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The map label for this gene is nemR [H]

Identifier: 189353051

GI number: 189353051

Start: 1219016

End: 1219636

Strand: Direct

Name: nemR [H]

Synonym: BMULJ_04285

Alternate gene names: 189353051

Gene position: 1219016-1219636 (Clockwise)

Preceding gene: 189353050

Following gene: 189353052

Centisome position: 49.29

GC content: 66.18

Gene sequence:

>621_bases
ATGAATGCATCCTCGGGCGCGGAAGTCCGCCAGCACATCCTCAATATCGCGAAGCCGATCATGCTCCACAAGGGGTTTTC
GGCGGTCGGGCTGAACGAGATTCTCGCTGCGGCGGGCATCCCGAAGGGGTCGTTCTACCATTACTTCGGCTCGAAGGAGG
CGTTCGGCGAGGCGCTGCTCGAGTCGTATTTCGAAGGCTACCTCGAGCATCTCGACAACCTGTTCGAGCGCCAGCCCGGC
ACGGGCGCCGAGCGGATGATGACGTACTGGAGCAATTGGCTGCACACGCAGTGCGCGGACGATCCCGAGGGCAAGTGCCT
CGCGGTGAAGCTGGGCGCGGAAGTGTCCGACCTCTCCGAGGCGATGCGCGCGGTACTGCGGCGCGGCACGAGCCAGATCG
TCGAACGGCTGGCGGCATGCATCGAGGCCGGCCTCGCGGACGGTTCGCTGCGTGGCATCGAGGATCCGCCGCACACCGCC
GCGCTGCTGTACGAGCTGTGGCTCGGTGCGACGCTGCTGGAGAAGATCCATCGCAACCGCAAGCCGCTCGAAACCGCGAT
GGCCGCGACGCGGCAGCTGCTGAACCTGCCGCCGTCCGCGCCCGACGAGGTGCGGACATAA

Upstream 100 bases:

>100_bases
CCGCGAGCTGCAAACCCGCAAAGCGACCAAACTGCAACGCCCGCGACGTCGCCTCAACGCCGTTAGACGACCGGTCTAAT
CGCTGCTACCATACGCCATC

Downstream 100 bases:

>100_bases
GCGGGCGCGCTACGGCGCGCCTTGTTTTTACCTATCACACTAGACGACTGGTCTACTCATGACGATAGCGCTTCTTTCCC
CGTTTTCCGCCGCCGCAGCA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNASSGAEVRQHILNIAKPIMLHKGFSAVGLNEILAAAGIPKGSFYHYFGSKEAFGEALLESYFEGYLEHLDNLFERQPG
TGAERMMTYWSNWLHTQCADDPEGKCLAVKLGAEVSDLSEAMRAVLRRGTSQIVERLAACIEAGLADGSLRGIEDPPHTA
ALLYELWLGATLLEKIHRNRKPLETAMAATRQLLNLPPSAPDEVRT

Sequences:

>Translated_206_residues
MNASSGAEVRQHILNIAKPIMLHKGFSAVGLNEILAAAGIPKGSFYHYFGSKEAFGEALLESYFEGYLEHLDNLFERQPG
TGAERMMTYWSNWLHTQCADDPEGKCLAVKLGAEVSDLSEAMRAVLRRGTSQIVERLAACIEAGLADGSLRGIEDPPHTA
ALLYELWLGATLLEKIHRNRKPLETAMAATRQLLNLPPSAPDEVRT
>Mature_206_residues
MNASSGAEVRQHILNIAKPIMLHKGFSAVGLNEILAAAGIPKGSFYHYFGSKEAFGEALLESYFEGYLEHLDNLFERQPG
TGAERMMTYWSNWLHTQCADDPEGKCLAVKLGAEVSDLSEAMRAVLRRGTSQIVERLAACIEAGLADGSLRGIEDPPHTA
ALLYELWLGATLLEKIHRNRKPLETAMAATRQLLNLPPSAPDEVRT

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=197, Percent_Identity=39.0862944162437, Blast_Score=154, Evalue=4e-39,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22605; Mature: 22605

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASSGAEVRQHILNIAKPIMLHKGFSAVGLNEILAAAGIPKGSFYHYFGSKEAFGEALL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ESYFEGYLEHLDNLFERQPGTGAERMMTYWSNWLHTQCADDPEGKCLAVKLGAEVSDLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
AMRAVLRRGTSQIVERLAACIEAGLADGSLRGIEDPPHTAALLYELWLGATLLEKIHRNR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KPLETAMAATRQLLNLPPSAPDEVRT
CHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNASSGAEVRQHILNIAKPIMLHKGFSAVGLNEILAAAGIPKGSFYHYFGSKEAFGEALL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHH
ESYFEGYLEHLDNLFERQPGTGAERMMTYWSNWLHTQCADDPEGKCLAVKLGAEVSDLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHH
AMRAVLRRGTSQIVERLAACIEAGLADGSLRGIEDPPHTAALLYELWLGATLLEKIHRNR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
KPLETAMAATRQLLNLPPSAPDEVRT
CHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]