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The map label for this gene is hipO [H]

Identifier: 189353166

GI number: 189353166

Start: 1383818

End: 1385002

Strand: Direct

Name: hipO [H]

Synonym: BMULJ_04402

Alternate gene names: 189353166

Gene position: 1383818-1385002 (Clockwise)

Preceding gene: 189353163

Following gene: 189353167

Centisome position: 55.95

GC content: 69.7

Gene sequence:

>1185_bases
ATGCCCGCAGCCGTGAACCCCGTCATCCCCGGCATCGCCGCGATCGCGCCCGAACTCATCGACGTGCGGCGCCGCATCCA
CGCCCATCCCGAACTCGCCTTCGAAGAAACGCTGACGAGCGATCTCGTCGCCGAGCTGCTCGCCGGCTGGGGCTACGACG
TGCATCGCGGGATCGGCAAGACCGGCGTCGTCGGCGTGCTGCGCGAAGGCCAGGGTACGCGCACGGTCGGCTTGCGCGCC
GACATGGACGCGCTGCCGCTCGCCGAAGCGACCGGGCTGCCGTATGCGAGCCGCCACGCGAACAAGATGCATGCATGCGG
TCACGACGGCCATACCGCGATGCTGCTGTGCGCGGCGCGCCACCTCGCGGCCACGCGCCGCTTCTCGGGCACGCTGAACC
TGATCTTCCAGCCGGCCGAGGAAAACTTCGGCGGCGCGAAGGCGATGATGGACGACGGGCTGTTCGACCGCTTCCCGTGC
GATGCGATCTTCGCGATCCACAACATGCCGGGCCGCGCGGCCGGCGACATGGCGTTCCGCAGCGGCGCGACGATGGCGTC
GGCCGACCGCGTGACGATCACGCTGCGCGGCGTCGGCGGTCACGGCGCGATGCCGCACTACGCGCGCGATCCGATGTCGG
CGGCCGGCAGCATCATGGTCGCGCTGCAGACGATCGTCGCGCGCGAAGTCGATGCACAGCACGCGGCGGTGATCACGGTC
GGCAGCGTGCAGGCCGGCGAGACCTTCAACGTGATTCCGGAAACGGTCGTGATGAAGCTGTCGGTGCGCGCGCTCGACGC
CGAGGTGCGCGCGCTGCTCGCGCGCCGCATCGAAGCGCTCGTGAAGGGTCAGGCCGAGAGCTTCGGCGTGACGGCCGACG
TCGACTACGACTACGGGTATCCGGTGCTCGTCAATCATGCGGAGCCCACGGCCTTCGCGGCCGACATCGCGCGCGGCATG
CTCGGCGACGATCGCGTGCAGCTCGACGCGCCGCCGCTGATGGGCAGCGAGGACTTCGCGTTCATGCTCGAAGCGCGGCC
CGGCTGCTATGCGTTCATCGGCAACGGAATCGGCAGCAAGGGCGGCTGCATGGTGCACAACCCCGGCTACGACTTCAACG
ACGACATCCTGGCGATCGGCGCGAGCTACTGGGTGCGCGTCGCCGAAGGCTGGCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
AGCACCGAAGCAATTTATCAGCTTCTGGTGCGCAAAAGAAATTTATATGCTTGCGGGTCATAAGCGCATCAAACGCCTCA
CCCGCCCCACCGGAGACACC

Downstream 100 bases:

>100_bases
CGGCCTGTCCTCACGCCGCTGTGCGCTTATCGGGAAATCCCATGGAAACGTCTACCCTTTCGTTCGCCGGCACGCCGGCC
GATGCACTGACCGCCGCGAA

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MPAAVNPVIPGIAAIAPELIDVRRRIHAHPELAFEETLTSDLVAELLAGWGYDVHRGIGKTGVVGVLREGQGTRTVGLRA
DMDALPLAEATGLPYASRHANKMHACGHDGHTAMLLCAARHLAATRRFSGTLNLIFQPAEENFGGAKAMMDDGLFDRFPC
DAIFAIHNMPGRAAGDMAFRSGATMASADRVTITLRGVGGHGAMPHYARDPMSAAGSIMVALQTIVAREVDAQHAAVITV
GSVQAGETFNVIPETVVMKLSVRALDAEVRALLARRIEALVKGQAESFGVTADVDYDYGYPVLVNHAEPTAFAADIARGM
LGDDRVQLDAPPLMGSEDFAFMLEARPGCYAFIGNGIGSKGGCMVHNPGYDFNDDILAIGASYWVRVAEGWLAA

Sequences:

>Translated_394_residues
MPAAVNPVIPGIAAIAPELIDVRRRIHAHPELAFEETLTSDLVAELLAGWGYDVHRGIGKTGVVGVLREGQGTRTVGLRA
DMDALPLAEATGLPYASRHANKMHACGHDGHTAMLLCAARHLAATRRFSGTLNLIFQPAEENFGGAKAMMDDGLFDRFPC
DAIFAIHNMPGRAAGDMAFRSGATMASADRVTITLRGVGGHGAMPHYARDPMSAAGSIMVALQTIVAREVDAQHAAVITV
GSVQAGETFNVIPETVVMKLSVRALDAEVRALLARRIEALVKGQAESFGVTADVDYDYGYPVLVNHAEPTAFAADIARGM
LGDDRVQLDAPPLMGSEDFAFMLEARPGCYAFIGNGIGSKGGCMVHNPGYDFNDDILAIGASYWVRVAEGWLAA
>Mature_393_residues
PAAVNPVIPGIAAIAPELIDVRRRIHAHPELAFEETLTSDLVAELLAGWGYDVHRGIGKTGVVGVLREGQGTRTVGLRAD
MDALPLAEATGLPYASRHANKMHACGHDGHTAMLLCAARHLAATRRFSGTLNLIFQPAEENFGGAKAMMDDGLFDRFPCD
AIFAIHNMPGRAAGDMAFRSGATMASADRVTITLRGVGGHGAMPHYARDPMSAAGSIMVALQTIVAREVDAQHAAVITVG
SVQAGETFNVIPETVVMKLSVRALDAEVRALLARRIEALVKGQAESFGVTADVDYDYGYPVLVNHAEPTAFAADIARGML
GDDRVQLDAPPLMGSEDFAFMLEARPGCYAFIGNGIGSKGGCMVHNPGYDFNDDILAIGASYWVRVAEGWLAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=430, Percent_Identity=28.6046511627907, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41748; Mature: 41617

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAVNPVIPGIAAIAPELIDVRRRIHAHPELAFEETLTSDLVAELLAGWGYDVHRGIGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
TGVVGVLREGQGTRTVGLRADMDALPLAEATGLPYASRHANKMHACGHDGHTAMLLCAAR
CCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCCCHHHCCCHHHCCCCCCCHHHHHHHHH
HLAATRRFSGTLNLIFQPAEENFGGAKAMMDDGLFDRFPCDAIFAIHNMPGRAAGDMAFR
HHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHH
SGATMASADRVTITLRGVGGHGAMPHYARDPMSAAGSIMVALQTIVAREVDAQHAAVITV
CCCCEECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GSVQAGETFNVIPETVVMKLSVRALDAEVRALLARRIEALVKGQAESFGVTADVDYDYGY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCC
PVLVNHAEPTAFAADIARGMLGDDRVQLDAPPLMGSEDFAFMLEARPGCYAFIGNGIGSK
CEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCC
GGCMVHNPGYDFNDDILAIGASYWVRVAEGWLAA
CCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAAVNPVIPGIAAIAPELIDVRRRIHAHPELAFEETLTSDLVAELLAGWGYDVHRGIGK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
TGVVGVLREGQGTRTVGLRADMDALPLAEATGLPYASRHANKMHACGHDGHTAMLLCAAR
CCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCCCHHHCCCHHHCCCCCCCHHHHHHHHH
HLAATRRFSGTLNLIFQPAEENFGGAKAMMDDGLFDRFPCDAIFAIHNMPGRAAGDMAFR
HHHHHHHCCCEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHH
SGATMASADRVTITLRGVGGHGAMPHYARDPMSAAGSIMVALQTIVAREVDAQHAAVITV
CCCCEECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
GSVQAGETFNVIPETVVMKLSVRALDAEVRALLARRIEALVKGQAESFGVTADVDYDYGY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCC
PVLVNHAEPTAFAADIARGMLGDDRVQLDAPPLMGSEDFAFMLEARPGCYAFIGNGIGSK
CEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCC
GGCMVHNPGYDFNDDILAIGASYWVRVAEGWLAA
CCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]