAGGCGCGCTTGAAGGGCGTCCTCGAGG
CCGCAGCACGCTCCGCGGCGCCCATCATCCTGTTCGTCGACGAAATCCATACGCTGATCGGCGCAGGCGGGCAAGCCGGC
ACCGGCGACGCCGCGAACCTCCTGAAACCGGCGCTCGCGCGCGGCACGATCCGCACTATCGGCGCGACGACCTGGGCCGA
ATACAAGCGGCATATCGAGAAGGATCCCGCGCTGACGCGGCGCTTTCAGGTGTTGCAGGTCGCGGAGCCGTCCGAAGCGG
CGGCGGCCGACATGGTGCGCGGCCTCGCGTCGACGTTTTCGTCGCATCACGGCGTCGTCGTGCGGGACGAAGCGATCCGC
GCAGCAGTAACGCTGTCGCATCGTTACATTCCGTCGCGTCAATTGCCGGACAAGGCGATCAGCCTGCTCGACACCGCATG
CGCGCGCGTCGCGCTGTCGCAGCACGCGACGCCGCGCGAACTCGACGACGTGCGGCAGCGCCTGGCTGCCGCGCGCACCG
AAGAAACGCTGCTGGTACAGGAGGCCCGTATCGGTCTCGAAGCGGACAAGGCGCTGCATCGCGTGCGCACGCGCATCGAC
GCACTGGCGACGGAAGAAGCGGCACTCGCGGATCGCTGGCGCGCACAGGCGCAGGCCGCGCAGGCGTTGCTTTCCGCGCG
CGAAGCCGCGATCGCGGACGAGCGCGATTCGCCGGTATCGCTCGACAGCTTGCGTGAGCTCGAACGGACGCTGGTCGCGC
TGCAGGGCGACGCGCCGCTCGTCTTTCCCGAAGTGGACGCCGCCATCGTCGCCGAGATCGTATCGGACTGGACCGGCATC
CCGGTCGGTCGCATGGTCACCGACGAGATCGTCGCCGTGCGCCGCCTTCCGGAAACGCTCGCGGTCCGCGTGATCGGGCA
GAGCGATGCGCTGAACCTGATCGGCGAACGTGTGCAGACCGCGCGGTCGGGGCTGACCGATCCGAACAAGCCGCTGGGCG
TGTTTCTGCTGGCGGGGCCGTCCGGCGTCGGCAAGACCGAGACGGCGCTGGCGCTGGCCGAGGCGCTATACGGCGGCGAG
CAGAACCTGATCACGATCAACATGAGCGAGTACCAGGAGGCCCACACCGTGTCGGGCCTGAAAGGCGCGCCGCCCGGCTA
TGTCGGCTACGGCGAGGGCGGTGTGCTGACCGAAGCGGTGCGCCGGCGGCCCTACGCGGTCGTGTTGCTCGACGAGATCG
AAAAGGCGCATCGCGACGTGCACGAAATGTTCTTCCAGGTGTTCGACAAGGGCTACATGGAAGACGGCGACGGTCGCCAT
ATCGACTTCCGCCATACCACCATCCTGCTGACGAGCAACGTCGGCGCCGAGCTTGGCGCGAGCCTGTGTGCCGATCCGGC
GCTGGCACCGGATCTCGACGGCCTGCGCGACGCGCTGGCACCGGAACTGCTGAAGGTATTCCCGGCGGCGTTCATGGGGC
GTGTGACCGTTGTGCCGTACCGGCCGCTCGCCGCCGACGCGCTCGCGAGCATCGTCCGCCTGCATCTGGACCGCGTCGCG
AAACGCATGGCACACACCCACGACATCGCGCTCGTCTGCGACGACGCAGTCGTGCATTACATCGTCGGCCGTTGCCTCGT
TCAGGAGACGGGCGCGCGCGTGCTGATCGGCTTCATCGAACAGCATGTGCTGCCGCGCCTTTCCGCGCTCTGGCTCGATG
CGTTCGCATCGCGCGTGCCGCTCGCGCGCATCGCGATCGGCGTAACCGATTCCTCCGCGTCGCCCGCACAGGCGCTGACG
TTCGAGGCCACCCCTTCTCATCCTCTCGAGCCGCGTCGTTGA

Upstream 100 bases:

>100_bases
TCGAGCGATCGGGATCGCGTCCGGCGTGGCGACCATCAAGGATTCCTCATCGCTGTGACGGATTCGCTCGCCACACGCTA
ACGAACAAAACCGGACCACC

Downstream 100 bases:

>100_bases
GCTCGATATCCGTCAACCCCTGTCAGGAGACCGTTCATGTCCGCTAGCAATTCGCAGAAGTTCATCGCGCGCAATCGTGC
GCCGCGTGTGCAGATCGAGT

Product: ATP-dependent Clp protease ATP-binding subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 893; Mature: 893

Protein sequence:

>893_residues
MNISRQTLFGKLGVELYRGIESATRFCKLRGNPFVELVHWLHQLLQQPDGDLQRIVRHADIDRDALERDLARALAALPAG
ASSISDFSWHIETAIERGWVLATLGHGDCRVRGAWLIAALVSTPELRRVLLAISAEFGKIPVASLGDVLPAWIDGSPEAS
DEPYDRSKLAPALPGEASGAVSDAPKGNALERYCLDLTARARAGEIDPVIGRELEIRTMVDVLLRRRQNNPLITGEAGVG
KTAMVEGLAHAIAAGNVPPNLADVRLLSLDVGALLAGASMKGEFEARLKGVLEAAARSAAPIILFVDEIHTLIGAGGQAG
TGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTRRFQVLQVAEPSEAAAADMVRGLASTFSSHHGVVVRDEAIR
AAVTLSHRYIPSRQLPDKAISLLDTACARVALSQHATPRELDDVRQRLAAARTEETLLVQEARIGLEADKALHRVRTRID
ALATEEAALADRWRAQAQAAQALLSAREAAIADERDSPVSLDSLRELERTLVALQGDAPLVFPEVDAAIVAEIVSDWTGI
PVGRMVTDEIVAVRRLPETLAVRVIGQSDALNLIGERVQTARSGLTDPNKPLGVFLLAGPSGVGKTETALALAEALYGGE
QNLITINMSEYQEAHTVSGLKGAPPGYVGYGEGGVLTEAVRRRPYAVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRH
IDFRHTTILLTSNVGAELGASLCADPALAPDLDGLRDALAPELLKVFPAAFMGRVTVVPYRPLAADALASIVRLHLDRVA
KRMAHTHDIALVCDDAVVHYIVGRCLVQETGARVLIGFIEQHVLPRLSALWLDAFASRVPLARIAIGVTDSSASPAQALT
FEATPSHPLEPRR

Sequences:

>Translated_893_residues
MNISRQTLFGKLGVELYRGIESATRFCKLRGNPFVELVHWLHQLLQQPDGDLQRIVRHADIDRDALERDLARALAALPAG
ASSISDFSWHIETAIERGWVLATLGHGDCRVRGAWLIAALVSTPELRRVLLAISAEFGKIPVASLGDVLPAWIDGSPEAS
DEPYDRSKLAPALPGEASGAVSDAPKGNALERYCLDLTARARAGEIDPVIGRELEIRTMVDVLLRRRQNNPLITGEAGVG
KTAMVEGLAHAIAAGNVPPNLADVRLLSLDVGALLAGASMKGEFEARLKGVLEAAARSAAPIILFVDEIHTLIGAGGQAG
TGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTRRFQVLQVAEPSEAAAADMVRGLASTFSSHHGVVVRDEAIR
AAVTLSHRYIPSRQLPDKAISLLDTACARVALSQHATPRELDDVRQRLAAARTEETLLVQEARIGLEADKALHRVRTRID
ALATEEAALADRWRAQAQAAQALLSAREAAIADERDSPVSLDSLRELERTLVALQGDAPLVFPEVDAAIVAEIVSDWTGI
PVGRMVTDEIVAVRRLPETLAVRVIGQSDALNLIGERVQTARSGLTDPNKPLGVFLLAGPSGVGKTETALALAEALYGGE
QNLITINMSEYQEAHTVSGLKGAPPGYVGYGEGGVLTEAVRRRPYAVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRH
IDFRHTTILLTSNVGAELGASLCADPALAPDLDGLRDALAPELLKVFPAAFMGRVTVVPYRPLAADALASIVRLHLDRVA
KRMAHTHDIALVCDDAVVHYIVGRCLVQETGARVLIGFIEQHVLPRLSALWLDAFASRVPLARIAIGVTDSSASPAQALT
FEATPSHPLEPRR
>Mature_893_residues
MNISRQTLFGKLGVELYRGIESATRFCKLRGNPFVELVHWLHQLLQQPDGDLQRIVRHADIDRDALERDLARALAALPAG
ASSISDFSWHIETAIERGWVLATLGHGDCRVRGAWLIAALVSTPELRRVLLAISAEFGKIPVASLGDVLPAWIDGSPEAS
DEPYDRSKLAPALPGEASGAVSDAPKGNALERYCLDLTARARAGEIDPVIGRELEIRTMVDVLLRRRQNNPLITGEAGVG
KTAMVEGLAHAIAAGNVPPNLADVRLLSLDVGALLAGASMKGEFEARLKGVLEAAARSAAPIILFVDEIHTLIGAGGQAG
TGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTRRFQVLQVAEPSEAAAADMVRGLASTFSSHHGVVVRDEAIR
AAVTLSHRYIPSRQLPDKAISLLDTACARVALSQHATPRELDDVRQRLAAARTEETLLVQEARIGLEADKALHRVRTRID
ALATEEAALADRWRAQAQAAQALLSAREAAIADERDSPVSLDSLRELERTLVALQGDAPLVFPEVDAAIVAEIVSDWTGI
PVGRMVTDEIVAVRRLPETLAVRVIGQSDALNLIGERVQTARSGLTDPNKPLGVFLLAGPSGVGKTETALALAEALYGGE
QNLITINMSEYQEAHTVSGLKGAPPGYVGYGEGGVLTEAVRRRPYAVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRH
IDFRHTTILLTSNVGAELGASLCADPALAPDLDGLRDALAPELLKVFPAAFMGRVTVVPYRPLAADALASIVRLHLDRVA
KRMAHTHDIALVCDDAVVHYIVGRCLVQETGARVLIGFIEQHVLPRLSALWLDAFASRVPLARIAIGVTDSSASPAQALT
FEATPSHPLEPRR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=303, Percent_Identity=32.6732673267327, Blast_Score=149, Evalue=1e-35,
Organism=Escherichia coli, GI1788943, Length=807, Percent_Identity=39.2812887236679, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1787109, Length=405, Percent_Identity=38.0246913580247, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6320464, Length=689, Percent_Identity=38.7518142235123, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323002, Length=698, Percent_Identity=34.9570200573066, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR017729
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96017; Mature: 96017

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00213 LIPOCALIN ; PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISRQTLFGKLGVELYRGIESATRFCKLRGNPFVELVHWLHQLLQQPDGDLQRIVRHAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
IDRDALERDLARALAALPAGASSISDFSWHIETAIERGWVLATLGHGDCRVRGAWLIAAL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHH
VSTPELRRVLLAISAEFGKIPVASLGDVLPAWIDGSPEASDEPYDRSKLAPALPGEASGA
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCC
VSDAPKGNALERYCLDLTARARAGEIDPVIGRELEIRTMVDVLLRRRQNNPLITGEAGVG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCC
KTAMVEGLAHAIAAGNVPPNLADVRLLSLDVGALLAGASMKGEFEARLKGVLEAAARSAA
HHHHHHHHHHHHHCCCCCCCHHHEEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
PIILFVDEIHTLIGAGGQAGTGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTR
CEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHH
RFQVLQVAEPSEAAAADMVRGLASTFSSHHGVVVRDEAIRAAVTLSHRYIPSRQLPDKAI
HHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
SLLDTACARVALSQHATPRELDDVRQRLAAARTEETLLVQEARIGLEADKALHRVRTRID
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALATEEAALADRWRAQAQAAQALLSAREAAIADERDSPVSLDSLRELERTLVALQGDAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
VFPEVDAAIVAEIVSDWTGIPVGRMVTDEIVAVRRLPETLAVRVIGQSDALNLIGERVQT
EECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHHH
ARSGLTDPNKPLGVFLLAGPSGVGKTETALALAEALYGGEQNLITINMSEYQEAHTVSGL
HHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCC
KGAPPGYVGYGEGGVLTEAVRRRPYAVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRH
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
IDFRHTTILLTSNVGAELGASLCADPALAPDLDGLRDALAPELLKVFPAAFMGRVTVVPY
EEEEEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
RPLAADALASIVRLHLDRVAKRMAHTHDIALVCDDAVVHYIVGRCLVQETGARVLIGFIE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
QHVLPRLSALWLDAFASRVPLARIAIGVTDSSASPAQALTFEATPSHPLEPRR
HHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNISRQTLFGKLGVELYRGIESATRFCKLRGNPFVELVHWLHQLLQQPDGDLQRIVRHAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
IDRDALERDLARALAALPAGASSISDFSWHIETAIERGWVLATLGHGDCRVRGAWLIAAL
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHHH
VSTPELRRVLLAISAEFGKIPVASLGDVLPAWIDGSPEASDEPYDRSKLAPALPGEASGA
HCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCC
VSDAPKGNALERYCLDLTARARAGEIDPVIGRELEIRTMVDVLLRRRQNNPLITGEAGVG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCC
KTAMVEGLAHAIAAGNVPPNLADVRLLSLDVGALLAGASMKGEFEARLKGVLEAAARSAA
HHHHHHHHHHHHHCCCCCCCHHHEEEEEECHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
PIILFVDEIHTLIGAGGQAGTGDAANLLKPALARGTIRTIGATTWAEYKRHIEKDPALTR
CEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHH
RFQVLQVAEPSEAAAADMVRGLASTFSSHHGVVVRDEAIRAAVTLSHRYIPSRQLPDKAI
HHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
SLLDTACARVALSQHATPRELDDVRQRLAAARTEETLLVQEARIGLEADKALHRVRTRID
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALATEEAALADRWRAQAQAAQALLSAREAAIADERDSPVSLDSLRELERTLVALQGDAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
VFPEVDAAIVAEIVSDWTGIPVGRMVTDEIVAVRRLPETLAVRVIGQSDALNLIGERVQT
EECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCHHHHHHHHHHHH
ARSGLTDPNKPLGVFLLAGPSGVGKTETALALAEALYGGEQNLITINMSEYQEAHTVSGL
HHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCC
KGAPPGYVGYGEGGVLTEAVRRRPYAVVLLDEIEKAHRDVHEMFFQVFDKGYMEDGDGRH
CCCCCCCCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
IDFRHTTILLTSNVGAELGASLCADPALAPDLDGLRDALAPELLKVFPAAFMGRVTVVPY
EEEEEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
RPLAADALASIVRLHLDRVAKRMAHTHDIALVCDDAVVHYIVGRCLVQETGARVLIGFIE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHH
QHVLPRLSALWLDAFASRVPLARIAIGVTDSSASPAQALTFEATPSHPLEPRR
HHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10986262 [H]