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The map label for this gene is metB [H]

Identifier: 189352666

GI number: 189352666

Start: 756030

End: 757220

Strand: Direct

Name: metB [H]

Synonym: BMULJ_03897

Alternate gene names: 189352666

Gene position: 756030-757220 (Clockwise)

Preceding gene: 189352665

Following gene: 189352670

Centisome position: 30.57

GC content: 65.49

Gene sequence:

>1191_bases
ATGGACGACTCCCTCAACTTCGACACGCTCGCCGTGCGCGCGGGCACGCTGCGCACCGACTTCAACGAGCATTCGGAAGC
GCTGTTCCTGACCTCGAGCTTCTGCTTCAAGAACGCGGCCGAAGCGGCCGAGCGCTTCGCGAATTCGGAAGACTACTTCA
CCTATTCGCGCTTCACGAACCCGACCGTCACGATGTTCCAGGAGCGCCTGGCCGCGCTCGAAGGCGGCGAGGCGTGCATC
GCGACCGCGTCGGGGATGGCCGCGATCATGTCGGTCGTGATGGCCGCGCTGCAGGCGGGCGATCACCTCGTCAGCTCGCG
CAGCCTGTTCGGCTCGACGCTCGGCATGTTCTCGCAGATCTTCAGCAAGTTCGGCATCACGACGACCTTCGTCGATCCGA
CCGATCTGAACGCGTGGAAGGAAGCCGTGCGTCCGGAGACGAAGATGTTCTTCCTCGAGACGCCGTCGAATCCGCTGACC
GAACTCGCCGACATCGAGGCGATCGGCAAGATCGCCAAGGATGCGAACGCGCTGTTCGTCGTCGACAACTGTTTCTGCAG
CCCCGTGCTGCAGCAGCCGCTGAAGCTCGGCGCGGACGTCGTCATGCATTCGGCGACGAAATTCCTCGACGGCCAGGGCC
GCGTGCTCGGCGGCGCGCTGGTCGGCTCGAAGGCATTCATCATGGGCAAGGTGTTCCCGTTCGTGCGCAGCGCGGGCCCG
ACGCTGTCGGCGTTCAACGCGTGGGTGCTGCTGAAGGGGATGGAAACGCTGTCGCTGCGTGTCGAGAAGCAGTCGGCGAA
CGCACTCGAGATCGCGCGCTGGCTCGATTCGCATCCGGCGGTCGCGCGCGTGTTCTATCCGGGCCTCGAATCGCATCCGC
AGCATGAACTCGCGAAGCGCCAGCAGAAGGCAGGCGGCGCGATCGTCTCGTTCGAGCTGAAGGGCGACACGCCCGAGCAG
CAGCGCGCGAACGCATGGCGCGTGATCGACAACACGAAGCTGATTTCGATCACGGGCAATCTCGGCGATACGCGCACGAC
GATCACGCATCCGGCGACGACGACCCATGCACGCATCACGCCCGAAGCGCGTGCGGCGGCGGGGATTACCGAAGGGCTGA
TCCGGCTCGCGGTCGGCCTCGAGAACGCGGGCGACCTGCGCAACGATCTCGCGCGCGGCCTCGAAGGCTGA

Upstream 100 bases:

>100_bases
CTGTCGTACCGCACCGAAGCCCGCTGATGCAGACGCATGGCGGGCTTTTTGTTTGGCCTGGCCCGCGCGTACGCGCGGCC
ATCGAATACGGAAAACGGAC

Downstream 100 bases:

>100_bases
GCGCGGCAACGGCCCAGCGCATCGACGACACGCGCCGTTCGCGCCGGCCTATGCCGGTGCGAACGGCGCGTTCGTTTTAC
TGCCGGCTCGGTTGCGGCCG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MDDSLNFDTLAVRAGTLRTDFNEHSEALFLTSSFCFKNAAEAAERFANSEDYFTYSRFTNPTVTMFQERLAALEGGEACI
ATASGMAAIMSVVMAALQAGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLNAWKEAVRPETKMFFLETPSNPLT
ELADIEAIGKIAKDANALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFIMGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVARVFYPGLESHPQHELAKRQQKAGGAIVSFELKGDTPEQ
QRANAWRVIDNTKLISITGNLGDTRTTITHPATTTHARITPEARAAAGITEGLIRLAVGLENAGDLRNDLARGLEG

Sequences:

>Translated_396_residues
MDDSLNFDTLAVRAGTLRTDFNEHSEALFLTSSFCFKNAAEAAERFANSEDYFTYSRFTNPTVTMFQERLAALEGGEACI
ATASGMAAIMSVVMAALQAGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLNAWKEAVRPETKMFFLETPSNPLT
ELADIEAIGKIAKDANALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFIMGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVARVFYPGLESHPQHELAKRQQKAGGAIVSFELKGDTPEQ
QRANAWRVIDNTKLISITGNLGDTRTTITHPATTTHARITPEARAAAGITEGLIRLAVGLENAGDLRNDLARGLEG
>Mature_396_residues
MDDSLNFDTLAVRAGTLRTDFNEHSEALFLTSSFCFKNAAEAAERFANSEDYFTYSRFTNPTVTMFQERLAALEGGEACI
ATASGMAAIMSVVMAALQAGDHLVSSRSLFGSTLGMFSQIFSKFGITTTFVDPTDLNAWKEAVRPETKMFFLETPSNPLT
ELADIEAIGKIAKDANALFVVDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFIMGKVFPFVRSAGP
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVARVFYPGLESHPQHELAKRQQKAGGAIVSFELKGDTPEQ
QRANAWRVIDNTKLISITGNLGDTRTTITHPATTTHARITPEARAAAGITEGLIRLAVGLENAGDLRNDLARGLEG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=37.8048780487805, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=34.4512195121951, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=32.4159021406728, Blast_Score=175, Evalue=7e-44,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=37.4677002583979, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789383, Length=351, Percent_Identity=29.9145299145299, Blast_Score=157, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17560056, Length=381, Percent_Identity=35.4330708661417, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=381, Percent_Identity=34.9081364829396, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=34.1397849462366, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=33.6405529953917, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=403, Percent_Identity=31.0173697270471, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=27.9411764705882, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=314, Percent_Identity=26.1146496815287, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=347, Percent_Identity=26.8011527377522, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=36.5994236311239, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42756; Mature: 42756

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSLNFDTLAVRAGTLRTDFNEHSEALFLTSSFCFKNAAEAAERFANSEDYFTYSRFTN
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PTVTMFQERLAALEGGEACIATASGMAAIMSVVMAALQAGDHLVSSRSLFGSTLGMFSQI
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FSKFGITTTFVDPTDLNAWKEAVRPETKMFFLETPSNPLTELADIEAIGKIAKDANALFV
HHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
VDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFIMGKVFPFVRSAGP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCC
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVARVFYPGLESHPQHELAKR
CHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
QQKAGGAIVSFELKGDTPEQQRANAWRVIDNTKLISITGNLGDTRTTITHPATTTHARIT
HHHCCCEEEEEEECCCCCHHHHCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCEEEEC
PEARAAAGITEGLIRLAVGLENAGDLRNDLARGLEG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MDDSLNFDTLAVRAGTLRTDFNEHSEALFLTSSFCFKNAAEAAERFANSEDYFTYSRFTN
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCC
PTVTMFQERLAALEGGEACIATASGMAAIMSVVMAALQAGDHLVSSRSLFGSTLGMFSQI
CHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
FSKFGITTTFVDPTDLNAWKEAVRPETKMFFLETPSNPLTELADIEAIGKIAKDANALFV
HHHHCCEEEEECCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEE
VDNCFCSPVLQQPLKLGADVVMHSATKFLDGQGRVLGGALVGSKAFIMGKVFPFVRSAGP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHHHHHHHHHHHCCCC
TLSAFNAWVLLKGMETLSLRVEKQSANALEIARWLDSHPAVARVFYPGLESHPQHELAKR
CHHHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHH
QQKAGGAIVSFELKGDTPEQQRANAWRVIDNTKLISITGNLGDTRTTITHPATTTHARIT
HHHCCCEEEEEEECCCCCHHHHCCCEEEECCCEEEEEECCCCCCCEEEECCCCCCEEEEC
PEARAAAGITEGLIRLAVGLENAGDLRNDLARGLEG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]