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The map label for this gene is hpaD [H]

Identifier: 189352901

GI number: 189352901

Start: 1048626

End: 1049474

Strand: Direct

Name: hpaD [H]

Synonym: BMULJ_04135

Alternate gene names: 189352901

Gene position: 1048626-1049474 (Clockwise)

Preceding gene: 189352900

Following gene: 189352902

Centisome position: 42.4

GC content: 64.19

Gene sequence:

>849_bases
ATGGGCAAACTGTCCCTCGCCGCGAAGATCACGCATGTGCCGTCGATGTACCTGTCGGAGCTGCCCGGCAAGCATCACGG
CTGCCGCGACGCGGCGATCCGCGGCCATCGGCTGATCGGCGAGCGCTGCCGCGCGCTCGGCGTCGACACGATCGTCGTGT
CGGACGTGCACTGGCTCGTCAATGCCGGCTATCACGTGAACTGCAATGCGCGCTTTGCCGGCACGTACACGAGCAATGAA
CTGCCGCATTTCATCCGCGACATGCATTACGCGTATCCGGGCAATCCGGCGCTCGGCCGGCTGATCGCCGAGACGGCGAG
CGCGCGCGGCATCGCGACGCGCGCGCACGAGATCGACAGCCTCGAGCTCGAATACGGGACGCTCGTGCCGATGCGCTACA
TGAACGGCGACCAGCACTTCAAGGTCGTGTCGATCGCCGGCTGGTGCATGTGGCACACGCTCGACGACAGTCGCCGCTTC
GGCGAAGCGCTGCTCGAAGCGATCGAAAAGAGCGATTCGAACGTCGCGTTCCTCGCGAGCGGTTCGCTGTCGCATCGCTT
CAACGACAACGGCAGCCCCGAGGAATCGATTCACGAGATCAGCCGCGAATTTTTCCGGCAGGTCGATCTGCGCGTCGTCG
AACTGTGGAAGCAGGGCGACTTCAGGACGTTCTGCGCGATGCTGCCCGAGTACAACCAGCACTGCCACGGCGAAGGCGGG
ATGCACGACACCGCGATGCTGCTCGGCCTGCTCGGCTGGGATCGCTACGACAAGCCGGTCGAAATCGTCACCGACTACTT
CGCGAGTTCGGGCACCGGCCAGATCAACGCCATTTTTCCGCTGCGCTGA

Upstream 100 bases:

>100_bases
AGTCAAGAACGTCTGCATCTCGATGGGCTCGCATCACATTCCGCGCTGGGGCGTCTGACGCGCGCCGTTGCGCCGCGACA
AGAACGATACGGAGACTACG

Downstream 100 bases:

>100_bases
ACGCGCGCGCATCCGACATCCGACCGGAGACTGCCATGCCGCACATCGTCGTCGAATATACGGCGAACATCCGCGCCGAC
GCCCGCATCCCCGAGCTGCT

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MGKLSLAAKITHVPSMYLSELPGKHHGCRDAAIRGHRLIGERCRALGVDTIVVSDVHWLVNAGYHVNCNARFAGTYTSNE
LPHFIRDMHYAYPGNPALGRLIAETASARGIATRAHEIDSLELEYGTLVPMRYMNGDQHFKVVSIAGWCMWHTLDDSRRF
GEALLEAIEKSDSNVAFLASGSLSHRFNDNGSPEESIHEISREFFRQVDLRVVELWKQGDFRTFCAMLPEYNQHCHGEGG
MHDTAMLLGLLGWDRYDKPVEIVTDYFASSGTGQINAIFPLR

Sequences:

>Translated_282_residues
MGKLSLAAKITHVPSMYLSELPGKHHGCRDAAIRGHRLIGERCRALGVDTIVVSDVHWLVNAGYHVNCNARFAGTYTSNE
LPHFIRDMHYAYPGNPALGRLIAETASARGIATRAHEIDSLELEYGTLVPMRYMNGDQHFKVVSIAGWCMWHTLDDSRRF
GEALLEAIEKSDSNVAFLASGSLSHRFNDNGSPEESIHEISREFFRQVDLRVVELWKQGDFRTFCAMLPEYNQHCHGEGG
MHDTAMLLGLLGWDRYDKPVEIVTDYFASSGTGQINAIFPLR
>Mature_281_residues
GKLSLAAKITHVPSMYLSELPGKHHGCRDAAIRGHRLIGERCRALGVDTIVVSDVHWLVNAGYHVNCNARFAGTYTSNEL
PHFIRDMHYAYPGNPALGRLIAETASARGIATRAHEIDSLELEYGTLVPMRYMNGDQHFKVVSIAGWCMWHTLDDSRRFG
EALLEAIEKSDSNVAFLASGSLSHRFNDNGSPEESIHEISREFFRQVDLRVVELWKQGDFRTFCAMLPEYNQHCHGEGGM
HDTAMLLGLLGWDRYDKPVEIVTDYFASSGTGQINAIFPLR

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 31620; Mature: 31489

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLSLAAKITHVPSMYLSELPGKHHGCRDAAIRGHRLIGERCRALGVDTIVVSDVHWLV
CCCCCHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
NAGYHVNCNARFAGTYTSNELPHFIRDMHYAYPGNPALGRLIAETASARGIATRAHEIDS
CCCEEEECCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCC
LELEYGTLVPMRYMNGDQHFKVVSIAGWCMWHTLDDSRRFGEALLEAIEKSDSNVAFLAS
EEECCCCEEEEEECCCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEC
GSLSHRFNDNGSPEESIHEISREFFRQVDLRVVELWKQGDFRTFCAMLPEYNQHCHGEGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCC
MHDTAMLLGLLGWDRYDKPVEIVTDYFASSGTGQINAIFPLR
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure 
GKLSLAAKITHVPSMYLSELPGKHHGCRDAAIRGHRLIGERCRALGVDTIVVSDVHWLV
CCCCHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
NAGYHVNCNARFAGTYTSNELPHFIRDMHYAYPGNPALGRLIAETASARGIATRAHEIDS
CCCEEEECCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCC
LELEYGTLVPMRYMNGDQHFKVVSIAGWCMWHTLDDSRRFGEALLEAIEKSDSNVAFLAS
EEECCCCEEEEEECCCCCCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEC
GSLSHRFNDNGSPEESIHEISREFFRQVDLRVVELWKQGDFRTFCAMLPEYNQHCHGEGG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHCCCCCCC
MHDTAMLLGLLGWDRYDKPVEIVTDYFASSGTGQINAIFPLR
CHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]