Click here to switch to the map view.

The map label for this gene is amiE [H]

Identifier: 189353184

GI number: 189353184

Start: 1407512

End: 1408858

Strand: Reverse

Name: amiE [H]

Synonym: BMULJ_04420

Alternate gene names: 189353184

Gene position: 1408858-1407512 (Counterclockwise)

Preceding gene: 189353185

Following gene: 189353183

Centisome position: 56.97

GC content: 72.53

Gene sequence:

>1347_bases
ATGAACGGCTTCGATTTCACGTACGACGAACGGCGCGACGCGCGCCCCTGCATCGACCGGCTGCGTGCATGCATTGCCCG
TGCGGCCGCGCATCCGGACGACATCAGCACCGCATGGCTGGCGCGTCGCGACGCGGCGGCGCTCGCCGATGCGCATGAAT
GGGACCGGCGCGCGCGCCACGGCGGCGGCGCGCCGCTTGCCGGTGCGGTGCTGACGGTGAAGGCGTGTTTCGACTGCGAA
GGCTGGGTCACGCACGCAGGCTCGCGCGTACTCGCCGACGATCCGCCGGCACGCACCGACGCGGCGCTCGTCGCGGCGCT
GCGACGCGTCGGCGCCGTGCTCGTCGCGCAGACCGCGATGACGGAGTTCGCGTACGGCGCGCTCGGTGTGAACCGCACCT
ACGGCACGCCGGCAACGCCGCTCGACGCGCATCGCGAGCGCGTGGCAGGCGGGTCGAGTTCGGGCGCCGCGGTGTCGATC
GCGCTCGGCGTCGCCGATCTGTCGGTCGGCTCGGACACGAGCGGATCGGCCCGCATTCCGGCCGCATTCTGCGGCGTGGC
AGGGTTCAAGCCGTCGCGCGGCCGCTATCCCGATGCGGGCATGCAATTCCTGTCGACGACGTTCGACGTGCCGGGCCTGA
TCGCCGCGACGGCCGCGCGATGCCGCGACGCGGACGCCGCGCTGCACGGCCGTGCGGCTGCTGCCGCGACACCGGCGCGT
GCGCGCATCGACGATCTGCATTTCGTCGTGCCCGAACGCTTCGCGACCGACGACACCGATGCGCACGTCGCGCAGGCGTT
CGACGCGTGGCTGTCGCGTCTATCGGCGTGCGGTGCGCGCATCGAGCGCAAGCGGCTCGATTGCATCGTCGAGGCTGGCG
CGGTTGCGCGCGAGGGCGGCATCATCGCGGCCGAGGCGTTCAGTCTGCATCGCGCGCGACTCGCGACATGCGCGGATCGC
TACGACCCGCTCGTCGCCGCACGTATTGCAGCGGGCGAGCAGGTGCGCGCACACGACTATGCGGCCGCGCTCGCGCGTCT
GCGCGCGCTGGCCGCTTCGTACGACGCGGCGCTCGGCGAAGCGGACGCGGTGCTCACGCCGACGGTGCCGATGCCGCCGC
CGCCTATCGACGAGCTGGCCGACACCGCCGTGTATCTCGCGCAGAATGCGCGCGCCTTCCGGCTCACCGAGTTCGCGAAC
CGGCTCGATCTGCCGAGCATCAGCGTGCCGGCGGAGCGCCGGCGGAACCGGCCGATCGGGCTGCTGATGACCGGACGCCG
CGGCCGCGATACAAGCCTGCTCGATGCGGCCGTGCGCGTCGAACACGCGCTGGACGCGTGCAGATGA

Upstream 100 bases:

>100_bases
TACGACGTGCCGCCCGAGCGCATACGGGCGAGCGGCGACGCGCGTGTGAAGGATTTTCTGCGGCATGTTCACTTCGCATG
ATCGGGCACGAGGCTCACGC

Downstream 100 bases:

>100_bases
ACCGTTTTCCGTCGGCGGGGCACGCCCTGCCGTTTTTCATCGTCACCATTCCGAGGTGCTCATGATCGTCGTAAGATCGG
CCGACATTGCCCACACCGAA

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNGFDFTYDERRDARPCIDRLRACIARAAAHPDDISTAWLARRDAAALADAHEWDRRARHGGGAPLAGAVLTVKACFDCE
GWVTHAGSRVLADDPPARTDAALVAALRRVGAVLVAQTAMTEFAYGALGVNRTYGTPATPLDAHRERVAGGSSSGAAVSI
ALGVADLSVGSDTSGSARIPAAFCGVAGFKPSRGRYPDAGMQFLSTTFDVPGLIAATAARCRDADAALHGRAAAAATPAR
ARIDDLHFVVPERFATDDTDAHVAQAFDAWLSRLSACGARIERKRLDCIVEAGAVAREGGIIAAEAFSLHRARLATCADR
YDPLVAARIAAGEQVRAHDYAAALARLRALAASYDAALGEADAVLTPTVPMPPPPIDELADTAVYLAQNARAFRLTEFAN
RLDLPSISVPAERRRNRPIGLLMTGRRGRDTSLLDAAVRVEHALDACR

Sequences:

>Translated_448_residues
MNGFDFTYDERRDARPCIDRLRACIARAAAHPDDISTAWLARRDAAALADAHEWDRRARHGGGAPLAGAVLTVKACFDCE
GWVTHAGSRVLADDPPARTDAALVAALRRVGAVLVAQTAMTEFAYGALGVNRTYGTPATPLDAHRERVAGGSSSGAAVSI
ALGVADLSVGSDTSGSARIPAAFCGVAGFKPSRGRYPDAGMQFLSTTFDVPGLIAATAARCRDADAALHGRAAAAATPAR
ARIDDLHFVVPERFATDDTDAHVAQAFDAWLSRLSACGARIERKRLDCIVEAGAVAREGGIIAAEAFSLHRARLATCADR
YDPLVAARIAAGEQVRAHDYAAALARLRALAASYDAALGEADAVLTPTVPMPPPPIDELADTAVYLAQNARAFRLTEFAN
RLDLPSISVPAERRRNRPIGLLMTGRRGRDTSLLDAAVRVEHALDACR
>Mature_448_residues
MNGFDFTYDERRDARPCIDRLRACIARAAAHPDDISTAWLARRDAAALADAHEWDRRARHGGGAPLAGAVLTVKACFDCE
GWVTHAGSRVLADDPPARTDAALVAALRRVGAVLVAQTAMTEFAYGALGVNRTYGTPATPLDAHRERVAGGSSSGAAVSI
ALGVADLSVGSDTSGSARIPAAFCGVAGFKPSRGRYPDAGMQFLSTTFDVPGLIAATAARCRDADAALHGRAAAAATPAR
ARIDDLHFVVPERFATDDTDAHVAQAFDAWLSRLSACGARIERKRLDCIVEAGAVAREGGIIAAEAFSLHRARLATCADR
YDPLVAARIAAGEQVRAHDYAAALARLRALAASYDAALGEADAVLTPTVPMPPPPIDELADTAVYLAQNARAFRLTEFAN
RLDLPSISVPAERRRNRPIGLLMTGRRGRDTSLLDAAVRVEHALDACR

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=24.7950819672131, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI195972892, Length=255, Percent_Identity=30.1960784313725, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=411, Percent_Identity=27.4939172749392, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=197, Percent_Identity=31.4720812182741, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=192, Percent_Identity=29.6875, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=156, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=202, Percent_Identity=29.7029702970297, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=175, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=175, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=388, Percent_Identity=27.8350515463918, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=187, Percent_Identity=29.4117647058824, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=424, Percent_Identity=27.3584905660377, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648441, Length=408, Percent_Identity=23.2843137254902, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=408, Percent_Identity=23.2843137254902, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=447, Percent_Identity=23.0425055928412, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648437, Length=447, Percent_Identity=23.0425055928412, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550774, Length=408, Percent_Identity=23.2843137254902, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24644968, Length=204, Percent_Identity=30.8823529411765, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 47276; Mature: 47276

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFDFTYDERRDARPCIDRLRACIARAAAHPDDISTAWLARRDAAALADAHEWDRRARH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGGAPLAGAVLTVKACFDCEGWVTHAGSRVLADDPPARTDAALVAALRRVGAVLVAQTAM
CCCCCHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TEFAYGALGVNRTYGTPATPLDAHRERVAGGSSSGAAVSIALGVADLSVGSDTSGSARIP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCC
AAFCGVAGFKPSRGRYPDAGMQFLSTTFDVPGLIAATAARCRDADAALHGRAAAAATPAR
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHCCCCH
ARIDDLHFVVPERFATDDTDAHVAQAFDAWLSRLSACGARIERKRLDCIVEAGAVAREGG
HHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
IIAAEAFSLHRARLATCADRYDPLVAARIAAGEQVRAHDYAAALARLRALAASYDAALGE
EEEEHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ADAVLTPTVPMPPPPIDELADTAVYLAQNARAFRLTEFANRLDLPSISVPAERRRNRPIG
CCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCEE
LLMTGRRGRDTSLLDAAVRVEHALDACR
EEEECCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGFDFTYDERRDARPCIDRLRACIARAAAHPDDISTAWLARRDAAALADAHEWDRRARH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGGAPLAGAVLTVKACFDCEGWVTHAGSRVLADDPPARTDAALVAALRRVGAVLVAQTAM
CCCCCHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TEFAYGALGVNRTYGTPATPLDAHRERVAGGSSSGAAVSIALGVADLSVGSDTSGSARIP
HHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCC
AAFCGVAGFKPSRGRYPDAGMQFLSTTFDVPGLIAATAARCRDADAALHGRAAAAATPAR
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHCCCCH
ARIDDLHFVVPERFATDDTDAHVAQAFDAWLSRLSACGARIERKRLDCIVEAGAVAREGG
HHHHHHHEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
IIAAEAFSLHRARLATCADRYDPLVAARIAAGEQVRAHDYAAALARLRALAASYDAALGE
EEEEHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ADAVLTPTVPMPPPPIDELADTAVYLAQNARAFRLTEFANRLDLPSISVPAERRRNRPIG
CCCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCEE
LLMTGRRGRDTSLLDAAVRVEHALDACR
EEEECCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1465104 [H]