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The map label for this gene is amiE

Identifier: 189353261

GI number: 189353261

Start: 1496808

End: 1498292

Strand: Reverse

Name: amiE

Synonym: BMULJ_04501

Alternate gene names: NA

Gene position: 1498292-1496808 (Counterclockwise)

Preceding gene: 189353266

Following gene: 189353259

Centisome position: 60.58

GC content: 70.77

Gene sequence:

>1485_bases
ATGCCACACGGCGCCCCGTCGCTTCTCGCTCCCGCATCCGTTCCCGTCGATCCGATCGTGCGCCTGTCGGCCGGCGAACT
CGCCTCGGCGATCCGCAGCAAGGCCGTGTCGTGCGTCGAGACGATGCGCGCGTATCTCGATCACATCGAGCGCGTGAACG
GCGCCGTGAACGCGATCGTCGCGCTGCGCGAGCGCGACGCGCTGCTCGCCGAAGCCGCGGACAAGGACGCGGCGCTCGCG
CGCGGCGAGTATCACGGCTGGCTGCACGGGATGCCGCAGGCGCCGAAGGATCTCGCGATGACGAAGGGGCTCGTGACGAC
CTGCGGCTCGCGGATCTTCAGCGATCACGTGCCGCAGGCCGATTCGGTCGGCGTCGCGCGCATGCGCGCGGCCGGCGCGA
TCTTTATCGGCAAGACCAATACGCCGGAGTTCGGCCTCGGTTCGCATACGTTCAACGACGTATACGGCGCGACGCGCAAT
CCGTACGACCTGACGAAGAGCGCAGGCGGCAGCAGCGGCGGCACGGCGGCCGCGCTCGCGTCGCGGATGCTGCCGGTCGC
GGACGGCAGCGACTTCGGCGGCTCGCTGCGCAATCCGGCCGCGTTCTGCAACATCTACGGCTTCCGGCCGTCGCAGGGCC
GCGTGCCGCGCTGGCCGGGCGTCGACGTCTACATGCAGCAGCTCGGCATCGAAGGGCCGATGGGCCGCACGGTCGGCGAC
GTCGCGCAACTGCTCGCGATCCAGGCCGGCTACGACCCGAACGATCCGTTGTCGCTCGCCGACGATCCGGCCGTATTCGC
GCAGCCGCTCGAGACGGACGTGAAGGGCACGCGCATCGCCTGGGTCGGCGACTGGAACGGTTATCTCGCGACGGAGCCCG
GCGTGCTCGCGCAGTGCGAGCAGGGGCTCGCGACGCTGCGCGACATCGGCTGCGACGTCGATGCGGCGTTGCCCGCGTTC
GTGCCGGAGCGCATCTGGCGCCTGTGGCTCGCGCATCGGCATCTGCTGTCGGGCGGCGGGCTGCTCGCGCACTATCGCGA
CCCGGCGCGCCGCGCGCTGCTGAAGCCGGAAGCGATCTACGAGGTCGAAGGGCTGCTCGCGATGCAGGGCGCCGCGGTGT
TCGACGCAAGCGTCGAGCGCACCGCGTGGCATCAGGCCGTGCTGCGCTTTTTCGAGCGCTACGACTTCATCGCGGCGCCG
ACCGCGCAGGTCTTTCCGTTCGACGTCGAGCAGCGCTGGCCGAAGGAAATCGCGGGCCGCGCGATGGACACCTATCATCG
CTGGATGGAAACGGTCGTCCCGTGGACGCTGGCCGGCTGCCCGGTGATCAGCGTGCCGGTCGGCTTCAACGAGGCGGGGC
TGCCGATGGGGATGCAGCTGATCGGGCGGCCGCGCGCCGATCTCGCCGTGCTGCAGCTGGCGCGCGGCTACGAACGCGCG
GCCGACTGGGTGGATCGTCGGCTGCCGGCGTGGATGGCCGCGTGA

Upstream 100 bases:

>100_bases
CGCGTAGAGTAGAGCGTCGACGACCGGCGCGCCAGCGCGCGCCGCGACGCGCGGCCGTCACGATACGGCCCGGCTTCCCA
TTCGATTTCAGGAGACCCGC

Downstream 100 bases:

>100_bases
CGCGACGACGCGGTTCGCGGCCGGCCGCACGCAGGCCGCACCGCGCGTGCGCGCAGCCCGCCGTCAGAACGTGATGGTCG
TGCGCACGCCGAGCACGAAC

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPHGAPSLLAPASVPVDPIVRLSAGELASAIRSKAVSCVETMRAYLDHIERVNGAVNAIVALRERDALLAEAADKDAALA
RGEYHGWLHGMPQAPKDLAMTKGLVTTCGSRIFSDHVPQADSVGVARMRAAGAIFIGKTNTPEFGLGSHTFNDVYGATRN
PYDLTKSAGGSSGGTAAALASRMLPVADGSDFGGSLRNPAAFCNIYGFRPSQGRVPRWPGVDVYMQQLGIEGPMGRTVGD
VAQLLAIQAGYDPNDPLSLADDPAVFAQPLETDVKGTRIAWVGDWNGYLATEPGVLAQCEQGLATLRDIGCDVDAALPAF
VPERIWRLWLAHRHLLSGGGLLAHYRDPARRALLKPEAIYEVEGLLAMQGAAVFDASVERTAWHQAVLRFFERYDFIAAP
TAQVFPFDVEQRWPKEIAGRAMDTYHRWMETVVPWTLAGCPVISVPVGFNEAGLPMGMQLIGRPRADLAVLQLARGYERA
ADWVDRRLPAWMAA

Sequences:

>Translated_494_residues
MPHGAPSLLAPASVPVDPIVRLSAGELASAIRSKAVSCVETMRAYLDHIERVNGAVNAIVALRERDALLAEAADKDAALA
RGEYHGWLHGMPQAPKDLAMTKGLVTTCGSRIFSDHVPQADSVGVARMRAAGAIFIGKTNTPEFGLGSHTFNDVYGATRN
PYDLTKSAGGSSGGTAAALASRMLPVADGSDFGGSLRNPAAFCNIYGFRPSQGRVPRWPGVDVYMQQLGIEGPMGRTVGD
VAQLLAIQAGYDPNDPLSLADDPAVFAQPLETDVKGTRIAWVGDWNGYLATEPGVLAQCEQGLATLRDIGCDVDAALPAF
VPERIWRLWLAHRHLLSGGGLLAHYRDPARRALLKPEAIYEVEGLLAMQGAAVFDASVERTAWHQAVLRFFERYDFIAAP
TAQVFPFDVEQRWPKEIAGRAMDTYHRWMETVVPWTLAGCPVISVPVGFNEAGLPMGMQLIGRPRADLAVLQLARGYERA
ADWVDRRLPAWMAA
>Mature_493_residues
PHGAPSLLAPASVPVDPIVRLSAGELASAIRSKAVSCVETMRAYLDHIERVNGAVNAIVALRERDALLAEAADKDAALAR
GEYHGWLHGMPQAPKDLAMTKGLVTTCGSRIFSDHVPQADSVGVARMRAAGAIFIGKTNTPEFGLGSHTFNDVYGATRNP
YDLTKSAGGSSGGTAAALASRMLPVADGSDFGGSLRNPAAFCNIYGFRPSQGRVPRWPGVDVYMQQLGIEGPMGRTVGDV
AQLLAIQAGYDPNDPLSLADDPAVFAQPLETDVKGTRIAWVGDWNGYLATEPGVLAQCEQGLATLRDIGCDVDAALPAFV
PERIWRLWLAHRHLLSGGGLLAHYRDPARRALLKPEAIYEVEGLLAMQGAAVFDASVERTAWHQAVLRFFERYDFIAAPT
AQVFPFDVEQRWPKEIAGRAMDTYHRWMETVVPWTLAGCPVISVPVGFNEAGLPMGMQLIGRPRADLAVLQLARGYERAA
DWVDRRLPAWMAA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=532, Percent_Identity=27.8195488721804, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI222831590, Length=259, Percent_Identity=32.4324324324324, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI166795287, Length=322, Percent_Identity=27.0186335403727, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=26.8292682926829, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17537465, Length=255, Percent_Identity=31.7647058823529, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71990152, Length=308, Percent_Identity=27.9220779220779, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=297, Percent_Identity=28.2828282828283, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=298, Percent_Identity=28.5234899328859, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=315, Percent_Identity=27.6190476190476, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=323, Percent_Identity=27.5541795665635, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=199, Percent_Identity=29.1457286432161, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=401, Percent_Identity=28.1795511221945, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=176, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=28.6343612334802, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=25.9634888438134, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=25.9634888438134, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=25.9634888438134, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356731, Length=513, Percent_Identity=27.4853801169591, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=25.8893280632411, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648113, Length=452, Percent_Identity=28.0973451327434, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=252, Percent_Identity=36.1111111111111, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19922090, Length=252, Percent_Identity=36.1111111111111, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652981, Length=252, Percent_Identity=36.1111111111111, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652983, Length=252, Percent_Identity=36.1111111111111, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078093, Length=263, Percent_Identity=31.1787072243346, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.1798561151079, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.1798561151079, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 53257; Mature: 53126

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHGAPSLLAPASVPVDPIVRLSAGELASAIRSKAVSCVETMRAYLDHIERVNGAVNAIV
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRERDALLAEAADKDAALARGEYHGWLHGMPQAPKDLAMTKGLVTTCGSRIFSDHVPQA
HHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DSVGVARMRAAGAIFIGKTNTPEFGLGSHTFNDVYGATRNPYDLTKSAGGSSGGTAAALA
CCCCHHHHHHCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHHHHH
SRMLPVADGSDFGGSLRNPAAFCNIYGFRPSQGRVPRWPGVDVYMQQLGIEGPMGRTVGD
HHCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHH
VAQLLAIQAGYDPNDPLSLADDPAVFAQPLETDVKGTRIAWVGDWNGYLATEPGVLAQCE
HHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHCCCCCCEEEEEECCCCEEECCCCHHHHHH
QGLATLRDIGCDVDAALPAFVPERIWRLWLAHRHLLSGGGLLAHYRDPARRALLKPEAIY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHH
EVEGLLAMQGAAVFDASVERTAWHQAVLRFFERYDFIAAPTAQVFPFDVEQRWPKEIAGR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHH
AMDTYHRWMETVVPWTLAGCPVISVPVGFNEAGLPMGMQLIGRPRADLAVLQLARGYERA
HHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHH
ADWVDRRLPAWMAA
HHHHHHHCCHHHCC
>Mature Secondary Structure 
PHGAPSLLAPASVPVDPIVRLSAGELASAIRSKAVSCVETMRAYLDHIERVNGAVNAIV
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRERDALLAEAADKDAALARGEYHGWLHGMPQAPKDLAMTKGLVTTCGSRIFSDHVPQA
HHHHHHHHHHHHCCCHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
DSVGVARMRAAGAIFIGKTNTPEFGLGSHTFNDVYGATRNPYDLTKSAGGSSGGTAAALA
CCCCHHHHHHCCEEEEECCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCCCCCHHHHHH
SRMLPVADGSDFGGSLRNPAAFCNIYGFRPSQGRVPRWPGVDVYMQQLGIEGPMGRTVGD
HHCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHH
VAQLLAIQAGYDPNDPLSLADDPAVFAQPLETDVKGTRIAWVGDWNGYLATEPGVLAQCE
HHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHCCCCCCEEEEEECCCCEEECCCCHHHHHH
QGLATLRDIGCDVDAALPAFVPERIWRLWLAHRHLLSGGGLLAHYRDPARRALLKPEAIY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCHHHH
EVEGLLAMQGAAVFDASVERTAWHQAVLRFFERYDFIAAPTAQVFPFDVEQRWPKEIAGR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCHHHHHHH
AMDTYHRWMETVVPWTLAGCPVISVPVGFNEAGLPMGMQLIGRPRADLAVLQLARGYERA
HHHHHHHHHHHHHCHHHCCCCEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHH
ADWVDRRLPAWMAA
HHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]