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The map label for this gene is gph [H]

Identifier: 189352863

GI number: 189352863

Start: 994492

End: 995166

Strand: Reverse

Name: gph [H]

Synonym: BMULJ_04097

Alternate gene names: 189352863

Gene position: 995166-994492 (Counterclockwise)

Preceding gene: 189352864

Following gene: 189352862

Centisome position: 40.24

GC content: 67.56

Gene sequence:

>675_bases
ATGCGCATCGAAACGTCATTTCTGTTCGATCTCGACGGTACGCTGGTCGACAGCGTCTATCAGCACGTGCTCGCGTGGAA
GGAAGCGCTGGATGCCGAAGGTCTCGAGCTGTCCGTGTGGCGCATCCACCGGAAAATCGGCATGAGCGGCGGCCTCTTTC
TGAACCAGCTGTTGCGCGAAACGTCGGAAAACATCGACACCGATCGCATCGAGCGGCTCGCGCGCCTGCATGCGGCCGCC
TACCAGCGGCCGCGCGCGCAGGTCCGCCCGCTGCCCGGCGCGACCGAACTGCTGAACTCGCTTTCCGCCGCCGGCATCCG
CTGGGCGATCGCGACGAGCGGCCGGATGGAGACGGCGAGCGTCAACCTCGACGCGCTCGGCGTCGACGCGACGAAGGCCG
TCGTCGTGACGCGCGACCAGGTCAAGTATGCGAAACCCGACCCCGACCTGTTCGTCGAAGCCGCGCGCCGGCTCAACGTG
CCGATCGAGCACACGGTCGTCGTCGGCGACAGCATCTGGGACATGCTCGCCGCCTGCCGCTGTCGCGCGCTCGGCGTCGG
ACTGCGCTCGGGCGGCTATGGCACCGAGGAACTGCAAGACGCCGGTGCACTGCGCGTCTATGACGACCCGGCGGACCTGC
TCTGGCATCTCGACGAAATCGCCGCGCGGCCGTAG

Upstream 100 bases:

>100_bases
CGCGCGCGCGCTCGCGCGGCCGCGATGAACGCACGACTACAATGGCATCTGGCCCATCATTCGCGGCTTGCTTGCGCGAA
ACCGAAACAGGGAGATTCAG

Downstream 100 bases:

>100_bases
GTGCGTCGCGCGGTCGTTCGATGCCGTGCGGGCGCCGGTTGTCGGCGTGCAACGGTACGCGCGACAGCGCGTATCAAGAG
CTGCATATGCCTAAGGCAGA

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRIETSFLFDLDGTLVDSVYQHVLAWKEALDAEGLELSVWRIHRKIGMSGGLFLNQLLRETSENIDTDRIERLARLHAAA
YQRPRAQVRPLPGATELLNSLSAAGIRWAIATSGRMETASVNLDALGVDATKAVVVTRDQVKYAKPDPDLFVEAARRLNV
PIEHTVVVGDSIWDMLAACRCRALGVGLRSGGYGTEELQDAGALRVYDDPADLLWHLDEIAARP

Sequences:

>Translated_224_residues
MRIETSFLFDLDGTLVDSVYQHVLAWKEALDAEGLELSVWRIHRKIGMSGGLFLNQLLRETSENIDTDRIERLARLHAAA
YQRPRAQVRPLPGATELLNSLSAAGIRWAIATSGRMETASVNLDALGVDATKAVVVTRDQVKYAKPDPDLFVEAARRLNV
PIEHTVVVGDSIWDMLAACRCRALGVGLRSGGYGTEELQDAGALRVYDDPADLLWHLDEIAARP
>Mature_224_residues
MRIETSFLFDLDGTLVDSVYQHVLAWKEALDAEGLELSVWRIHRKIGMSGGLFLNQLLRETSENIDTDRIERLARLHAAA
YQRPRAQVRPLPGATELLNSLSAAGIRWAIATSGRMETASVNLDALGVDATKAVVVTRDQVKYAKPDPDLFVEAARRLNV
PIEHTVVVGDSIWDMLAACRCRALGVGLRSGGYGTEELQDAGALRVYDDPADLLWHLDEIAARP

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=184, Percent_Identity=35.3260869565217, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24701; Mature: 24701

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIETSFLFDLDGTLVDSVYQHVLAWKEALDAEGLELSVWRIHRKIGMSGGLFLNQLLRE
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TSENIDTDRIERLARLHAAAYQRPRAQVRPLPGATELLNSLSAAGIRWAIATSGRMETAS
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCEEEEE
VNLDALGVDATKAVVVTRDQVKYAKPDPDLFVEAARRLNVPIEHTVVVGDSIWDMLAACR
ECCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
CRALGVGLRSGGYGTEELQDAGALRVYDDPADLLWHLDEIAARP
HHHHCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIETSFLFDLDGTLVDSVYQHVLAWKEALDAEGLELSVWRIHRKIGMSGGLFLNQLLRE
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
TSENIDTDRIERLARLHAAAYQRPRAQVRPLPGATELLNSLSAAGIRWAIATSGRMETAS
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCEEEEE
VNLDALGVDATKAVVVTRDQVKYAKPDPDLFVEAARRLNVPIEHTVVVGDSIWDMLAACR
ECCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHH
CRALGVGLRSGGYGTEELQDAGALRVYDDPADLLWHLDEIAARP
HHHHCCCCCCCCCCHHHHHCCCCEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]