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The map label for this gene is purF [H]

Identifier: 189352665

GI number: 189352665

Start: 754265

End: 755797

Strand: Direct

Name: purF [H]

Synonym: BMULJ_03896

Alternate gene names: 189352665

Gene position: 754265-755797 (Clockwise)

Preceding gene: 189352664

Following gene: 189352666

Centisome position: 30.5

GC content: 64.77

Gene sequence:

>1533_bases
ATGTGCGGCATCGTAGGCGTTATCTCCCAATCCCCGGTCAACCAGCTGATCTATGACAGCCTGCTGCTGCTGCAGCATCG
CGGTCAGGACGCGGCAGGCATCGCGACGGCGGACGGCAGCAATTTCCACATGTACAAGGCGAACGGCATGGTGCGCGACG
TGTTCCGCACGCGTAACATGCGCAGCCTGCCCGGCACGTTCGGGATCGGCCAGGTCCGCTATCCGACGGCCGGCTCCGCA
TCGAGCGAAGCCGAGGCGCAGCCGTTCTACGTGAACGCACCGTTCGGGATCATCCTCGCGCACAACGGCAACCTGACGAA
CTGGCAACAACTGAAGGACGAGATGTTCCGGATCGACCGCCGGCACATCAACACCAATTCCGACAGCGAAGTGCTGCTCA
ACGTGTTCGCGCACGAACTGCAGTTGTCGACGACGGGCCTCGAGCTCGATCCGGCCGCGCTGTTCAAGGCGGTTGCGGGC
GTGCACCGCCGCGTGCACGGCTCGTATGCGATCGTCTCGCTGATCGCCGGCTACGGTCTGCTCGCGTTCCGCGATCCGTT
CGGCATCCGCCCGCTCTGCATCGGCAAGCTCGAGACCGAGCACGGCACCGAATGGATGGTCGCGTCGGAGTCGGTCGCGG
TCGAAGGCATCGGCTTCGAGTTCGTGCGCGACGTGCTGCCGGGCGAAGCGATCTTCATCGACCAGGCGGGCAACTTTCAC
AGCCAGCAGTGCGCGGACAATCCGACGCTGAATCCGTGCATGTTCGAGTACGTCTATCTCGCGCGTCCGGACTCGTGCCT
CGACGGCGTGCCCGTCTACAACGTGCGCCTGCGCATGGGCGACTATCTCGCCGAGAAGATCAAGCGCGAGCTGCCGAACG
TGCCGATCGACGTCGTGATGCCGATTCCCGATTCGTCGCGCCCGGCCGCGATGCAGGTCGCCGCGAAGCTCGGCGTCGAG
TATCGCGAAGGCTTCTTCAAGAACCGCTACGTCGGCCGCACGTTCATCATGCCGGGGCAGGCGGTGCGCAAGAAGTCGGT
GCGCCAGAAGCTCAACGCGATGAGCATCGAGTTCAAGGACAAGAACGTGCTGATCGTCGACGATTCGATCGTGCGCGGCA
CGACGTCGCACGAGATCGTGCAGATGGCGCGCGATGCGGGCGCGAAGTCGGTGATTTTCGCGTCGGCGGCGCCGCCCGTG
AAATTCCCGAACGTGTACGGCATCGACATGCCGACGCGCGGCGAACTCGTCGCGCACGGCCGCAGCGACGAGGAAGTCGC
GAAGATCATCGGCGCCGACCATCTGATCTATCAGGACGTCGACGATCTGCGCCGCGCGGTGCGCGACATCAACCCGAAGC
TCGAGCGCTTCGAGGCGTCGTGCTTCGACGGCAACTACATCACCGGCGACGTGACGCCCGAGTATCTCGATGCGATCGAG
CGCGCGCGCCTGACGCCGGCATCGCAGGCCGACCGCGACACGGCGAGCGATACCGCACGGTCGCAGATGAACCTGCAGCT
GTCGGTCGAGTGA

Upstream 100 bases:

>100_bases
CTGAAGCAGCTCCTGCCCGACGGCATGGCCCAGTACGTGCGCGTGTGAGCGTACGCACGGGGCAGGACGGAACCGATTTT
GTCATTCTGAAGGACATGCC

Downstream 100 bases:

>100_bases
TTGCCGGCGCACGCTTGTGCCGAAGCGTGCTAGGATGTGGCCTTGCGTCGATTTGATTTCAGCTTGCGGACGCGGGGCTT
CTTCCCAAAACAGCTAAAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MCGIVGVISQSPVNQLIYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTFGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLSTTGLELDPAALFKAVAG
VHRRVHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEHGTEWMVASESVAVEGIGFEFVRDVLPGEAIFIDQAGNFH
SQQCADNPTLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKNVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRSDEEVAKIIGADHLIYQDVDDLRRAVRDINPKLERFEASCFDGNYITGDVTPEYLDAIE
RARLTPASQADRDTASDTARSQMNLQLSVE

Sequences:

>Translated_510_residues
MCGIVGVISQSPVNQLIYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTFGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLSTTGLELDPAALFKAVAG
VHRRVHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEHGTEWMVASESVAVEGIGFEFVRDVLPGEAIFIDQAGNFH
SQQCADNPTLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKNVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRSDEEVAKIIGADHLIYQDVDDLRRAVRDINPKLERFEASCFDGNYITGDVTPEYLDAIE
RARLTPASQADRDTASDTARSQMNLQLSVE
>Mature_510_residues
MCGIVGVISQSPVNQLIYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTFGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVFAHELQLSTTGLELDPAALFKAVAG
VHRRVHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEHGTEWMVASESVAVEGIGFEFVRDVLPGEAIFIDQAGNFH
SQQCADNPTLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKNVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRSDEEVAKIIGADHLIYQDVDDLRRAVRDINPKLERFEASCFDGNYITGDVTPEYLDAIE
RARLTPASQADRDTASDTARSQMNLQLSVE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=37.3417721518987, Blast_Score=275, Evalue=6e-74,
Organism=Escherichia coli, GI1788651, Length=494, Percent_Identity=58.9068825910931, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1790167, Length=199, Percent_Identity=30.1507537688442, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=495, Percent_Identity=33.9393939393939, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6323958, Length=499, Percent_Identity=50.501002004008, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI28573187, Length=505, Percent_Identity=36.8316831683168, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24659598, Length=505, Percent_Identity=35.8415841584158, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24659604, Length=490, Percent_Identity=36.530612244898, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56331; Mature: 56331

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVISQSPVNQLIYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHCCC
RSLPGTFGIGQVRYPTAGSASSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHELQLSTTGLELDPAALFKAVAGVHRRVHGSYAIVSLIAGYGL
HHCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCE
LAFRDPFGIRPLCIGKLETEHGTEWMVASESVAVEGIGFEFVRDVLPGEAIFIDQAGNFH
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHHHCCCCEEEEECCCCCC
SQQCADNPTLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELPNVPIDVVM
CHHCCCCCCCCHHHHHEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KNVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRGELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RSDEEVAKIIGADHLIYQDVDDLRRAVRDINPKLERFEASCFDGNYITGDVTPEYLDAIE
CCHHHHHHHHCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHH
RARLTPASQADRDTASDTARSQMNLQLSVE
HHCCCCCCCCCCCCHHHHHHHHEEEEEEEC
>Mature Secondary Structure
MCGIVGVISQSPVNQLIYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHCCC
RSLPGTFGIGQVRYPTAGSASSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHELQLSTTGLELDPAALFKAVAGVHRRVHGSYAIVSLIAGYGL
HHCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCE
LAFRDPFGIRPLCIGKLETEHGTEWMVASESVAVEGIGFEFVRDVLPGEAIFIDQAGNFH
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHHHCCCCEEEEECCCCCC
SQQCADNPTLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIKRELPNVPIDVVM
CHHCCCCCCCCHHHHHEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KNVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRGELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RSDEEVAKIIGADHLIYQDVDDLRRAVRDINPKLERFEASCFDGNYITGDVTPEYLDAIE
CCHHHHHHHHCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHH
RARLTPASQADRDTASDTARSQMNLQLSVE
HHCCCCCCCCCCCCHHHHHHHHEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]