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The map label for this gene is glpK [H]

Identifier: 189352700

GI number: 189352700

Start: 801672

End: 803213

Strand: Direct

Name: glpK [H]

Synonym: BMULJ_03932

Alternate gene names: 189352700

Gene position: 801672-803213 (Clockwise)

Preceding gene: 189352699

Following gene: 189352701

Centisome position: 32.41

GC content: 70.62

Gene sequence:

>1542_bases
ATGACCGAATCCCGAACCGCCATTCTCGCGATCGACGAAGGCACGTCCGGCACGCGTGCCGCGCTCGTCGACGCGAGCGG
ACACGTGTCGTGCCTCGCCTACCTGCCGCTGTCGGTCGACAGCCCGCGACCCGGCGTCGTGGAGCAGGACGCGAACGCGA
TCCTCGACAAGACGCTCGAGGTCTGCCGCGCGACGCTCGCGCAAGCGCGCGAGCAGCGCATGCACATCGTCGCGCTCGCG
CTGGCGACGCAACGCGCGACCGCCGTGCTGTGGGATACCGAGACCGGACGCGCGCTGGTGCCGGCGATGGTCTGGCAGGA
CACGCGCCACGCGGACGAACTCGACCGGCTCGCTGCCGATTGGGACCGCGAACTGCTGCCGTACGTCGGGCGGCCGGCCG
GTGTGCGCTCGCCGTATCTGTGGGCTGCGCACCAGCTGCGCACGTCGCAGCCGGTGGCCGATGCGCATCGCGCGGGCCGG
CTCGCGTTCGGCACGATCGATACCTGGCTGCTGTGGCATCTGTCCGATGCGCGCGTGTGCGTGACGACGCCGACCAATGC
GACGTCCGCGAGCGCTTACCTGCTTGGCGAGCATCGCTATTTCGATCGCTGGATCGATGCGCTGGGTTTCCCGCGCGAGT
TGCTGCCGGCGCTGCGCGAGGACGCCGACGCTTTCGGCCGCACGCGCGCCGACCTGCTCGGCATCGACGTGCCGATCCTC
GCATGCGCGGGCGACCAGTTCGCGGGCGCGATCGGCCTCGGCTGCGTCGAGCGCGGCCACGCGATGTGCGTGCATGGCAC
GGGCAGCTTCGTCGATCTGCTGACGGGCACGACGCGGCCCAATGTCGGCGGCCGGGCGGAGCCCGGCGACGCGCACGACG
GCACGCTCGCGATGACCGCGCGCCGGCACGGCGGCGTGTCGCATTACTCGCTCGAGACCTTCGTCGCAACCACCGGCTCC
GCGTTGCGCTGGCTCTGCGAGAAGCTGCATTGGTTCGACGATCCGGCGCAGATCAGCACGCTCGCGGGCAGCGTGGATTC
GTCGCGCGGCGTGACCTTCGTGCCGGCATTGACGGGCTTGCGCGTGCCGCGAATGGAGCCGAATGCCCGCGCGCTGCTGT
CGGGTATCTCGATCGCGACGACGCAGGCCGAAGTCGCGTACGCGGTACTGGAAGGCATCGCACATTCGGTCACGTCGTGC
ATGGAAGCCAACCAGGCGGTCGCGCGGGCCGACGTGTCCGAGCTGATCGTCGGCGGCGGTCTCGCGGGCAGCGACACGCT
GCTGCAGATCCAGGCCGACGTCAGCGGTGTGCCGGTGCGACGGATCCGCGAGGGCGATCGCGCGAGCCTGCGCGGCGCGG
CGTTTCTCGCGGGTGCCTCCGGGTTGCTGTGGGATTCGCTCGACGCGGCCTGCGCGACGCTCGTCACCGATGCCGTGTTC
GAGCCGTCGATTGATGCGGAGAACCGGCAGCGGCGGCGCGCGGCATGGCATGCGCGGATTGCGTCCGAGCTGGCGCACGC
AGCCGATTTTGCTCATGCGTAA

Upstream 100 bases:

>100_bases
CGACGATCAATCCCGACAACGAGCCCGATGCGCCGCACTTCGACGACGATGCGCGCGCGACATCGACCCTCCTCAAACCC
GCCATCGAGAAAACCCAGCG

Downstream 100 bases:

>100_bases
GCACGGAGAACGACCAGCATGATGTTCCTGCCATCCAGAAAGCCCCGGGCGGACCGGGCCGACATGACGGGCACCGAGCC
GCTGCGGGCGAACCGCGACG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTESRTAILAIDEGTSGTRAALVDASGHVSCLAYLPLSVDSPRPGVVEQDANAILDKTLEVCRATLAQAREQRMHIVALA
LATQRATAVLWDTETGRALVPAMVWQDTRHADELDRLAADWDRELLPYVGRPAGVRSPYLWAAHQLRTSQPVADAHRAGR
LAFGTIDTWLLWHLSDARVCVTTPTNATSASAYLLGEHRYFDRWIDALGFPRELLPALREDADAFGRTRADLLGIDVPIL
ACAGDQFAGAIGLGCVERGHAMCVHGTGSFVDLLTGTTRPNVGGRAEPGDAHDGTLAMTARRHGGVSHYSLETFVATTGS
ALRWLCEKLHWFDDPAQISTLAGSVDSSRGVTFVPALTGLRVPRMEPNARALLSGISIATTQAEVAYAVLEGIAHSVTSC
MEANQAVARADVSELIVGGGLAGSDTLLQIQADVSGVPVRRIREGDRASLRGAAFLAGASGLLWDSLDAACATLVTDAVF
EPSIDAENRQRRRAAWHARIASELAHAADFAHA

Sequences:

>Translated_513_residues
MTESRTAILAIDEGTSGTRAALVDASGHVSCLAYLPLSVDSPRPGVVEQDANAILDKTLEVCRATLAQAREQRMHIVALA
LATQRATAVLWDTETGRALVPAMVWQDTRHADELDRLAADWDRELLPYVGRPAGVRSPYLWAAHQLRTSQPVADAHRAGR
LAFGTIDTWLLWHLSDARVCVTTPTNATSASAYLLGEHRYFDRWIDALGFPRELLPALREDADAFGRTRADLLGIDVPIL
ACAGDQFAGAIGLGCVERGHAMCVHGTGSFVDLLTGTTRPNVGGRAEPGDAHDGTLAMTARRHGGVSHYSLETFVATTGS
ALRWLCEKLHWFDDPAQISTLAGSVDSSRGVTFVPALTGLRVPRMEPNARALLSGISIATTQAEVAYAVLEGIAHSVTSC
MEANQAVARADVSELIVGGGLAGSDTLLQIQADVSGVPVRRIREGDRASLRGAAFLAGASGLLWDSLDAACATLVTDAVF
EPSIDAENRQRRRAAWHARIASELAHAADFAHA
>Mature_512_residues
TESRTAILAIDEGTSGTRAALVDASGHVSCLAYLPLSVDSPRPGVVEQDANAILDKTLEVCRATLAQAREQRMHIVALAL
ATQRATAVLWDTETGRALVPAMVWQDTRHADELDRLAADWDRELLPYVGRPAGVRSPYLWAAHQLRTSQPVADAHRAGRL
AFGTIDTWLLWHLSDARVCVTTPTNATSASAYLLGEHRYFDRWIDALGFPRELLPALREDADAFGRTRADLLGIDVPILA
CAGDQFAGAIGLGCVERGHAMCVHGTGSFVDLLTGTTRPNVGGRAEPGDAHDGTLAMTARRHGGVSHYSLETFVATTGSA
LRWLCEKLHWFDDPAQISTLAGSVDSSRGVTFVPALTGLRVPRMEPNARALLSGISIATTQAEVAYAVLEGIAHSVTSCM
EANQAVARADVSELIVGGGLAGSDTLLQIQADVSGVPVRRIREGDRASLRGAAFLAGASGLLWDSLDAACATLVTDAVFE
PSIDAENRQRRRAAWHARIASELAHAADFAHA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=527, Percent_Identity=31.1195445920304, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI189409120, Length=527, Percent_Identity=31.1195445920304, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI42794763, Length=534, Percent_Identity=30.7116104868914, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI41393575, Length=513, Percent_Identity=30.2144249512671, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=25.6862745098039, Blast_Score=173, Evalue=4e-43,
Organism=Escherichia coli, GI1790361, Length=515, Percent_Identity=31.2621359223301, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789987, Length=460, Percent_Identity=27.1739130434783, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=443, Percent_Identity=29.5711060948081, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=23.9382239382239, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71986147, Length=284, Percent_Identity=26.4084507042254, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321755, Length=473, Percent_Identity=27.4841437632135, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864214, Length=517, Percent_Identity=28.4332688588008, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=28.8590604026846, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24655555, Length=464, Percent_Identity=27.3706896551724, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655560, Length=464, Percent_Identity=27.3706896551724, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655570, Length=464, Percent_Identity=27.3706896551724, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655574, Length=464, Percent_Identity=27.3706896551724, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24655565, Length=464, Percent_Identity=27.3706896551724, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=25.7751937984496, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21357867, Length=533, Percent_Identity=24.765478424015, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922058, Length=522, Percent_Identity=26.2452107279693, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24652871, Length=495, Percent_Identity=26.2626262626263, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24656372, Length=301, Percent_Identity=27.5747508305648, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552909, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54722; Mature: 54591

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESRTAILAIDEGTSGTRAALVDASGHVSCLAYLPLSVDSPRPGVVEQDANAILDKTLE
CCCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
VCRATLAQAREQRMHIVALALATQRATAVLWDTETGRALVPAMVWQDTRHADELDRLAAD
HHHHHHHHHHHHHHHHHEEHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHH
WDRELLPYVGRPAGVRSPYLWAAHQLRTSQPVADAHRAGRLAFGTIDTWLLWHLSDARVC
HCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEECHHHEEEEEECCCEEE
VTTPTNATSASAYLLGEHRYFDRWIDALGFPRELLPALREDADAFGRTRADLLGIDVPIL
EECCCCCCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEE
ACAGDQFAGAIGLGCVERGHAMCVHGTGSFVDLLTGTTRPNVGGRAEPGDAHDGTLAMTA
EECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
RRHGGVSHYSLETFVATTGSALRWLCEKLHWFDDPAQISTLAGSVDSSRGVTFVPALTGL
HHCCCCCEEEHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEHHHCC
RVPRMEPNARALLSGISIATTQAEVAYAVLEGIAHSVTSCMEANQAVARADVSELIVGGG
CCCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LAGSDTLLQIQADVSGVPVRRIREGDRASLRGAAFLAGASGLLWDSLDAACATLVTDAVF
CCCCCEEEEEEECCCCCCHHHHCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
EPSIDAENRQRRRAAWHARIASELAHAADFAHA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TESRTAILAIDEGTSGTRAALVDASGHVSCLAYLPLSVDSPRPGVVEQDANAILDKTLE
CCCCEEEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
VCRATLAQAREQRMHIVALALATQRATAVLWDTETGRALVPAMVWQDTRHADELDRLAAD
HHHHHHHHHHHHHHHHHEEHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHH
WDRELLPYVGRPAGVRSPYLWAAHQLRTSQPVADAHRAGRLAFGTIDTWLLWHLSDARVC
HCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEECHHHEEEEEECCCEEE
VTTPTNATSASAYLLGEHRYFDRWIDALGFPRELLPALREDADAFGRTRADLLGIDVPIL
EECCCCCCCCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEE
ACAGDQFAGAIGLGCVERGHAMCVHGTGSFVDLLTGTTRPNVGGRAEPGDAHDGTLAMTA
EECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
RRHGGVSHYSLETFVATTGSALRWLCEKLHWFDDPAQISTLAGSVDSSRGVTFVPALTGL
HHCCCCCEEEHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEHHHCC
RVPRMEPNARALLSGISIATTQAEVAYAVLEGIAHSVTSCMEANQAVARADVSELIVGGG
CCCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LAGSDTLLQIQADVSGVPVRRIREGDRASLRGAAFLAGASGLLWDSLDAACATLVTDAVF
CCCCCEEEEEEECCCCCCHHHHCCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHC
EPSIDAENRQRRRAAWHARIASELAHAADFAHA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA