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The map label for this gene is fdhB [H]

Identifier: 189352683

GI number: 189352683

Start: 778870

End: 779784

Strand: Direct

Name: fdhB [H]

Synonym: BMULJ_03914

Alternate gene names: 189352683

Gene position: 778870-779784 (Clockwise)

Preceding gene: 189352682

Following gene: 189352684

Centisome position: 31.49

GC content: 65.57

Gene sequence:

>915_bases
ATGGCATTGCAATCGCTGGACATCAAGCGCGTATCGGCCACCACGACGCCGCCGCCCACGGCGCGCGAACCGGTGACCGG
CAGCGTCGCGAAACTGATCGACGTATCGAAGTGCATCGGCTGCAAGGCATGCCAGACGGCATGCATGGAGTGGAACGACC
TACGCGACGAAATCGGCACCAACGTCGGCGTGTACGACAATCCGGCCGACCTCACCGAGCATTCGTGGACGGTCATGCGG
TTCTCCGAATACGAGAACCCGCAAGGCGACCTCGAATGGCTGATCCGCAAGGACGGCTGCATGCACTGCGAGGATCCGGG
CTGTCTGAAGGCGTGCCCGTCGCCGGGCGCGATCGTGCAGTACAACAACGGGATCGTCGATTTCCACGAGGAGAACTGCA
TCGGCTGCGGCTACTGCGTGACGGGCTGCCCGTTCAACGTGCCGCGGATCTCCAAGAAGGATCATCGCGCCTACAAGTGC
ACGCTCTGTTCGGACCGCGTCGCGGTCGGCCAGGAGCCGGCCTGCGTGAAGACGTGTCCGACCGGCGCGATCGTGTTCGG
CACCAAGGAGGACATGAAGCAGCACGCGGCCGAGCGGATCGAGGACCTGAAGGAGCGCGGCTTCGAGCATGCGGGGCTGT
ACGACCCGCAGGGCGTCGGCGGCACGCACGTGATGTACGTGCTGCACCATGCGGACAAACCGTCGCTGTATCACGGGCTG
CCCGACAATCCGTCGATCAGCCCGATGGTGCGGCTGTGGAAGGGCATCGCGAAGCCGCTCGCGGTCGCGGGCCTCGCGCT
GACCGCGCTGGCCGGCTTCTTCCACTACGTGCGCGTGGGCCCGAACGAGGTGACCGACGAAGAGGAAGCCGCCGCCCGCG
ACGAGGCGCGACGCATCAAGGAGGACGCGAAATGA

Upstream 100 bases:

>100_bases
CTCGCCAATACGCTGACTCCGTCCGTGGGTGACGGCAACTCGTACACACCGGAATTCAAGTCGTTTCTGGTGAAGGTCGA
AAAGGCGTAAGGGGGACGAG

Downstream 100 bases:

>100_bases
AGCACGACGACCCGAACCTGATCGTCCGCTACACGGCGAACGAGCGCACGAATCACTGGATCACCGCGATCACGTTCGTG
CTGCTCGCGCTGTCCGGGCT

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLTEHSWTVMR
FSEYENPQGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKC
TLCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERIEDLKERGFEHAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPMVRLWKGIAKPLAVAGLALTALAGFFHYVRVGPNEVTDEEEAAARDEARRIKEDAK

Sequences:

>Translated_304_residues
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLTEHSWTVMR
FSEYENPQGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKC
TLCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERIEDLKERGFEHAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
PDNPSISPMVRLWKGIAKPLAVAGLALTALAGFFHYVRVGPNEVTDEEEAAARDEARRIKEDAK
>Mature_303_residues
ALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEIGTNVGVYDNPADLTEHSWTVMRF
SEYENPQGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQYNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKCT
LCSDRVAVGQEPACVKTCPTGAIVFGTKEDMKQHAAERIEDLKERGFEHAGLYDPQGVGGTHVMYVLHHADKPSLYHGLP
DNPSISPMVRLWKGIAKPLAVAGLALTALAGFFHYVRVGPNEVTDEEEAAARDEARRIKEDAK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=302, Percent_Identity=67.8807947019868, Blast_Score=426, Evalue=1e-121,
Organism=Escherichia coli, GI1787749, Length=292, Percent_Identity=68.8356164383562, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789370, Length=283, Percent_Identity=29.6819787985866, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787122, Length=172, Percent_Identity=34.8837209302326, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787872, Length=172, Percent_Identity=34.8837209302326, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI2367345, Length=173, Percent_Identity=30.635838150289, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510944, Length=163, Percent_Identity=31.2883435582822, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788811, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=28.3236994219653, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787740, Length=169, Percent_Identity=28.9940828402367, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=134, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=174, Percent_Identity=29.8850574712644, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787963, Length=150, Percent_Identity=26, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082180, Length=176, Percent_Identity=27.8409090909091, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789067, Length=183, Percent_Identity=27.8688524590164, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33306; Mature: 33175

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEIGT
CCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
NVGVYDNPADLTEHSWTVMRFSEYENPQGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCEEEECC
TGAIVFGTKEDMKQHAAERIEDLKERGFEHAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPMVRLWKGIAKPLAVAGLALTALAGFFHYVRVGPNEVTDEEEAAARDEARRIK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
EDAK
HCCC
>Mature Secondary Structure 
ALQSLDIKRVSATTTPPPTAREPVTGSVAKLIDVSKCIGCKACQTACMEWNDLRDEIGT
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCC
NVGVYDNPADLTEHSWTVMRFSEYENPQGDLEWLIRKDGCMHCEDPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCCCCCCCCHHCCCCCCCCEEE
YNNGIVDFHEENCIGCGYCVTGCPFNVPRISKKDHRAYKCTLCSDRVAVGQEPACVKTCP
ECCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCEEEECC
TGAIVFGTKEDMKQHAAERIEDLKERGFEHAGLYDPQGVGGTHVMYVLHHADKPSLYHGL
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCC
PDNPSISPMVRLWKGIAKPLAVAGLALTALAGFFHYVRVGPNEVTDEEEAAARDEARRIK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
EDAK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]