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The map label for this gene is alsR [H]

Identifier: 189353192

GI number: 189353192

Start: 1419268

End: 1420155

Strand: Reverse

Name: alsR [H]

Synonym: BMULJ_04428

Alternate gene names: 189353192

Gene position: 1420155-1419268 (Counterclockwise)

Preceding gene: 189353199

Following gene: 189353191

Centisome position: 57.42

GC content: 69.93

Gene sequence:

>888_bases
ATGGAACTGAAGCTGCTGAGAACGTTTCTGACCGTCACCGAGCTGAGCCATTTCAGTCGTGCGGCCGATGCGCTGCATAT
GAGCCAGCCGGCGCTGAGCAAGCAGATCGGCGCGCTCGAAGCGAGCCTCGGCGGCAAGCTGTTCGAGCGCGGGCGGCATG
GCGCGGAGCTGACGCCGTTCGGCGAGCGCTTCCTGCCCGATGCAAAGGCGCTCGTGCGCGATGCCGACGAGATCGTTGCG
CGCGCAAGGGAAGCGGCGAGGGGGCAGCGCGGCCATCTGCGGCTCGGCATCTGTTTGTCGGTGCTGACGCTGGTGCCGCG
GCTCGTGGCGGAGTTCCGGCGGCTGAATCCCGGCATCGCCGTGACGCTGAGCGACCTGTCGTCGGCCGAGCAGACGCGCC
GCTTGCGCGCCGGCAAGCTCGACGCCGGTTTTCTGCGGCTGCCGGCCGACGAAGGATTGTCGTCGTGCCGCGTGATCGAC
GAGTCGCTCGCGCTCGCGGTGCCGCCGCAGCTCGGCTTCAAGCGCGTGCCGGCCGATCTCGACGTGCTGAACGAACTCGG
CTTCATCGCGTTGCAGCGCGCACGCGGGGCCGGGCTCGCCGCGCAGATCGATCGATGGTGCATGGCGCGCCGTTTCGTGC
CGCACGTGACGCAGCAGGCGGAAGACGTGCAGTCGGTGCTGACGTCGGTGGCGGCCGGCGTCGGCGTCGCGTTCATCCCG
TCGGGCGCGCAGTATCTGCTGCGCGACGCGACGGTGCTGCCGCTCGACGGCCGCGACGCGAAATGGCGCGTCGGGCTCGC
GTGGCTATCCGGGCGCGACGATCCCGTGACAACGCGTTTCGTCACGTTCATGCGCGCGGCGATTCAGCCGCCGCGCGGCC
GCGTATGA

Upstream 100 bases:

>100_bases
AGGTCATGACGGGATAGTCCGGAAAGGGATTGCCTCCATTGAACGCCATGTCATTCAATCAGTAAAATGAATATTTCGAT
CGTTTCGATTCCCTCCAAGA

Downstream 100 bases:

>100_bases
ACGAAGCGGTATAGTGTGCGCGACCGGCGCGAACCGGCCGGTCGCCTGCCGAACGACTTTGCCGGCCGCGCCGCCGGTCA
CGAACCGATGACCTCCGAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELKLLRTFLTVTELSHFSRAADALHMSQPALSKQIGALEASLGGKLFERGRHGAELTPFGERFLPDAKALVRDADEIVA
RAREAARGQRGHLRLGICLSVLTLVPRLVAEFRRLNPGIAVTLSDLSSAEQTRRLRAGKLDAGFLRLPADEGLSSCRVID
ESLALAVPPQLGFKRVPADLDVLNELGFIALQRARGAGLAAQIDRWCMARRFVPHVTQQAEDVQSVLTSVAAGVGVAFIP
SGAQYLLRDATVLPLDGRDAKWRVGLAWLSGRDDPVTTRFVTFMRAAIQPPRGRV

Sequences:

>Translated_295_residues
MELKLLRTFLTVTELSHFSRAADALHMSQPALSKQIGALEASLGGKLFERGRHGAELTPFGERFLPDAKALVRDADEIVA
RAREAARGQRGHLRLGICLSVLTLVPRLVAEFRRLNPGIAVTLSDLSSAEQTRRLRAGKLDAGFLRLPADEGLSSCRVID
ESLALAVPPQLGFKRVPADLDVLNELGFIALQRARGAGLAAQIDRWCMARRFVPHVTQQAEDVQSVLTSVAAGVGVAFIP
SGAQYLLRDATVLPLDGRDAKWRVGLAWLSGRDDPVTTRFVTFMRAAIQPPRGRV
>Mature_295_residues
MELKLLRTFLTVTELSHFSRAADALHMSQPALSKQIGALEASLGGKLFERGRHGAELTPFGERFLPDAKALVRDADEIVA
RAREAARGQRGHLRLGICLSVLTLVPRLVAEFRRLNPGIAVTLSDLSSAEQTRRLRAGKLDAGFLRLPADEGLSSCRVID
ESLALAVPPQLGFKRVPADLDVLNELGFIALQRARGAGLAAQIDRWCMARRFVPHVTQQAEDVQSVLTSVAAGVGVAFIP
SGAQYLLRDATVLPLDGRDAKWRVGLAWLSGRDDPVTTRFVTFMRAAIQPPRGRV

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=30.1369863013699, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787879, Length=273, Percent_Identity=31.1355311355311, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=32.7868852459016, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081904, Length=177, Percent_Identity=33.8983050847458, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=28.1437125748503, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=30.6034482758621, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=27.3356401384083, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=22.8915662650602, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787806, Length=242, Percent_Identity=27.6859504132231, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=127, Percent_Identity=36.2204724409449, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=72, Percent_Identity=45.8333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082132, Length=251, Percent_Identity=27.4900398406374, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787601, Length=173, Percent_Identity=26.0115606936416, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32112; Mature: 32112

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKLLRTFLTVTELSHFSRAADALHMSQPALSKQIGALEASLGGKLFERGRHGAELTPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCC
GERFLPDAKALVRDADEIVARAREAARGQRGHLRLGICLSVLTLVPRLVAEFRRLNPGIA
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTLSDLSSAEQTRRLRAGKLDAGFLRLPADEGLSSCRVIDESLALAVPPQLGFKRVPADL
EEEHHHHHHHHHHHHHCCCCCCCEEECCHHHCHHHHHHHHHHHHEECCCCCCCCCCCCHH
DVLNELGFIALQRARGAGLAAQIDRWCMARRFVPHVTQQAEDVQSVLTSVAAGVGVAFIP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
SGAQYLLRDATVLPLDGRDAKWRVGLAWLSGRDDPVTTRFVTFMRAAIQPPRGRV
CCHHHHHHCCEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MELKLLRTFLTVTELSHFSRAADALHMSQPALSKQIGALEASLGGKLFERGRHGAELTPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCC
GERFLPDAKALVRDADEIVARAREAARGQRGHLRLGICLSVLTLVPRLVAEFRRLNPGIA
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTLSDLSSAEQTRRLRAGKLDAGFLRLPADEGLSSCRVIDESLALAVPPQLGFKRVPADL
EEEHHHHHHHHHHHHHCCCCCCCEEECCHHHCHHHHHHHHHHHHEECCCCCCCCCCCCHH
DVLNELGFIALQRARGAGLAAQIDRWCMARRFVPHVTQQAEDVQSVLTSVAAGVGVAFIP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
SGAQYLLRDATVLPLDGRDAKWRVGLAWLSGRDDPVTTRFVTFMRAAIQPPRGRV
CCHHHHHHCCEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]