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The map label for this gene is pckA [H]

Identifier: 189353090

GI number: 189353090

Start: 1271554

End: 1273419

Strand: Reverse

Name: pckA [H]

Synonym: BMULJ_04326

Alternate gene names: 189353090

Gene position: 1273419-1271554 (Counterclockwise)

Preceding gene: 189353095

Following gene: 189353088

Centisome position: 51.49

GC content: 66.4

Gene sequence:

>1866_bases
ATGTCGCACAACAATCTGGCCGAAGCGACACACGGCCCGCTGATCGCTCCCCCCGCTTTCGTCAAACACCGCAAACTGAT
CGACTGGGTCGCGCGCATCGCCGAACTGACGGAGCCCGAACGCGTCGTCTGGTGCGACGGCTCGCAGGCCGAATACGACG
CGCTGTGTCAGGCGATGGTCGATCAGGGCACGCTCAAGCGCCTGAATCCGGCGAAGCGCCCGAACTCGTATCTCGCGCAA
TCCGATCCGTCGGACGTCGCGCGCGTCGAAGACCGCACGTTCATCTGCGCCGCGTCGCGCGACGACGTCGGCCCGACCAA
CAACTGGATCGCGCCCGACGAAATGCGCGAAACGCTCGACGGGCTGTTCCGCGGCTCGATGCGCGGCCGCACGCTCTATG
TCGTGCCGTTCTCGATGGGCCCGCTCGGCTCGCCGATCGCGCATATCGGCGTCGAGCTGTCCGATAGCCCGTACGTCGCG
GTGAACATGCGGATCATGACGCGCATGGGACGCGACGTGTACGACGTGCTCGGCGAAGACGGCGAATTCGTGCCGTGCGT
GCACAGCGTCGGTCATCCCCTCGAGCCCGGTCAGCAGGACGTGCCGTGGCCGTGCAATCCGGTCAAGTACATCGTGCATT
TCCCCGAGACGCGCGAGATCTGGAGCTTCGGCTCGGGCTACGGCGGCAATGCGCTGCTCGGCAAGAAGTGCTTTGCGCTG
CGGATCGCATCGACGATGGGCCGCGATCAGGGCTGGCTCGCCGAGCACATGCTGATCCTCGGTGTGACGTCGCCGGCCGG
CAAGAAGTATCACGTCGCGGCCGCGTTCCCGTCCGCATGCGGCAAGACGAACTTCGCGATGCTGATTCCGCCGCAGGGCT
TCGACGGCTGGAAGGTCACGACGATCGGCGACGATATCGCGTGGCTGAAGCCGGGCGCCGACGGCCGGCTCTACGCGATC
AACCCGGAGGCCGGCTATTTCGGCGTCGCGCCCGGCACCGGCGAGAAGACGAATCCGAATGCGCTCGCGACGCTGAAGGA
AAACGTGATCTTCACGAACGTCGCGCTGACCGAAGACGGCGACGTCTGGTGGGAAGGGCTGACCGACACGCCGCCGGCGA
AGCTGACCGACTGGCAAGGCAATACGTGGACGCCGGAAATCGGCAAGGAAACCGGGCGCAAGGCCGCGCATCCGAACTCG
CGCTTCACCGCGCCGGCCGCGCAGTGCCCGTCGATCGACGCCGACTGGGAGAACCCGGCCGGCGTACCGATCGATGCGTT
CATCTTCGGCGGACGCCGCTCGACCACGGTGCCGCTCGTGACCGAAGCGCGCGACTGGGTCGAAGGCGTATACATGGCCG
CGACGATGGGTTCCGAAACGACGGCCGCCGCCGCCGGCCAGCAGGGCGTCGTGCGGCGCGACCCGTTCGCGATGCTGCCG
TTCTGCGGCTACAACATGAGCGACTACTTCGCGCACTGGCTGAAGCTCGGCAAGCAGCTGGAGCGCGCCGGCGCGACGCT
GCCGAAGATCTATTGCGTGAACTGGTTCCGCAAGGACGCGGACGGCCGCTTCGTCTGGCCGGGTTTCGGCGAGAACATGC
GCGTGCTGAAGTGGATGCTCGATCGTCTCGAAGGCGAGGGCGGCGGCAGCGAGCATGCGTTCGGCGTGTCGCCCGCGTAT
GACGATCTGCACTGGGACGGCCTCGACTTCACGCGCGAGCAGTTCGACGCGGTCACGTCGATGAACGCGGAAGAATGGCG
CGAAGAGCTGAAGCTGCACGCGGCGCTGTTCGATACGCTGAAGCTGCGGCTGCCCGCTGAACTGACCGACACGATGAAGA
AGATCGAGCAGCGTCTCGACGCCTGA

Upstream 100 bases:

>100_bases
GACCTGCGCGTCGCCGCATCCTGCCCTCCGCAAGCATTCCGCATCTCGCGGGCCTCGCCCGCATCCCCGATCCCCGCCCA
ATAAATCTGGAGACATCATC

Downstream 100 bases:

>100_bases
CGATCCACATCGTCCGCGACGCCCCGCTGCCGGCACGGCGCCGGCAGCGGGGCGTTTTTTTCAGCGACACGAAAGGCAAC
GCGCTGCGGCAGCGGATGCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MSHNNLAEATHGPLIAPPAFVKHRKLIDWVARIAELTEPERVVWCDGSQAEYDALCQAMVDQGTLKRLNPAKRPNSYLAQ
SDPSDVARVEDRTFICAASRDDVGPTNNWIAPDEMRETLDGLFRGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVA
VNMRIMTRMGRDVYDVLGEDGEFVPCVHSVGHPLEPGQQDVPWPCNPVKYIVHFPETREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDQGWLAEHMLILGVTSPAGKKYHVAAAFPSACGKTNFAMLIPPQGFDGWKVTTIGDDIAWLKPGADGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLKENVIFTNVALTEDGDVWWEGLTDTPPAKLTDWQGNTWTPEIGKETGRKAAHPNS
RFTAPAAQCPSIDADWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLP
FCGYNMSDYFAHWLKLGKQLERAGATLPKIYCVNWFRKDADGRFVWPGFGENMRVLKWMLDRLEGEGGGSEHAFGVSPAY
DDLHWDGLDFTREQFDAVTSMNAEEWREELKLHAALFDTLKLRLPAELTDTMKKIEQRLDA

Sequences:

>Translated_621_residues
MSHNNLAEATHGPLIAPPAFVKHRKLIDWVARIAELTEPERVVWCDGSQAEYDALCQAMVDQGTLKRLNPAKRPNSYLAQ
SDPSDVARVEDRTFICAASRDDVGPTNNWIAPDEMRETLDGLFRGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVA
VNMRIMTRMGRDVYDVLGEDGEFVPCVHSVGHPLEPGQQDVPWPCNPVKYIVHFPETREIWSFGSGYGGNALLGKKCFAL
RIASTMGRDQGWLAEHMLILGVTSPAGKKYHVAAAFPSACGKTNFAMLIPPQGFDGWKVTTIGDDIAWLKPGADGRLYAI
NPEAGYFGVAPGTGEKTNPNALATLKENVIFTNVALTEDGDVWWEGLTDTPPAKLTDWQGNTWTPEIGKETGRKAAHPNS
RFTAPAAQCPSIDADWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLP
FCGYNMSDYFAHWLKLGKQLERAGATLPKIYCVNWFRKDADGRFVWPGFGENMRVLKWMLDRLEGEGGGSEHAFGVSPAY
DDLHWDGLDFTREQFDAVTSMNAEEWREELKLHAALFDTLKLRLPAELTDTMKKIEQRLDA
>Mature_620_residues
SHNNLAEATHGPLIAPPAFVKHRKLIDWVARIAELTEPERVVWCDGSQAEYDALCQAMVDQGTLKRLNPAKRPNSYLAQS
DPSDVARVEDRTFICAASRDDVGPTNNWIAPDEMRETLDGLFRGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAV
NMRIMTRMGRDVYDVLGEDGEFVPCVHSVGHPLEPGQQDVPWPCNPVKYIVHFPETREIWSFGSGYGGNALLGKKCFALR
IASTMGRDQGWLAEHMLILGVTSPAGKKYHVAAAFPSACGKTNFAMLIPPQGFDGWKVTTIGDDIAWLKPGADGRLYAIN
PEAGYFGVAPGTGEKTNPNALATLKENVIFTNVALTEDGDVWWEGLTDTPPAKLTDWQGNTWTPEIGKETGRKAAHPNSR
FTAPAAQCPSIDADWENPAGVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPF
CGYNMSDYFAHWLKLGKQLERAGATLPKIYCVNWFRKDADGRFVWPGFGENMRVLKWMLDRLEGEGGGSEHAFGVSPAYD
DLHWDGLDFTREQFDAVTSMNAEEWREELKLHAALFDTLKLRLPAELTDTMKKIEQRLDA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=632, Percent_Identity=49.2088607594937, Blast_Score=591, Evalue=1e-169,
Organism=Homo sapiens, GI66346721, Length=606, Percent_Identity=51.3201320132013, Blast_Score=587, Evalue=1e-167,
Organism=Homo sapiens, GI66346723, Length=387, Percent_Identity=54.5219638242894, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71989645, Length=626, Percent_Identity=48.8817891373802, Blast_Score=572, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI71989658, Length=626, Percent_Identity=48.8817891373802, Blast_Score=570, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17555492, Length=624, Percent_Identity=50.6410256410256, Blast_Score=570, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989653, Length=598, Percent_Identity=50, Blast_Score=561, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI32564411, Length=566, Percent_Identity=53.1802120141343, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564407, Length=551, Percent_Identity=51.9056261343013, Blast_Score=518, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=501, Percent_Identity=52.6946107784431, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32564409, Length=377, Percent_Identity=50.3978779840849, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17540956, Length=523, Percent_Identity=26.1950286806883, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24655085, Length=598, Percent_Identity=50.3344481605351, Blast_Score=586, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24655082, Length=607, Percent_Identity=48.4349258649094, Blast_Score=566, Evalue=1e-161,
Organism=Drosophila melanogaster, GI161077226, Length=576, Percent_Identity=48.9583333333333, Blast_Score=541, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24655088, Length=510, Percent_Identity=52.156862745098, Blast_Score=522, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 68485; Mature: 68354

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHNNLAEATHGPLIAPPAFVKHRKLIDWVARIAELTEPERVVWCDGSQAEYDALCQAMV
CCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
DQGTLKRLNPAKRPNSYLAQSDPSDVARVEDRTFICAASRDDVGPTNNWIAPDEMRETLD
CCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
GLFRGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRDVYDVLGED
HHHHCCCCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHCCHHHHHHCCC
GEFVPCVHSVGHPLEPGQQDVPWPCNPVKYIVHFPETREIWSFGSGYGGNALLGKKCFAL
CCEEHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHH
RIASTMGRDQGWLAEHMLILGVTSPAGKKYHVAAAFPSACGKTNFAMLIPPQGFDGWKVT
HHHHHCCCCCCHHHHEEEEEEECCCCCCEEEEEEECCHHCCCCCEEEEECCCCCCCEEEE
TIGDDIAWLKPGADGRLYAINPEAGYFGVAPGTGEKTNPNALATLKENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCHHEEECCCEEEEEEEEECCC
DVWWEGLTDTPPAKLTDWQGNTWTPEIGKETGRKAAHPNSRFTAPAAQCPSIDADWENPA
CEEECCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLP
CCCEEEEEECCCCCCCCEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECC
FCGYNMSDYFAHWLKLGKQLERAGATLPKIYCVNWFRKDADGRFVWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRLEGEGGGSEHAFGVSPAYDDLHWDGLDFTREQFDAVTSMNAEEWREELKLHAALFDTL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLRLPAELTDTMKKIEQRLDA
HEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHNNLAEATHGPLIAPPAFVKHRKLIDWVARIAELTEPERVVWCDGSQAEYDALCQAMV
CCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
DQGTLKRLNPAKRPNSYLAQSDPSDVARVEDRTFICAASRDDVGPTNNWIAPDEMRETLD
CCCHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
GLFRGSMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGRDVYDVLGED
HHHHCCCCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEHHHHHHCCHHHHHHCCC
GEFVPCVHSVGHPLEPGQQDVPWPCNPVKYIVHFPETREIWSFGSGYGGNALLGKKCFAL
CCEEHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHCCCCCCCCCCHHCCHHHHH
RIASTMGRDQGWLAEHMLILGVTSPAGKKYHVAAAFPSACGKTNFAMLIPPQGFDGWKVT
HHHHHCCCCCCHHHHEEEEEEECCCCCCEEEEEEECCHHCCCCCEEEEECCCCCCCEEEE
TIGDDIAWLKPGADGRLYAINPEAGYFGVAPGTGEKTNPNALATLKENVIFTNVALTEDG
EECCCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCHHEEECCCEEEEEEEEECCC
DVWWEGLTDTPPAKLTDWQGNTWTPEIGKETGRKAAHPNSRFTAPAAQCPSIDADWENPA
CEEECCCCCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
GVPIDAFIFGGRRSTTVPLVTEARDWVEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLP
CCCEEEEEECCCCCCCCEEEHHHHHHHHHEEEEEECCCCHHHHHCCCCCCEECCCCEECC
FCGYNMSDYFAHWLKLGKQLERAGATLPKIYCVNWFRKDADGRFVWPGFGENMRVLKWML
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCEECCCCCCCHHHHHHHH
DRLEGEGGGSEHAFGVSPAYDDLHWDGLDFTREQFDAVTSMNAEEWREELKLHAALFDTL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLRLPAELTDTMKKIEQRLDA
HEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA