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The map label for this gene is gsiB [H]

Identifier: 189352849

GI number: 189352849

Start: 974869

End: 976431

Strand: Direct

Name: gsiB [H]

Synonym: BMULJ_04083

Alternate gene names: 189352849

Gene position: 974869-976431 (Clockwise)

Preceding gene: 189352848

Following gene: 189352850

Centisome position: 39.42

GC content: 64.56

Gene sequence:

>1563_bases
ATGAACAAGTCGCTTTCGTTGCCGATGTTCCGTCCGCGCGCGCTGCTCGCCGCCGGCGCGAGCGTGTTCGCGCTGACGGC
CGCGCTGCCCGCGTTCGCGCAGCAGACCGCGGTGATCGCGGTCGACCAGACGTTTACGACGATGGATCCGTACGACGCGA
ACGACACGGTGTCGCAGGCGGCCGCGAAGTCGTTCTACGAGGGCTTGTTCGGCTTCGACAAGGACATGAAGGTCGTGAAC
GTGCTCGCGACCGGCTACGACGCGTCGCCGGACGCGAAGGTCTACACGATCCATCTGCGCAAGGGCGTGAAGTTCCATGA
CGGCACCGACTTCAACGCGGACGCGGTGAAGGCGAACTTCGACCGCGTGACCGATCCGGCGAACAAGCTCAAGCGCTACA
ACCTGTTCCGCGTGATCGAGAAGACCGAAGTCGTCGATCCGTACACGGTGAAGCTCACGCTGCGCGAGCCGTTCTCCGCG
ATCATCAACACGCTCGCGCATCCGTCGGCCGTGATGATTTCGCCGGCCGCGCTGAAGAAGTGGGGGCGCGACGTCGCGCT
GCATCCGGTCGGCACCGGGCCGTTCGAGTTCGTCGAATGGAAGCAGACGGACGACCTGAAGGTGAAGAAGTTCGCCGGCT
ACTGGAAGAAGGGCTATCCGAAGATCGACGCGCTCGACTGGAAACCCGTGATCGACAACAACACGCGCGCCGCGCTGATG
AAGGCCGGACAGGCCGACATGGCGTTCCGCATTCCGTACGAGCAGGTGCCCGACCTGAAGAGCAATCCGAAGCTCGACAT
CGTCGAGCGGCCGTCGATCGTCGCGCGCTACGTGTCGCTGAACATGCAGCAAAAGCCGTTCGACAACCTGAAGGTGCGCC
AGGCGCTGAACTACGCGATCAACAAGGAGGCGCTCGTGAAGGTCGCGTTCTCGGGCTTCGCGACGCCGTCGACGGGCGTC
GTGCCGCCGGGCGTGGAATTCTCGACGAAGACGGGCCCGTGGCCGTACGATCCGGCGAAGGCGCGCGCGCTGCTGAAGGA
GGCCGGCTATCCGAACGGCTTCGAGACGGTGCTCTGGTCCGGCTACAACAACACGACGTCGCAGAAGGTGATCCAGTTCC
TGCAGCAGCAACTCGCGCAGGTGGGCGTGAAGGCGCAGGTGCAGGCGCTCGAATCGGGTGAGCGCGTGTCGAAGGTCGAA
AGCGCGCCGGATCCGGCCACCGCGCCGGTGCGGATGTTCTATATCGGCTGGTCCGCGTCGACCGGCGAAGCGAACTGGGC
GCTCACGCCGCTGTTGGCGGCGGCGTCGATGCCGCCGAAGCTGTTCAATACGGCGTACTACCGGAACCCGGCCCTCGACG
ACGACCTCTCGAAGGCGCTGCAGACCGTCGATCGCGCGAAAAAGGCCGAGCTCTACGGCGATGCGCAGAAGCGGATCTGG
GCCGACGCGCCGTGGATCTTCCTCGTGCAGGAGAACATCGTCTATGCGCGCAGCAAGCGGCTGCACGGCATCTACGTGAT
GCCGGACGGGTCGTTCAATATCGACGAGGTCTCGATGAACTGA

Upstream 100 bases:

>100_bases
CGTCGCGCGTTTGCGAGGCAATGTGTTTGTCCGGCGCGGGGCAGTCGATCCCGCGTCTTCCGTCACGCGTCACAGAACCA
TGCACAAGGAGCCAGCCTTT

Downstream 100 bases:

>100_bases
GCGCGCTCGCGCGCATCGCGGCTTGCCGGCGCAGCGCGTCGATCGCGCCGGCAAGCGGCCCCTGTCCGGTGTCCCCATGC
TGACTTTCATCATCAAACGG

Product: peptide/nickel transport system substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNKSLSLPMFRPRALLAAGASVFALTAALPAFAQQTAVIAVDQTFTTMDPYDANDTVSQAAAKSFYEGLFGFDKDMKVVN
VLATGYDASPDAKVYTIHLRKGVKFHDGTDFNADAVKANFDRVTDPANKLKRYNLFRVIEKTEVVDPYTVKLTLREPFSA
IINTLAHPSAVMISPAALKKWGRDVALHPVGTGPFEFVEWKQTDDLKVKKFAGYWKKGYPKIDALDWKPVIDNNTRAALM
KAGQADMAFRIPYEQVPDLKSNPKLDIVERPSIVARYVSLNMQQKPFDNLKVRQALNYAINKEALVKVAFSGFATPSTGV
VPPGVEFSTKTGPWPYDPAKARALLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLAQVGVKAQVQALESGERVSKVE
SAPDPATAPVRMFYIGWSASTGEANWALTPLLAAASMPPKLFNTAYYRNPALDDDLSKALQTVDRAKKAELYGDAQKRIW
ADAPWIFLVQENIVYARSKRLHGIYVMPDGSFNIDEVSMN

Sequences:

>Translated_520_residues
MNKSLSLPMFRPRALLAAGASVFALTAALPAFAQQTAVIAVDQTFTTMDPYDANDTVSQAAAKSFYEGLFGFDKDMKVVN
VLATGYDASPDAKVYTIHLRKGVKFHDGTDFNADAVKANFDRVTDPANKLKRYNLFRVIEKTEVVDPYTVKLTLREPFSA
IINTLAHPSAVMISPAALKKWGRDVALHPVGTGPFEFVEWKQTDDLKVKKFAGYWKKGYPKIDALDWKPVIDNNTRAALM
KAGQADMAFRIPYEQVPDLKSNPKLDIVERPSIVARYVSLNMQQKPFDNLKVRQALNYAINKEALVKVAFSGFATPSTGV
VPPGVEFSTKTGPWPYDPAKARALLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLAQVGVKAQVQALESGERVSKVE
SAPDPATAPVRMFYIGWSASTGEANWALTPLLAAASMPPKLFNTAYYRNPALDDDLSKALQTVDRAKKAELYGDAQKRIW
ADAPWIFLVQENIVYARSKRLHGIYVMPDGSFNIDEVSMN
>Mature_520_residues
MNKSLSLPMFRPRALLAAGASVFALTAALPAFAQQTAVIAVDQTFTTMDPYDANDTVSQAAAKSFYEGLFGFDKDMKVVN
VLATGYDASPDAKVYTIHLRKGVKFHDGTDFNADAVKANFDRVTDPANKLKRYNLFRVIEKTEVVDPYTVKLTLREPFSA
IINTLAHPSAVMISPAALKKWGRDVALHPVGTGPFEFVEWKQTDDLKVKKFAGYWKKGYPKIDALDWKPVIDNNTRAALM
KAGQADMAFRIPYEQVPDLKSNPKLDIVERPSIVARYVSLNMQQKPFDNLKVRQALNYAINKEALVKVAFSGFATPSTGV
VPPGVEFSTKTGPWPYDPAKARALLKEAGYPNGFETVLWSGYNNTTSQKVIQFLQQQLAQVGVKAQVQALESGERVSKVE
SAPDPATAPVRMFYIGWSASTGEANWALTPLLAAASMPPKLFNTAYYRNPALDDDLSKALQTVDRAKKAELYGDAQKRIW
ADAPWIFLVQENIVYARSKRLHGIYVMPDGSFNIDEVSMN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=510, Percent_Identity=58.6274509803922, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=549, Percent_Identity=29.6903460837887, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787762, Length=543, Percent_Identity=26.5193370165746, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787495, Length=510, Percent_Identity=25.0980392156863, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787551, Length=507, Percent_Identity=25.0493096646943, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789887, Length=454, Percent_Identity=24.8898678414097, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789397, Length=470, Percent_Identity=22.9787234042553, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081878, Length=467, Percent_Identity=21.1991434689507, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57569; Mature: 57569

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSLSLPMFRPRALLAAGASVFALTAALPAFAQQTAVIAVDQTFTTMDPYDANDTVSQA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCHHHHHH
AAKSFYEGLFGFDKDMKVVNVLATGYDASPDAKVYTIHLRKGVKFHDGTDFNADAVKANF
HHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCEEEEEEEECCCEECCCCCCCCHHHCCCH
DRVTDPANKLKRYNLFRVIEKTEVVDPYTVKLTLREPFSAIINTLAHPSAVMISPAALKK
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECHHHHHH
WGRDVALHPVGTGPFEFVEWKQTDDLKVKKFAGYWKKGYPKIDALDWKPVIDNNTRAALM
CCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
KAGQADMAFRIPYEQVPDLKSNPKLDIVERPSIVARYVSLNMQQKPFDNLKVRQALNYAI
CCCCCCEEEECCHHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NKEALVKVAFSGFATPSTGVVPPGVEFSTKTGPWPYDPAKARALLKEAGYPNGFETVLWS
CHHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEC
GYNNTTSQKVIQFLQQQLAQVGVKAQVQALESGERVSKVESAPDPATAPVRMFYIGWSAS
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCC
TGEANWALTPLLAAASMPPKLFNTAYYRNPALDDDLSKALQTVDRAKKAELYGDAQKRIW
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
ADAPWIFLVQENIVYARSKRLHGIYVMPDGSFNIDEVSMN
CCCCEEEEEECCEEEECCCCCEEEEEEECCCCCCCEECCC
>Mature Secondary Structure
MNKSLSLPMFRPRALLAAGASVFALTAALPAFAQQTAVIAVDQTFTTMDPYDANDTVSQA
CCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCHHHHHH
AAKSFYEGLFGFDKDMKVVNVLATGYDASPDAKVYTIHLRKGVKFHDGTDFNADAVKANF
HHHHHHHHHHCCCCCHHEEEEEEECCCCCCCCEEEEEEEECCCEECCCCCCCCHHHCCCH
DRVTDPANKLKRYNLFRVIEKTEVVDPYTVKLTLREPFSAIINTLAHPSAVMISPAALKK
HHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECHHHHHH
WGRDVALHPVGTGPFEFVEWKQTDDLKVKKFAGYWKKGYPKIDALDWKPVIDNNTRAALM
CCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
KAGQADMAFRIPYEQVPDLKSNPKLDIVERPSIVARYVSLNMQQKPFDNLKVRQALNYAI
CCCCCCEEEECCHHHCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NKEALVKVAFSGFATPSTGVVPPGVEFSTKTGPWPYDPAKARALLKEAGYPNGFETVLWS
CHHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEC
GYNNTTSQKVIQFLQQQLAQVGVKAQVQALESGERVSKVESAPDPATAPVRMFYIGWSAS
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCC
TGEANWALTPLLAAASMPPKLFNTAYYRNPALDDDLSKALQTVDRAKKAELYGDAQKRIW
CCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHEE
ADAPWIFLVQENIVYARSKRLHGIYVMPDGSFNIDEVSMN
CCCCEEEEEECCEEEECCCCCEEEEEEECCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA