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The map label for this gene is xylF [H]

Identifier: 189353062

GI number: 189353062

Start: 1232701

End: 1233729

Strand: Reverse

Name: xylF [H]

Synonym: BMULJ_04296

Alternate gene names: 189353062

Gene position: 1233729-1232701 (Counterclockwise)

Preceding gene: 189353063

Following gene: 189353061

Centisome position: 49.88

GC content: 65.99

Gene sequence:

>1029_bases
ATGAGATCCGTGACGCGTCGTACCGTATTGAGTTCGCTCGCCGGCGCCGCAGCGCTGGCGGTCCTCGCGCTTGGCGCGCC
GCTCGCGCACGCGAGCAAGGACAAGCCCGAGATCGGCTTCTGCATCGACGATCTGCGCGTCGAGCGCTGGTCGCGCGATC
GCGACTACTTCGTCGCCGCCGCGACGAAGCTCGGCGCGAAGGTGTCCGTGCAGTCCGCCGACGCGAGCGAGGAGCGGCAG
ATTTCGCAGATCGAGAACCTGATTTCGCGCGGCGTCGACGTGATCGTGATCGTGCCGTTCAACTCGAAGACGCTCGGCAA
CGTCGTCGCCGAAGCGAAGAAGGCCGGCATCAAGGTCGTGTCGTACGACCGATTGATTCTCGATGCGGACATCGACGCAT
ACATCTCGTTCGACAACGAGAAGGTCGGCGAGCTGCAGGCGCAGGGCGTGATCGACGCGAAGCCGAAGGGCAACTACTTC
CTGCTCGGCGGCGCACCGACCGACAACAACGCGAAGATGCTGCGCGCCGGCCAGATGAAGGTGCTGAAGCCCGCGATCGA
CCGCGGCGACATCAAGATCGTCGGCCAGCAGTGGGTGCCGGAGTGGAGCGCCTCGACCGCGCTGCGGATCGTCGAGGATG
CGCTGACCGCGAACAACAACAAGATCGACGCGATCGTCGCGTCGAACGACGGCACGGCCGGCGGCGCGATCCAGGCGCTC
GCCGCGCAGCATCTGGCCGGCAAGGTGCCCGTGTCGGGGCAGGACGCCGATCTCGCGGCCGTCAAGCGCGTGATCGCCGG
CACGCAGACGATGACCGTCTACAAGCCGCTGAAGCTGATCGCCGGCGAAGCGGCGAAGCTCGCGGTCGATCTCGCGAAGG
GCACGAAGCCCGCGTACAACGCGCAATACGACAACGGCAAGAAGAAGGTCGACACCGTGCTGCTGCAGCCGACGCTCCTG
ACCAAGCGCAACGTCGACGTCGTCGTGAAGGACGGCTTCTACACCCAGGCGCAGCTGGCGAGCCAGTAA

Upstream 100 bases:

>100_bases
CGCCGCGCGATGGCGCGGGGCCTGCAATGGCTCGAAGCGGGCGGCGCATTCCGGCGCCCGCCCGGCGTATCGAGGTAGTC
CAATAAAGAGGAGTGGAGAC

Downstream 100 bases:

>100_bases
GCGGCGCGACGCGCGCGGCCGGCCGCGGGTGGCGGGTCCGGCCGCGCGCACACTGCGAGGCACGAACGTATGACCGAACC
CTTGCTGACGATGCGCGGCA

Product: simple sugar transport system substrate-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRSVTRRTVLSSLAGAAALAVLALGAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEERQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAKKAGIKVVSYDRLILDADIDAYISFDNEKVGELQAQGVIDAKPKGNYF
LLGGAPTDNNAKMLRAGQMKVLKPAIDRGDIKIVGQQWVPEWSASTALRIVEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQHLAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGTKPAYNAQYDNGKKKVDTVLLQPTLL
TKRNVDVVVKDGFYTQAQLASQ

Sequences:

>Translated_342_residues
MRSVTRRTVLSSLAGAAALAVLALGAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEERQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAKKAGIKVVSYDRLILDADIDAYISFDNEKVGELQAQGVIDAKPKGNYF
LLGGAPTDNNAKMLRAGQMKVLKPAIDRGDIKIVGQQWVPEWSASTALRIVEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQHLAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGTKPAYNAQYDNGKKKVDTVLLQPTLL
TKRNVDVVVKDGFYTQAQLASQ
>Mature_342_residues
MRSVTRRTVLSSLAGAAALAVLALGAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAAATKLGAKVSVQSADASEERQ
ISQIENLISRGVDVIVIVPFNSKTLGNVVAEAKKAGIKVVSYDRLILDADIDAYISFDNEKVGELQAQGVIDAKPKGNYF
LLGGAPTDNNAKMLRAGQMKVLKPAIDRGDIKIVGQQWVPEWSASTALRIVEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQHLAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGTKPAYNAQYDNGKKKVDTVLLQPTLL
TKRNVDVVVKDGFYTQAQLASQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=312, Percent_Identity=63.1410256410256, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1790526, Length=247, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36347; Mature: 36347

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVTRRTVLSSLAGAAALAVLALGAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEE
ATKLGAKVSVQSADASEERQISQIENLISRGVDVIVIVPFNSKTLGNVVAEAKKAGIKVV
EHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEE
SYDRLILDADIDAYISFDNEKVGELQAQGVIDAKPKGNYFLLGGAPTDNNAKMLRAGQMK
EECEEEEECCCEEEEEECCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCCEEECCCCE
VLKPAIDRGDIKIVGQQWVPEWSASTALRIVEDALTANNNKIDAIVASNDGTAGGAIQAL
EEHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
AAQHLAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGTKPAYN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHEEEEHHCCCCCCCC
AQYDNGKKKVDTVLLQPTLLTKRNVDVVVKDGFYTQAQLASQ
CCCCCCCCEEEEEEECCCEECCCCCEEEEECCCCCHHHHCCC
>Mature Secondary Structure
MRSVTRRTVLSSLAGAAALAVLALGAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEHHHHHHHHHCCCCCEEEEE
ATKLGAKVSVQSADASEERQISQIENLISRGVDVIVIVPFNSKTLGNVVAEAKKAGIKVV
EHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEE
SYDRLILDADIDAYISFDNEKVGELQAQGVIDAKPKGNYFLLGGAPTDNNAKMLRAGQMK
EECEEEEECCCEEEEEECCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCCEEECCCCE
VLKPAIDRGDIKIVGQQWVPEWSASTALRIVEDALTANNNKIDAIVASNDGTAGGAIQAL
EEHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHH
AAQHLAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGTKPAYN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHHEEEEHHCCCCCCCC
AQYDNGKKKVDTVLLQPTLLTKRNVDVVVKDGFYTQAQLASQ
CCCCCCCCEEEEEEECCCEECCCCCEEEEECCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]