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The map label for this gene is citH [H]

Identifier: 189353167

GI number: 189353167

Start: 1385044

End: 1386357

Strand: Direct

Name: citH [H]

Synonym: BMULJ_04403

Alternate gene names: 189353167

Gene position: 1385044-1386357 (Clockwise)

Preceding gene: 189353166

Following gene: 189353176

Centisome position: 56.0

GC content: 68.26

Gene sequence:

>1314_bases
ATGGAAACGTCTACCCTTTCGTTCGCCGGCACGCCGGCCGATGCACTGACCGCCGCGAAAAAACGCCGCCTGATCGCGGC
GGCCGCCGTCGGCAACGCGCTCGAGTTCTACGACTTCACGGTCTACAGCTTCTTCGCGATCCTGATCGGCAAGCTGTTCT
TTCCCGTCCATTCGTCGTTCGGGCAGCTGATGCTCGCGGTCGCGAGTTTCGGCGTCGGTTTCGTCACGCGGCCGCTCGGC
GGGCTCGTGATCGGCATGTACGCCGATCGCGTCGGCCGCAAGAAAGCGATGATCCTGACGCTGCTGCTGATGGCGCTCGG
CACCGCGACGATCGCGGTCGCGCCGACCTTCGCGCAGATCGGCATCGCGGCGCCGCTGCTGCTCGTGCTTGCGCGTCTGC
TGCAAGGATTCGCGTCGGGCGGCGAAGTCGGCGCATCGACGACATTGCTGCTCGAACAGGCGCCGCAGCATCGTCGCGGC
TTCTACGCGTCGTTCCAGTTCTCGAGCCAGGGGCTCGCCGCGCTCGCGGGCGCGCTGACCGGCGTCGCGCTGACCTCGAC
GCTGAACGCCGCGCAGCTCGAAAGCTGGGGCTGGCGCGTGCCGTTCGTGATCGGCACGCTGTTCGTGCCGCTCGGCTACT
GGCTGCGCCGCACGGTCGAGGAAGTGCCGTCGGCCGCGCACGCGCCCGCCGAGAAGGTCGCGTCGCTGCCGCTCGCGGAC
GTGCTGCGCCATCACGGCAAGGCCGTGTTCGCGGGGCTCGGCGTGACGATCGGCGGCACGTCGATCCACTACATCATCGT
GTTCTACATGGCGATCTACGGCGTGCAGGTGCTGCATCTGCCGACCTGGCTGTCGATGACGGCCGGCTGCGTCGCGGGCG
CGATCCTGATGCTCGCGACGCCGATCGGCGGCTACCTGTCCGACGTGGTCGGCCGCAACCGGATCGTCTGGTGGACGCGC
GTTGCGCTGATGGTCGCGATCTACCCGGCCTTCATCGCGCTGAACCGCTGGCCGGGCGCCGTGTCGCTGCTGTCGATCAT
CGCCGCGCTGTCGGTCGTGCATGCGATCAACATCGGCGCGACCGGTGCGATGCTCGGCGAGCTGTTCCCGCGTGCGGTGC
GCGCGACGGGCGGCGCGCTCGTGTACAGCGTCGGCGTCGCGATCTTCGGCGGCTTCGCGCAGTTCTTCGTCACCTGGCTG
ATCGCGGCAACCGGCAATCCGAACGCGCCGGCATGGTATGCGATCGGCTGCGGCGTGCTGACGCTGATCGCGGTGCGGTG
TATGGACGAGAAGGCTGGGAAGACGCTTGATTGA

Upstream 100 bases:

>100_bases
TCCTGGCGATCGGCGCGAGCTACTGGGTGCGCGTCGCCGAAGGCTGGCTCGCGGCCTGACGGCCTGTCCTCACGCCGCTG
TGCGCTTATCGGGAAATCCC

Downstream 100 bases:

>100_bases
GCGGCGTCCGGTGCAAGGATGCGCGACGATGAATCGCCTGTCTCGCTATCGTTGCACACCACGACCATTCGCGAAACCGG
CCCCGCATGCGATGTCAGGC

Product: permease of the major facilitator superfamily

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
METSTLSFAGTPADALTAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSSFGQLMLAVASFGVGFVTRPLG
GLVIGMYADRVGRKKAMILTLLLMALGTATIAVAPTFAQIGIAAPLLLVLARLLQGFASGGEVGASTTLLLEQAPQHRRG
FYASFQFSSQGLAALAGALTGVALTSTLNAAQLESWGWRVPFVIGTLFVPLGYWLRRTVEEVPSAAHAPAEKVASLPLAD
VLRHHGKAVFAGLGVTIGGTSIHYIIVFYMAIYGVQVLHLPTWLSMTAGCVAGAILMLATPIGGYLSDVVGRNRIVWWTR
VALMVAIYPAFIALNRWPGAVSLLSIIAALSVVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNPNAPAWYAIGCGVLTLIAVRCMDEKAGKTLD

Sequences:

>Translated_437_residues
METSTLSFAGTPADALTAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSSFGQLMLAVASFGVGFVTRPLG
GLVIGMYADRVGRKKAMILTLLLMALGTATIAVAPTFAQIGIAAPLLLVLARLLQGFASGGEVGASTTLLLEQAPQHRRG
FYASFQFSSQGLAALAGALTGVALTSTLNAAQLESWGWRVPFVIGTLFVPLGYWLRRTVEEVPSAAHAPAEKVASLPLAD
VLRHHGKAVFAGLGVTIGGTSIHYIIVFYMAIYGVQVLHLPTWLSMTAGCVAGAILMLATPIGGYLSDVVGRNRIVWWTR
VALMVAIYPAFIALNRWPGAVSLLSIIAALSVVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNPNAPAWYAIGCGVLTLIAVRCMDEKAGKTLD
>Mature_437_residues
METSTLSFAGTPADALTAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSSFGQLMLAVASFGVGFVTRPLG
GLVIGMYADRVGRKKAMILTLLLMALGTATIAVAPTFAQIGIAAPLLLVLARLLQGFASGGEVGASTTLLLEQAPQHRRG
FYASFQFSSQGLAALAGALTGVALTSTLNAAQLESWGWRVPFVIGTLFVPLGYWLRRTVEEVPSAAHAPAEKVASLPLAD
VLRHHGKAVFAGLGVTIGGTSIHYIIVFYMAIYGVQVLHLPTWLSMTAGCVAGAILMLATPIGGYLSDVVGRNRIVWWTR
VALMVAIYPAFIALNRWPGAVSLLSIIAALSVVHAINIGATGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWL
IAATGNPNAPAWYAIGCGVLTLIAVRCMDEKAGKTLD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=436, Percent_Identity=30.045871559633, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1788942, Length=430, Percent_Identity=33.7209302325581, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788292, Length=440, Percent_Identity=30.6818181818182, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=31.0747663551402, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=28.3898305084746, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=21.3903743315508, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45906; Mature: 45906

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSTLSFAGTPADALTAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSSF
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQLMLAVASFGVGFVTRPLGGLVIGMYADRVGRKKAMILTLLLMALGTATIAVAPTFAQI
HHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEECHHHHHH
GIAAPLLLVLARLLQGFASGGEVGASTTLLLEQAPQHRRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHCCCEEEEEECCCCHHHHHHHHH
GVALTSTLNAAQLESWGWRVPFVIGTLFVPLGYWLRRTVEEVPSAAHAPAEKVASLPLAD
HHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHH
VLRHHGKAVFAGLGVTIGGTSIHYIIVFYMAIYGVQVLHLPTWLSMTAGCVAGAILMLAT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIGGYLSDVVGRNRIVWWTRVALMVAIYPAFIALNRWPGAVSLLSIIAALSVVHAINIGA
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNPNAPAWYAIGCGVL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLIAVRCMDEKAGKTLD
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure
METSTLSFAGTPADALTAAKKRRLIAAAAVGNALEFYDFTVYSFFAILIGKLFFPVHSSF
CCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQLMLAVASFGVGFVTRPLGGLVIGMYADRVGRKKAMILTLLLMALGTATIAVAPTFAQI
HHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEECHHHHHH
GIAAPLLLVLARLLQGFASGGEVGASTTLLLEQAPQHRRGFYASFQFSSQGLAALAGALT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHCCCEEEEEECCCCHHHHHHHHH
GVALTSTLNAAQLESWGWRVPFVIGTLFVPLGYWLRRTVEEVPSAAHAPAEKVASLPLAD
HHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHH
VLRHHGKAVFAGLGVTIGGTSIHYIIVFYMAIYGVQVLHLPTWLSMTAGCVAGAILMLAT
HHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIGGYLSDVVGRNRIVWWTRVALMVAIYPAFIALNRWPGAVSLLSIIAALSVVHAINIGA
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
TGAMLGELFPRAVRATGGALVYSVGVAIFGGFAQFFVTWLIAATGNPNAPAWYAIGCGVL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
TLIAVRCMDEKAGKTLD
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]