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The map label for this gene is oprM [H]

Identifier: 189352914

GI number: 189352914

Start: 1060733

End: 1062181

Strand: Reverse

Name: oprM [H]

Synonym: BMULJ_04148

Alternate gene names: 189352914

Gene position: 1062181-1060733 (Counterclockwise)

Preceding gene: 189352915

Following gene: 189352913

Centisome position: 42.95

GC content: 70.74

Gene sequence:

>1449_bases
ATGCGATTGCTCATTCTCTCCGCTGCGGTTGCCGCCGCCCTCGCACTGTCGGCGTGCTCGATGGCGCCGAAACTGGTCAG
GCCCGCGATGCCGGTGCCGGGTACCTACACGGCCGCGACCGCGGCCGCCACCGCGCAGCATGCGAACGCAGCCGATCTCG
GCTGGCGCACGATGTTCGGCGATCGCCGCCTGCAGCGCCTGATCGAGCTCGCGCTCGACAACAACCGCGACCTGCGCCTC
GCTGCGCTGAACGTCCAGGCGGCCGAAGCGCAGTACGGCATTCAGCGCTCGGCGCGCCTGCCGTCCATCGGCGCCGGCGC
GAGCTTCACGCGCCAGCGCACGCCGGCCAACGCCGACATGAATCCGCCCGTGCCCGAGTCGATGCAGACGCAGTACGGCG
TGAACGTCGGGATCAGCGCGTTCGAGATCGATCTGTTCGGCCGTGTGAAATCGCTGTCCGATGCGGCGTTCGCGCGCTAC
CTCGCGACCGACCACGGCCGTCGCGCCGTGCAGATCGCGCTCGTCGGTTCCGTCGCGAACGCGTACTTCGCCGAGCGCCT
CGCGCAGGAACAGCGCGCGCTGTCGGAACGGACGCTGAACGACTGGCGGCAATCGCTCGATCTCGCGCGCCGGCTCAAGC
AGGCGAACCAGGCGAGCGGCGTCGACATCGCACAGGCCGAAGGCCAGGTCGCGAGCGCCGCCGCCGATCTCGAGGCGCGC
TCGCGCGCCGTCGAGCAGGCGCGCAACGCGCTGCGGCTACTGGTCGGCACCGAGCTGCCGAAGGACTTGCCCGAGCCGCT
GTCGCTCGAACGCCAGCCGGTACTGACGCAACTGCCGGCCGGGCTGCCGTCGGAACTGCTGTACCGCCGCCCGGACATCC
GGCAGGCCGAGCAGGCGCTCGTCGCGGCCAACGCCGATATCGGCGCGGCGCGCGCGGCATTCTTCCCGCGCCTGTCGCTG
ACGTCGACGATCGGCTTCCTGAGCCCGGCGATGGGCAGTCTGTTCGCCGGCGACCACCGTACCTGGGCGTTCGCGCCGCA
GATCACGGTGCCGATCTTCGAAGGCGGACGGCTGAGCGCCGAGCTGCGGCTCGCGGAGATCCGCAAGTCGAGCGCGGTCG
CCGAATACGAGCGCGCGATCCAGACGGCGTTCCGCGAAGTCGCCGACGGCCTCGCCGGGCGCGAGACCTTCAGCCGCCAG
ATCGATGCACAGCGCGACGTCGTCAAGAGCGCGGAGCGCCGCACCGACCTGGTGAACCTGCGCTATCGCGCGGGCATCGA
GGATCGCCTGGAACTGCTCGATTCGCAGCGGCAGCTGTATGCCGCGCGCCAGGCGCTGCTCGATCTGCGCAGCGCCGAGC
TCAGCAACGCAGTCGCGCTCTACAAGGCGCTCGGGGGCGGACTGACCGACACTGATGTCGCCGCGCCGAACGACGTGGCG
AAGGGCTGA

Upstream 100 bases:

>100_bases
TTCTTCGTGTTCGTGATGAGCGTGCAGGAACGTCTCTCGGCGTGGCTCGCATCGGGCAGGAAGCGCGCCGCCGTCACCCA
CGAACAAGAAGGTTGATGTC

Downstream 100 bases:

>100_bases
GCGGCGGGCGGTCGCCGTCGCGCAGTCGCTGCGACGCGCCGGCGGCGACCGCCCGGGATCCGCTTTCCTGCCGTCTCCCC
TTCGAGTGATTCCGATGAAC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MRLLILSAAVAAALALSACSMAPKLVRPAMPVPGTYTAATAAATAQHANAADLGWRTMFGDRRLQRLIELALDNNRDLRL
AALNVQAAEAQYGIQRSARLPSIGAGASFTRQRTPANADMNPPVPESMQTQYGVNVGISAFEIDLFGRVKSLSDAAFARY
LATDHGRRAVQIALVGSVANAYFAERLAQEQRALSERTLNDWRQSLDLARRLKQANQASGVDIAQAEGQVASAAADLEAR
SRAVEQARNALRLLVGTELPKDLPEPLSLERQPVLTQLPAGLPSELLYRRPDIRQAEQALVAANADIGAARAAFFPRLSL
TSTIGFLSPAMGSLFAGDHRTWAFAPQITVPIFEGGRLSAELRLAEIRKSSAVAEYERAIQTAFREVADGLAGRETFSRQ
IDAQRDVVKSAERRTDLVNLRYRAGIEDRLELLDSQRQLYAARQALLDLRSAELSNAVALYKALGGGLTDTDVAAPNDVA
KG

Sequences:

>Translated_482_residues
MRLLILSAAVAAALALSACSMAPKLVRPAMPVPGTYTAATAAATAQHANAADLGWRTMFGDRRLQRLIELALDNNRDLRL
AALNVQAAEAQYGIQRSARLPSIGAGASFTRQRTPANADMNPPVPESMQTQYGVNVGISAFEIDLFGRVKSLSDAAFARY
LATDHGRRAVQIALVGSVANAYFAERLAQEQRALSERTLNDWRQSLDLARRLKQANQASGVDIAQAEGQVASAAADLEAR
SRAVEQARNALRLLVGTELPKDLPEPLSLERQPVLTQLPAGLPSELLYRRPDIRQAEQALVAANADIGAARAAFFPRLSL
TSTIGFLSPAMGSLFAGDHRTWAFAPQITVPIFEGGRLSAELRLAEIRKSSAVAEYERAIQTAFREVADGLAGRETFSRQ
IDAQRDVVKSAERRTDLVNLRYRAGIEDRLELLDSQRQLYAARQALLDLRSAELSNAVALYKALGGGLTDTDVAAPNDVA
KG
>Mature_482_residues
MRLLILSAAVAAALALSACSMAPKLVRPAMPVPGTYTAATAAATAQHANAADLGWRTMFGDRRLQRLIELALDNNRDLRL
AALNVQAAEAQYGIQRSARLPSIGAGASFTRQRTPANADMNPPVPESMQTQYGVNVGISAFEIDLFGRVKSLSDAAFARY
LATDHGRRAVQIALVGSVANAYFAERLAQEQRALSERTLNDWRQSLDLARRLKQANQASGVDIAQAEGQVASAAADLEAR
SRAVEQARNALRLLVGTELPKDLPEPLSLERQPVLTQLPAGLPSELLYRRPDIRQAEQALVAANADIGAARAAFFPRLSL
TSTIGFLSPAMGSLFAGDHRTWAFAPQITVPIFEGGRLSAELRLAEIRKSSAVAEYERAIQTAFREVADGLAGRETFSRQ
IDAQRDVVKSAERRTDLVNLRYRAGIEDRLELLDSQRQLYAARQALLDLRSAELSNAVALYKALGGGLTDTDVAAPNDVA
KG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=41.4847161572052, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI2367348, Length=427, Percent_Identity=26.9320843091335, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52019; Mature: 52019

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLILSAAVAAALALSACSMAPKLVRPAMPVPGTYTAATAAATAQHANAADLGWRTMFG
CEEEEHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHCCHHHHHH
DRRLQRLIELALDNNRDLRLAALNVQAAEAQYGIQRSARLPSIGAGASFTRQRTPANADM
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCC
NPPVPESMQTQYGVNVGISAFEIDLFGRVKSLSDAAFARYLATDHGRRAVQIALVGSVAN
CCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
AYFAERLAQEQRALSERTLNDWRQSLDLARRLKQANQASGVDIAQAEGQVASAAADLEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHH
SRAVEQARNALRLLVGTELPKDLPEPLSLERQPVLTQLPAGLPSELLYRRPDIRQAEQAL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCHHHHHHHH
VAANADIGAARAAFFPRLSLTSTIGFLSPAMGSLFAGDHRTWAFAPQITVPIFEGGRLSA
HHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCH
ELRLAEIRKSSAVAEYERAIQTAFREVADGLAGRETFSRQIDAQRDVVKSAERRTDLVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYRAGIEDRLELLDSQRQLYAARQALLDLRSAELSNAVALYKALGGGLTDTDVAAPNDVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KG
CC
>Mature Secondary Structure
MRLLILSAAVAAALALSACSMAPKLVRPAMPVPGTYTAATAAATAQHANAADLGWRTMFG
CEEEEHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHCCHHHHHH
DRRLQRLIELALDNNRDLRLAALNVQAAEAQYGIQRSARLPSIGAGASFTRQRTPANADM
HHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCC
NPPVPESMQTQYGVNVGISAFEIDLFGRVKSLSDAAFARYLATDHGRRAVQIALVGSVAN
CCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHH
AYFAERLAQEQRALSERTLNDWRQSLDLARRLKQANQASGVDIAQAEGQVASAAADLEAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHH
SRAVEQARNALRLLVGTELPKDLPEPLSLERQPVLTQLPAGLPSELLYRRPDIRQAEQAL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHCCCCHHHHHHHH
VAANADIGAARAAFFPRLSLTSTIGFLSPAMGSLFAGDHRTWAFAPQITVPIFEGGRLSA
HHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCH
ELRLAEIRKSSAVAEYERAIQTAFREVADGLAGRETFSRQIDAQRDVVKSAERRTDLVNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYRAGIEDRLELLDSQRQLYAARQALLDLRSAELSNAVALYKALGGGLTDTDVAAPNDVA
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]