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The map label for this gene is leuC [H]

Identifier: 189352650

GI number: 189352650

Start: 738392

End: 739801

Strand: Direct

Name: leuC [H]

Synonym: BMULJ_03881

Alternate gene names: 189352650

Gene position: 738392-739801 (Clockwise)

Preceding gene: 189352649

Following gene: 189352651

Centisome position: 29.86

GC content: 66.17

Gene sequence:

>1410_bases
ATGGCACAGACTCTCTACGACAAACTGTGGAATTCCCACGTCGTCCACACCGAGGAAGACGGCACGGCATTGCTGTATAT
CGACCGTCAACTGCTGCATGAAGTCACGAGCCCGCAGGCGTTCGAAGGGCTGAAGATCGCGCAGCGTCCGGTGTGGCGCA
TCAGCGCGAACCTGGCCGTGTCCGACCACAACGTGCCGACCACCGATCGCAGCCACGGCATCGCCGATCCCGTCTCGAAG
CTGCAGGTCGACACGCTCGACGCGAACTGCGATGCGTTCGGCATCACGCAGTTCAAGATGAACGACGTGCGTCAGGGCAT
CGTGCACATCATCGGGCCGGAGCAGGGCGCGACGCTGCCGGGCATGACGATCGTGTGCGGCGATTCGCACACGTCGACGC
ACGGCGCGTTCGGCGCGCTCGCGCACGGCATCGGCACGTCGGAAGTCGAGCACGTGCTCGCGACGCAGACTTTGCTGCAG
AAGAAGAGCAAGAACATGCTCGTGAAGGTCGAGGGCGCACTGCCGCGCGGCTGCACCGCGAAGGACATCGTGCTCGCGAT
CATCGGCAAGATCGGCACGGCAGGCGGCACCGGCTATGCGATCGAATTCGGCGGCTCGACGATCCGCGCGCTGTCGATGG
AAGGCCGGATGACGGTCTGCAACATGGCGATCGAAGCCGGCGCGCGCGCGGGCATGGTCGCCGTCGACGACACGACGATC
GACTACCTGAAGGGCCGTCCGTTCGTGCCGACCGGCGCGGAATGGGATCAGGCCGTCGAATACTGGCGCCAGTTCAAGTC
GGACGAAGGCGCGCATTTCGATCGCGTCGTCGAGCTGAACGCGGCCGAGATCGTCCCGCAGGTCACGTGGGGCACGTCGC
CGGAAATGGTCACGTCGATCGACGGTCGCGTGCCCGATCCCGAGCGCGAGAAGGATCCGGTCAAGCGCGACGCGATGGAG
CGCGCGCTCGCCTACATGGCGCTCGAACCGAACACGCCGATCGAATCGATCAAGGTCGACAAGATCTTCATCGGCTCGTG
CACGAACGCGCGCATCGAGGACATCCGCGCGGCCGCGTACGTCGTGAAGAAGCTGAACCGTCGCGTCGCCTCGAACGTGC
GGCTCGCGATGGTCGTGCCGGGCTCGGGCCTCGTGAAGGCGCAGGCCGAGCGCGAAGGCCTCGACAAGGTGTTTACCGAT
GCGGGCTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGTCTCGCGATGAACGCCGACCGCCTCGAGCCGGGCGAGCGCTG
CGCCTCGACGTCGAACCGCAACTTCGAAGGCCGGCAGGGCGCGGGCGGCCGCACGCACCTCGTCAGCCCCGCGATGGCGG
CGGCCGCGGCGATCGAAGGCCATTTCGTCGACATTCGCAAGCTGGGGTAA

Upstream 100 bases:

>100_bases
TTAATGGGTTTTTCGGCGTTTCGCGTGCGCCGTTGCGCGTTCTGCGTGGCATAATCGACCCCATCCGCAAGGCTTGATGT
CACAACGACCCCGCATACCC

Downstream 100 bases:

>100_bases
CGCGTGACGGCATCGAAATGGGTTGCGCGGCTCGCCGCCGCGCTGGCGCTCGCGGGGCTCGGCTTCGGTCTCGCGGGCTG
CAACACCGTCCGAGGCTTCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKIAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDAFGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNMLVKVEGALPRGCTAKDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTI
DYLKGRPFVPTGAEWDQAVEYWRQFKSDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAME
RALAYMALEPNTPIESIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVASNVRLAMVVPGSGLVKAQAEREGLDKVFTD
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Sequences:

>Translated_469_residues
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKIAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDAFGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQ
KKSKNMLVKVEGALPRGCTAKDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTI
DYLKGRPFVPTGAEWDQAVEYWRQFKSDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAME
RALAYMALEPNTPIESIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVASNVRLAMVVPGSGLVKAQAEREGLDKVFTD
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
>Mature_468_residues
AQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKIAQRPVWRISANLAVSDHNVPTTDRSHGIADPVSKL
QVDTLDANCDAFGITQFKMNDVRQGIVHIIGPEQGATLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQK
KSKNMLVKVEGALPRGCTAKDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTID
YLKGRPFVPTGAEWDQAVEYWRQFKSDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTSIDGRVPDPEREKDPVKRDAMER
ALAYMALEPNTPIESIKVDKIFIGSCTNARIEDIRAAAYVVKKLNRRVASNVRLAMVVPGSGLVKAQAEREGLDKVFTDA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=28.6501377410468, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI8659555, Length=413, Percent_Identity=26.3922518159806, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=27.1739130434783, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.2478632478632, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=379, Percent_Identity=26.9129287598945, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32564738, Length=364, Percent_Identity=29.9450549450549, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=29.9450549450549, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=29.1390728476821, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=26.9135802469136, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.8305084745763, Blast_Score=625, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6320440, Length=352, Percent_Identity=29.5454545454545, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=30.3030303030303, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=27.6243093922652, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=396, Percent_Identity=27.2727272727273, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17864292, Length=396, Percent_Identity=27.2727272727273, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI161076999, Length=396, Percent_Identity=27.2727272727273, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=27.0440251572327, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=26.4550264550265, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=25.5376344086022, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50689; Mature: 50558

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKIAQRPVWRISANLAV
CCHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAFGITQFKMNDVRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGALPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
KDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTI
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEHHHHHCCCCCCEEEECCCHH
DYLKGRPFVPTGAEWDQAVEYWRQFKSDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCEEEECCCCCCCEEEC
DGRVPDPEREKDPVKRDAMERALAYMALEPNTPIESIKVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVASNVRLAMVVPGSGLVKAQAEREGLDKVFTDAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AQTLYDKLWNSHVVHTEEDGTALLYIDRQLLHEVTSPQAFEGLKIAQRPVWRISANLAV
CHHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHHCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDAFGITQFKMNDVRQGIVHIIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEEEEHHHHHCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLQKKSKNMLVKVEGALPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
KDIVLAIIGKIGTAGGTGYAIEFGGSTIRALSMEGRMTVCNMAIEAGARAGMVAVDDTTI
HHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEHHHHHCCCCCCEEEECCCHH
DYLKGRPFVPTGAEWDQAVEYWRQFKSDEGAHFDRVVELNAAEIVPQVTWGTSPEMVTSI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCEEEECCCCCCCEEEC
DGRVPDPEREKDPVKRDAMERALAYMALEPNTPIESIKVDKIFIGSCTNARIEDIRAAAY
CCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHEEEEEEEEECCCCCHHHHHHHHHH
VVKKLNRRVASNVRLAMVVPGSGLVKAQAEREGLDKVFTDAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA