Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 189353045

GI number: 189353045

Start: 1211754

End: 1213061

Strand: Reverse

Name: shiA [H]

Synonym: BMULJ_04279

Alternate gene names: 189353045

Gene position: 1213061-1211754 (Counterclockwise)

Preceding gene: 189353047

Following gene: 189353043

Centisome position: 49.05

GC content: 67.74

Gene sequence:

>1308_bases
ATGACCCCGACCTTCGACACCCTCGACGCCGCTGCCGGCGCCCGGGCGCGCACCCAGGCCCGCAAGGCCGCGATCGGCAG
CTTCGTCGGCGCCGTCGTCGACTGGTACGACTTCCTGCTGTACGGGATTGTCGCCGCGCTCGTATTCAACGCCGAGTTCT
TTCCGAAAGTAAGCCCGACGATGGGGACGCTCGCGGCGTTCGCGACATTCGGCGTCGGCTTCCTGTTCCGTCCGCTCGGC
GGCGTCGTGTTCGGCCATTACGGCGACCGGCTCGGCCGCAAGCGAATGCTCGTGCTGACCGTGATGCTGATGGGGCTGTC
GACGGTCGCGATCGGGCTGCTGCCCACGTTCGCGACGATCGGCTGGTGGGCGCCCGTGCTGCTGGTCGCGATGCGCGCGA
TCCAGGGCTTCGCGGTCGGCGGCGAATGGGGCGGCGCGGCGCTGATGGCCGTCGAAAGTGCGCCGAAGCAGAAGAAGGCG
TTCTACAGCAGCGGCGTCCAGGTCGGCTACGGCGTCGGGCTGGTGCTCGCCACCGGCATCGTGTCGATTCTGAGCCACAC
GCTCGGCGACGCCGCGTTCAAAACCTGGGGCTGGCGCCTGCCGTTCGTGTTCAGCATCGTGCTGGTGCTGATCGGGCTGT
GGGTGCGCAAGAGCATGGACGAGTCGCAGGAGTTCGTCGAGAAGGTCGAGCACGGCCACCGCAAGCTGCGGCTGCCTGTG
CTGGAAGCGCTGACGCGTCATCCGAAGGCGTTTCTGCTGATCGTCGCGCTGCGGCTCGCGGAGCTGTTCACGATGTACAT
CGTCACCGCGTTCGCGCTCAGCTATTCGACGTCGAACCTCGGCATGTCGCGCGATCTGTTCCTGAACATCGGGCTGCTGG
TCGGCGCGGTGAGCTGCGTGACGATTCCGTGCTTCGCGTGGCTCGCCGATCGCTTCGGGCTGCGCCGCATCTATTTGATC
GGCGCGCTGATCGGCCTTGCATCGGCGGTGCCGTTCTTCGTCGCGCTCGAGTCGCGGTCGATCGCGTGGATCGTGCTGTT
CTCGATCCTGCTCGCGAACGCGGCGCACGACATGGTCGTCAGCGTCCAGCAGCCGCTGTTCACCGAACTGTTCGGCGCCG
AGTATCGATACAGCGGCGCGGGCGTCGGCTACCAGTTCGCGAGCGTCGTCGGCGGCGGGTTCACGCCGTTCATCGCGGTC
GGCCTCGTCAGCCTGGCCGGCGGCTCGTGGCACCTCGTCGCCGGTTATCTCGCGGCCGGCTGCCTGATTTCGCTCGTGGT
GGCGGCGCGGATGCGGGCTGCGGGCTGA

Upstream 100 bases:

>100_bases
TGCGGCGCGCGCCCTATTCTCCGTTCACTCATCGACCCAATGCAGCATCGGCGCGGCACCGGCCCGCGAGCCTGATGCGC
CCGTGCCAGGAGACATCAAC

Downstream 100 bases:

>100_bases
CGTCTTCCGCGCGGCTGCCAGGCGTGCGGCTCGATCACACGCGAACGAGACGCGGCACGCGAGTATAGAGGCGTTCGACA
AGCTCGCGCGCCTTTTCGCC

Product: shikimate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTPTFDTLDAAAGARARTQARKAAIGSFVGAVVDWYDFLLYGIVAALVFNAEFFPKVSPTMGTLAAFATFGVGFLFRPLG
GVVFGHYGDRLGRKRMLVLTVMLMGLSTVAIGLLPTFATIGWWAPVLLVAMRAIQGFAVGGEWGGAALMAVESAPKQKKA
FYSSGVQVGYGVGLVLATGIVSILSHTLGDAAFKTWGWRLPFVFSIVLVLIGLWVRKSMDESQEFVEKVEHGHRKLRLPV
LEALTRHPKAFLLIVALRLAELFTMYIVTAFALSYSTSNLGMSRDLFLNIGLLVGAVSCVTIPCFAWLADRFGLRRIYLI
GALIGLASAVPFFVALESRSIAWIVLFSILLANAAHDMVVSVQQPLFTELFGAEYRYSGAGVGYQFASVVGGGFTPFIAV
GLVSLAGGSWHLVAGYLAAGCLISLVVAARMRAAG

Sequences:

>Translated_435_residues
MTPTFDTLDAAAGARARTQARKAAIGSFVGAVVDWYDFLLYGIVAALVFNAEFFPKVSPTMGTLAAFATFGVGFLFRPLG
GVVFGHYGDRLGRKRMLVLTVMLMGLSTVAIGLLPTFATIGWWAPVLLVAMRAIQGFAVGGEWGGAALMAVESAPKQKKA
FYSSGVQVGYGVGLVLATGIVSILSHTLGDAAFKTWGWRLPFVFSIVLVLIGLWVRKSMDESQEFVEKVEHGHRKLRLPV
LEALTRHPKAFLLIVALRLAELFTMYIVTAFALSYSTSNLGMSRDLFLNIGLLVGAVSCVTIPCFAWLADRFGLRRIYLI
GALIGLASAVPFFVALESRSIAWIVLFSILLANAAHDMVVSVQQPLFTELFGAEYRYSGAGVGYQFASVVGGGFTPFIAV
GLVSLAGGSWHLVAGYLAAGCLISLVVAARMRAAG
>Mature_434_residues
TPTFDTLDAAAGARARTQARKAAIGSFVGAVVDWYDFLLYGIVAALVFNAEFFPKVSPTMGTLAAFATFGVGFLFRPLGG
VVFGHYGDRLGRKRMLVLTVMLMGLSTVAIGLLPTFATIGWWAPVLLVAMRAIQGFAVGGEWGGAALMAVESAPKQKKAF
YSSGVQVGYGVGLVLATGIVSILSHTLGDAAFKTWGWRLPFVFSIVLVLIGLWVRKSMDESQEFVEKVEHGHRKLRLPVL
EALTRHPKAFLLIVALRLAELFTMYIVTAFALSYSTSNLGMSRDLFLNIGLLVGAVSCVTIPCFAWLADRFGLRRIYLIG
ALIGLASAVPFFVALESRSIAWIVLFSILLANAAHDMVVSVQQPLFTELFGAEYRYSGAGVGYQFASVVGGGFTPFIAVG
LVSLAGGSWHLVAGYLAAGCLISLVVAARMRAAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=434, Percent_Identity=70.9677419354839, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=33.2547169811321, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=38.5844748858447, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788942, Length=406, Percent_Identity=32.2660098522167, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=25, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082231, Length=214, Percent_Identity=29.4392523364486, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46514; Mature: 46382

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPTFDTLDAAAGARARTQARKAAIGSFVGAVVDWYDFLLYGIVAALVFNAEFFPKVSPT
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCH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HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]