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The map label for this gene is ald [H]

Identifier: 189352888

GI number: 189352888

Start: 1032525

End: 1033640

Strand: Reverse

Name: ald [H]

Synonym: BMULJ_04122

Alternate gene names: 189352888

Gene position: 1033640-1032525 (Counterclockwise)

Preceding gene: 189352894

Following gene: 189352883

Centisome position: 41.79

GC content: 69.98

Gene sequence:

>1116_bases
ATGCTGATCGGTGTGCCGAAAGAGATCAAGAACCACGAATACCGCGTCGGCCTGACGCCCGCCGGTGCACGCGAGCTCAC
GCGGCATGGCCACAAGGTGCTCGTGCAGCGCGGTGCGGGCACCGCGATCGGCCTGCTCGACGACGACTACGCCGCCGCCG
GCGCAGCCCTGTGCGACGGCGCCGAGGAAGTGTTTGCGCGCGCCGACATGATCGTCAAGGTGAAGGAGCCGCAGCCCGCC
GAGTGCGCGATGCTGCGGCGCGGGCAGCTTCTGTACGCCTATCTGCATCTCGCGCCCGATCCTGAACAGGCGGCCCCACT
CGTGCAGTCGGGCGCCGTGTGCATCGCCTACGAAACCGTGACCGGGCCCGGCGGCGGCCTGCCGCTGCTCGCGCCGATGA
GCGAGGTCGCCGGCCGCATGTCGATCCAGGTCGCGGCCACGCATCTCGAAAGTCCGCGCGGCGGGCGCGGCATGCTGATG
GCGGGCGTGCCCGGCGTGCCGGCCGCGCACGTCGTCGTGCTCGGCGCGGGCGTCGTCGGCACCGGCGCCTTGCAGATGGC
GGTCGGCCTCGGCGCGCGCGTGACGGTGCTCGACACCAACGTGAACCGGCTGCGGCAACTCGATTTGATCTTCGCGAACC
GGATCGCGACCGTCTGCTCGAATGCGCAGACGATCGACGAAGCGATTCGCGATGCGGACGTCGTGATCGGCGCGGTGCTG
GTGCCCGGTGCGTCCGCGCCGAAGCTCGTCACGCGCGACATGATCGCGACGATGCGGGCCGGCGCGGTCGTCGTCGACGT
CGCGATCGATCAGGGCGGCTGCTTCGAGACCTCGCATCCGACCACGCACGCGGATCCGACCTTCGTCGTCGACGGCGTCG
TTCACTACTGCGTCGCGAACATGCCGGGCGCCGTCGCGCGCACGTCGACGTTCGCGCTGAACAACGCGACGCTCGGCCAC
GCACTCGCGCTCGCCGACAAGGGCTGGAAACAGGCGATGATCGACGATCCGCATCTGCGCGCGGGGCTGAACGTCTGCGA
CGGGCACATCACCTACGAAGCAGTTGCACAGGCGCTGCGGCTGCCGTACGTGCCGGCGATCGACGTGCTCGCGTGA

Upstream 100 bases:

>100_bases
CGACGGAACGCGCGTCGCACAACGACACGCGCGAACGCAACTGCATCGCCCCATCGATCCGCGGCACGGCGACGATAACG
ACAACCGTTGGAGACGAGTC

Downstream 100 bases:

>100_bases
TCGCGTGATCGCGTGATCGCGCGACGGCGTGGCACGCGTCGACTGGTCGCGTTCACGTGCCGTTGCGGCGGCGGCGCTGC
GCCGCGCTTGCGTCCGCGGC

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MLIGVPKEIKNHEYRVGLTPAGARELTRHGHKVLVQRGAGTAIGLLDDDYAAAGAALCDGAEEVFARADMIVKVKEPQPA
ECAMLRRGQLLYAYLHLAPDPEQAAPLVQSGAVCIAYETVTGPGGGLPLLAPMSEVAGRMSIQVAATHLESPRGGRGMLM
AGVPGVPAAHVVVLGAGVVGTGALQMAVGLGARVTVLDTNVNRLRQLDLIFANRIATVCSNAQTIDEAIRDADVVIGAVL
VPGASAPKLVTRDMIATMRAGAVVVDVAIDQGGCFETSHPTTHADPTFVVDGVVHYCVANMPGAVARTSTFALNNATLGH
ALALADKGWKQAMIDDPHLRAGLNVCDGHITYEAVAQALRLPYVPAIDVLA

Sequences:

>Translated_371_residues
MLIGVPKEIKNHEYRVGLTPAGARELTRHGHKVLVQRGAGTAIGLLDDDYAAAGAALCDGAEEVFARADMIVKVKEPQPA
ECAMLRRGQLLYAYLHLAPDPEQAAPLVQSGAVCIAYETVTGPGGGLPLLAPMSEVAGRMSIQVAATHLESPRGGRGMLM
AGVPGVPAAHVVVLGAGVVGTGALQMAVGLGARVTVLDTNVNRLRQLDLIFANRIATVCSNAQTIDEAIRDADVVIGAVL
VPGASAPKLVTRDMIATMRAGAVVVDVAIDQGGCFETSHPTTHADPTFVVDGVVHYCVANMPGAVARTSTFALNNATLGH
ALALADKGWKQAMIDDPHLRAGLNVCDGHITYEAVAQALRLPYVPAIDVLA
>Mature_371_residues
MLIGVPKEIKNHEYRVGLTPAGARELTRHGHKVLVQRGAGTAIGLLDDDYAAAGAALCDGAEEVFARADMIVKVKEPQPA
ECAMLRRGQLLYAYLHLAPDPEQAAPLVQSGAVCIAYETVTGPGGGLPLLAPMSEVAGRMSIQVAATHLESPRGGRGMLM
AGVPGVPAAHVVVLGAGVVGTGALQMAVGLGARVTVLDTNVNRLRQLDLIFANRIATVCSNAQTIDEAIRDADVVIGAVL
VPGASAPKLVTRDMIATMRAGAVVVDVAIDQGGCFETSHPTTHADPTFVVDGVVHYCVANMPGAVARTSTFALNNATLGH
ALALADKGWKQAMIDDPHLRAGLNVCDGHITYEAVAQALRLPYVPAIDVLA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=30.3468208092486, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=30.3468208092486, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1787887, Length=382, Percent_Identity=29.8429319371728, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550456, Length=372, Percent_Identity=26.8817204301075, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38494; Mature: 38494

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGVPKEIKNHEYRVGLTPAGARELTRHGHKVLVQRGAGTAIGLLDDDYAAAGAALCDG
CCCCCCHHHCCCCEEEECCCCCHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHHHCC
AEEVFARADMIVKVKEPQPAECAMLRRGQLLYAYLHLAPDPEQAAPLVQSGAVCIAYETV
HHHHHHHCCEEEEECCCCCHHHHHHHCCCEEEEEEEECCCHHHHCHHHHCCCEEEEEEEE
TGPGGGLPLLAPMSEVAGRMSIQVAATHLESPRGGRGMLMAGVPGVPAAHVVVLGAGVVG
CCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCCC
TGALQMAVGLGARVTVLDTNVNRLRQLDLIFANRIATVCSNAQTIDEAIRDADVVIGAVL
CCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEE
VPGASAPKLVTRDMIATMRAGAVVVDVAIDQGGCFETSHPTTHADPTFVVDGVVHYCVAN
ECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLGHALALADKGWKQAMIDDPHLRAGLNVCDGHITYEAVAQALR
CCCCHHCCEEEEECCCCHHHHEEECCCCCHHHCCCCCHHHCCCCCCCCCEEHHHHHHHHH
LPYVPAIDVLA
CCCCCCHHHCC
>Mature Secondary Structure
MLIGVPKEIKNHEYRVGLTPAGARELTRHGHKVLVQRGAGTAIGLLDDDYAAAGAALCDG
CCCCCCHHHCCCCEEEECCCCCHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHHHCC
AEEVFARADMIVKVKEPQPAECAMLRRGQLLYAYLHLAPDPEQAAPLVQSGAVCIAYETV
HHHHHHHCCEEEEECCCCCHHHHHHHCCCEEEEEEEECCCHHHHCHHHHCCCEEEEEEEE
TGPGGGLPLLAPMSEVAGRMSIQVAATHLESPRGGRGMLMAGVPGVPAAHVVVLGAGVVG
CCCCCCCCCCCCHHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEEEEECCCCC
TGALQMAVGLGARVTVLDTNVNRLRQLDLIFANRIATVCSNAQTIDEAIRDADVVIGAVL
CCHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEE
VPGASAPKLVTRDMIATMRAGAVVVDVAIDQGGCFETSHPTTHADPTFVVDGVVHYCVAN
ECCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
MPGAVARTSTFALNNATLGHALALADKGWKQAMIDDPHLRAGLNVCDGHITYEAVAQALR
CCCCHHCCEEEEECCCCHHHHEEECCCCCHHHCCCCCHHHCCCCCCCCCEEHHHHHHHHH
LPYVPAIDVLA
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]