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The map label for this gene is narK [H]

Identifier: 189353123

GI number: 189353123

Start: 1314969

End: 1316294

Strand: Reverse

Name: narK [H]

Synonym: BMULJ_04359

Alternate gene names: 189353123

Gene position: 1316294-1314969 (Counterclockwise)

Preceding gene: 189353130

Following gene: 189353122

Centisome position: 53.22

GC content: 69.31

Gene sequence:

>1326_bases
ATGACCGACAAAGCTACCCGTATCGATCTCTTCAGCCTCCGCACCGCGCCGATGCGCGCGTTCCATCTGACGTGGATGGC
GTTCTTCGTCTGCTTCTTCGCGTGGTTCGCGTGCGCGCCGTTGATGCCGCTGATCGCACGCGAATTCCATCTGAGCGCCG
CACAGGTCGCGAACATCAACATCGCGGCCGTCGCCGCGACCATCGCGGTGCGGCTGCTCGTCGGCCCGCTGTGCGACCGC
TTCGGTCCGCGCCGCGTCTACGTCGGCCTGCTCGTGCTCGGCGCGATTCCGGTGTTCGCGGTGTCGTTCACGCACGACTA
CCTGTCGTTCCTGATCTGCCGGCTCGGGATCGGCGCGATCGGCGCGGGCTTCGTGATCACGCAGTACCACACGTCGGTGA
TGTTCGCGCCGAACGTCGTCGGCACCGCGAACGCGACGACGGCCGGCTGGGGCAACGCAGGCGCCGGCGCGACGCAGGCG
CTGATGCCGCTGCTCGTCGCGGCCGGCCTGATGCTCGGCCTCGGCGACGATTCGGCATGGCGCGCGGCGCTCGTCGTGCC
CGGCATCGCGATGCTCGTGATGGCGTGGGCGTACTGGCGCTATGCGCAGGACTGCCCGCAAGGCGACTTCATCGCGCTGC
GCAAAGCGGGCGTGACGATCGACAGCGGCAAGAAGGGCGGCTGGGCGAGCTTCTTCGCCGCGTGCGCCAACTATCGCGTG
TGGATGCTGTTCGTCACGTACGGCGCGTGCTTCGGCGTCGAGGTGTTCATCCACAACATCGCCGCGCTGTACTACGTCGA
TCACTTCCATCTGTCGCTGAAGGATGCGGGCCTCGCGGCCGGCCTGTTCGGGCTGCTCGCGCTGTTTGCGCGCGCACTCG
GCGGCTGGCTGTCGGACAAGGTCGCCGCGCGCCGCAGCCTCGACGTGCGGGCGACGCTGCTCTGTGCGCTGATCGTCGGC
GAAGGGCTCGGGCTGATCTGGTTCTCGCACGCCCAGAGCGTCGGCATCGCGCTCGTCGCGATGCTGACCTTCGGCCTGTT
CACGCACATGGCGTGCGGCGCGACCTATGCGCTCGTGCCGTTCATCGACCGCAAGGCGCTCGGCGGCGTCGCCGGGATCG
TCGGTGCGGGCGGCAACGCGGGCGCGGTCGCCGCGAGCTTCCTGCTGAAGGGCGTCGGCGACGTGCAGCACGCGTTGAGC
CTGCTCGGGCTCGCCGTTACCGCGACGGCGCTGTGCGCGATGGCCGTGCGCTTCAGCGAAGAACACAAGGCGCGCGAGGC
CGAGCTGCGCGACCGCGCACTCGCTGCCGCGAACGCCGCGAACTGA

Upstream 100 bases:

>100_bases
GCGCCCGGCAACGGGCTTCATGGGCAACGGCGTCCTACGCACCGGCTCTCGACAGACGAGACCGTGTGCGCAGGACGCCG
TTTTTCGTTTGGCGGATGAC

Downstream 100 bases:

>100_bases
TCGACCGAAGGAAACGCCATCATGAAACTCATCGTCATCGGCCACGGGATGGTCGGCCACAAGCTCCTCGAATGCGTCGC
CGCCGAAACGGCGGCGGTGC

Product: nitrate transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTDKATRIDLFSLRTAPMRAFHLTWMAFFVCFFAWFACAPLMPLIAREFHLSAAQVANINIAAVAATIAVRLLVGPLCDR
FGPRRVYVGLLVLGAIPVFAVSFTHDYLSFLICRLGIGAIGAGFVITQYHTSVMFAPNVVGTANATTAGWGNAGAGATQA
LMPLLVAAGLMLGLGDDSAWRAALVVPGIAMLVMAWAYWRYAQDCPQGDFIALRKAGVTIDSGKKGGWASFFAACANYRV
WMLFVTYGACFGVEVFIHNIAALYYVDHFHLSLKDAGLAAGLFGLLALFARALGGWLSDKVAARRSLDVRATLLCALIVG
EGLGLIWFSHAQSVGIALVAMLTFGLFTHMACGATYALVPFIDRKALGGVAGIVGAGGNAGAVAASFLLKGVGDVQHALS
LLGLAVTATALCAMAVRFSEEHKAREAELRDRALAAANAAN

Sequences:

>Translated_441_residues
MTDKATRIDLFSLRTAPMRAFHLTWMAFFVCFFAWFACAPLMPLIAREFHLSAAQVANINIAAVAATIAVRLLVGPLCDR
FGPRRVYVGLLVLGAIPVFAVSFTHDYLSFLICRLGIGAIGAGFVITQYHTSVMFAPNVVGTANATTAGWGNAGAGATQA
LMPLLVAAGLMLGLGDDSAWRAALVVPGIAMLVMAWAYWRYAQDCPQGDFIALRKAGVTIDSGKKGGWASFFAACANYRV
WMLFVTYGACFGVEVFIHNIAALYYVDHFHLSLKDAGLAAGLFGLLALFARALGGWLSDKVAARRSLDVRATLLCALIVG
EGLGLIWFSHAQSVGIALVAMLTFGLFTHMACGATYALVPFIDRKALGGVAGIVGAGGNAGAVAASFLLKGVGDVQHALS
LLGLAVTATALCAMAVRFSEEHKAREAELRDRALAAANAAN
>Mature_440_residues
TDKATRIDLFSLRTAPMRAFHLTWMAFFVCFFAWFACAPLMPLIAREFHLSAAQVANINIAAVAATIAVRLLVGPLCDRF
GPRRVYVGLLVLGAIPVFAVSFTHDYLSFLICRLGIGAIGAGFVITQYHTSVMFAPNVVGTANATTAGWGNAGAGATQAL
MPLLVAAGLMLGLGDDSAWRAALVVPGIAMLVMAWAYWRYAQDCPQGDFIALRKAGVTIDSGKKGGWASFFAACANYRVW
MLFVTYGACFGVEVFIHNIAALYYVDHFHLSLKDAGLAAGLFGLLALFARALGGWLSDKVAARRSLDVRATLLCALIVGE
GLGLIWFSHAQSVGIALVAMLTFGLFTHMACGATYALVPFIDRKALGGVAGIVGAGGNAGAVAASFLLKGVGDVQHALSL
LGLAVTATALCAMAVRFSEEHKAREAELRDRALAAANAAN

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46504; Mature: 46373

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKATRIDLFSLRTAPMRAFHLTWMAFFVCFFAWFACAPLMPLIAREFHLSAAQVANIN
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCH
IAAVAATIAVRLLVGPLCDRFGPRRVYVGLLVLGAIPVFAVSFTHDYLSFLICRLGIGAI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GAGFVITQYHTSVMFAPNVVGTANATTAGWGNAGAGATQALMPLLVAAGLMLGLGDDSAW
CCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHH
RAALVVPGIAMLVMAWAYWRYAQDCPQGDFIALRKAGVTIDSGKKGGWASFFAACANYRV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHCCHH
WMLFVTYGACFGVEVFIHNIAALYYVDHFHLSLKDAGLAAGLFGLLALFARALGGWLSDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
VAARRSLDVRATLLCALIVGEGLGLIWFSHAQSVGIALVAMLTFGLFTHMACGATYALVP
HHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FIDRKALGGVAGIVGAGGNAGAVAASFLLKGVGDVQHALSLLGLAVTATALCAMAVRFSE
HHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHKAREAELRDRALAAANAAN
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDKATRIDLFSLRTAPMRAFHLTWMAFFVCFFAWFACAPLMPLIAREFHLSAAQVANIN
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCH
IAAVAATIAVRLLVGPLCDRFGPRRVYVGLLVLGAIPVFAVSFTHDYLSFLICRLGIGAI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GAGFVITQYHTSVMFAPNVVGTANATTAGWGNAGAGATQALMPLLVAAGLMLGLGDDSAW
CCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHH
RAALVVPGIAMLVMAWAYWRYAQDCPQGDFIALRKAGVTIDSGKKGGWASFFAACANYRV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHCCHH
WMLFVTYGACFGVEVFIHNIAALYYVDHFHLSLKDAGLAAGLFGLLALFARALGGWLSDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
VAARRSLDVRATLLCALIVGEGLGLIWFSHAQSVGIALVAMLTFGLFTHMACGATYALVP
HHHHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FIDRKALGGVAGIVGAGGNAGAVAASFLLKGVGDVQHALSLLGLAVTATALCAMAVRFSE
HHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHKAREAELRDRALAAANAAN
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]