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The map label for this gene is mcrA [H]

Identifier: 189353044

GI number: 189353044

Start: 1210991

End: 1211719

Strand: Direct

Name: mcrA [H]

Synonym: BMULJ_04278

Alternate gene names: 189353044

Gene position: 1210991-1211719 (Clockwise)

Preceding gene: 189353042

Following gene: 189353046

Centisome position: 48.97

GC content: 61.73

Gene sequence:

>729_bases
ATGCCGACTTCTCCTACCCAAGCCCCTATATCCGACGAGGTTTCAGCACCTGCCGACGGACGACCATGGACGCATGAAGA
ACTTCGTGCCGCGGTCGACGCATATCTCGAAATGCTCGACAGCGAGCGCGCGAAGCGTCGCTATGTCAAGACAGAGGTCT
ATCGGGCGCTTGCCGAGCGATTCCATCGCACGGCAAAGGCATTCGAATACCGGATGCAGAATATTTCCTACGTGTTGGCG
CTGATGGGCCGCGATTGGCTACCCGGACTCAAACCGGCGAGAAACGTCGGCGCCAACGGCGTGCTGATCGAGCAGTTCAT
CAACGAGCGGGAATATCGAACGACGGCGCCCGTCGTCGAATTCGAGCTCGACGTTCGAGACCGGTTGAAGAGGGTGCCGG
TTGCGCCGCCGACAGGCAATGCTCGGCCGGAAGTCGGTTACGCGACCGTGGCGCAGGTTCGTCGCGATCCGGCCGTCAAG
GCGTGGGTATTGGCGCAGGCGCGCGGAACATGCGAATGCTGCGGCAATCCCGCGCCTTTCGCGGACGCGGACGGATTGCC
GTACCTGGAAGTCCATCACGTGCGCCGATTGGCGGAGCGAGGGCCCGACGTCATCTCGAACGCGGTAGCAATTTGCCCGA
ATTGCCACCGCGAATTGCATTACGGCGAAAAGGCGCGCGAGCTTGTCGAACGCCTCTATACTCGCGTGCCGCGTCTCGTT
CGCGTGTGA

Upstream 100 bases:

>100_bases
TCGATGCGACAAGTCGGCTTGCACGCCGGCGACGGCCGTTCCGAACTGTCCGCATGCTTATCTCGTAAAATCGCACACGG
ATTTTTGAGCGCCGTGATCG

Downstream 100 bases:

>100_bases
TCGAGCCGCACGCCTGGCAGCCGCGCGGAAGACGTCAGCCCGCAGCCCGCATCCGCGCCGCCACCACGAGCGAAATCAGG
CAGCCGGCCGCGAGATAACC

Product: 5-methylcytosine-specific restriction enzyme A

Products: NA

Alternate protein names: EcoKMcrA [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MPTSPTQAPISDEVSAPADGRPWTHEELRAAVDAYLEMLDSERAKRRYVKTEVYRALAERFHRTAKAFEYRMQNISYVLA
LMGRDWLPGLKPARNVGANGVLIEQFINEREYRTTAPVVEFELDVRDRLKRVPVAPPTGNARPEVGYATVAQVRRDPAVK
AWVLAQARGTCECCGNPAPFADADGLPYLEVHHVRRLAERGPDVISNAVAICPNCHRELHYGEKARELVERLYTRVPRLV
RV

Sequences:

>Translated_242_residues
MPTSPTQAPISDEVSAPADGRPWTHEELRAAVDAYLEMLDSERAKRRYVKTEVYRALAERFHRTAKAFEYRMQNISYVLA
LMGRDWLPGLKPARNVGANGVLIEQFINEREYRTTAPVVEFELDVRDRLKRVPVAPPTGNARPEVGYATVAQVRRDPAVK
AWVLAQARGTCECCGNPAPFADADGLPYLEVHHVRRLAERGPDVISNAVAICPNCHRELHYGEKARELVERLYTRVPRLV
RV
>Mature_241_residues
PTSPTQAPISDEVSAPADGRPWTHEELRAAVDAYLEMLDSERAKRRYVKTEVYRALAERFHRTAKAFEYRMQNISYVLAL
MGRDWLPGLKPARNVGANGVLIEQFINEREYRTTAPVVEFELDVRDRLKRVPVAPPTGNARPEVGYATVAQVRRDPAVKA
WVLAQARGTCECCGNPAPFADADGLPYLEVHHVRRLAERGPDVISNAVAICPNCHRELHYGEKARELVERLYTRVPRLVR
V

Specific function: Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG [H]

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787406, Length=115, Percent_Identity=50.4347826086956, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002711
- InterPro:   IPR003615 [H]

Pfam domain/function: PF01844 HNH [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 27370; Mature: 27238

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSPTQAPISDEVSAPADGRPWTHEELRAAVDAYLEMLDSERAKRRYVKTEVYRALAER
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHRTAKAFEYRMQNISYVLALMGRDWLPGLKPARNVGANGVLIEQFINEREYRTTAPVVE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
FELDVRDRLKRVPVAPPTGNARPEVGYATVAQVRRDPAVKAWVLAQARGTCECCGNPAPF
EECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCC
ADADGLPYLEVHHVRRLAERGPDVISNAVAICPNCHRELHYGEKARELVERLYTRVPRLV
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
RV
CC
>Mature Secondary Structure 
PTSPTQAPISDEVSAPADGRPWTHEELRAAVDAYLEMLDSERAKRRYVKTEVYRALAER
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHRTAKAFEYRMQNISYVLALMGRDWLPGLKPARNVGANGVLIEQFINEREYRTTAPVVE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
FELDVRDRLKRVPVAPPTGNARPEVGYATVAQVRRDPAVKAWVLAQARGTCECCGNPAPF
EECCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCCCCCC
ADADGLPYLEVHHVRRLAERGPDVISNAVAICPNCHRELHYGEKARELVERLYTRVPRLV
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
RV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1938927; 8905232; 9278503 [H]