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The map label for this gene is rfbX [H]

Identifier: 189352871

GI number: 189352871

Start: 1009025

End: 1010278

Strand: Direct

Name: rfbX [H]

Synonym: BMULJ_04105

Alternate gene names: 189352871

Gene position: 1009025-1010278 (Clockwise)

Preceding gene: 189352870

Following gene: 189352874

Centisome position: 40.8

GC content: 66.43

Gene sequence:

>1254_bases
ATGACCGCCATTCGCAAAAATTTCGCGATGCTGTTCGCGTTGCAAATCTCGACCTATGTCGTGCCGCTCGTCACGCTGCC
GCTGCTTACGCGCGCGCTCGGCCCGCAGCAGTATGGACGGCTGTCGTTCGTGCTCGCGGTGACGACGTACTTCATCAATC
TGGTCAACTACAGCTTCGACCTGACGGCCACGCCGCGCGTCGCGCTCGCGCGCGACAAGCTCGAGCGTTCGCGAATCTTC
TGGACGACCGTCACCGCGCAGTGGCTGATCGCGATCGCGGGCCTCGGCGTGCTGATTGCGCTGACGCTGGCGATTCCGCA
CTTCGCGGCGGAGCGCACGCCGCTGCTGATCGGGTTCGGCATGGCCGTGGGCGCCGCGCTTACGCCCGGCTGGTACTTTC
AGGGGATCCAGAAGCTCAGCGTCTACAGCATGACGGTCGTCGTGTATCGCGCGCTGAGCGTCGTCGCGTTCTTCCTCTGG
GTCCACACGCCGGACGACATTCTCCCGGCGATCGCCATCAACTCGGCCGTGCCGCTGCTGTGCGGCGTGACGCTGCTCGC
CTATCTGTTCGTCTGCCGCGAAGTCGCGCGCGTGCGCGTGCGGCTCGCCGACATCGTCACTGCCGTGAAGGGCGGCACGC
AGGTCTTTCTCGCGTCGACGTCGATTTCGTTCTACGCGTCGACCAACACCGTGCTGCTGAGCATGGTGGCCGGCAACGTC
GCGGCAGGCTACTTTGCCGCCGGCGACAAGCTGATCCGCGCGGCCGTCGGCATGCTGCAGCCGCTGCGGGCGACCACCTA
TCCGCACATCACGAACCTGATGCACCACGCGCGCGACGAGGCGTTCGCGTTCCTGCGCAAGCTGATCGTCTGGCAGGGCG
TGCTCGTGCTCGCGATGTCGGCGACGATCTACGCATTCGCGCCGATGGCGGTCCGGATTCTTTATGGCGCCTCGTTCGAG
CCGACCGTCGCGGTGCTGCGCTGTCTCGCGCTGGTGCCGTTCATGGCCTGCATGACCGATCTGTTCGGCGTTCAGACGAT
GCTGCCGCTCGGGATGAAGCGCGCGTTCAGCACGATCCTGATCTCGTCGGGGCTGCTGAACGTGACGATCCTGCCGCCGC
TCGCGGCGCTGTTCGCGGAACGCGGGGCGGCCATCGCCGTGCTGCTGGTCGAGACGGCCGTGGTCGGGGCGCTCGTGTAC
GTGCTGTACCGCGAACGGGTCGGCCTGATCGATATGCCGGCGCGCTCGCGGTAA

Upstream 100 bases:

>100_bases
GCCTCGTCGCTGTACGCCGTACTCGGCATCGACGCGTCGGCGCCGCATCCGCACGCCCATTGACAGTCGGCGCACGTTCA
CTCTCAGCCCAGAAGGCACG

Downstream 100 bases:

>100_bases
CGCGATTCGCTGATCGGCCGTCGTTACGATCGACGAATCCGGGGCGGCGCCGGTCGATGTCTTCGGCCTGGCTTGCGAGG
CTTCTGCAACCGGACGCAAC

Product: PST family polysaccharide transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTAIRKNFAMLFALQISTYVVPLVTLPLLTRALGPQQYGRLSFVLAVTTYFINLVNYSFDLTATPRVALARDKLERSRIF
WTTVTAQWLIAIAGLGVLIALTLAIPHFAAERTPLLIGFGMAVGAALTPGWYFQGIQKLSVYSMTVVVYRALSVVAFFLW
VHTPDDILPAIAINSAVPLLCGVTLLAYLFVCREVARVRVRLADIVTAVKGGTQVFLASTSISFYASTNTVLLSMVAGNV
AAGYFAAGDKLIRAAVGMLQPLRATTYPHITNLMHHARDEAFAFLRKLIVWQGVLVLAMSATIYAFAPMAVRILYGASFE
PTVAVLRCLALVPFMACMTDLFGVQTMLPLGMKRAFSTILISSGLLNVTILPPLAALFAERGAAIAVLLVETAVVGALVY
VLYRERVGLIDMPARSR

Sequences:

>Translated_417_residues
MTAIRKNFAMLFALQISTYVVPLVTLPLLTRALGPQQYGRLSFVLAVTTYFINLVNYSFDLTATPRVALARDKLERSRIF
WTTVTAQWLIAIAGLGVLIALTLAIPHFAAERTPLLIGFGMAVGAALTPGWYFQGIQKLSVYSMTVVVYRALSVVAFFLW
VHTPDDILPAIAINSAVPLLCGVTLLAYLFVCREVARVRVRLADIVTAVKGGTQVFLASTSISFYASTNTVLLSMVAGNV
AAGYFAAGDKLIRAAVGMLQPLRATTYPHITNLMHHARDEAFAFLRKLIVWQGVLVLAMSATIYAFAPMAVRILYGASFE
PTVAVLRCLALVPFMACMTDLFGVQTMLPLGMKRAFSTILISSGLLNVTILPPLAALFAERGAAIAVLLVETAVVGALVY
VLYRERVGLIDMPARSR
>Mature_416_residues
TAIRKNFAMLFALQISTYVVPLVTLPLLTRALGPQQYGRLSFVLAVTTYFINLVNYSFDLTATPRVALARDKLERSRIFW
TTVTAQWLIAIAGLGVLIALTLAIPHFAAERTPLLIGFGMAVGAALTPGWYFQGIQKLSVYSMTVVVYRALSVVAFFLWV
HTPDDILPAIAINSAVPLLCGVTLLAYLFVCREVARVRVRLADIVTAVKGGTQVFLASTSISFYASTNTVLLSMVAGNVA
AGYFAAGDKLIRAAVGMLQPLRATTYPHITNLMHHARDEAFAFLRKLIVWQGVLVLAMSATIYAFAPMAVRILYGASFEP
TVAVLRCLALVPFMACMTDLFGVQTMLPLGMKRAFSTILISSGLLNVTILPPLAALFAERGAAIAVLLVETAVVGALVYV
LYRERVGLIDMPARSR

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=393, Percent_Identity=29.7709923664122, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 45259; Mature: 45128

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIRKNFAMLFALQISTYVVPLVTLPLLTRALGPQQYGRLSFVLAVTTYFINLVNYSFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEE
LTATPRVALARDKLERSRIFWTTVTAQWLIAIAGLGVLIALTLAIPHFAAERTPLLIGFG
CCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
MAVGAALTPGWYFQGIQKLSVYSMTVVVYRALSVVAFFLWVHTPDDILPAIAINSAVPLL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
CGVTLLAYLFVCREVARVRVRLADIVTAVKGGTQVFLASTSISFYASTNTVLLSMVAGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHHH
AAGYFAAGDKLIRAAVGMLQPLRATTYPHITNLMHHARDEAFAFLRKLIVWQGVLVLAMS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIYAFAPMAVRILYGASFEPTVAVLRCLALVPFMACMTDLFGVQTMLPLGMKRAFSTIL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLLNVTILPPLAALFAERGAAIAVLLVETAVVGALVYVLYRERVGLIDMPARSR
HHCCCCEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
TAIRKNFAMLFALQISTYVVPLVTLPLLTRALGPQQYGRLSFVLAVTTYFINLVNYSFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEE
LTATPRVALARDKLERSRIFWTTVTAQWLIAIAGLGVLIALTLAIPHFAAERTPLLIGFG
CCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
MAVGAALTPGWYFQGIQKLSVYSMTVVVYRALSVVAFFLWVHTPDDILPAIAINSAVPLL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
CGVTLLAYLFVCREVARVRVRLADIVTAVKGGTQVFLASTSISFYASTNTVLLSMVAGNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCHHHHHHHHHHHH
AAGYFAAGDKLIRAAVGMLQPLRATTYPHITNLMHHARDEAFAFLRKLIVWQGVLVLAMS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIYAFAPMAVRILYGASFEPTVAVLRCLALVPFMACMTDLFGVQTMLPLGMKRAFSTIL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISSGLLNVTILPPLAALFAERGAAIAVLLVETAVVGALVYVLYRERVGLIDMPARSR
HHCCCCEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]